Resveratrol inhibits Lin28A expression and induces its degradation via the proteasomal pathway in NCCIT cells DOI Open Access

Sandra Cotino‐Nájera,

Enrique García‐Villa,

Samantha Cruz‐Rosales

et al.

Oncology Letters, Journal Year: 2024, Volume and Issue: 28(6)

Published: Sept. 30, 2024

Lin28A is an oncoprotein overexpressed in several cancer types such as testicular, ovarian, colon, breast and lung cancers. As a pluripotency factor that promotes tumorigenesis, associated with more undifferentiated aggressive tumors phenotypes. Moreover, highly stable protein difficult to downregulate. The compound resveratrol (RSV) has anticancer effects. present study aimed elucidate the mechanisms underlying downregulation of expression by RSV NCCIT cell line. cells were treated different concentrations investigate its effects on expression. mRNA levels ubiquitin-specific protease 28 (USP28) assessed using reverse transcription-quantitative PCR. Western blot analysis was employed evaluate Lin28A, USP28 phosphorylated Lin28A. In addition, some experiments, MAPK/ERK pathway inhibitor, other experiments involved transfecting small interfering RNAs targeting USP28. results demonstrated significantly reduced destabilizing protein; this effect mediated ability suppress USP28, deubiquitinase normally protects from ubiquitination degradation. Additionally, inhibited phosphorylation via pathway; event previously been shown enhance stability increasing half-life. This resulted degradation through proteasomal cells. provide further evidence activity RSV, identified promising therapeutic targets. oncoprotein, downregulating challenging. However, can overcome hurdle inhibiting signaling promote Furthermore, elucidating these provides avenues for developing targeted therapies.

Language: Английский

The pathological mechanisms and potential therapeutic drugs for myocardial ischemia reperfusion injury DOI
Shuo Zhang, Fei Yan, Fei Luan

et al.

Phytomedicine, Journal Year: 2024, Volume and Issue: 129, P. 155649 - 155649

Published: April 17, 2024

Language: Английский

Citations

17
Puerarin pretreatment provides protection against myocardial ischemia/reperfusion injury via inhibiting excessive autophagy and apoptosis by modulation of HES1 DOI Creative Commons

Yong Yuan,

Songqing Lai, Tie Hu

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 4, 2025

The study aimed to elucidate the underlying pharmacological mechanism of traditional Chinese medicine Pue in ameliorating myocardial ischemia-reperfusion injury (MIRI), a critical clinical challenge exacerbated by reperfusion therapy. In vivo MIRI and vitro anoxia/reoxygenation (A/R) models were constructed. results demonstrated that pretreatment effectively alleviated MIRI, as manifested diminishing levels serum CK-MB LDH, mitigating extent infarction enhancing cardiac functionality. Additionally, significantly histopathological damage MIRI-treated myocardium, evidenced HE staining TUNEL assay. vitro, A/R-induced decreasing LDH levels, increasing cellular activity, inhibiting autophagic lysosomal overactivation, oxidative stress (ROS, LIP ROS, MDA), antioxidant defense (SOD, GSH-Px), P62 protein expression while LC3II/I ratio. Furthermore, inhibited apoptosis maintained mitochondrial homeostasis up-regulating Hairy Enhancer Split-1 (HES1) protein, which was crucial for its cardioprotective effects. Nevertheless, efficacy negated via knockdown HES1 pAD/HES1-shRNA transfection. conclusion, ameliorated HES1-mediated MIRI-induced autophagy, apoptosis, dysfunction.

Language: Английский

Citations

2
Different types of cell death and their interactions in myocardial ischemia–reperfusion injury DOI Creative Commons

Bingxin Du,

Qiang Fu, Qin Yang

et al.

Cell Death Discovery, Journal Year: 2025, Volume and Issue: 11(1)

Published: March 5, 2025

Myocardial ischemia-reperfusion (I/R) injury is a multifaceted process observed in patients with coronary artery disease when blood flow restored to the heart tissue following ischemia-induced damage. Cardiomyocyte cell death, particularly through apoptosis, necroptosis, autophagy, pyroptosis, and ferroptosis, pivotal myocardial I/R injury. Preventing death during of vital for improving ischemic cardiomyopathy. These multiple forms can occur simultaneously, interact each other, contribute complexity In this review, we aim provide comprehensive summary key molecular mechanisms regulatory patterns involved these five types We will also discuss crosstalk intricate interactions among mechanisms, highlighting interplay between different death. Furthermore, explore specific molecules or targets that participate pathways elucidate their action. It important note manipulating distinct processes may have significant impact on reducing By enhancing researchers' understanding injury, review aims pave way development novel interventions cardio-protection affected by

Language: Английский

Citations

1
Multidimensional biological activities of resveratrol and its prospects and challenges in the health field DOI Creative Commons

Xi Yu,

Yuanqiang Jia,

Feiyue Ren

et al.

Frontiers in Nutrition, Journal Year: 2024, Volume and Issue: 11

Published: June 12, 2024

Resveratrol (RES) is a naturally occurring polyphenolic compound. Recent studies have identified multiple potential health benefits of RES, including antioxidant, anti-inflammatory, anti-obesity, anticancer, anti-diabetic, cardiovascular, and neuroprotective properties. The objective this review to summarize analyze the on biological activities RES in disease prevention treatment, as well its metabolism bioavailability. It also discusses challenges clinical application future research directions. exhibits significant treatment many diseases. direction should focus improving bioavailability, conducting more trials determine effectiveness humans, investigating mechanism action. Once these been overcome, expected become an effective intervention.

