Food Chemistry, Journal Year: 2024, Volume and Issue: 468, P. 142482 - 142482
Published: Dec. 14, 2024
Language: Английский
Food Chemistry, Journal Year: 2024, Volume and Issue: 468, P. 142482 - 142482
Published: Dec. 14, 2024
Language: Английский
LWT, Journal Year: 2025, Volume and Issue: 218, P. 117443 - 117443
Published: Feb. 1, 2025
Language: Английский
Citations
0Biotechnology and Bioengineering, Journal Year: 2024, Volume and Issue: unknown
Published: Sept. 11, 2024
Abstract Sucrose is a commonly utilized nutritive sweetener in food and beverages due to its abundance nature low production costs. However, excessive intake of sucrose increases the risk metabolic disorders, including diabetes obesity. Therefore, there growing demand for development nonnutritive sweeteners with almost no calories. d ‐Allulose an ultra‐low‐calorie, rare six‐carbon monosaccharide high sweetness, making it ideal alternative sucrose. In this study, we developed cell factory ‐allulose from using Escherichia coli JM109 (DE3) as chassis host. The genes cscA , cscB cscK alsE a6PP were co‐expressed construction synthesis pathway. Then, introduction ptsG‐F knockout ptsG fruA ptsI ptsH reprogram sugar transport pathways resulted improvement substrate utilization. Next, carbon fluxes Embden‐Meyerhof‐Parnas pentose phosphate regulated by inactivation pfkA zwf achieving increase titer yield 154.2% 161.1%, respectively. Finally, scaled‐up fermentation was performed 5 L fermenter. reached 11.15 g/L, 0.208 g/g on
Language: Английский
Citations
2International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: unknown, P. 138514 - 138514
Published: Dec. 1, 2024
Language: Английский
Citations
2International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 282, P. 137326 - 137326
Published: Nov. 6, 2024
Language: Английский
Citations
1Food Chemistry, Journal Year: 2024, Volume and Issue: 468, P. 142482 - 142482
Published: Dec. 14, 2024
Language: Английский
Citations
0