Acta Biotheoretica, Journal Year: 2025, Volume and Issue: 73(1)
Published: Feb. 11, 2025
Language: Английский
Eurosurveillance, Journal Year: 2023, Volume and Issue: 28(25)
Published: June 22, 2023
Background Surveillance of SARS-CoV-2 in wastewater offers a near real-time tool to track circulation at local scale. However, individual measurements sewage are noisy, inherently variable and can be left-censored. Aim We aimed infer latent virus loads comprehensive surveillance programme that includes all treatment plants (STPs) the Netherlands covers 99.6% Dutch population. Methods applied multilevel Bayesian penalised spline model estimate time- STP-specific based on water flow-adjusted qRT-PCR data for one four samples per week each more than 300 STPs. Results The captured epidemic upsurges downturns Netherlands, despite substantial day-to-day variation measurements. Estimated STP varied by two orders magnitude, from ca 10 12 particles 100,000 persons day trough August 2020 almost 15 many STPs January 2022. timing epidemics level was slightly shifted between municipalities, which resulted less pronounced peaks troughs national level. Conclusion Although is observed load measurements, wastewater-based performed high sampling frequency long-term progression an scale real time.
Language: Английский
Citations
17
Journal of Environmental Exposure Assessment, Journal Year: 2024, Volume and Issue: 3(1)
Published: Feb. 26, 2024
Antimicrobial resistance (AMR) is recognized as one of the most serious threats to public health. Unparalleled population growth and accelerated rates AMR emergence dissemination have resulted in both novel pathogenic organisms re-appearance infections that were formerly under control. Consequently, this has led an increased quantity infectious diseases. One main drivers antimicrobial overuse inappropriate prescribing human veterinary medicine. The ability rapidly survey spread within populations key for its prevention, intervention, However, many constraints are present current clinical surveillance systems their capacity determine dynamics microbiome healthy individuals well pathogens causing infections. Wastewater-based epidemiology (WBE) emergent technique act a supplementary measure disease early warning system outbreaks. development outbreaks community level can be monitored real time through analysis pooled wastewater. This review provides introduction using wastewater-based monitor bacteria, overview components.
Language: Английский
Citations
8
The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 933, P. 173108 - 173108
Published: May 9, 2024
Wastewater-based surveillance (WBS) has shown to be an effective tool in monitoring the spread of SARS-CoV-2 and helped guide public health actions. Consequently, WBS expanded now include mpox virus (MPXV) contribute its mitigation efforts. In this study, we demonstrate a unique sample processing molecular diagnostic strategy for MPXV detection that can inform on epidemiological situation outbreaks through WBS. We conducted 22 Canadian wastewater treatment plants (WWTPs) 14 weeks. Three qPCR assays were assessed study which G2R (G2R_WA G2R_G) developed by Centers Disease Control Prevention (CDC) 2010, in-house-developed assay have termed G2R_NML. The G2R_NML was designed using reference genomes from 2022 outbreak provides larger amplicon size facilitate Sanger sequencing. Results show all three similar limits are able detect presence wastewater. produced significantly greater number sequence-confirmed results compared CDC assays. Detection possible where provincial indicated overall low caseloads, some sites forewarning up several weeks observed. Overall, proposes additional information help fill knowledge gaps clinical case-surveillance is potentially essential component management mpox.
Language: Английский
Citations
7
Epidemics, Journal Year: 2024, Volume and Issue: 48, P. 100784 - 100784
Published: July 31, 2024
The COVID-19 pandemic demonstrated the key role that epidemiology and modelling play in analysing infectious threats supporting decision making real-time. Motivated by unprecedented volume breadth of data generated during pandemic, we review modern opportunities for analysis to address questions emerge a major epidemic. Following broad chronology insights required - from understanding initial dynamics retrospective evaluation interventions, describe theoretical foundations each approach underlying intuition. Through series case studies, illustrate real life applications, discuss implications future work.
