Systematic review of preclinical evidence reveals the anti-inflammatory potential of Icariin based on meta-analysis and machine learning DOI Open Access
Xiaochuan Guo,

Yanqin Qin,

Zhenzhen Feng

et al.

Authorea (Authorea), Journal Year: 2023, Volume and Issue: unknown

Published: Aug. 11, 2023

Purpose: The aim of this study was to construct a reference system for preclinical evidence and drug superiority characterization the anti-inflammatory effects icariin glycosides their derivatives through meta-analysis combined with machine learning, excavate biological mechanisms behind them. Methods: data were obtained from databases PubMed, Embase, Cochrane Library, Web Science. STATA software used indicators, subgroup analysis conducted based on animal species, gender, type disease, dosage, course obtain more particulars. Furthermore, model construction performed using R explore influential features efficacy. In addition, pharmacological its in anti-inflammation summarized comprehensive understanding relevant literature. Results: After searching screeningThe results showed that significantly inhibit inflammation indicators such as TNF-α IL-1β. Besides, learning output variable had stronger when disease respiratory, urological, neurological, digestive, dose duration Icariin greater than 27.52 mg/kg/day 31.22 days, respectively. Conclusion: demonstrate strong effects, particularly urinary, digestive disorders. When given at doses or more, treatment lasting days beyond, these compounds hold significant potential drugs inhibition across multiple dis

Language: Английский

The neuroprotective mechanisms of naringenin: Inhibition of apoptosis through the PI3K/AKT pathway after hypoxic-ischemic brain damage DOI
Luyao Li, Zhen Lin, Junhui Yuan

et al.

Journal of Ethnopharmacology, Journal Year: 2023, Volume and Issue: 318, P. 116941 - 116941

Published: July 20, 2023

Language: Английский

Citations

39
The Influence of Oxidative Stress Markers in Patients with Ischemic Stroke DOI Creative Commons
Hanna Pawluk, Agnieszka Tafelska‐Kaczmarek,

Małgorzata Sopońska

et al.

Biomolecules, Journal Year: 2024, Volume and Issue: 14(9), P. 1130 - 1130

Published: Sept. 6, 2024

Stroke is the second leading cause of death worldwide, and its incidence rising rapidly. Acute ischemic stroke a subtype that accounts for majority cases has high mortality rate. An effective treatment to minimize damage brain's neural tissue by restoring blood flow decreased perfusion areas brain. Many reports have concluded both oxidative stress excitotoxicity are main pathological processes associated with stroke. Current measures protect brain against serious caused insufficient. For this reason, it important investigate antioxidant strategies reduce damage. This review focuses on studies assessing concentration biomarkers level antioxidants (enzymatic non-enzymatic) their impact clinical prognosis patients after Mechanisms related production ROS/RNS role in pathogenesis presented, as well new therapeutic aimed at reducing effects ischemia reperfusion.

Language: Английский

Citations

7
Neural and immune roles in osteoarthritis pain: Mechanisms and intervention strategies DOI Creative Commons

Zou Yi,

Changyu Liu, Zhenggang Wang

et al.

Journal of Orthopaedic Translation, Journal Year: 2024, Volume and Issue: 48, P. 123 - 132

Published: Aug. 7, 2024

Pain is the leading symptom for most individuals with osteoarthritis (OA), a complex condition marked by joint discomfort. Recently, dynamic interplay between nervous and immune systems has become focal point understanding pain regulation. Despite this, there still substantial gap in our comprehensive of neuroimmune interactions their effects on OA. This review examines bidirectional influences cells nerves OA progression. It explores current approaches that target pathways, including promoting M2 macrophage polarization specific neuronal receptor targeting, effective reduction.

Language: Английский

Citations

5
Depletion of Arg1-Positive Microglia/Macrophages Exacerbates Cerebral Ischemic Damage by Facilitating the Inflammatory Response DOI Open Access
Ting Li, Jin Zhao, Hao Gao

et al.

