Biomolecules,
Journal Year:
2024,
Volume and Issue:
14(9), P. 1116 - 1116
Published: Sept. 4, 2024
Porcine
epidemic
diarrhea
virus
(PEDV)
has
caused
significant
economic
losses
to
the
pig
farming
industry
in
various
countries
for
a
long
time.
Currently,
there
are
no
highly
effective
preventive
or
control
measures
available.
Research
into
pathogenic
mechanism
of
PEDV
shown
that
it
primarily
causes
infection
by
binding
S
protein
CD13
(APN)
receptor
on
membrane
porcine
intestinal
epithelial
cells.
The
S1
region
contains
three
neutralization
epitopes
and
multiple
receptor-binding
domains,
which
closely
related
viral
antigenicity
ad-sorption
invasion.
Nanobodies
type
single-domain
antibody
have
been
discovered
recent
years.
They
can
be
expressed
large
scale
through
prokaryotic
expression
systems,
makes
them
cost-effective,
stable,
less
immunogenic.
This
study
used
phage
display
library
nanobodies
against
protein.
After
rounds
selection
enrichment,
DNA
sequence
specific
nanobody
S1Nb1
was
successfully
obtained.
To
obtain
soluble
S1Nb1,
its
inserted
vector
Pcold
solubility-enhancing
SUMO
tag
added.
resulting
recombinant
vector,
Pcold-SUMO-S1Nb1,
then
transformed
Cancers,
Journal Year:
2023,
Volume and Issue:
15(23), P. 5639 - 5639
Published: Nov. 29, 2023
Monoclonal
antibodies
(mAbs)
have
exhibited
substantial
potential
as
targeted
therapeutics
in
cancer
treatment
due
to
their
precise
antigen-binding
specificity.
Despite
success
tumor-targeted
therapies,
effectiveness
is
hindered
by
large
size
and
limited
tissue
permeability.
Camelid-derived
single-domain
antibodies,
also
known
nanobodies,
represent
the
smallest
naturally
occurring
antibody
fragments.
Nanobodies
offer
distinct
advantages
over
traditional
mAbs,
including
smaller
size,
high
stability,
lower
manufacturing
costs,
deeper
penetration
capabilities.
They
demonstrated
significant
roles
both
diagnostic
therapeutic
tools
research
are
considered
next
generation
of
drugs.
In
this
review,
our
objective
provide
readers
with
insights
into
development
various
applications
nanobodies
field
treatment,
along
an
exploration
challenges
strategies
for
prospective
clinical
trials.
Frontiers in Immunology,
Journal Year:
2024,
Volume and Issue:
15
Published: July 12, 2024
Currently,
immunotherapy
is
being
widely
used
for
treating
cancers.
However,
the
significant
heterogeneity
in
patient
responses
a
major
challenge
its
successful
application.
CD8-positive
T
cells
(CD8
+
cells)
play
critical
role
immunotherapy.
Both
their
infiltration
and
functional
status
tumors
contribute
to
treatment
outcomes.
Therefore,
accurate
monitoring
of
CD8
cells,
potential
biomarker,
may
improve
therapeutic
strategy.
Positron
emission
tomography
(PET)
an
optimal
option
which
can
provide
molecular
imaging
with
enhanced
specificity.
This
review
summarizes
mechanism
action
immunotherapy,
highlights
recent
advancements
PET-based
tracers
that
visualize
discusses
clinical
applications
elucidate
cancer
Biomolecules,
Journal Year:
2024,
Volume and Issue:
14(9), P. 1116 - 1116
Published: Sept. 4, 2024
Porcine
epidemic
diarrhea
virus
(PEDV)
has
caused
significant
economic
losses
to
the
pig
farming
industry
in
various
countries
for
a
long
time.
Currently,
there
are
no
highly
effective
preventive
or
control
measures
available.
Research
into
pathogenic
mechanism
of
PEDV
shown
that
it
primarily
causes
infection
by
binding
S
protein
CD13
(APN)
receptor
on
membrane
porcine
intestinal
epithelial
cells.
The
S1
region
contains
three
neutralization
epitopes
and
multiple
receptor-binding
domains,
which
closely
related
viral
antigenicity
ad-sorption
invasion.
Nanobodies
type
single-domain
antibody
have
been
discovered
recent
years.
They
can
be
expressed
large
scale
through
prokaryotic
expression
systems,
makes
them
cost-effective,
stable,
less
immunogenic.
This
study
used
phage
display
library
nanobodies
against
protein.
After
rounds
selection
enrichment,
DNA
sequence
specific
nanobody
S1Nb1
was
successfully
obtained.
To
obtain
soluble
S1Nb1,
its
inserted
vector
Pcold
solubility-enhancing
SUMO
tag
added.
resulting
recombinant
vector,
Pcold-SUMO-S1Nb1,
then
transformed
