The mosquito taste system and disease control

Proceedings of the National Academy of Sciences, Journal Year: 2020, Volume and Issue: 117(52), P. 32848 - 32856

Published: Dec. 23, 2020

Mosquitoes are a widely diverse group of organisms, comprising ∼3,500 species that live in an enormous range habitats. Some vectors diseases afflict hundreds millions people each year. Although understanding mosquito olfaction has progressed dramatically recent years, taste remains greatly understudied. Since is essential to feeding, egg laying, and mating decisions insects, improved mosquitoes could provide new mechanistic insight into many aspects their behavior. We guide current knowledge the field, we suggest wealth opportunities for research now enabled by scientific technological advances. also propose means which might be exploited strategies control, may urgently needed as geographical ranges vector increase with climate change.

Language: Английский

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Neurogenetic identification of mosquito sensory neurons

iScience, Journal Year: 2023, Volume and Issue: 26(5), P. 106690 - 106690

Published: April 19, 2023

Anopheles mosquitoes, as vectors for the malaria parasite, are a global threat to human health. To find and bite human, they utilize neurons within their sensory appendages. However, identity quantification of appendage lacking. Here we use neurogenetic approach label all in coluzzii mosquitoes. We homology assisted CRISPR knock-in (HACK) generate T2A-QF2w synaptic gene bruchpilot. membrane-targeted GFP reporter visualize brain quantify major chemosensory appendages (antenna, maxillary palp, labella, tarsi, ovipositor). By comparing labeling brp>GFP Orco>GFP predict extent expressing ionotropic receptors (IRs) or other receptors. This work introduces valuable genetic tool functional analysis mosquito neurobiology initiates characterization that guide behavior.

Language: Английский

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A spatial map of antennal-expressed ionotropic receptors in the malaria mosquito

Cell Reports, Journal Year: 2023, Volume and Issue: 42(2), P. 112101 - 112101

Published: Feb. 1, 2023

The mosquito's antenna represents its main olfactory appendage for detecting volatile chemical cues from the environment. Whole-mount fluorescence in situ hybridization of ionotropic receptors (IRs) expressed antennae reveals that might be divisible into proximal and distal functional domains. number IR-positive cells appear stereotyped within each antennal segment (flagellomere). Highly odor-tuning IRs exhibit distinct co-localization patterns with IR coreceptors Ir8a, Ir25a, Ir76b predict their properties. Genetic knockin vivo imaging IR41c-expressing neurons indicate both odor-induced activation inhibition response to select amine compounds. Targeted mutagenesis IR41c does not abolish behavioral responses Our study provides a comprehensive map IR-expressing mosquitoes. These findings show organizing principles Anopheles neurons, which underlie contribution detection behaviorally relevant odors.

Language: Английский

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Tick chemosensation and implications for novel control strategies

Current Opinion in Insect Science, Journal Year: 2024, Volume and Issue: 65, P. 101249 - 101249

Published: Aug. 6, 2024

Ticks pose a major threat to the health of humans and animals. The use synthetic acaricides repellents has raised concerns potential environmental risks increasing resistance in ticks. This article highlights importance research on tick chemosensation developing novel control agents. It provides review our current understanding chemosensory system proposes using receptor (CR) genes as molecular targets discover releases high-quality genomes provide unprecedented opportunities explore CR gene repertoires. Further functional characterization is necessary identify receptors for key chemical cues signals unravel whether involves ionotropic and/or metabotropic mechanisms.

Language: Английский

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Characterization of Chemosensory Responses on the Labellum of the Malaria Vector Mosquito, Anopheles coluzzii

Scientific Reports, Journal Year: 2018, Volume and Issue: 8(1)

Published: March 29, 2018

Abstract Anopheles gambiae coluzzii (An. coluzzii) uses olfaction to modulate a range of critical behaviors that are essential for survival and reproduction; most notably, host preference selection underlie its vectorial capacity human malaria. As is the case all mosquitoes, An. has three specialized peripheral olfactory appendages—the antennae, maxillary palps labella—which used detect orient in response large variety cues. Of these, neither molecular nor physiological significance labellum have been thoroughly characterized despite suggestions labial-derived odorant reception close-range attraction. Here we report global chemoreceptor transcriptome profiles together with systematic electrophysiological analysis labial T2 sensilla, associated behavioral responses female . Single sensillum recordings sensilla revealed robust odorants previously sweat oviposition sites identified 10-component blend elicited attraction dual-choice landing bioassay designed mimic seeking which non-blood fed females were significantly more attracted labial-responsive as compared gravid females. Taken together, these data suggest that, , derived from contribute host-seeking.

Language: Английский

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