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(21), P. 13055 - 13055

Published: Oct. 27, 2022

Stroke is a serious worldwide disease that causes death and disability, more than 80% of which ischemic stroke. The expression arginase 1 (Arg1), key player in regulating nitrogen homeostasis, altered the peripheral circulation after Growing evidence indicates stroke also induces upregulated Arg1 central nervous system, especially activated microglia macrophages. This implies may affect progression by modulating cerebral immune response. To investigate effect Arg1+ microglia/macrophages on stroke, we selectively eliminated mannosylated clodronate liposomes (MCLs) investigated their effects behavior, neurological deficits, inflammatory responses mice More half cells, mainly microglia/macrophages, were depleted MCLs administration, resulting significant deterioration motility mice. After elimination infarct volume expanded neuronal degenerative lesions intensified. Meanwhile, absence significantly increased production pro-inflammatory cytokines suppressed anti-inflammatory factors, thus profoundly altering microenvironment at lesion site. Taken together, our data demonstrate depletion exacerbates damage facilitating response, leading to severe injury. These results suggest as subpopulation inflammation, beneficial controlling development ischemia promoting recovery from Regulation right time be potential target for treatment brain

Language: Английский

Citations

21
Resveratrol Improves Paclitaxel-Induced Cognitive Impairment in Mice by Activating SIRT1/PGC-1α Pathway to Regulate Neuronal State and Microglia Cell Polarization DOI Creative Commons
Xin Liu, Miao Tang,

Tian-Yi He

et al.

Drug Design Development and Therapy, Journal Year: 2023, Volume and Issue: Volume 17, P. 1125 - 1138

Published: April 1, 2023

This study aimed to investigate the effect of resveratrol (Res) on paclitaxel (PTX)-induced cognitive impairment and elucidate underlying molecular mechanisms. Morris Water Maze (MWM) test was used evaluate mice's spatial learning memory abilities. Western blotting applied detect protein expression receptor-interacting (RIP3), mixed lineage kinase domain-like (MLKL), silencing information regulator 2 related enzyme 1 (SIRT1), peroxisome proliferator activated receptor coactivator-1 (PGC-1α), NADPH oxidase (NOX2), NOX4, postsynaptic density zone 95 (PSD95), arginase-1 (Arg-1) inducible nitric oxide synthase (iNOS). Immunofluorescence RIP3, MLKL, Arg-1, Iba-1 iNOS conducted observe apoptosis hippocampal cells polarization microglia. qRT-PCR performed BDNF mRNA expressions. DHE staining assess level oxidative stress response. Golgi-Cox dendritic spine counting were visualize synaptic structural plasticity. Postsynaptic by transmission electron microscope. ELISA contents tumour necrosis factor alpha (TNF-α), IL-1β, IL-4, IL-10. PTX-induced model constructed after application PTX, represented as longer latency platform less crossing times over whole period in PTX group. After Res treatment, above indicators reversed, indicating that function improved. Moreover, reduced neuronal through SIRT1/PGC-1α pathway mice, manifesting down-regulated NOX2 NOX4. Meanwhile, increased spines PSD95 BDNF, thereby ameliorating induced damage. Besides, M2 microglia majority, eliciting anti-inflammatory cytokines IL-4 IL-10 treatment PTX+Res group, while immunofluorescence images results demonstrated an decrease proportion a following SIRT1 inhibitor EX-527. improves mice activating pathways regulate state cell polarization.

Language: Английский

Citations

12
Shionone protects cerebral ischemic injury through alleviating microglia-mediated neuroinflammation DOI

Lushan Xu,

Chenggang Li, Chenchen Zhao

et al.

Chinese Journal of Natural Medicines, Journal Year: 2025, Volume and Issue: 23(4), P. 471 - 479

Published: April 1, 2025

Language: Английский

Citations

0
A near-infrared fluorescent probe selectively recognizing cysteine to release H2S and its applications DOI
Xuan Wang,

Nianwei Wang,

Yikun Ren

et al.

