Roles of gut microbiota in androgenetic alopecia: insights from Mendelian randomization analysis DOI Creative Commons

Haijing Fu,

Tianyi Xu,

Wumei Zhao

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: April 2, 2024

Background Androgenetic alopecia (AGA) is the most common type of androgen-associated hair loss. Previous studies have indicated an association between gut microbiota and AGA. To delve deeper, we executed a two-sample Mendelian randomization (MR) analysis to investigate potential causal relationship Methods A MR investigation was utilized into intricate interplay Information regarding 211 microbial taxa sourced from MiBioGen consortium. The summary statistics genome-wide (GWAS) for AGA were obtained FinnGen biobank, which included 195 cases 201,019 controls. Various analytical approaches, including Inverse Variance Weighting (IVW), Weighted Median, MR-Egger, Mode, Simple Mode employed evaluate impact on Sensitivity analyses subsequently conducted affirm robustness findings. Results unveiled genus Olsenella , Ruminococcaceae UCG-004 UCG-010 identified as risk factors associated with In contrast, family Acidaminococcaceae Anaerofilum along Ruminiclostridium 9, demonstrated protective effect. sensitivity provided additional assurance that findings current study less susceptible influence confounding variables biases. Conclusion has established link specific AGA, offering evidence identification more precisely targeted probiotics. This discovery aid in prevention, control, reversal progression.

Language: Английский

Extracellular vesicles as tools and targets in therapy for diseases DOI Creative Commons

Mudasir A. Kumar,

Sadaf Khursheed Baba,

Hana Q. Sadida

et al.

Signal Transduction and Targeted Therapy, Journal Year: 2024, Volume and Issue: 9(1)

Published: Feb. 5, 2024

Abstract Extracellular vesicles (EVs) are nano-sized, membranous structures secreted into the extracellular space. They exhibit diverse sizes, contents, and surface markers ubiquitously released from cells under normal pathological conditions. Human serum is a rich source of these EVs, though their isolation proteins non-EV lipid particles poses challenges. These transport various cellular components such as proteins, mRNAs, miRNAs, DNA, lipids across distances, influencing numerous physiological events, including those within tumor microenvironment (TME). Their pivotal roles in communication make EVs promising candidates for therapeutic agents, drug delivery systems, disease biomarkers. Especially cancer diagnostics, EV detection can pave way early identification offers potential diagnostic Moreover, subtypes emerging targeted tools, highlighting clinical significance. The need non-invasive biomarkers to monitor biological processes purposes remains unfulfilled. Tapping unique composition could unlock advanced avenues future. In this review, we discuss detail conditions, cancers (encompassing head neck, lung, gastric, breast, hepatocellular carcinoma), neurodegenerative disorders, diabetes, viral infections, autoimmune renal diseases, emphasizing advancements molecular diagnostics delivery.

Language: Английский

Citations

291
exoRBase 2.0: an atlas of mRNA, lncRNA and circRNA in extracellular vesicles from human biofluids DOI Creative Commons
Hongyan Lai, Yuchen Li,

Hena Zhang

et al.

Nucleic Acids Research, Journal Year: 2021, Volume and Issue: 50(D1), P. D118 - D128

Published: Oct. 21, 2021

Extracellular vesicles (EVs) are small membranous that contain an abundant cargo of different RNA species with specialized functions and clinical implications. Here, we introduce updated online database (http://www.exoRBase.org), exoRBase 2.0, which is a repository EV long RNAs (termed exLRs) derived from RNA-seq data analyses diverse human body fluids. In the number exLRs has increased to 19 643 messenger (mRNAs), 15 645 non-coding (lncRNAs) 79 084 circular (circRNAs) obtained ∼1000 blood, urine, cerebrospinal fluid (CSF) bile samples. Importantly, 2.0 not only integrates compares exLR expression profiles but also visualizes pathway-level functional changes heterogeneity origins circulating EVs in context physiological pathological conditions. Our provides attractive platform for identification novel signatures biofluids will aid discovery new biomarkers improve disease diagnosis therapy.

Language: Английский

Citations

148
Extracellular Vesicles in Blood: Sources, Effects, and Applications DOI Open Access
Ainhoa Alberro, Leire Iparraguirre, Adelaide Fernandes

et al.

