bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown
Published: Dec. 12, 2023
Layer specific computations in the brain rely on neuronal processes establishing synaptic connections with partners distinct laminae. In
Language: Английский
Nature, Journal Year: 2024, Volume and Issue: 634(8032), P. 139 - 152
Published: Oct. 2, 2024
Language: Английский
Citations
87
Nature, Journal Year: 2024, Volume and Issue: 634(8032), P. 166 - 180
Published: Oct. 2, 2024
Language: Английский
Citations
23
Current Biology, Journal Year: 2024, Volume and Issue: 34(3), P. R102 - R116
Published: Feb. 1, 2024
Precise synaptic connectivity is a prerequisite for the function of neural circuits, yet individual neurons, taken out their developmental context, readily form unspecific synapses. How does genome encode brain wiring in light this apparent contradiction? Synaptic specificity outcome long series processes and mechanisms before, during after synapse formation. much promiscuity permissible or necessary at moment partner choice depends on extent to which prior development restricts available partners subsequent corrects initially made can thereby play important roles precise connectivity, but also facilitate flexibility robustness. In review, we assess experimental evidence prevalence promiscuous formation development. Many well-established approaches are based genetic perturbation an assessment only adult; make it difficult pinpoint when given defect mechanism occurred. many cases, such studies reveal that restrict availability already Subsequently, choice, factors including competency, interaction dynamics molecular recognition further partners. The discussion through lens suggests algorithmic process neurons capable continuously prevented from making wrong choices, with no single time point sufficient explain outcome.
Language: Английский
Citations
8
bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown
Published: Oct. 13, 2023
A catalog of neuronal cell types has often been called a "parts list" the brain, and regarded as prerequisite for understanding brain function. In optic lobe
Language: Английский
Citations
17
Cell Reports, Journal Year: 2024, Volume and Issue: 43(2), P. 113798 - 113798
Published: Feb. 1, 2024
Neurons establish specific synapses based on the adhesive properties of cell-surface proteins while also retaining ability to form in a relatively non-selective manner. However, comprehensive understanding underlying mechanism reconciling these opposing characteristics remains incomplete. Here, we have identified Side-IV/Beat-IIb, members Drosophila immunoglobulin superfamily, as combination recognition molecules inducing synapse formation. The Side-IV/Beat-IIb transduces bifurcated signaling with Side-IV's co-receptor, Kirre, and synaptic scaffold protein, Dsyd-1. Genetic experiments subcellular protein localization analyses showed Side-IV/Beat-IIb/Kirre/Dsyd-1 complex two essential functions. First, it narrows neuronal binding specificity through extracellular interactions. Second, recruits formation factors, Kirre Dsyd-1, restrict loci inhibit miswiring. This dual function explains how combinations enable ranking preferred interactions among pairs achieve circuits vivo.
Language: Английский
Citations
4
bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown
Published: March 4, 2025
Abstract Animal behaviors are executed by motor neurons (MNs), which receive information from complex pre-motor neuron (preMN) circuits and output commands to muscles. How established during development remains an important unsolved problem in neuroscience. Here we focus on the of that control movements adult legs Drosophila melanogaster . After generating single-cell RNA sequencing (scRNAseq) datasets for leg MNs at multiple time points, describe course gene expression families. This analysis reveals transcription factors (TFs) cell adhesion molecules (CAMs) appear drive molecular diversity between individual MNs. In parallel, introduce ConnectionMiner, a novel computational tool integrates scRNAseq data with electron microscopy-derived connectomes. ConnectionMiner probabilistically refines ambiguous type annotations leveraging neural wiring patterns, and, turn, it identifies combinatorial signatures correlate synaptic connectivity strength. Applied system, yields comprehensive transcriptional annotation both preMNs uncovers candidate effector combinations likely orchestrate assembly ultimately
Language: Английский
Citations
0
Published: April 3, 2025
Understanding the developmental changes in neuronal lineages is crucial to elucidate how they assemble into functional neural networks. Studies investigating nervous system development model systems have focused on only a few regions of central due limited availability genetic drivers that target specific throughout and adult life. This has hindered our understanding distinct interconnect form circuits during development. Here, we present split-GAL4 library composed driver lines, which generated via editing genomic locus lineage-specific transcription factors demonstrate can use this specifically most individual hemilineages Drosophila ventral nerve cord (VNC) adulthood. Using these found striking morphological processes within lineage metamorphosis. We also demonstrated neurochemical features classes be quickly assessed. Lastly, documented behaviors elicited response optogenetic activation comprehensive lineage-behavior map entire fly VNC. Looking forward, will provide tools needed address questions emerging from analysis recent VNC connectome transcriptome datasets.
Language: Английский
Citations
0
Published: April 3, 2025
Understanding the developmental changes in neuronal lineages is crucial to elucidate how they assemble into functional neural networks. Studies investigating nervous system development model systems have focused on only a few regions of central due limited availability genetic drivers that target specific throughout and adult life. This has hindered our understanding distinct interconnect form circuits during development. Here, we present split-GAL4 library composed driver lines, which generated via editing genomic locus lineage-specific transcription factors demonstrate can use this specifically most individual hemilineages Drosophila ventral nerve cord (VNC) adulthood. Using these found striking morphological processes within lineage metamorphosis. We also demonstrated neurochemical features classes be quickly assessed. Lastly, documented behaviors elicited response optogenetic activation comprehensive lineage-behavior map entire fly VNC. Looking forward, will provide tools needed address questions emerging from analysis recent VNC connectome transcriptome datasets.
Language: Английский
Citations
0
Published: April 22, 2024
Sensorimotor reflex circuits engage distinct neuronal subtypes, defined by precise connectivity, to transform sensation into compensatory behavior. Whether and how motor neuron populations specify the subtype fate and/or sensory connectivity of their pre-motor partners remains controversial. Here, we discovered that neurons are dispensable for proper in vestibular circuit stabilizes gaze. We first measured activity following premotor projection after constitutive loss extraocular partners. observed normal responses topography indicative unchanged functional between neurons. Next, show remain anatomically molecularly poised connect appropriately with downstream Lastly, transcriptional signatures typify develop independently Our findings comprehensively overturn a long-standing model: gaze stabilization is retrogradely determined partner-derived signals. By defining contribution specification an archetypal sensorimotor circuit, our work speaks comparable processes spinal cord advances understanding general principles neural development.
Language: Английский
Citations
2
eLife, Journal Year: 2024, Volume and Issue: 13
Published: April 22, 2024
Sensorimotor reflex circuits engage distinct neuronal subtypes, defined by precise connectivity, to transform sensation into compensatory behavior. Whether and how motor neuron populations specify the subtype fate and/or sensory connectivity of their pre-motor partners remains controversial. Here, we discovered that neurons are dispensable for proper in vestibular circuit stabilizes gaze. We first measured activity following projection after constitutive loss extraocular partners. observed normal responses topography indicative unchanged functional between neurons. Next, show remain anatomically molecularly poised connect appropriately with downstream Lastly, transcriptional signatures typify develop independently Our findings comprehensively overturn a long-standing model: gaze stabilization is retrogradely determined partner-derived signals. By defining contribution specification an archetypal sensorimotor circuit, our work speaks comparable processes spinal cord advances understanding principles neural development.
Language: Английский
Citations
2