Drosophila Evi5 is a critical regulator of intracellular iron transport via transferrin and ferritin interactions

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: May 14, 2024

Abstract Vesicular transport is essential for delivering cargo to intracellular destinations. Evi5 a Rab11-GTPase-activating protein involved in endosome recycling. In humans, high-risk locus multiple sclerosis, debilitating disease that also presents with excess iron the CNS. insects, prothoracic gland (PG) requires entry of extracellular synthesize steroidogenic enzyme cofactors. The mechanism peripheral uptake insect cells remains controversial. We show Evi5-depletion Drosophila PG affected vesicle morphology and density, blocked recycling impaired trafficking transferrin-1, thus disrupting heme synthesis due reduced cellular concentrations. ferritin delivers as well, interacts physically Evi5. Further, ferritin-injection rescued developmental delays associated Evi5-depletion. To summarize, our findings critical via transferrin-1 ferritin, implicate altered homeostasis etiology sclerosis.

Language: Английский

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Insulin signaling couples growth and early maturation to cholesterol intake in Drosophila

Current Biology, Journal Year: 2022, Volume and Issue: 32(7), P. 1548 - 1562.e6

Published: March 3, 2022

Nutrition is one of the most important influences on growth and timing maturational transitions including mammalian puberty insect metamorphosis. Childhood obesity associated with precocious puberty, but assessment mechanism that links body fat to early maturation unknown. During development, intake nutrients promotes signaling through insulin-like systems govern cells tissues also regulates timely production steroid hormones initiate juvenile-adult transition. We show here dietary lipid cholesterol, which required as a component cell membranes substrate for biosynthesis, governs in Drosophila via promoting expression release peptides. This nutritional input acts nutrient sensor TOR, regulated by Niemann-Pick-type-C 1 (Npc1) cholesterol transporter, glia blood-brain barrier adipose tissue remotely drive systemic insulin growth. Furthermore, increasing intracellular levels steroid-producing prothoracic gland strongly endoreduplication, leading an accelerated attainment checkpoint normally ensures animals do not prematurely. These findings, therefore, Npc1-TOR system couples sensing cellular control timing, may help explain both link between cancer well connection (obesity) puberty.

Language: Английский

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Transcriptomic analysis of differentially expressed genes in the molting gland (Y-organ) of the blackback land crab, Gecarcinus lateralis, during molt-cycle stage transitions

Comparative Biochemistry and Physiology Part D Genomics and Proteomics, Journal Year: 2018, Volume and Issue: 28, P. 37 - 53

Published: June 15, 2018

Language: Английский

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RNAi-mediated knockdown of SPOOK reduces ecdysteroid titers and causes precocious metamorphosis in the desert locust Schistocerca gregaria

Developmental Biology, Journal Year: 2017, Volume and Issue: 429(1), P. 71 - 80

Published: July 19, 2017

Language: Английский

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Cooperative Control of Ecdysone Biosynthesis in Drosophila by Transcription Factors Séance, Ouija Board, and Molting Defective

Genetics, Journal Year: 2017, Volume and Issue: 208(2), P. 605 - 622

Published: Nov. 30, 2017

Ecdysteroids are steroid hormones that control many aspects of development and physiology. During larval development, ecdysone is synthesized in an endocrine organ called the prothoracic gland through a series ecdysteroidogenic enzymes encoded by Halloween genes. The expression genes highly restricted dynamic, indicating their spatiotemporal regulation mediated tight transcriptional control. In this study, we report three zinc finger-associated domain (ZAD)-C2H2 finger transcription factors-Séance (Séan), Ouija board (Ouib), Molting defective (Mld)-cooperatively biosynthesis fruit fly Drosophila melanogaster Séan Ouib act cooperation with Mld to positively regulate neverland spookier, respectively, two Remarkably, loss-of-function mutations séan, ouib, or mld can be rescued neverland, both, respectively. These results suggest factors have distinct roles coordinating just Given spookier located constitutive heterochromatin, Séan, Ouib, represent first example factor subset regulates heterochromatin.

Language: Английский

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