International Journal of Molecular Sciences, Journal Year: 2021, Volume and Issue: 22(15), P. 8163 - 8163

Published: July 29, 2021

Extracellular vesicles (EVs) are important players for intercellular communication. EVs secreted by almost all cell types; they can transfer information between nearby or distant cells, and highly abundant in body fluids. In this review, we describe the general characteristics of EVs, as well isolation characterization approaches. Then, focus on one most relevant sources EVs: blood. Indeed, apart from blood diverse origins travel bloodstream. We present numerous types that have been found circulation. Besides, implications blood-derived both physiological pathological processes summarized, highlighting their potential biomarkers diagnosis, treatment monitoring, prognosis several diseases, also indicators modifications. Finally, applications introduced circulatory system discussed. use distinct origins, naturally produced engineered, autologous, allogeneic, even different species effects when Therefore, work provides a comprehensive overview components, effects,

Language: Английский

Citations

122
Extracellular vesicle analysis DOI
An Hendrix, Lien Lippens, Cláudio Pinheiro

et al.

Nature Reviews Methods Primers, Journal Year: 2023, Volume and Issue: 3(1)

Published: July 27, 2023

Language: Английский

Citations

81
Entry and exit of extracellular vesicles to and from the blood circulation DOI
Dalila Iannotta,

A Amruta,

Amanda W. Kijas

et al.

Nature Nanotechnology, Journal Year: 2023, Volume and Issue: 19(1), P. 13 - 20

Published: Dec. 18, 2023

Language: Английский

Citations

46
A beginner's guide to study extracellular vesicles in human blood plasma and serum DOI Creative Commons
Rienk Nieuwland, Pia Siljander

Journal of Extracellular Vesicles, Journal Year: 2024, Volume and Issue: 13(1)

Published: Jan. 1, 2024

Abstract Blood is the most commonly used body fluid for obtaining and studying extracellular vesicles (EVs). While blood a standard choice clinical analysis, using as source of EVs introduces multiple layers complexity. At Extracellular Vesicle Workshop organized by International Society Vesicles in Helsinki (2022), it became evident that beginner researchers lack trustworthy information on how to initiate their research avoid common pitfalls. This educational guide explains composition frequently terminology blood, provides guidelines collection, preparation plasma serum. It also basic principles isolating detecting while considering blood‐related factors. The goal this assist beginners offering concise evidence‐based introduction current knowledge available resources study EVs.

Language: Английский

Citations

39
Extracellular Vesicle Preparation and Analysis: A State‐of‐the‐Art Review DOI Creative Commons
Zesheng Wang, Xiaoyu Zhou, Qinglong Kong

et al.

Advanced Science, Journal Year: 2024, Volume and Issue: 11(30)

Published: June 14, 2024

Abstract In recent decades, research on Extracellular Vesicles (EVs) has gained prominence in the life sciences due to their critical roles both health and disease states, offering promising applications diagnosis, drug delivery, therapy. However, inherent heterogeneity complex origins pose significant challenges preparation, analysis, subsequent clinical application. This review is structured provide an overview of biogenesis, composition, various sources EVs, thereby laying groundwork for a detailed discussion contemporary techniques preparation analysis. Particular focus given state‐of‐the‐art technologies that employ microfluidic non‐microfluidic platforms EV processing. Furthermore, this discourse extends into innovative approaches incorporate artificial intelligence cutting‐edge electrochemical sensors, with particular emphasis single proposes current outlines prospective avenues future research. The objective motivate researchers innovate expand methods analysis fully unlocking biomedical potential.

Language: Английский

Citations

35
Circulating RNAs in prostate cancer patients DOI Creative Commons
Vera Mugoni, Yari Ciani, Caterina Nardella

et al.