Language: Английский

Citations

6
In vivo and in vitro study on the regulatory mechanism of XiaoChaiHu decoction on PANoptosis in sepsis-induced cardiomyopathy DOI
Yaru Wang,

Xingxing Fu,

Shang Zhao

et al.

Journal of Ethnopharmacology, Journal Year: 2024, Volume and Issue: 336, P. 118740 - 118740

Published: Aug. 27, 2024

Language: Английский

Citations

4
The PINK1/Parkin signaling pathway-mediated mitophagy: a forgotten protagonist in myocardial ischemia/reperfusion injury DOI Creative Commons
Xiaopeng Zhao, Zheng Wang, Lijie Wang

et al.

Pharmacological Research, Journal Year: 2024, Volume and Issue: 209, P. 107466 - 107466

Published: Oct. 15, 2024

Myocardial ischemia causes extensive damage, further exacerbated by reperfusion, a phenomenon called myocardial ischemia/reperfusion injury (MIRI). Nowadays, the pathological mechanisms of MIRI have received attention. Oxidative stress, multiple programmed cell deaths, inflammation and others are all essential contributing to MIRI. Mitochondria energy supply centers cells. Numerous studies found that abnormal mitochondrial function is an "culprit" MIRI, mitophagy mediated phosphatase tensin homolog (PTEN)-induced kinase 1 (PINK1)/Parkin signaling pathway integral part maintaining function. Therefore, exploring association between PINK1/Parkin pathway-mediated crucial. This review will mainly summarize crucial role in MIR-induced several various potential interventions affect mitophagy, thus ameliorating

Language: Английский

Citations

4
Sephin1 enhances integrated stress response and autophagy to alleviate myocardial ischemia-reperfusion injury in mice DOI Open Access
Yong Wu, Huabin Zhang,

Yue Wang

et al.

Biomedicine & Pharmacotherapy, Journal Year: 2024, Volume and Issue: 176, P. 116869 - 116869

Published: June 7, 2024

Integrated stress response (ISR) is activated to promote cell survival by maintaining the phosphorylation of eukaryotic translation initiation factor 2 (eIF2α). We investigated whether Sephin1 enhances ISR and attenuates myocardial ischemia-reperfusion (MIR) injury.

Language: Английский

Citations

3
Impact of plant-derived antioxidants on heart aging: a mechanistic outlook DOI Creative Commons
Muneer Ahmed Khoso, Heng Liu, Tong Zhao

et al.

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16

Published: March 21, 2025

Heart aging involves a complex interplay of genetic and environmental influences, leading to gradual deterioration cardiovascular integrity function. Age-related physiological changes, including ventricular hypertrophy, diastolic dysfunction, myocardial fibrosis, increased arterial stiffness, endothelial are influenced by key mechanisms like autophagy, inflammation, oxidative stress. This review aims explore the therapeutic potential plant-derived bioactive antioxidants in mitigating heart aging. These compounds, often rich polyphenols, flavonoids, other phytochemicals, exhibit notable antioxidant, anti-inflammatory, cardioprotective properties. substances have intricate properties, ability scavenge ROS, enhance endogenous antioxidant defenses, regulate signaling pathways, impede fibrosis inflammation-promoting processes. By focusing on molecular linked cardiac aging, produced from plants provide significant promise reduce age-related decline improve general health. Through comprehensive analysis preclinical clinical studies, this work highlights associated with promising effects antioxidants. The findings may helpful for researchers identifying specific molecules preventive heart.

Language: Английский

Citations

0
Interplay Between Polyphenols and Autophagy: Insights From an Aging Perspective DOI Creative Commons
Alejandro Ponce‐Mora, Nelson Bautista Salazar, Alicia Domenech‐Bendaña

et al.

Frontiers in Bioscience-Landmark, Journal Year: 2025, Volume and Issue: 30(3)

Published: March 19, 2025

The relationship between polyphenols and autophagy, particularly in the context of aging, presents a promising avenue for therapeutic interventions age-related diseases. A decline autophagy is associated with aging-related affections, an increasing number studies suggest that this enhancement linked to cellular resilience longevity. This review delves into multifaceted roles homeostasis potential modulate autophagic pathways. We revised most updated literature regarding modulatory effects on cardiovascular, liver, kidney diseases, highlighting their potential. highlight role as modulators combat thus contributing improving quality life aging populations. better understanding interplay will help pave way future research clinical applications field longevity medicine.

Language: Английский

Citations

0
Verbascoside attenuates myocardial ischemia/reperfusion-induced ferroptosis following heterotopic heart transplantation via modulating GDF15/GPX4/SLC7A11 pathway DOI
Yuxi Zhang, Jing Zhan, Zhen Qiu

et al.

Published: April 9, 2025

Abstract Myocardial cold ischemia/reperfusion (I/R) injury is an inevitable consequence of heart transplantation, significantly affecting survival rates and therapeutic outcomes. Growth Differentiation Factor 15 (GDF15) has been shown to regulate GPX4-mediated ferroptosis, playing a critical role in mitigating I/R injury. Meanwhile, verbascoside (VB), active compound extracted from the herbaceous plant, demonstrated myocardial protective effects. In this study, transplantation was performed using modified non-suture cuff technique, with VB administered at dose 20 mg/kg/day via intraperitoneal injection for 3 days vivo. vitro, cardiomyocytes were pretreated 50 µg/ml 24 hours. treatment reduced histopathological injury, decreased markers, inhibited ferroptosis oxidative stress during vitro experiments further that GDF15 alleviates induced by hypoxic reoxygenation upregulating GPX4. Therefore, it concluded preconditioning can effectively reduce after ectopic possibly through up-regulation GDF15/GPX4/SLC7A11 pathway.

Language: Английский

Citations

0