Oscillatory Population-Level Activity of Dorsal Raphe Serotonergic Neurons Is Inscribed in Sleep Structure DOI Open Access
Tomonobu Kato, Yasue Mitsukura, Keitaro Yoshida

et al.

Journal of Neuroscience, Journal Year: 2022, Volume and Issue: 42(38), P. 7244 - 7255

Published: Aug. 15, 2022

Dorsal raphe (DR) 5-HT neurons regulate sleep–wake transitions. Previous studies demonstrated that single-unit activity of DR is high during wakefulness, decreases non-rapid eye movement (NREM) sleep, and ceases rapid (REM) sleep. However, characteristics the population-level neurons, which influence entire brain, are largely unknown. Here, we measured population activities in male female mouse across cycle by ratiometric fiber photometry. We found a slow oscillatory compound intracellular Ca2+ signals NREM The trough concave increased sleep progression, but always returned to seen wake period. When reached minimum remained there, REM was initiated. also unique coupling wideband EEG power fluctuation. Furthermore, optogenetic activation triggered EMG induced demonstrating causal role neuron activation. Optogenetic inhibition or sustained NREM, with an increase fluctuation, pharmacological silencing using selective serotonin reuptake inhibitor led decrease These inhibitory manipulations supported association between propose not monotonous state, rather it contains dynamic changes coincide neurons. SIGNIFICANCE STATEMENT have single-cell neuronal conditions. these well understood. monitored photometry system mice highest wakefulness lowest Surprisingly, non-REM decreased pattern, coinciding fluctuations. fluctuations persisted when silenced either interventions suggesting two. Although did generate fluctuations, provides evidence exhibits at least binary states.

Language: Английский

Sleep microstructure organizes memory replay DOI Creative Commons
Hongyu Chang, Wenbo Tang,

Annabella M. Wulf

et al.

Nature, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

4
Activation of locus coeruleus noradrenergic neurons rapidly drives homeostatic sleep pressure DOI Creative Commons
Daniel Silverman, Changwan Chen, Shuang Chang

et al.

Science Advances, Journal Year: 2025, Volume and Issue: 11(3)

Published: Jan. 17, 2025

Homeostatic sleep regulation is essential for optimizing the amount and timing of its revitalizing function, but mechanism underlying homeostasis remains poorly understood. Here, we show that optogenetic activation locus coeruleus (LC) noradrenergic neurons immediately increased propensity following a transient wakefulness, contrasting with many other arousal-promoting whose induces sustained wakefulness. Fiber photometry showed repeated or sensory stimulation caused rapid reduction calcium activity in LC steep declines noradrenaline/norepinephrine (NE) release both medial prefrontal cortex (mPFC). Knockdown α 2 A adrenergic receptors mitigated decline NE induced by repetitive extended demonstrating an important role receptor–mediated auto-suppression release. Together, these results suggest functional fatigue neurons, which reduces their wake-promoting capacity, contributes to pressure.

Language: Английский

Citations

3
Neuro-orchestration of sleep and wakefulness DOI
Bibi A. Sulaman, Su Wang, Jean Tyan

et al.

Nature Neuroscience, Journal Year: 2022, Volume and Issue: 26(2), P. 196 - 212

Published: Dec. 29, 2022

Language: Английский

Citations

68
When the Locus Coeruleus Speaks Up in Sleep: Recent Insights, Emerging Perspectives DOI Open Access
Alejandro Osorio-Forero, Najma Cherrad, Lila Banterle

et al.

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(9), P. 5028 - 5028

Published: April 30, 2022

For decades, numerous seminal studies have built our understanding of the locus coeruleus (LC), vertebrate brain’s principal noradrenergic system. Containing a numerically small but broadly efferent cell population, LC provides brain-wide modulation that optimizes network function in context attentive and flexible interaction with sensory environment. This review turns attention to LC’s roles during sleep. We show these go beyond down-scaled versions ones wakefulness. Novel dynamic assessments noradrenaline signaling activity uncover rich diversity patterns establish as an integral portion sleep regulation function. The could be involved beneficial functions for sleeping brain, even minute alterations its functionality may prove quintessential disorders.