Cancer Letters, Journal Year: 2021, Volume and Issue: 524, P. 57 - 69

Published: Oct. 15, 2021

Growing bodies of evidence have demonstrated that the identification prostate cancer (PCa) biomarkers in patients' blood and urine may remarkably improve PCa diagnosis progression monitoring. Among diverse cancer-derived circulating materials, extracellular RNA molecules (exRNAs) represent a compelling component to investigate cancer-related alterations. Once outside intracellular environment, exRNAs circulate biofluids either association with protein complexes or encapsulated inside vesicles (EVs). Notably, EV-associated RNAs (EV-RNAs) were used for development several assays (such as FDA-approved Progensa Prostate Cancer Antigen 3 (PCA3 test) aiming at improving early detection. EV-RNAs encompass mixture species, including small non-coding (e.g. miRNA circRNA), lncRNAs mRNAs. Several methods been proposed isolate EVs relevant RNAs, perform RNA-Seq studies identify potential biomarkers. However, circulation patient include multitude populations are released by both normal cells from different tissues, thereby leading heterogeneous EV-RNA-associated transcriptional signal. Decrypting complexity such composite signal is nowadays major challenge faced specific tumor-associated RNAs. Multiple deconvolution algorithms so far infer enrichment cancer-specific signals gene expression data. novel strategies sorting sequencing associated single will facilitate molecules. Altogether, summarized here demonstrate high using EV-RNA highlight urgent need technologies computational approaches characterize their cargo.

Language: Английский

Citations

67
An estimate of extracellular vesicle secretion rates of human blood cells DOI Creative Commons
Martin Auber, Per Svenningsen

Journal of Extracellular Biology, Journal Year: 2022, Volume and Issue: 1(6)

Published: June 1, 2022

Abstract Extracellular vesicles (EVs) have been implicated in the intercellular transfer of RNA and proteins through cellular secretion into extracellular space. In blood plasma, circulating EVs are mainly derived from cells; however, factors that control plasma EV abundance largely unknown. Here, we estimate rates for cell types using reported values cell‐specific abundances their parental cell's ubiquity healthy humans. While found contains on average ∼2 EVs/cell, EV‐to‐cell ratio spanned four orders magnitude 0.13 ± 0.1 erythrocyte‐derived EVs/erythrocyte to (1.9 1.3) × 10 3 monocyte‐derived EVs/monocyte. The steady‐state level was maintained by an estimated rate (1.5 0.4) 12 EVs/min. estimates were highest monocytes (45 21 EVs/cell/min) lowest erythrocytes ((3.2 3.0) −3 EVs/cell/min). basal not significantly correlated lifespan or size; observed a highly significant correlation mitochondrial enzyme activities. Together, our analysis indicates metabolism, example, via reactive oxygen species, affects increased rates, results provide resource understanding function human health disease.

Language: Английский

Citations

48
Circulating tumour cells and PD-L1-positive small extracellular vesicles: the liquid biopsy combination for prognostic information in patients with metastatic non-small cell lung cancer DOI Creative Commons
Zahra Eslami‐S, Luis Enrique Cortés‐Hernández,

Léa Sinoquet

et al.

British Journal of Cancer, Journal Year: 2023, Volume and Issue: 130(1), P. 63 - 72

Published: Nov. 16, 2023

Abstract Background Circulating tumour cells (CTCs), circulating DNA (ctDNA), and extracellular vesicles (EVs) are minimally invasive liquid biopsy biomarkers. This study investigated whether they predict prognosis, alone or in combination, heterogenous unbiased non-small cell lung cancer (NSCLC) patients. Methods Plasma samples of 54 advanced NSCLC patients from a prospective clinical trial. CtDNA mutations were identified using the UltraSEEK™ Lung Panel (MassARRAY® technology). PD-L1 expression was assessed small EVs (sEVs) an enzyme-linked immunosorbent assay. Results At least one ctDNA mutation detected 37% Mutations not correlated with overall survival (OS) (HR = 1.1, 95% CI 0.55; 1.83, P 0.980) progression-free (PFS) 1.00, 0.57–1.76, 0.991). High + sEV concentration OS 1.14, 1.03–1.26, 0.016), but PFS 1.08, 0.99–1.18, 0.095). The interaction analysis suggested that correlation changed function CTC presence/absence ( 0.036). combination highlighted worse prognosis for CTCs high 7.65, 3.11–18.83, < 0.001). mutational statuses tissue significantly 0.0001). Conclusion OS, mutations. Their combined may help to identify OS. Trial registration NCT02866149, Registered 01 June 2015, https://clinicaltrials.gov/ct2/show/study/NCT02866149 .

Language: Английский

Citations

25