High‐quality Fagopyrum esculentum genome provides insights into the flavonoid accumulation among different tissues and self‐incompatibility

Journal of Integrative Plant Biology, Journal Year: 2023, Volume and Issue: 65(6), P. 1423 - 1441

Published: Jan. 21, 2023

Common buckwheat (Fagopyrum esculentum) and Tartary tataricum), the two most widely cultivated species, differ greatly in flavonoid content reproductive mode. Here, we report first high-quality chromosome-level genome assembly of common with 1.2 Gb. Comparative genomic analysis revealed that underwent a burst long terminal repeat retrotransposons insertion accompanied by numerous large chromosome rearrangements after divergence from buckwheat. Moreover, multiple gene families involved stress tolerance biosynthesis such as multidrug toxic compound extrusion (MATE) chalcone synthase (CHS) significant expansion buckwheat, especially Integrated multi-omics identified high expression catechin biosynthesis-related genes flower seed rutin being important for differences type between these species. We also candidate key rutin-degrading enzyme (Ft8.2377) was highly expressed seed. In addition, haplotype-resolved locus containing many reportedly associated development pollen, which potentially related to self-incompatibility Our study provides resources facilitating future functional genomics-related research

Language: Английский

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Genome sequencing reveals the genetic architecture of heterostyly and domestication history of common buckwheat

Nature Plants, Journal Year: 2023, Volume and Issue: 9(8), P. 1236 - 1251

Published: Aug. 10, 2023

Language: Английский

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Convergent evolutionary patterns of heterostyly across angiosperms support the pollination-precision hypothesis

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Feb. 9, 2024

Since the insights by Charles Darwin, heterostyly, a floral polymorphism with morphs bearing stigmas and anthers at reciprocal heights, has become model system for study of natural selection. Based on his archetypal heterostylous flower, including regular symmetry, few stamens tube, Darwin hypothesised that heterostyly evolved to promote outcrossing through efficient pollen transfer between involving different areas pollinator's body, thus proposing seminal pollination-precision hypothesis. Here we update number other style-length polymorphic taxa 247 genera belonging 34 families, notably expanding known cases 20%. Using phylogenetic comparative analyses across angiosperms, show numerous independent origins associated actinomorphic, tubular flowers low sex organs, fused corolla, pollination long-tongued insects. These associations provide support Darwinian hypothesis as basis convergent evolution angiosperms.

Language: Английский

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Genomic analyses elucidate S‐locus evolution in response to intra‐specific losses of distyly in Primula vulgaris

Ecology and Evolution, Journal Year: 2024, Volume and Issue: 14(3)

Published: March 1, 2024

Distyly, a floral dimorphism that promotes outcrossing, is controlled by hemizygous genomic region known as the

Language: Английский

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Genomic evidence supports the genetic convergence of a supergene controlling the distylous floral syndrome

New Phytologist, Journal Year: 2022, Volume and Issue: 237(2), P. 601 - 614

Published: Oct. 14, 2022

Heterostyly, a plant sexual polymorphism controlled by the S-locus supergene, has evolved numerous times among angiosperm lineages and represents classic example of convergent evolution in form function. Determining whether underlying molecular convergence occurs could provide insights on constraints to floral evolution. Here, we investigated genes distylous Gelsemium (Gelsemiaceae) determine there is evidence with unrelated species. We used several approaches, including anatomical measurements sex-organ development transcriptome whole-genome sequencing, identify components supergene. also performed evolutionary analysis candidate compared them those reported Primula Turnera. The supergene contained four genes, which three appear have originated from gene duplication events within Gelsemiaceae. style-length GeCYP CYP734A50 likely arose same gene, CYP734A1. Three out elegans were hemizygous, as previously genomic genetic distyly distantly related help illuminate architecture involved heterostyly.

Language: Английский

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Haplotype‐resolved genome assembly provides insights into the evolution of S‐locus supergene in distylous Nymphoides indica

New Phytologist, Journal Year: 2023, Volume and Issue: 240(5), P. 2058 - 2071

Published: Sept. 17, 2023

Distyly has evolved independently in numerous animal-pollinated angiosperm lineages. Understanding of its molecular basis been restricted to a few species, primarily Primula. Here, we investigate the genetic architecture single diallelic locus (S-locus) supergene, linkage group functionally associated genes, and explore how it may have distylous Nymphoides indica, lineage flowering plants not previously investigated. We assembled haplotype-resolved genomes, used read-coverage-based genome-wide association study (rb-GWAS) locate S-locus co-expression network analysis gene networks underpinning development distyly, comparative genomic analyses origins supergene. identified three linked candidate genes - NinBAS1, NinKHZ2, NinS1 that were only evident short-styled morph hemizygous. Co-expression suggested brassinosteroids contribute dimorphic sex organs morph. Comparative indicated supergene likely via stepwise duplications affected by transposable element activities. Our provides novel insight into structure, regulation, evolution governing distyly N. indica. It also high-quality resources for future research on mechanisms underlying striking evolutionary convergence form function across heterostylous taxa.

Language: Английский

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PfPIN5 promotes style elongation by regulating cell length in Primula forbesii Franch

Annals of Botany, Journal Year: 2024, Volume and Issue: 133(3), P. 473 - 482

Published: Jan. 8, 2024

Abstract Background and Aims Style dimorphism is one of the polymorphic characteristics flowers in heterostylous plants, which have two types flowers: pin morph, with long styles shorter anthers, thrum short longer anthers. The formation dimorphic has received attention plant world. Previous studies showed that CYP734A50 Primula determined style length limited elongation brassinosteroid metabolic pathway was involved regulation length. However, it unknown whether there are other factors affecting Primula. Methods Differentially expressed genes highly morph were screened based on forbesii transcriptome data. Virus-induced gene silencing used to silence these genes, anatomical changes observed 20 days after injection. Key Results PfPIN5 styles. When silenced, shortened long-homostyle plants by shortening cells. Moreover, increased expression level significantly, increased. results indicated PfPIN5, an auxin efflux transporter gene, contributed P. forbesii. Conclusions implied might also be forbesii, providing a new for elucidating molecular mechanism

Language: Английский

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A hemizygous supergene controls homomorphic and heteromorphic self-incompatibility systems in Oleaceae

Current Biology, Journal Year: 2024, Volume and Issue: 34(9), P. 1977 - 1986.e8

Published: April 15, 2024

Language: Английский

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The homomorphic self-incompatibility system in Oleaceae is controlled by a hemizygous genomic region expressing a gibberellin pathway gene

Current Biology, Journal Year: 2024, Volume and Issue: 34(9), P. 1967 - 1976.e6

Published: April 15, 2024

Language: Английский

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An overview of single-cell high-throughput technology in plants

Elsevier eBooks, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 34

Published: Jan. 1, 2025

Language: Английский

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