Commonly Used Insect Repellents Hide Human Odors from Anopheles Mosquitoes

Current Biology, Journal Year: 2019, Volume and Issue: 29(21), P. 3669 - 3680.e5

Published: Oct. 17, 2019

Language: Английский

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Human attractive cues and mosquito host-seeking behavior

Trends in Parasitology, Journal Year: 2021, Volume and Issue: 38(3), P. 246 - 264

Published: Oct. 19, 2021

Language: Английский

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Molecular evolution of gustatory receptors in the Anopheles gambiae complex

BMC Ecology and Evolution, Journal Year: 2025, Volume and Issue: 25(1)

Published: March 17, 2025

Mosquitoes in the Anopheles (An.) gambiae species complex are major vectors of Plasmodium falciparum malaria. One reason for this is high anthropophily constituent An. coluzzii, sensu stricto, and arabiensis. In contrast, their sister quadriannulatus highly zoophilic. mosquitoes largely rely on chemical cues host-seeking, which primarily detected by four chemosensory gene families: olfactory receptors (Ors), ionotropic (Irs), gustatory (Grs), odorant binding proteins (Obps). Genes from these families that have been implicated host adaptation show evidence positive selection other insect species, including mosquitoes. As such, we analyzed molecular evolutionary patterns within complex, with a particular interest identifying Grs anthropophilic species. We identified sixteen potential using McDonald-Kreitman test, putative sugar two unknown ligands relatively expressed organs either coluzzii or quadriannulatus. addition, twelve purifying may experienced selective sweep DH three carbon dioxide receptor Gr24. also both coastal melas (West Africa) merus (East Africa). Our results, together transcriptomic data, identify as possible candidates involvement evolution vertebrate preference occurred farauti complex. They point to playing role recent some vast majority functions much still about more complete interpretation our data necessitates further characterization genes.

Language: Английский

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Mutagenesis of the orco odorant receptor co-receptor impairs olfactory function in the malaria vector Anopheles coluzzii

Insect Biochemistry and Molecular Biology, Journal Year: 2020, Volume and Issue: 127, P. 103497 - 103497

Published: Nov. 11, 2020

Language: Английский

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Olfactory systems across mosquito species

Cell and Tissue Research, Journal Year: 2021, Volume and Issue: 383(1), P. 75 - 90

Published: Jan. 1, 2021

There are 3559 species of mosquitoes in the world (Harbach 2018) but, so far, only a handful them have been focus olfactory neuroscience and neurobiology research. Here we discuss mosquito anatomy function connect these to ecology. We highlight least well-known thus most interesting aspects systems promising future directions. hope this review will encourage insect community work more broadly across instead focusing narrowly on main disease vectors.

Language: Английский

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Olfaction inAnophelesmosquitoes

Chemical Senses, Journal Year: 2021, Volume and Issue: 46

Published: Jan. 1, 2021

Abstract As vectors of disease, mosquitoes are a global threat to human health. The Anopheles mosquito is the deadliest species as insect vector malaria-causing parasite, which kills hundreds thousands every year. These reliant on their sense smell (olfaction) guide most behaviors, and better understanding olfaction identifies opportunities for reducing spread malaria. This review takes detailed look at olfaction. We explore range topics from chemosensory receptors, olfactory neurons, sensory appendages behaviors guided by (including host-seeking, foraging, oviposition, mating), management strategies that target identify many research areas remain be addressed.

Language: Английский

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Discrete roles of Ir76b ionotropic coreceptor impact olfaction, blood feeding, and mating in the malaria vector mosquito Anopheles coluzzii

Proceedings of the National Academy of Sciences, Journal Year: 2022, Volume and Issue: 119(23)

Published: June 1, 2022

Significance Chemosensory systems play crucial roles across mosquito life cycles, with underlying molecular receptors often forming functional complexes that require cognate coreceptors. To better understand chemosensory pathways in the malaria vector Anopheles coluzzii , gene editing were used to localize and functionally characterize ionotropic receptor coreceptor AcIr76b . Expression of was observed antennal grooved pegs, coeloconic sensilla, other accessory olfactory appendages. When inactivated, mutant mosquitoes displayed altered neuronal responses amines.. Beyond phenotypes, mutants display significantly impaired mating blood feeding capabilities. Our data reveal discrete pathways, suggesting as targets for design control strategies.

