Clinical Microbiology and Infection, Journal Year: 2022, Volume and Issue: 29(6), P. 682 - 688
Published: Oct. 1, 2022
Language: Английский
Viruses, Journal Year: 2023, Volume and Issue: 15(4), P. 1020 - 1020
Published: April 21, 2023
In response to the global spread of antimicrobial resistance, there is an increased demand for novel and innovative antimicrobials. Bacteriophages have been known their potential clinical utility in lysing bacteria almost a century. Social pressures concomitant introduction antibiotics mid-1900s hindered widespread adoption these naturally occurring bactericides. Recently, however, phage therapy has re-emerged as promising strategy combatting resistance. A unique mechanism action cost-effective production promotes phages ideal solution addressing antibiotic-resistant bacterial infections, particularly lower- middle-income countries. As number phage-related research labs worldwide continues grow, it will be increasingly important encourage expansion well-developed trials, standardization storage cocktails, advancement international collaboration. this review, we discuss history, benefits, limitations bacteriophage its current role setting resistance with specific focus on active trials case reports administration.
Language: Английский
Citations
94
Nature Reviews Microbiology, Journal Year: 2023, Volume and Issue: 22(5), P. 262 - 275
Published: Dec. 11, 2023
Language: Английский
Citations
90
Molecules, Journal Year: 2022, Volume and Issue: 27(6), P. 1857 - 1857
Published: March 13, 2022
Bacterial infectious diseases cause serious harm to human health. At present, antibiotics are the main drugs used in treatment of bacterial diseases, but abuse has led rapid increase drug-resistant bacteria and inability effectively control infections. Bacteriophages a kind virus that infects archaea, adopting as their hosts. The use bacteriophages antimicrobial agents is an alternative antibiotics. phage therapy (PT) been various fields provided new technology for addressing caused by infections humans, animals, plants. PT uses infect pathogenic so stop treat prevent related diseases. However, several limitations, due narrow host range, lysogenic phenomenon, lack relevant policies, pharmacokinetic data. development reasonable strategies overcome these limitations essential further this technology. This review article described current applications summarizes existing solutions limitations. information will be useful clinicians, people working agriculture industry, basic researchers.
Language: Английский
Citations
79
Annual Review of Virology, Journal Year: 2023, Volume and Issue: 10(1), P. 503 - 524
Published: June 2, 2023
The global rise of antibiotic resistance in bacterial pathogens and the waning efficacy antibiotics urge consideration alternative antimicrobial strategies. Phage therapy is a classic approach where bacteriophages (bacteria-specific viruses) are used against infections, with many recent successes personalized medicine treatment intractable infections. However, perpetual challenge for developing generalized phage expectation that viruses will exert selection target bacteria to deploy defenses virus attack, causing evolution during patient treatment. Here we review two main complementary strategies mitigating therapy: minimizing ability populations evolve driving (steering) phage-resistant toward clinically favorable outcomes. We discuss future research directions might further address phage-resistance problem, foster widespread development deployment therapeutic outsmart evolved clinical settings.
