Pressure injuries and biofilms: Microbiome, model systems and therapies DOI Creative Commons

Fahad Kabir,

Deborah Bow Yue Yung, Waleska Stephanie da Cruz Nizer

et al.

Wound Repair and Regeneration, Journal Year: 2025, Volume and Issue: 33(1)

Published: Jan. 1, 2025

Abstract Chronic wounds have emerged as significant clinical problems owing to their increasing incidence and greater recognition of associated morbidity socio‐economic burden. They are defined that do not progress normally through the stages healing in a timely and/or orderly manner. Pressure injuries, particular, represent serious problem for patients who elderly or limited mobility, such wheelchair users those spend most day bed. These injuries often result from prolonged pressure exerted on skin over bone. Treatment is complex costly. Emerging evidence suggests injury microbiome plays vital role chronic wound formation delaying healing. Additionally, antibiotics fail due resistant biofilms emergence antimicrobial‐resistant bacteria. In this review, we will summarise current knowledge on: (a) microbiomes injuries; (b) vitro vivo model systems study (c) therapies novel treatment approaches. Understanding interactions between microbes host immune system provide valuable insights improve patient outcomes.

Language: Английский

Alternatives Therapeutic Approaches to Conventional Antibiotics: Advantages, Limitations and Potential Application in Medicine DOI Creative Commons

Hiba Alaoui Mdarhri,

Rachid Benmessaoud,

Houda Yacoubi

et al.

Antibiotics, Journal Year: 2022, Volume and Issue: 11(12), P. 1826 - 1826

Published: Dec. 16, 2022

Resistance to antimicrobials and particularly multidrug resistance is one of the greatest challenges in health system nowadays. The continual increase rates antimicrobial worldwide boosted by ongoing COVID-19 pandemic poses a major public threat. Different approaches have been employed minimize effect control this threat, but question still lingers as their safety efficiency. In context, new anti-infectious against are being examined. Use antibiotics combination with β-lactamase inhibitors, phage therapy, peptides, nanoparticles, antisense therapeutics considered such promising approach for overcoming bacterial resistance. review, we provide insights into these emerging alternative therapies that currently evaluated which may be developed future break progression We focus on advantages limitations potential application medicine. further highlight importance therapy approach, wherein two or more used order effectively combat infectious disease increasing access quality healthcare. These advances could give an alternate solution overcome drug eventually hope useful information clinicians who seeking solutions problems caused

Language: Английский

Citations

72
Biofilm-mediated infections by multidrug-resistant microbes: a comprehensive exploration and forward perspectives DOI Creative Commons
Mai M. Zafer, Gamal A. Mohamed, Sabrin R. M. Ibrahim

et al.

Archives of Microbiology, Journal Year: 2024, Volume and Issue: 206(3)

Published: Feb. 14, 2024

Abstract A biofilm is a collection of microorganisms organized in matrix extracellular polymeric material. Biofilms consist microbial cells that attach to both surfaces and each other, whether they are living or non-living. These biofilms can lead hospital-acquired infections generally detrimental. They possess the ability resist human immune system antibiotics. The National Institute Health (NIH) states formation associated with 65% all illnesses 80% chronic illnesses. Additionally, non-device-related include conditions like cystic fibrosis, otitis media, infective endocarditis, inflammatory disorders. This review aims provide an overview research on caused by biofilms, methods used for detection, recent approaches combat future perspectives, including development innovative antimicrobial strategies such as peptides, bacteriophages, agents disrupt biofilms.

Language: Английский

Citations

29
An overview of the use of bacteriophages in the poultry industry: Successes, challenges, and possibilities for overcoming breakdowns DOI Creative Commons
Amr Abd El‐Wahab, Shereen Basiouni, Hesham R. El‐Seedi

et al.

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: March 21, 2023

The primary contaminants in poultry are Salmonella enterica , Campylobacter j ejun i, Escherichia coli, and Staphylococcus aureus . Their pathogenicity together with the widespread of these bacteria, contributes to many economic losses poses a threat public health. With increasing prevalence bacterial pathogens being resistant most conventional antibiotics, scientists have rekindled interest using bacteriophages as antimicrobial agents. Bacteriophage treatments also been investigated an alternative antibiotics industry. Bacteriophages’ high specificity may allow them only target specific pathogen infected animal. However, tailor-made sophisticated cocktail different could broaden their antibacterial activity typical situations multiple clinical strains infections. Bacteriophages not be used terms reducing contamination animals but also, under industrial conditions, they can safe disinfectants reduce on food-contact surfaces or carcasses. Nevertheless, bacteriophage therapies developed sufficiently for use. Problems resistance, safety, specificity, long-term stability must addressed particular. This review highlights benefits, challenges, current limitations applications

Language: Английский

Citations

28
Bacteriophage DNA induces an interrupted immune response during phage therapy in a chicken model DOI Creative Commons
Magdalena Podlacha, Lidia Gaffke, Łukasz Grabowski

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: March 13, 2024

One of the hopes for overcoming antibiotic resistance crisis is use bacteriophages to combat bacterial infections, so-called phage therapy. This therapeutic approach generally believed be safe humans and animals as phages should infect only prokaryotic cells. Nevertheless, recent studies suggested that might recognized by eukaryotic cells, inducing specific cellular responses. Here we show in chickens infected with Salmonella enterica treated a cocktail, are initially animal cells viruses, however, cGAS-STING pathway (one two major pathways innate antiviral response) blocked at stage IRF3 transcription factor phosphorylation. inhibition due inability RNA polymerase III recognize DNA produce dsRNA molecules which necessary stimulate large protein complex indispensable phosphorylation, indicating mechanism response impairment.