Language: Английский

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Exploiting the chemical ecology of mosquito oviposition behavior in mosquito surveillance and control: a review

Journal of Vector Ecology, Journal Year: 2020, Volume and Issue: 45(2), P. 155 - 179

Published: Nov. 18, 2020

Vector control is an important component of the interventions aimed at mosquito-borne disease control. Current and future mosquito strategies are likely to rely largely on understanding behavior vector, by exploiting biology behavior, while using cost-effective, carefully timed larvicidal high-impact, low-volume adulticidal applications. Here we review knowledge ecology oviposition with emphasis potential role infochemicals in surveillance diseases. A search PubMed, Embase, Web Science, Global Health Archive, Google Scholar databases was conducted keywords mosquito, infochemical, pheromone, kairomone, allomone, synomone, apneumone, attractant, host-seeking, oviposition. Articles English from 1974 2019 were reviewed gain comprehensive current resource-searching behavior. Oviposition many species mediated that comprise pheromones, kairomones, synomones, allomones, apneumones. The novel putative mediate subfamilies Anophelinae Culicinae identified. these other tribes discussed respect origin chemical cues how affect gravid mosquitoes. attractants deterrents can potentially be used for manipulation making protected resources unsuitable mosquitoes (push) luring them towards attractive sources (pull). In this review, targeting breeding sites environmentally friendly larvicides aim develop appropriate trap-and-kill techniques discussed.

Language: Английский

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Of Genes and Genomes: Mosquito Evolution and Diversity

