Treated wastewater: A hotspot for multidrug- and colistin-resistant Klebsiella pneumoniae DOI Creative Commons
Andrea Di Cesare, Alessandra Cornacchia, Tomasa Sbaffi

et al.

Environmental Pollution, Journal Year: 2024, Volume and Issue: 359, P. 124598 - 124598

Published: July 23, 2024

Wastewater treatment plants are hotspots for the release of antimicrobial resistant pathogenic bacteria into aquatic ecosystems, significantly contributing to cycle resistance. Special attention should be paid ESKAPE bacteria, which have been identified as high-priority targets control measures. Among them, Klebsiella pneumoniae is particularly noteworthy. In this study, we collected wastewater samples from inlet, sedimentation tank, and effluent water a plant in June, July, October, November 2018. We detected characterized 42 K. strains using whole genome sequencing (15 8 19 effluent). Additionally, were tested their resistance phenotype. Using no distinct patterns observed terms genetic profiles. All tetracycline, meanwhile 60%, 47%, 37.5% isolated effluent, respectively, multidrug resistant. Some isolates also colistin, nearly all positive eptB arnT genes, associated with polymyxin Various genes linked mobile elements, they did not correlate virulence groups or defense systems. Overall, our results, although quantitative, highlight that strains, including those colistin genetically unrelated, being discharged ecosystems plants. This suggests necessity monitoring aimed at characterizing these bacteria.

Language: Английский

Carbapenem-Resistant Klebsiella pneumoniae: Virulence Factors, Molecular Epidemiology and Latest Updates in Treatment Options DOI Creative Commons
Theodoros Karampatakis, Katerina Tsergouli, Payam Behzadi

et al.

Antibiotics, Journal Year: 2023, Volume and Issue: 12(2), P. 234 - 234

Published: Jan. 21, 2023

Klebsiella pneumoniae is a Gram-negative opportunistic pathogen responsible for variety of community and hospital infections. Infections caused by carbapenem-resistant K. (CRKP) constitute major threat public health are strongly associated with high rates mortality, especially in immunocompromised critically ill patients. Adhesive fimbriae, capsule, lipopolysaccharide (LPS), siderophores or iron carriers the main virulence factors which contribute to pathogenicity pneumoniae. Colistin tigecycline some last resorts treatment CRKP Carbapenemase production, carbapenemase (KPC) metallo-β-lactamase (MBL), constitutes basic molecular mechanism emergence. Knowledge appearance crucial, as it can determine selection most suitable antimicrobial agent among those recently launched. Plazomicin, eravacycline, cefiderocol, temocillin, ceftolozane–tazobactam, imipenem–cilastatin/relebactam, meropenem–vaborbactam, ceftazidime–avibactam aztreonam–avibactam potent alternatives treating The aim current review highlight pathogenesis provide recent updates on epidemiology options.

Language: Английский

Citations

152
Carbapenem-resistant Klebsiella pneumoniae : the role of plasmids in emergence, dissemination, and evolution of a major clinical challenge DOI Open Access
Vincenzo Di Pilato, Simona Pollini, Vivì Miriagou

et al.

Expert Review of Anti-infective Therapy, Journal Year: 2024, Volume and Issue: 22(1-3), P. 25 - 43

Published: Jan. 18, 2024

Introduction Klebsiella pneumoniae is a major agent of healthcare-associated infections and cause some community-acquired infections, including severe bacteremic associated with metastatic abscesses in liver other organs. Clinical relevance compounded by its outstanding propensity to evolve antibiotic resistance. In particular, the emergence dissemination carbapenem resistance K. has posed challenge due few residual treatment options, which have only recently been expanded new agents. The epidemiological success carbapenem-resistant (CR-Kp) mainly linked clonal lineages that produce carbapenem-hydrolyzing enzymes (carbapenemases) encoded plasmids.

Language: Английский

Citations

20
The pivotal role of IncFIB(Mar) plasmid in the emergence and spread of hypervirulent carbapenem-resistant Klebsiella pneumoniae DOI Creative Commons
Zhewei Sun, Jianfeng Zhang, Chuning Wang

et al.

