Cooperative Control of Ecdysone Biosynthesis in Drosophila by Transcription Factors Séance, Ouija Board, and Molting Defective DOI Creative Commons

Outa Uryu,

Qiuxiang Ou,

Tatsuya Komura-Kawa

et al.

Genetics, Journal Year: 2017, Volume and Issue: 208(2), P. 605 - 622

Published: Nov. 30, 2017

Ecdysteroids are steroid hormones that control many aspects of development and physiology. During larval development, ecdysone is synthesized in an endocrine organ called the prothoracic gland through a series ecdysteroidogenic enzymes encoded by Halloween genes. The expression genes highly restricted dynamic, indicating their spatiotemporal regulation mediated tight transcriptional control. In this study, we report three zinc finger-associated domain (ZAD)-C2H2 finger transcription factors-Séance (Séan), Ouija board (Ouib), Molting defective (Mld)-cooperatively biosynthesis fruit fly Drosophila melanogaster Séan Ouib act cooperation with Mld to positively regulate neverland spookier, respectively, two Remarkably, loss-of-function mutations séan, ouib, or mld can be rescued neverland, both, respectively. These results suggest factors have distinct roles coordinating just Given spookier located constitutive heterochromatin, Séan, Ouib, represent first example factor subset regulates heterochromatin.

Language: Английский

Regulatory Pathways Controlling Female Insect Reproduction DOI Open Access
Sourav Roy,

Tusar T. Saha,

Zhen Zou

et al.

Annual Review of Entomology, Journal Year: 2017, Volume and Issue: 63(1), P. 489 - 511

Published: Oct. 23, 2017

The synthesis of vitellogenin and its uptake by maturing oocytes during egg maturation are essential for successful female reproduction. These events regulated the juvenile hormones ecdysteroids nutritional signaling pathway neuropeptides. Juvenile act as gonadotropins, regulating vitellogenesis in most insects, but control this process Diptera some Hymenoptera Lepidoptera. complex crosstalk between hormones, ecdysteroids, pathways differs distinctly depending on reproductive strategies adopted various insects. Molecular studies within past decade have revealed much about relationships among, role of, these with respect to regulation insect Here, we review signaling, along that microRNAs, reproduction at molecular level.

Language: Английский

Citations

443
Antagonistic actions of juvenile hormone and 20-hydroxyecdysone within the ring gland determine developmental transitions in Drosophila DOI Creative Commons
Suning Liu,

Kang Li,

Yue Gao

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2017, Volume and Issue: 115(1), P. 139 - 144

Published: Dec. 18, 2017

In both vertebrates and insects, developmental transition from the juvenile stage to adulthood is regulated by steroid hormones. hormone, 20-hydroxyecdysone (20E), elicits metamorphosis, thus promoting this transition, while sesquiterpenoid hormone (JH) antagonizes 20E signaling prevent precocious metamorphosis during larval stages. However, not much known about mechanisms involved in cross-talk between these two study, we discovered that ring gland (RG) of Drosophila larvae, JH control each other's biosynthesis. induces expression a Krüppel-like transcription factor gene Kr-h1 prothoracic (PG), portion RG produces precursor ecdysone. By reducing steroidogenesis autoregulation PG size, high levels inhibit ecdysteriod biosynthesis, maintaining status. biosynthesis prevented corpus allatum, other JH, ensure occurrence metamorphosis. Hence, antagonistic actions within determine transitions Our study proposes mechanism major hormones regulation insect

Language: Английский

Citations

175
Regulation of Body Size and Growth Control DOI Open Access
Michael J. Texada, Takashi Koyama, Kim Rewitz

et al.

Genetics, Journal Year: 2020, Volume and Issue: 216(2), P. 269 - 313

Published: Oct. 1, 2020

Abstract The control of body and organ growth is essential for the development adults with proper size proportions, which important survival reproduction. In animals, adult determined by rate duration juvenile growth, are influenced environment. nutrient-scarce environments in more time needed period can be extended delaying maturation, whereas rapidly completed nutrient-rich conditions. This flexibility requires integration environmental cues developmental signals that govern internal checkpoints to ensure maturation does not begin until sufficient tissue has occurred reach a size. Target Rapamycin (TOR) pathway primary cell-autonomous nutrient sensor, while circulating hormones such as steroids insulin-like factors main systemic regulators animals. We discuss recent findings Drosophila melanogaster showing environment growth-sensing mechanisms, involving TOR other growth-regulatory pathways, converge on insulin steroid relay centers responsible adjusting development, response external addition this, also monitored coordinated whole-body timing through modulation signaling. coordination involves interorgan communication mediated peptide 8 status. Together, these multiple nutritional feed into neuroendocrine hubs controlling signaling, serving at progression toward delayed. review focuses mechanisms conditions modulate size, highlights conserved architecture this system, made prime model understanding

Language: Английский

Citations

135
A Membrane Transporter Is Required for Steroid Hormone Uptake in Drosophila DOI Creative Commons
Naoki Okamoto, Raghuvir Viswanatha,

Riyan Bittar

et al.

