Directed Evolution: Bringing New Chemistry to Life

Angewandte Chemie International Edition, Journal Year: 2017, Volume and Issue: 57(16), P. 4143 - 4148

Published: Oct. 24, 2017

Tailor-made: Discussed herein is the ability to adapt biology's mechanisms for innovation and optimization solving problems in chemistry engineering. The evolution of nature's enzymes can lead discovery new reactivity, transformations not known biology, reactivity inaccessible by small-molecule catalysts.

Language: Английский

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Biocatalysis

Nature Reviews Methods Primers, Journal Year: 2021, Volume and Issue: 1(1)

Published: June 24, 2021

Language: Английский

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Biocatalytic Oxidation Reactions: A Chemist's Perspective

Angewandte Chemie International Edition, Journal Year: 2018, Volume and Issue: 57(30), P. 9238 - 9261

Published: March 24, 2018

Abstract Oxidation chemistry using enzymes is approaching maturity and practical applicability in organic synthesis. Oxidoreductases (enzymes catalysing redox reactions) enable chemists to perform highly selective efficient transformations ranging from simple alcohol oxidations stereoselective halogenations of non‐activated C−H bonds. For many these reactions, no “classical” chemical counterpart known. Hence oxidoreductases open up shorter synthesis routes based on a more direct access the target products. The generally very mild reaction conditions may also reduce environmental impact biocatalytic reactions compared classical counterparts. In this Review, we critically summarise most important recent developments field oxidation identify pressing bottlenecks as well promising solutions.

Language: Английский

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The Crucial Role of Methodology Development in Directed Evolution of Selective Enzymes

Angewandte Chemie International Edition, Journal Year: 2019, Volume and Issue: 59(32), P. 13204 - 13231

Published: July 3, 2019

Directed evolution of stereo-, regio-, and chemoselective enzymes constitutes a unique way to generate biocatalysts for synthetically interesting transformations in organic chemistry biotechnology. In order this protein engineering technique be efficient, fast, reliable, also relevance synthetic chemistry, methodology development was still is necessary. Following description early key contributions, review focuses on recent developments. It includes optimization molecular biological methods gene mutagenesis the design efficient strategies their application, resulting notable reduction screening effort (bottleneck directed evolution). When aiming laboratory selectivity activity, second-generation versions Combinatorial Active-Site Saturation Test (CAST) Iterative Mutagenesis (ISM), both involving saturation (SM) at sites lining binding pocket, have emerged as preferred approaches, aided by silico such machine learning. The recently proposed Focused Rational Site-specific (FRISM) fusion rational evolution. On-chip solid-phase chemical synthesis rapid library construction enhances quality notably eliminating undesired amino acid bias, future evolution?

Language: Английский

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Extending the application of biocatalysis to meet the challenges of drug development

Nature Reviews Chemistry, Journal Year: 2018, Volume and Issue: 2(12), P. 409 - 421

Published: Nov. 20, 2018

Language: Английский

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The Hydrophobic Effect Applied to Organic Synthesis: Recent Synthetic Chemistry “in Water”

Chemistry - A European Journal, Journal Year: 2018, Volume and Issue: 24(26), P. 6672 - 6695

Published: Feb. 21, 2018

Recent developments over the past few years in aqueous micellar catalysis are discussed. Applications to problems synthesis highlighted, enabled by use of surfactants that self-aggregate water into micelles as nanoreactors. These include amphiphiles have been available for some time, well those newly designed. Reactions catalyzed transition metals, including Pd, Cu, Rh, and Au, particular focus.

Language: Английский

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Power of Biocatalysis for Organic Synthesis

ACS Central Science, Journal Year: 2021, Volume and Issue: 7(1), P. 55 - 71

Published: Jan. 14, 2021

Biocatalysis, using defined enzymes for organic transformations, has become a common tool in synthesis, which is also frequently applied industry. The generally high activity and outstanding stereo-, regio-, chemoselectivity observed many biotransformations are the result of precise control reaction active site biocatalyst. This achieved by exact positioning reagents relative to each other fine-tuned 3D environment, specific activating interactions between protein, subtle movements catalyst. Enzyme engineering enables one adapt catalyst desired process. A well-filled biocatalytic toolbox ready be used various reactions. Providing nonnatural conditions evolving biocatalysts play with myriad options creating novel transformations thereby opening new, short pathways target molecules. Combining several pot perform reactions concurrently increases efficiency biocatalysis even further.

Language: Английский

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Broadening the Scope of Biocatalysis in Sustainable Organic Synthesis

ChemSusChem, Journal Year: 2019, Volume and Issue: 12(13), P. 2859 - 2881

Published: April 1, 2019

Abstract This Review is aimed at synthetic organic chemists who may be familiar with organometallic catalysis but have no experience biocatalysis, and seeks to provide an answer the perennial question: if it so attractive, why wasn't extensively used in past? The development of biocatalysis industrial synthesis traced from middle last century. Advances molecular biology two decades, particular genome sequencing, gene directed evolution proteins, enabled remarkable improvements scope substantially reduced biocatalyst times cost contributions. Additionally, recovery reuse been facilitated by developments enzyme immobilization technologies. Biocatalysis has become eminently competitive chemocatalysis biocatalytic production important pharmaceutical intermediates, such as enantiopure alcohols amines, mainstream synthesis. space significantly expanded currently being extended even further include new‐to‐nature reactions.

Language: Английский

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Genetically programmed chiral organoborane synthesis

Nature, Journal Year: 2017, Volume and Issue: 552(7683), P. 132 - 136

Published: Nov. 28, 2017

Language: Английский

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Embracing Nature’s Catalysts: A Viewpoint on the Future of Biocatalysis

ACS Catalysis, Journal Year: 2020, Volume and Issue: 10(15), P. 8418 - 8427

Published: June 4, 2020

ADVERTISEMENT RETURN TO ISSUEPREVViewpointNEXTEmbracing Nature's Catalysts: A Viewpoint on the Future of BiocatalysisBernhard Hauer*Bernhard HauerInstitute Biochemistry and Technical Biochemistry, Department Universitaet Stuttgart, Allmandring 31, 70569 Germany*Email: [email protected]More by Bernhard Hauerhttp://orcid.org/0000-0001-6259-3348Cite this: ACS Catal. 2020, 10, 15, 8418–8427Publication Date (Web):June 4, 2020Publication History Received16 April 2020Published online4 June inissue 7 August 2020https://pubs.acs.org/doi/10.1021/acscatal.0c01708https://doi.org/10.1021/acscatal.0c01708article-commentaryACS PublicationsCopyright © 2020 American Chemical Society. This publication is available under these Terms Use. Request reuse permissions free to access through this site. Learn MoreArticle Views15099Altmetric-Citations190LEARN ABOUT THESE METRICSArticle Views are COUNTER-compliant sum full text article downloads since November 2008 (both PDF HTML) across all institutions individuals. These metrics regularly updated reflect usage leading up last few days.Citations number other articles citing article, calculated Crossref daily. Find more information about citation counts.The Altmetric Attention Score a quantitative measure attention that research has received online. Clicking donut icon will load page at altmetric.com with additional details score social media presence for given article. how calculated. Share Add toView InAdd Full Text ReferenceAdd Description ExportRISCitationCitation abstractCitation referencesMore Options onFacebookTwitterWechatLinked InRedditEmail (1 MB) Get e-AlertscloseSUBJECTS:Biocatalysis,Catalysts,Chemical reactions,Industrial manufacturing,Peptides proteins e-Alerts

Language: Английский

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