Science Advances, Journal Year: 2025, Volume and Issue: 11(5)

Published: Jan. 31, 2025

The hypervirulent carbapenem-resistant Klebsiella pneumoniae (hv-CRKP) poses a substantial challenge to the global health care. However, mechanism behind its evolution and transmission remain elusive. Here, four virulence plasmid types were identified from 310 hv-CRKP isolates collected nationwide during 2017–2018, based on their aerobactin ( iuc locus) lineage IncFIB replicons. Notably, pIUC1-IncFIB(K) 37 pIUC1-IncFIB(Mar), representing two epidemic plasmids in Asia Europe, respectively, accounted for >90% of episodes. Analysis 494 K. (376 2010–2013; 118 2017–2018) 2578 public genomes indicated notable role IncFIB(Mar) emergence spread. Conjugation assays showed helper could efficiently transfer into strain uniquely retromobilize with back CRKP. Thereafter, either lost rapidly or recombined , generating hybrid pIUC1-IncFIB(Mar) plasmid. Our findings elucidated formation, evolution, dissemination trajectories major strains different regions.

Language: Английский

Citations

2
Hypervirulent Klebsiella pneumoniae DOI Creative Commons
Junjun Chen, Huan Zhang, Xuelian Liao

et al.

Infection and Drug Resistance, Journal Year: 2023, Volume and Issue: Volume 16, P. 5243 - 5249

Published: Aug. 1, 2023

Abstract: Hypervirulent Klebsiella pneumoniae (hvKP), especially multidrug-resistant hvKP (MDR-hvKP) infections, are distributed globally, and lead to several outbreaks with high pathogenicity mortality in immunocompetent individuals. This is usually characterized by a rapidly metastatic spread resulting multiple pyogenic tissue abscesses. To date, even though the explanation of hypervirulent factors has been identified, it still remains be fully understood. The most common key virulence agents included (1) siderophore systems for iron acquisition, (2) increased capsule production, (3) colibactin toxin, (4) hypermucoviscosity, so on. Several hypervirulence have renewed, evolution MDR-hvKP deeply explored recently. We aim describe chain attributed lethality MDR-hvKP. In this review, recent advances renewed were summarized, potential therapeutic targets explored. Novel co-existence elements, superplasmid, was screened. Superplasmid simultaneously harbours genes can mobile autonomously its complete conjugative elements. Research into related immunity also gained traction, which may cause invasive infections higher rates than classical ones, such as neutrophil- complement-mediated activity. multidrug resistance accelerating. More reliable methods identifying or must investigated. Furthermore, critical investigate innovative treatment future. Keywords: , hypervirulence, resistant

Language: Английский

Citations

26
Comprehensive study reveals phenotypic heterogeneity in Klebsiella pneumoniae species complex isolates DOI Creative Commons
Nadia Rodríguez-Medina, Jonathan Rodríguez-Santiago,

Alejandro Alvarado-Delgado

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: March 11, 2024

Abstract Here, we conducted a comprehensive analysis of 356 Klebsiella pneumoniae species complex (KpSC) isolates that were classified as classical (cl), presumptive hypervirulent (p-hv) and hypermucoviscous-like (hmv-like). Overall, K. (82.3%), variicola (2.5%) quasipneumoniae identified. These comprised 321 cl-KpSC, 7 p-hv-KpSC 18 hmv-like-KpSC. A large proportion cl-KpSC extended-spectrum-β-lactamases (ESBLs)-producers (64.4%) 3.4% colistin-resistant carrying carbapenemase ESBL genes. All showed an antibiotic susceptible phenotype hmv-like found to be ESBL-producers (8/18). Assays for capsule production capsule-dependent virulence phenotypes whole-genome sequencing (WGS) performed in subset isolates. Capsule amount differed all p-hv strains produced higher amounts than cl strains; these variations had important implications phagocytosis virulence. Murine sepsis model most nonlethal the caused 100% mortality with 3 × 10 8 CFUs. Unexpectedly, 3/7 (42.9%) required CFUs cause (atypical hypervirulent), 4/7 (57.1%) considered truly (hv). Genomic analyses confirmed diverse population, including belonging hv clonal groups (CG) CG23, CG86, CG380 CG25 (this corresponded ST3999 novel clone) MDR clones such CG258 CG147 (ST392) among others. We noted hv-ST3999 close phylogenetic relationship cl-MDR K . The information collected here is understand evolution clinically ESBL-producing-hypermucoviscous-like amongst KpSC Mexican healthcare settings. Likewise, this study shows mgrB inactivation main mechanism colistin resistance from Mexico.