Trends in Parasitology, Journal Year: 2018, Volume and Issue: 35(1), P. 32 - 51

Published: Nov. 1, 2018

Genomic sampling of mosquito diversity has greatly improved in recent years and looks set to take advantage emerging technologies explore even further. Evolutionary genomics analyses have unveiled dynamic patterns gene genome evolution likely linked adaptability that will guide future research control efforts. Functional assays helped characterise biological roles thousands genes, albeit with condition-patchy species-biased coverage is now starting be remedied. Comparative approaches are increasingly being applied contextualise enhance the interpretation results from multispecies studies an evolutionary perspective. These trends mean effective data sharing critical facilitate integrative meta-analyses fully harness benefits combined functional analyses. Mosquitoes widely despised for their exasperating buzzing irritating bites, more poignantly because, during blood-feeding, females may transmit pathogens cause devastating diseases. However, ability such viruses, filarial worms, or malaria parasites varies amongst ∼3500 recognised species. Applying omics sample this biology underlying these variations bringing greater resolution enhances our understanding evolution. Here we review current status omics, ‘mozomics’, resources advances applications evolution, a focus on intersection understand putative links between dynamism diversity. The application technologies, including genomics, transcriptomics, proteomics, characterisation molecular, cellular, organismal blossomed over last two decades. As vectors plethora human pathogens, mosquitoes been many pioneering efforts scale throughput new technologies. resulting nascent sequenced genomes represent logical framework building comprehensive knowledge bases support drive research. This then provides opportunities vast variety animal through assessments different genomic elements throughout life cycle. For example, transcriptomics proteomics determine which tissues, response what stimuli, genes expressed subsequently translated into proteins. understandably often focused key processes associated capacity thwarting Increasingly, investigations enhanced perspective combining population comparative advance both basic translational biodiversity. made possible by multiple species, but still represents only small fraction species strongly biased towards genus Anopheles. Current promise radically increase improve taxonomic balance, making it timely consider learnt so far where might lead. available transcriptomic major – mozomics they facilitated. We characterisations encoded repertoires, explorations protein-coding non-protein-coding genes. While currently rather species-imbalanced, already successfully informing initial sequencing, assembly, annotation first genome, primary African vector, Anopheles gambiae [1Holt R.A. et al.The sequence gambiae.Science. 2002; 298: 129-149Crossref PubMed Scopus (1391) Google Scholar], provided very opportunity use investigate insect biology. were necessity limited comparisons fruit fly, Drosophila melanogaster, as other at time. broad chromosomal-arm-level homology was evident, about half appeared maintained single-copy orthologues (see Glossary), extensive differences dipterans indicated much rapid divergence insects compared vertebrates [2Zdobnov E.M. al.Comparative proteome analysis melanogaster.Science. 149-159Crossref (0) Scholar]. pairwise laid foundations subsequent applying sequencing From [3Harbach R.E. Besansky N.J. Mosquitoes.Curr. Biol. 2014; 24: R14-R15Abstract Full Text PDF Culicinae vector yellow dengue fevers, Aedes aegypti [4Nene V. al.Genome aegypti, arbovirus vector.Science. 2007; 316: 1718-1723Crossref (676) West Nile Saint Louis encephalitis viruses Culex quinquefasciatus [5Arensburger P. al.Sequencing establishes platform genomics.Science. 2010; 330: 86-88Crossref (247) larger culicine revealed high levels turnover notable expanded repertoires zinc-finger pheromone-binding proteins, olfactory gustatory receptors, well involved immune responses resistance insecticides. across three genera therefore established baseline expectation conserved divergent common unique features Recent increased Anophelinae particular those most closely related An. (Figure 1, Key Figure). National Centre Biotechnology Information (NCBI) Genome database lists total 28 projects mosquitoes, mere 1.7% 1657 reported NCBI’s Taxonomy 0.8% 3556 Mosquito Taxonomic Inventoryi. published assemblies annotations hosted VectorBaseii (Table 1), Institute Allergy Infectious Diseases Bioinformatics Resource scientific community tools access analyse invertebrate [6Giraldo-Calderón G.I. al.VectorBase: updated bioinformatics resource organisms diseases.Nucleic Acids Res. 2015; 43: D707-D713Crossref Small though be, facilitated detailed dynamics offer insights changing environments coevolution Scholar, 5Arensburger 7Waterhouse R.M. al.Evolutionary immune-related pathways disease-vector mosquitoes.Science. 