Language: Английский
Citations
6
Value in Health, Journal Year: 2022, Volume and Issue: 26(4), P. 508 - 518
Published: Nov. 26, 2022
Language: Английский
Citations
25
Infectious Disease Modelling, Journal Year: 2023, Volume and Issue: 8(4), P. 1138 - 1150
Published: Oct. 31, 2023
The public health response to COVID-19 has shifted reducing deaths and hospitalizations prevent overwhelming systems. amount of SARS-CoV-2 RNA fragments in wastewater are known correlate with clinical data including cases hospital admissions for COVID-19. We developed tested a predictive model incident New York State using data. Using county-level surveillance covering 13.8 million people across 56 counties, we fit generalized linear mixed predicting new from concentrations April 29, 2020 June 30, 2022. included covariates such as vaccine coverage the county, comorbidities, demographic variables, holiday gatherings. Wastewater correlated per 100,000 up ten days prior admission. Models that had higher power than models only, increasing accuracy by 15%. Predicted highly observed (r = 0.77) an average difference 0.013 (95% CI [0.002, 0.025]) predict future is accurate effective superior results case alone. lead time could alert take precautions improve resource allocation seasonal surges.
Language: Английский
Citations
13
BMC Infectious Diseases, Journal Year: 2024, Volume and Issue: 24(1)
Published: Jan. 29, 2024
Abstract Background The spread of SARS-CoV-2 has been studied at unprecedented levels worldwide. In jurisdictions where molecular analysis was performed on large scales, the emergence and competition numerous SARS-CoV-2lineages have observed in near real-time. Lineage identification, traditionally from clinical samples, can also be determined by sampling wastewater sewersheds serving populations interest. Variants concern (VOCs) lineages associated with increased transmissibility and/or severity are particular Method Here, we consider data sources to assess VOCs Canada retrospectively. Results We show that, overall, wastewater-based VOC identification provides similar insights surveillance based samples. Based data, synchrony introduction as well speeds across most Canadian provinces despite geographical size country differences provincial public health measures. Conclusion particular, it took approximately four months for Alpha Delta contribute half incidence. contrast, Omicron achieved same contribution less than one month. This study significant benchmarks enhance planning future VOCs, some extent pandemics caused other pathogens, quantifying rate invasion Canada.
Language: Английский
Citations
5
Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)
Published: June 22, 2024
Wastewater based epidemiology has become a widely used tool for monitoring trends of concentrations different pathogens, most notably and widespread SARS-CoV-2. Therefore, in 2022, also Rhineland-Palatinate, the Ministry Science Health included 16 wastewater treatment sites surveillance program providing biweekly samples. However, mere viral load data is subject to strong fluctuations limited value political deciders on its own. state Rhineland-Palatinate commissioned University Medical Center at Johannes Gutenberg Mainz conduct representative cohort study called SentiSurv, which an increasing number up 12,000 participants have been using sensitive antigen self-tests once or twice week test themselves SARS-CoV-2 report their status. This puts fortunate position having time series both, prevalence population. Our main contribution calibration from 2023-01-08 until 2023-10-01 where we identified scaling factor ( 0.208±0.031 ) delay 5.07±2.30 days) between virus wastewater, normalized by pepper mild mottle (PMMoV), recorded SentiSurv study. The relation established fitting epidemiological model both series. We show how that can be estimate when no longer available use it as forecasting instrument several weeks ahead time. quality resulting factors depend strongly samples are normalized.
Language: Английский
Citations
5
Epidemics, Journal Year: 2024, Volume and Issue: 49, P. 100793 - 100793
Published: Sept. 26, 2024
Language: Английский
Citations
5
FACETS, Journal Year: 2022, Volume and Issue: 7, P. 1493 - 1597
Published: Jan. 1, 2022
Wastewater surveillance for SARS-CoV-2 RNA is a relatively recent adaptation of long-standing wastewater infectious and other harmful agents. Individuals infected with COVID-19 were found to shed in their faeces. Researchers around the world confirmed that fragments could be detected quantified community wastewater. Canadian academic researchers, largely as volunteer initiatives, reported proof-of-concept by April 2020. National collaboration was initially facilitated Water Network. Many public health officials skeptical about actionable information being provided even though experience has shown pandemic no single, perfect approach. Rather, different approaches provide insights, each its own strengths limitations. Public science must triangulate among forms evidence maximize understanding what happening or may expected. Well-conceived, resourced, implemented wastewater-based platforms can cost-effective approach support conventional lines evidence. Sustaining monitoring future disease targets states challenge. Canada benefit from taking lessons learned develop forward-looking interpretive frameworks capacity implement, adapt, expand such capabilities.
Language: Английский
Citations
21