Language: Английский

Citations

10
Phage therapy: an alternative treatment modality for MDR bacterial infections DOI

Namrata Pal,

Poonam Sharma, Manoj Kumawat

et al.

Infectious Diseases, Journal Year: 2024, Volume and Issue: 56(10), P. 785 - 817

Published: July 17, 2024

The increasing global incidence of multidrug-resistant (MDR) bacterial infections threatens public health and compromises various aspects modern medicine. Recognising the urgency this issue, World Health Organisation has prioritised development novel antimicrobials to combat ESKAPEE pathogens. Comprising

Language: Английский

Citations

10
Toward Clinical Applications: Transforming Nonantibiotic Antibacterials into Effective Next-Generation Supramolecular Therapeutics DOI
Christian K. O. Dzuvor

ACS Nano, Journal Year: 2024, Volume and Issue: 18(4), P. 2564 - 2577

Published: Jan. 16, 2024

Antibiotic resistance is a major driver of morbidity and mortality worldwide, necessitating alternatives. Due to their mechanism action, bacteriophages, endolysins, antimicrobial peptides (coined herein as nonantibiotic antibacterials, NAA) have risen tackle this problem led paradigms in treating antibiotic-resistant bacterial infections. However, clinical applications remain challenging been seriously hampered by cytotoxicity, instability, weak bioactivity, low on-target bioavailability, high pro-inflammatory responses, shorter half-life, circulatory properties. Hence, transit preclinical phases beyond, it has become imperative radically engineer these alternatives into innovative revolutionary therapeutics overcome recalcitrant This perspective highlights the promise agents, limitations, promising designs, nanotechnology, delivery approaches that can be harnessed transform agents. Finally, I provide an outlook on remaining challenges need tackled for widespread administration.

Language: Английский

Citations

9
Phage Therapy: A Targeted Approach to Overcoming Antibiotic Resistance DOI Creative Commons
David B. Olawade, Oluwaseun Fapohunda,

Eghosasere Egbon

et al.

Microbial Pathogenesis, Journal Year: 2024, Volume and Issue: unknown, P. 107088 - 107088

Published: Oct. 1, 2024

Language: Английский

Citations

9
The applications of animal models in phage therapy: An update DOI Creative Commons
Fazal Mehmood Khan, Prasanth Manohar, Vijay Singh Gondil

et al.

Human Vaccines & Immunotherapeutics, Journal Year: 2023, Volume and Issue: 19(1)

Published: Jan. 2, 2023

The rapid increase in antibiotic resistance presents a dire situation necessitating the need for alternative therapeutic agents. Among current therapies, phage therapy (PT) is promising. This review extensively summarizes preclinical PT approaches various in-vivo models. has been evaluated several recent clinical trials. However, there are still unanswered concerns due to lack of appropriate regulation and pharmacokinetic data regarding application phages human procedures. In this review, we also presented state considered how animal models can be used adapt these therapies humans. development realistic solutions circumvent constraints critical advancing technology.

Language: Английский

Citations

18
Comprehensive strategies for controlling Listeria monocytogenes biofilms on food‐contact surfaces DOI Creative Commons

Zi Hua,

Meijun Zhu

Comprehensive Reviews in Food Science and Food Safety, Journal Year: 2024, Volume and Issue: 23(3)

Published: May 1, 2024

Abstract Listeria monocytogenes biofilms formed on food‐contact surfaces within food‐processing facilities pose a significant challenge, serving as persistent sources of cross‐contamination. In this review, we examined documented cases foodborne outbreaks and recalls linked to L. contamination equipment in the food production environment, provided an overview prevalence persistence different facilities, discussed environmental factors influencing its biofilm formation. We further delved into antimicrobial interventions, such chemical sanitizers, thermal treatments, biological control, physical treatment, other approaches for controlling surfaces. This review provides valuable insights challenge processing, offering foundation future research practical strategies enhance safety.

Language: Английский

Citations

8
Potential of an Isolated Bacteriophage to Inactivate Klebsiella pneumoniae: Preliminary Studies to Control Urinary Tract Infections DOI Creative Commons
João Duarte,

Carolina Máximo,

Pedro Costa

et al.

Antibiotics, Journal Year: 2024, Volume and Issue: 13(2), P. 195 - 195

Published: Feb. 19, 2024

Urinary tract infections (UTIs) caused by resistant Klebsiella pneumoniae can lead to severe clinical complications and even death. An alternative treatment option for infected patients is using bacteriophages. In the present study, we isolated phage VB_KPM_KP1LMA (KP1LMA) from sewage water a K. strain as host. Whole-genome analysis indicated that genome was double-stranded linear 176,096-bp long DNA molecule with 41.8% GC content did not contain virulence or antibiotic resistance genes. The inactivation potential of KP1LMA assessed in broth at an MOI 1 10, maximum 4.9 5.4 log CFU/mL, respectively, observed after 9 h. efficacy 10 also urine evaluate phage’s performance acidic environment. A 3.8 CFU/mL results suggest could potentially control UTI this pneumoniae, indicating same procedure be used UTIs other strains if new specific phages are isolated. Although has narrow host range, future, efforts made expand its spectrum activity combine others, enabling use against involved UTIs.

Language: Английский

Citations

7