Language: Английский
Citations
62
International Journal of Food Science, Journal Year: 2023, Volume and Issue: 2023, P. 1 - 26
Published: Sept. 11, 2023
Salmonella is a foodborne zoonotic pathogen causing diarrhoeal disease to humans after consuming contaminated water, animal, and plant products. The bacterium the third leading cause of human death among diseases worldwide. Therefore, salmonellosis public health concern demanding integrated interventions against causative agent, enterica. prevention in intricate due several factors, including an immune-stable individual infected with S. enterica continuing shed live bacteria without showing any clinical signs. Similarly, asymptomatic animals are source food Furthermore, products animal origin menace industries biofilms, which enhance colonization, persistence, survival on equipment. resulting from equipment offset economic competition partner institutions international business. most worldwide prevalent broad-range serovars affecting Typhimurium Enteritidis, poultry products, others, primary infection. broader range creates over multiple strategies for preventing controlling contamination foods safety humans. Among spread include biosecurity measures, isolation quarantine, epidemiological surveillance, farming systems, herbs spices, vaccination. Other measures application phages, probiotics, prebiotics, nanoparticles reduced capped antimicrobial agents. Salmonella-free such as beef, pork, meat, eggs, milk, foods, vegetables fruits, will prevent This review explains infection caused by quality
Language: Английский
Citations
60
ACS Infectious Diseases, Journal Year: 2023, Volume and Issue: 9(7), P. 1283 - 1302
Published: June 22, 2023
The emergence of antibiotic-resistant bacterial strains is a source public health concern across the globe. As discovery new conventional antibiotics has stalled significantly over past decade, there an urgency to develop novel approaches address drug resistance in infectious diseases. use CRISPR-Cas-based system for precise elimination targeted populations holds promise as innovative approach antimicrobial agent design. CRISPR-Cas targeting celebrated its high versatility and specificity, offering excellent opportunity fight antibiotic pathogens by selectively inactivating genes involved resistance, biofilm formation, pathogenicity, virulence, or viability. strategy can enact effects two approaches: inactivation chromosomal curing plasmids encoding resistance. In this Review, we provide overview main systems utilized creation these antimicrobials, well highlighting promising studies field. We also offer detailed discussion about most commonly used mechanisms delivery: bacteriophages, nanoparticles, conjugative plasmids. Lastly, possible interference that should be considered during intelligent design approaches.
Language: Английский
Citations
57
Nature Reviews Clinical Oncology, Journal Year: 2024, Volume and Issue: 21(8), P. 569 - 589
Published: June 5, 2024
Language: Английский
Citations
48
Viruses, Journal Year: 2024, Volume and Issue: 16(3), P. 478 - 478
Published: March 20, 2024
Healthcare faces a major problem with the increased emergence of antimicrobial resistance due to over-prescribing antibiotics. Bacteriophages may provide solution treatment bacterial infections given their specificity. Enzymes such as endolysins, exolysins, endopeptidases, endosialidases, and depolymerases produced by phages interact surfaces, cell wall components, exopolysaccharides, even destroy biofilms. Enzymatic cleavage host envelope components exposes specific receptors required for phage adhesion. Gram-positive bacteria are susceptible infiltration through peptidoglycan, teichoic acid (WTA), lipoteichoic acids (LTAs), flagella. In Gram-negative bacteria, lipopolysaccharides (LPSs), pili, capsules serve targets. Defense mechanisms used differ include physical barriers (e.g., capsules) or endogenous clustered regularly interspaced palindromic repeat (CRISPR)-associated protein (Cas) systems. Phage proteins stimulate immune responses against pathogens improve antibiotic susceptibility. This review discusses attachment cells, penetration use in infections, limitations therapy. The therapeutic potential phage-derived impact that genomically engineered have summarized.
Language: Английский
Citations
24
Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)
Published: March 12, 2024
Abstract Bacteriophages constitute an invaluable biological reservoir for biotechnology and medicine. The ability to exploit such vast resources is hampered by the lack of methods rapidly engineer, assemble, package genomes, select phages. Cell-free transcription-translation (TXTL) offers experimental settings address a limitation. Here, we describe PHage Engineering In vitro Gene Expression Selection (PHEIGES) using T7 phage genome Escherichia coli TXTL. Phage genomes are assembled in from PCR-amplified fragments directly expressed batch TXTL reactions produce up 10 11 PFU/ml engineered phages within one day. We further demonstrate significant genotype-phenotype linkage assembly bulk This enables rapid selection with altered rough lipopolysaccharides specificity incorporating tail fiber mutant libraries. establish scalability PHEIGES pot mutants fluorescent gene integration 10% length-reduced genome.
Language: Английский
Citations
22
Nature Reviews Methods Primers, Journal Year: 2025, Volume and Issue: 5(1)
Published: Feb. 13, 2025
Citations
2