Language: Английский

Citations

11
Clinical use of tigecycline may contribute to the widespread dissemination of carbapenem-resistant hypervirulent Klebsiella pneumoniae strains DOI Creative Commons
Miaomiao Xie, Lianwei Ye, Kaichao Chen

et al.

Emerging Microbes & Infections, Journal Year: 2024, Volume and Issue: 13(1)

Published: Jan. 19, 2024

Emergence of carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-hvKP) poses grave threats to human health. These strains increased dramatically in clinical settings China the past few years but not other parts world. Four isogenic K. strains, including classical pneumoniae, (CRKP), (hvKP) and CR-hvKP, were created subjected phenotypic characterization, competition assays, mouse sepsis model rat colonization tests investigate mechanisms underlying wide spread CR-hvKP China. Acquisition virulence plasmid led reduced fitness abolishment gastrointestinal tract, which may explain why hvKP are clinically prevalent after its emergence for a long time. However, tigecycline treatment facilitated population Lactobacillus spp. animal gut microbiome. Feeding with could significantly reduce tract. Our data implied that use treat infections demonstrated was potential candidate anticolonization strategy against CR-hvKP.

Language: Английский

Citations

10
Characterisation of microbial communities and quantification of antibiotic resistance genes in Italian wastewater treatment plants using 16S rRNA sequencing and digital PCR DOI Creative Commons
Giusy Bonanno Ferraro, Carmelo Bonomo, David Brandtner

et al.

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 933, P. 173217 - 173217

Published: May 13, 2024

The spread of antibiotic resistant bacteria (ARB) and resistance genes (ARGs) in humans, animals environment is a growing threat to public health. Wastewater treatment plants (WWTPs) are crucial mitigating the risk environmental contamination by effectively removing contaminants before discharge. However, persistence ARB ARGs even after challenge for management water system. To comprehensively assess antimicrobial dynamics, we conducted one-year monitoring study three WWTPs central Italy, both influents effluents. We used seasonal sampling analyze microbial communities 16S rRNA, as well determine prevalence behaviour major (sul1, tetA, bla

Language: Английский

Citations

9
Transmission Dynamics and Novel Treatments of High Risk Carbapenem-Resistant Klebsiella pneumoniae: The Lens of One Health DOI Creative Commons

Jiaying Zhu,

Taoyu Chen, Yanmin Ju

et al.

Pharmaceuticals, Journal Year: 2024, Volume and Issue: 17(9), P. 1206 - 1206

Published: Sept. 12, 2024

The rise of antibiotic resistance and the dwindling antimicrobial pipeline have emerged as significant threats to public health. emergence carbapenem-resistant

Language: Английский

Citations

9
Rapid emergence, transmission, and evolution of KPC and NDM coproducing carbapenem-resistant Klebsiella pneumoniae DOI
Jiayang Li,

Wenqi Wu,

Hao Wu

et al.

Microbiological Research, Journal Year: 2025, Volume and Issue: 293, P. 128049 - 128049

Published: Jan. 5, 2025

Language: Английский

Citations

1
Genomic Analysis of Virulent, Multidrug Resistant Klebsiella pneumoniae and Klebsiella oxytoca from Bloodstream Infections, South Africa DOI Creative Commons
Bakoena A. Hetsa, Jonathan Asante, Joshua Mbanga

et al.

Microbial Pathogenesis, Journal Year: 2025, Volume and Issue: unknown, P. 107272 - 107272

Published: Jan. 1, 2025

The study investigated the resistome, virulome and mobilome of multidrug resistant (MDR) Klebsiella pneumoniae oxytoca clinical isolates. A total 46 suspected species (spp.) were collected from blood cultures within uMgungundlovu District in KwaZulu-Natal Province. Antibiotic susceptibility was determined against a panel 19 antibiotics using disk diffusion test. subset 14 MDR K. (n=10) (n=4) isolates selected based on their antibiograms subjected to whole genome sequencing (WGS). sequence types (STs), virulome, mobilome, capsule loci (KLs) analysed relevant WGS bioinformatics tools. Of 10 (ST) identified, most common ST25 (n=3), ST101 4 belonged ST450 (n=3). two high-risk clones ST15, ST17 identified. O K with predominance KL2, KL17, KL29, O1/O2v2, O1/O2v1, OL104 respectively. majority displayed resistance predominantly carrying β-lactamase genes, including bla convergence hypervirulence genes strains is potential concern. Carbapenemase, ESBL screening genomic surveillance are urgently required hospital environments.

Language: Английский

Citations

1