The Analyst, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Cysteine, a pivotal biothiol vital for human health, exhibits strong associations with various diseases, yet its concentration assessment is often confounded by structurally and functionally similar biothiols. Hydrogen sulfide (H2S), an endogenous gasotransmitter therapeutic potential, faces challenges in achieving pharmacological effects due to the unpredictable presence of inorganic H2S donors. To address these issues, we developed TMN-ONCS, near-infrared fluorescent probe that integrates p-tolyl isothiocyanate dicyanoisophorone fluorophore through self-immolative spacer. TMN-ONCS demonstrates high selectivity analytical performance cysteine, avoids generation multiple fluorophores seen conventional probes, functions as cysteine-activated donor concurrent visualization. Furthermore, it mitigates lipopolysaccharide-induced inflammation RAW 264.7 cells, highlighting potential treating inflammation.

Language: Английский

Citations

0
Ginsenoside Rg3 attenuates neuroinflammation and hippocampal neuronal damage after traumatic brain injury in mice by inactivating the NF-kB pathway via SIRT1 activation DOI
Xi Liu,

Jia Gu,

Cheng Wang

et al.

Cell Cycle, Journal Year: 2024, Volume and Issue: 23(6), P. 662 - 681

Published: March 18, 2024

This investigation examined the potential of ginsenoside Rg3 in addressing traumatic brain injury (TBI). A TBI mouse model underwent treatment with and nicotinamide (NAM). Neurological motor functions were assessed using modified neurological severity score rotarod tests. Brain water content mice was detected. Primary microglia exposed to lipopolysaccharide (LPS), Rg3, NAM. Nissl immunofluorescence staining utilized investigate hippocampal damage, localization P65, Iba1 INOS microglia. Hippocampal neurons grown a culture medium derived from CCK-8 TUNEL assays employed evaluate viability apoptosis neurons. Proinflammatory factors proteins tested ELISA, western blot staining. As result, enhanced post-TBI, reduced content, alleviated neuronal neuroinflammation activated SIRT1, deactivated NF-kB pathway. In LPS-stimulated microglia, diminished inflammation, pathway, facilitated nuclear P65 co-localization INOS. The effects countered by NAM both cultured containing LPS, NAM-treated showed improved compared those LPS Rg3-treated alone. Ginsenoside effective reducing damage following modulating SIRT1/NF-kB suggesting its as therapeutic agent for TBI.

Language: Английский

Citations

3
Solasonine ameliorates cerebral ischemia-reperfusion injury via suppressing TLR4/MyD88/NF-κB pathway and activating AMPK/Nrf2/HO-1 pathway DOI

Kang Huo,

Jing Xu,

Meng Wei

et al.

International Immunopharmacology, Journal Year: 2023, Volume and Issue: 124, P. 110862 - 110862

Published: Sept. 5, 2023

Language: Английский

Citations

4
Effect of probenecid on astrocyte activation in vitro DOI Creative Commons

И. И. Бабкина,

VV Mazeeva,

MP Morozova

et al.

Bulletin of Russian State Medical University, Journal Year: 2024, Volume and Issue: 2024(1)

Published: Feb. 1, 2024

Both acute brain injuries and neurodegenerative diseases are accompanied by neuroinflammation. The outcome of neuroinflammation the prognosis functional status depend on balance pro-inflammatory anti-inflammatory factors. Many studies aimed at finding possible therapeutic targets allowing to shift inflammatory response processes towards mechanisms. It has been shown that channels formed pannexin proteins expressed in all cells including astrocytes. However, their role is still unclear. Channels 1 (Panx1) may be involved proinflammatory activation astrocytes induced thrombin and/or lipopolysaccharide (LPS). aim this study was assess thrombin- LPS-induced primary mouse cortical under Panx1 blockade probenecid. Functional profile astrocytes, proliferation secretory activity changed both case application (50 nM 100 nM) incubating with LPS. observed increasing nitric oxide (NO), β-hexosaminidase HEX IL6 secretion stopped after were treated Based obtained results, probenecid can considered as a potential agent influencing process tissue stabilizing through inactivation reduction astrogliosis.

Language: Английский

Citations

1