1738-1743Crossref (329) 8Bartholomay L.C. al.Pathogenomics meta-analysis infection diverse pathogens.Science. 88-90Crossref (85) 9Neafsey D.E. al.Highly evolvable vectors: 16 347 (1258522–1258522)Crossref (162) Scholar].Table 1Sequenced Genomes Available VectorBase (VB-2018-06)GenusSubgenusSpeciesNotesRefsAedesStegomyiaaegyptiLiverpool strain, chromosomal-levelassembly (Liverpool AGWG strain),and cell line Aag2 assemblyNene al. 2007 4Nene ScholarDudchenko 2017 28Dudchenko O. al.De novo assembly using Hi-C yields chromosome-length scaffolds.Science. 2017; 356: 92-95Crossref (42) ScholarMatthews 29Mattews B.J. al.Improved reference informs control.Nature. 2018; 563: 501-507https://doi.org/10.1038/s41586-018-0692-zCrossref (2) ScholarWhitfield 95Whitfield Z.J. diversity, structure, function heritable adaptive immunity sequences genome.Curr. 27: 3511-3519.e7Abstract ScholarStegomyiaalbopictusFoshan (Chinese) Rimini (Italian)strains, C6/36 assemblyChen 2015 40Chen X.-G. Asian tiger mosquito, albopictus, reveals its biology, genetics, evolution.Proc. Natl. Acad. Sci. U. S. A. 112: E5907-E5915Crossref ScholarDritsou 110Dritsou al.A draft invasive mosquito: Italian albopictus.Pathog. Glob. Health. 109: 207-220Crossref (12) ScholarMiller 2018 102Miller J.R. al.Analysis albopictus insight utility viral propagation.Gigascience. 7: 1-13Crossref ScholarCulexCulexquinquefasciatusJohannesburg strain,now chromosomal-level assemblyArensburger 2010 ScholarAnophelesNyssorhynchusalbimanusSTECLA strain (El Salvador),now assemblyNeafsey ScholarArtemov 99Artemov G.N. physical mapping albimanus corrected scaffold misassemblies identified interarm rearrangements Anopheles.G3. 155-164Crossref (6) ScholardarlingiCoari (Amazonas, Brazil)Marinotti 2013 10Marinotti darlingi, main neotropical vector.Nucleic 2013; 41: 7387-7400Crossref (48) ScholarAnophelesatroparvusEBRO (Spain)now 100Artemov al.Partial-arm translocations high-coverage atroparvus genome.BMC Genomics. 19: 278Crossref (1) ScholarsinensisChina SINENSIS (Korea) strainsZhou 2014 12Zhou D. sinensis genetics basis competence parasites.BMC 15: 42Crossref (30) ScholarNeafsey ScholarCellia (Neomyzomyia)aAnopheline Cellia grouped series.dirusWRAIR2 (Thailand)Neafsey ScholarfarautiFAR1 (Papua New Guinea)koliensisAKwgs3 Guinea),contig-level assemblyLogue 111Logue K. al.Whole-genome absence flow separate demographic histories punctulatus Papua Guinea.Mol. Ecol. 1263-1274Crossref (4) ScholarpunctulatusAPwgs2 assemblyniliDinderesso (Burkina Faso),contig-level assemblyPeery 2011 112Peery al.Improving toolbox study nili: microsatellite chromosomes.Parasit. Vectors. 2011; 4: 202Crossref (5) (Myzomyia)aAnopheline series.culicifaciesA-37 (Iran)Neafsey ScholarfunestusFUMOZ (Mozambique)minimusMINIMUS1 (Thailand)Cellia (Neocellia)aAnopheline series.maculatusmaculatus3 (Malaysia)stephensiIndian SDA-500 (Pakistan) strainsJiang 11Jiang X. urban stephensi.Genome 459Crossref (41) (Pyretophorus)aAnopheline series.coluzziiFormerly M (Mali-NIH) strainLawniczak 113Lawniczak M.K. al.Widespread incipient whole sequences.Science. 512-514Crossref (173) ScholargambiaePEST assemblyPimperena (Mali) strainHolt 2002 1Holt ScholarLawniczak ScholararabiensisDongola (Sudan) strainNeafsey ScholarchristyiACHKN1017 (Kenya) strainepiroticusEpiroticus2 (Vietnam) strainmelasCM1001059_A (Cameroon) strainmerusMAF (South Africa) strainquadriannulatusSANGWE straina Anopheline series. Open table tab Concerted develop representative [9Neafsey Scholar] nucleotide-level genus. span range distances gambiae, native Africa, Asia, Australasia, Europe, South Central America, exploit ecological conditions, display varying degrees vectorial capacity. Short-read libraries insert sizes laboratory colonies wild-caught specimens produced 170–290 megabasepair (Mbp) variable contiguity. Maintained VectorBase, enable analyses, individual families genomes, also benefit integration studies, discoveries. project exemplifies power 13Neafsey project.G3. 3: 1191-1194Crossref Characterising conservation phylogeny terms orthologous regions, synteny, showed level chromosomal arms synteny highly conserved, contrasted within-arm shuffling. Such frequent X chromosome than autosomes, trend observed flies [14Bhutkar al.Chromosomal rearrangement inferred 12 genomes.Genetics. 2008; 179: 1657-1680Crossref appears elevated mosquitoes. Where inter-arm observed, again stood out, revealing notably exodus autosomes. could effects dosage compensation, transcriptional suppression, repeat content [15Jiang al.Complete compensation stephensi sex-biased mosquitoes.Genome Evol. 1914-1924Crossref 16Rose G. al.Dosage gambiae.Genome 2016; 8: 411-425PubMed 17Cassone testes: function, linkage.G3. 