Developmental Cell, Journal Year: 2018, Volume and Issue: 47(3), P. 294 - 305.e7

Published: Oct. 4, 2018

Language: Английский

Citations

124
Krüppel homolog 1 represses insect ecdysone biosynthesis by directly inhibiting the transcription of steroidogenic enzymes DOI Open Access
Tianlei Zhang, Wei Song, Zheng Li

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2018, Volume and Issue: 115(15), P. 3960 - 3965

Published: March 22, 2018

In insects, juvenile hormone (JH) and the steroid ecdysone have opposing effects on regulation of larval-pupal transition. Although increasing evidence suggests that JH represses biosynthesis during larval development, mechanism underlying this repression is not well understood. Here, we demonstrate expression Krüppel homolog 1 (Kr-h1), a gene encoding transcription factor mediates signaling, in ecdysone-producing organ prothoracic gland (PG) by directly inhibiting steroidogenic enzymes both Drosophila Bombyx Application mimic ex vivo cultured PGs from larvae induces Kr-h1 inhibits enzymes. addition, PG-specific knockdown promotes-while overexpression hampers-ecdysone production pupariation. We further find binding to their promoters induce promoter DNA methylation. Finally, show does affect replication PG cells reduction size mediated can be rescued feeding ecdysone. Taken together, our data indicate direct conserved insect response stimulation, providing insights into mechanisms antagonistic roles

Language: Английский

Citations

87
A versatile toolkit for CRISPR-Cas13-based RNA manipulation in Drosophila DOI Creative Commons
Nhan Huynh,

Noah Depner,

Raegan Larson

et al.

Genome biology, Journal Year: 2020, Volume and Issue: 21(1)

Published: Nov. 17, 2020

Abstract Advances in CRISPR technology have immensely improved our ability to manipulate nucleic acids, and the recent discovery of RNA-targeting endonuclease Cas13 adds even further functionality. Here, we show that works efficiently Drosophila , both ex vivo vivo. We test 44 different variants identify enzymes with best overall performance could target endogenous transcripts high efficiency specificity. also develop applications edit mRNAs mitochondrial transcripts. Our vector collection represents a versatile tool gene expression at post-transcriptional level.

Language: Английский

Citations

87
Control of the insect metamorphic transition by ecdysteroid production and secretion DOI
Xueyang Pan, Robert P. Connacher, Michael B. O’Connor

et al.

Current Opinion in Insect Science, Journal Year: 2020, Volume and Issue: 43, P. 11 - 20

Published: Sept. 17, 2020

Language: Английский

Citations

75
Emerging role of the Hippo pathway in autophagy DOI Creative Commons
Dongying Wang, Jiaxing He, Bingyu Huang

et al.

Cell Death and Disease, Journal Year: 2020, Volume and Issue: 11(10)

Published: Oct. 20, 2020

Abstract Autophagy is a dynamic circulatory system that occurs in all eukaryotic cells. Cytoplasmic material transported to lysosomes for degradation and recovery through autophagy. This provides energy macromolecular precursors cell renewal homeostasis. The Hippo-YAP pathway has significant biological properties controlling organ size, tissue homeostasis, regeneration. Recently, the axis been extensively referred as pathophysiological processes regulating Understanding cellular molecular basis of these crucial identifying disease pathogenesis novel therapeutic targets. Here we review recent findings from Drosophila models organisms. We particularly emphasize regulation between Hippo core components autophagy, which involved normal human diseases, its application treatment.

Language: Английский

Citations

72
Signaling Pathways That Regulate the Crustacean Molting Gland DOI Creative Commons
Donald L. Mykles

Frontiers in Endocrinology, Journal Year: 2021, Volume and Issue: 12

Published: June 21, 2021

A pair of Y-organs (YOs) are the molting glands decapod crustaceans. They synthesize and secrete steroid hormones (ecdysteroids) their activity is controlled by external internal signals. The YO transitions through four physiological states over molt cycle, which mediated molt-inhibiting hormone (MIH; basal state), mechanistic Target Rapamycin Complex 1 (mTORC1; activated Transforming Growth Factor-β (TGFβ)/Activin (committed ecdysteroid (repressed state) signaling pathways. MIH, produced in eyestalk X-organ/sinus gland complex, inhibits synthesis ecdysteroids. model for MIH organized into a cAMP/Ca

Language: Английский

Citations

59
Juvenile Hormone Studies in Drosophila melanogaster DOI Creative Commons
Xiaoshuai Zhang, Sheng Li, Suning Liu

et al.

Frontiers in Physiology, Journal Year: 2022, Volume and Issue: 12

Published: Feb. 10, 2022

In the field of insect endocrinology, juvenile hormone (JH) is one most wondrous entomological terms. As a unique sesquiterpenoid produced and released by endocrine gland, corpus allatum (CA), JH critical regulator in multiple developmental physiological processes, such as metamorphosis, reproduction, behavior. Benefited from precise genetic interventions simplicity, fruit fly,

Language: Английский

Citations

46