Language: Английский

Citations

61
A noradrenergic-hypothalamic neural substrate for stress-induced sleep disturbances DOI Creative Commons
Hanna Antila, Iris Kwak, Ashley Choi

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2022, Volume and Issue: 119(45)

Published: Nov. 4, 2022

In our daily life, we are exposed to uncontrollable and stressful events that disrupt sleep. However, the underlying neural mechanisms deteriorating quality of non-rapid eye movement sleep (NREMs) REM largely unknown. Here, show in mice acute psychosocial stress disrupts by increasing brief arousals (microarousals [MAs]), reducing spindles, impairing infraslow oscillations spindle band electroencephalogram during NREMs, while REMs. This poor was reflected an increased number calcium transients activity noradrenergic (NE) neurons locus coeruleus (LC) NREMs. Opto- chemogenetic LC-NE activation naïve is sufficient change microarchitecture similar stress. Conversely, chemogenetically inhibiting reduced MAs NREMs normalized their after Specifically projecting preoptic area hypothalamus (POA) decreased enhanced spindles REMs Optrode recordings revealed stimulating fibers POA indeed suppressed spiking activated inactivated MAs. Our findings reveal changes dynamics stress-regulatory negatively affect quality, partially through interaction with POA.

Language: Английский

Citations

50
REM sleep is associated with distinct global cortical dynamics and controlled by occipital cortex DOI Creative Commons
Ziyue Wang, Xiang Fei, Xiaotong Liu

et al.

Nature Communications, Journal Year: 2022, Volume and Issue: 13(1)

Published: Nov. 12, 2022

Abstract The cerebral cortex is spontaneously active during sleep, yet it unclear how this global cortical activity spatiotemporally organized, and whether such not only reflects sleep states but also contributes to state switching. Here we report that cortex-wide calcium imaging in mice revealed distinct stage-dependent spatiotemporal patterns of activity, modulation could regulate In particular, elevated activation the occipital regions (including retrosplenial visual areas) became dominant rapid-eye-movement (REM) sleep. Furthermore, pontogeniculooccipital (PGO) wave-like was associated with transitions REM optogenetic inhibition strongly promoted deep by suppressing NREM-to-REM transition. Thus, whereas subcortical networks are critical for initiating maintaining wakefulness states, plays an role controlling states.

Language: Английский

Citations

42
Noradrenergiclocus coeruleusactivity functionally partitions NREM sleep to gatekeep the NREM-REM sleep cycle DOI Creative Commons
Alejandro Osorio-Forero, Georgios Foustoukos, Romain Cardis

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: May 22, 2023

Abstract The noradrenergic locus coeruleus (LC) is vital for brain states underlying wakefulness, whereas its roles sleep remain uncertain. Combining mouse sleep-wake monitoring, behavioral manipulations, LC fiber photometry and closed-loop optogenetics, we found that neuronal activity partitioned non-rapid-eye-movement (NREMS) into alternating autonomic rule the NREMS-REMS cycle. High levels generated an autonomic-subcortical arousal state facilitated cortical microarousals, while low were obligatory REMS entries. Timed optogenetic inhibition revealed this functional alternation set duration of cycle by ruling entries during undisturbed when pressure was high. A stimulus-enriched, stress-promoting wakefulness increased high at expense ones in subsequent NREMS, fragmenting NREMS through microarousals delaying onset. We conclude fluctuations gatekeep NREM-REMS over recurrent infraslow intervals, but they also convey vulnerability to adverse wake experiences.

Language: Английский

Citations

33
Regulation of stress-induced sleep fragmentation by preoptic glutamatergic neurons DOI Creative Commons

Jennifer Smith,

Adam Honig-Frand, Hanna Antila

et al.

Current Biology, Journal Year: 2023, Volume and Issue: 34(1), P. 12 - 23.e5

Published: Dec. 13, 2023

Language: Английский

Citations

23
Infraslow noradrenergic locus coeruleus activity fluctuations are gatekeepers of the NREM–REM sleep cycle DOI
Alejandro Osorio-Forero, Georgios Foustoukos, Romain Cardis

et al.

Nature Neuroscience, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 25, 2024

Language: Английский

Citations

14
Shared EEG correlates between non-REM parasomnia experiences and dreams DOI Creative Commons

Jacinthe Cataldi,

Aurélie Stephan, José Haba‐Rubio

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: May 9, 2024

Sleepwalking and related parasomnias result from incomplete awakenings out of non-rapid eye movement sleep. Behavioral episodes can occur without consciousness or recollection, in relation to dream-like experiences. To understand what accounts for these differences recall, here we recorded parasomnia with high-density electroencephalography (EEG) interviewed participants immediately afterward about their Compared reports no experience (19%), conscious (56%) were preceded by high-amplitude EEG slow waves anterior cortical regions activation posterior regions, similar previously described correlates dreaming. Recall the content (56%), compared recall (25%), was associated higher right medial temporal region before onset. Our work suggests that experiences are those reported dreams may thus reflect core physiological processes involved sleep consciousness.

Language: Английский

Citations

11