1127-1136Crossref 18Papa F. al.Rapid female-biased among four 1536-1548Crossref (3) make inhospitable habitat some elusive Y chromosomes required tackle challenges repeat-rich heterochromatic regions [19Hall A.B. al.Radical remodeling radiation mosquitoes.Proc. 113: E2114-E2123Crossref superdynamic remodelling members complex, single found exclusively Y-linked all examined male-determining factor (M-factor) gene, called YG2 YOB, regulates male-specific splicing doublesex early embryos microinjection YOB transcripts resulted male-only broods silencing male-lethal [20Krzywinska E. maleness 353: 67-69Crossref (13) Despite striking differences, hybrids arabiensis background no substantial fitness reductions, indicating hybrid incompatibility unlikely due loci thus possible, rare [21Bernardini al.Cross-species complex.Genetics. 207: 729-740PubMed Interestingly, candidate M-factor stephensi, GUY1 [22Criscione al.GUY1 confers complete female lethality strong stephensi.eLife. 5e19281Crossref same length similar secondary structure shows clear homology. characterizations important male successful development chromosome-based strategies. Ongoing unlock secrets recalcitrant extend view complex In sex determination instead controlled dominant region M-locus otherwise homomorphic 1. hunt Ae. DNA myo-sex male-biased tightly [23Hall al.Insights preservation sex-determining discovery M-locus.Genome 6: 179-191Crossref Chromosome quotient reassembled Nix, homologous transformer-2 [24Hall aegypti.Science. 348: 1268-1270Crossref Nix persistently embryos, somatic knockout feminization males while ectopic expression led masculinizing effects. Genetic crossing-over events caused ratio distortions sex-specific lethal [25Krzywinska locus factors conferring aegypti.Heredity (Edinb). 117: 408-416Crossref suggested there several within neighbourhood lost gained recombination, causing either females. Although itself relatively small, sits sex-differentiated driven reduced recombination [26Fontaine al.Extensive genetic differentiation aegypti.Genome 9: 2322-2335Crossref Exome populations male/female type form [27Campbell C.L. al.Alternative (L).BMC 18: 943Crossref spread entire 1 Senegalese population, possibly proposed cryptic subspecies. lag release discoveries part fragmentation because missing. Extensive relying complementary approaches, contact map-based chromosome-level [28Dudchenko assembled m-locus [29Mattews 100 Kbp intron neighbouring sit repetitive ∼1.5 Mbp M-locus, localised 1p pericentromeric (1p11) ∼100 excess sex-differentiation. makes begin reliably assess conflicts prevented transformation heteromorphic chromosomes. Sex cannot monopolise attention when comes dynamism. Rather, autosomes dynamic: phylogenomic uncovered pervasive introgression interspecies exchanges [30Fontaine M.C. phylogenomics.Science. (1258524–1258524)Crossref (147) task resolving correct branching order extremely challenging, extent meant taking ‘majority rule’ approach alignable sites full bootstrap wrong order. contrast, relationships, consistent hypothesis disproportionately harbours responsible reproductive isolation (e.g., assortative mating [31Aboagye-Antwi al.Experimental swap gambiae’s preferences demonstrates role X-chromosome island sympatric speciation.PLoS Genet. 11e1005141Crossref Scholar]) largely resistant introgression. boundaries fluid, prompt rethink classic concept phylogenetic tree: case, network useful ‘rampant reticulate evolution’ [32Clark A.G. Messer P.W. Conundrum jumbled genomes.Science. 347: 27-28Crossref (9) Indeed, methods substantially refined inference extent, direction, distribution [33Wen al.Reticulate history analysis.Mol. 25: 2361-2372Crossref (25) Furthermore, alternative resequencing enabled detection introgressed sister [34Rosenzweig B.K. al.Powerful detecting data.Mol. 2387-2397Crossref (20) Disentangling sometimes conflicting signals refinement methodologies, example 34Rosenzweig 35Thawornwattana Y. al.Coalescent confidently resolves relationships complex.Mol. E

Language: Английский

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Neurogenetic identification of mosquito sensory neurons

iScience, Journal Year: 2023, Volume and Issue: 26(5), P. 106690 - 106690

Published: April 19, 2023

Anopheles mosquitoes, as vectors for the malaria parasite, are a global threat to human health. To find and bite human, they utilize neurons within their sensory appendages. However, identity quantification of appendage lacking. Here we use neurogenetic approach label all in coluzzii mosquitoes. We homology assisted CRISPR knock-in (HACK) generate T2A-QF2w synaptic gene bruchpilot. membrane-targeted GFP reporter visualize brain quantify major chemosensory appendages (antenna, maxillary palp, labella, tarsi, ovipositor). By comparing labeling brp>GFP Orco>GFP predict extent expressing ionotropic receptors (IRs) or other receptors. This work introduces valuable genetic tool functional analysis mosquito neurobiology initiates characterization that guide behavior.

Language: Английский

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