Chlorpyrifos-mediated mitochondrial calcium overload induces EPC cell apoptosis via ROS/AMPK/ULK1

Fish & Shellfish Immunology, Journal Year: 2023, Volume and Issue: 141, P. 109053 - 109053

Published: Sept. 1, 2023

Language: Английский

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Ferritinophagy Is Critical for Deoxynivalenol-Induced Liver Injury in Mice

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(12), P. 6660 - 6671

Published: March 19, 2024

Background: Deoxynivalenol (DON) contamination, pervasive throughout all stages of food production and processing, presents a significant threat to human health. The degradation ferritin mediated by nuclear receptor coactivator 4 (NCOA4), termed ferritinophagy, plays crucial role in maintaining iron homeostasis regulating ferroptosis. Aim: This study aims elucidate the ferritinophagy ferroptosis DON-induced liver injury. Methods: Male mice AML12 cells were subjected varying doses DON, serving as vivo vitro models, respectively. Protein expression was assessed using immunofluorescence western blot techniques. Co-immunoprecipitation employed investigate protein–protein interactions. Results: Our findings demonstrate that DON triggers hepatocyte ferritinophagy-dependent manner. Specifically, impedes activation mammalian target rapamycin complex 1 (mTORC1) inhibiting RAC1's binding mTOR, thereby ultimately inducing autophagy. Concurrently, amplifies NCOA4's affinity for facilitating NCOA4 phosphorylation through ataxia-telangiectasia mutated kinase (ATM), thus promoting autophagy-dependent ferritin. Both autophagy inhibition suppression ameliorate Conclusion: concludes facilitates NCOA4-mediated via ATM–NCOA4 pathway, subsequently liver.

Language: Английский

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Low-dose deoxynivalenol exposure inhibits hepatic mitophagy and hesperidin reverses this phenomenon by activating SIRT1

Journal of Hazardous Materials, Journal Year: 2024, Volume and Issue: 468, P. 133854 - 133854

Published: Feb. 22, 2024

Language: Английский

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Green tea polyphenols inhibit TBBPA-induced lung injury via enhancing antioxidant capacity and modulating the NF-κB pathway in mice

Food & Function, Journal Year: 2024, Volume and Issue: 15(7), P. 3411 - 3419

Published: Jan. 1, 2024

Tetrabromobisphenol A (TBBPA) is a global pollutant. When TBBPA absorbed by the body through various routes, it can have wide range of harmful effects on body. Green tea polyphenols (GTPs) act as antioxidants, resisting toxic animals. The and mechanisms GTP oxidative stress, inflammation apoptosis in mouse lung are unknown. Therefore, we established vivo vitro models exposure antagonism using C57 mice A549 cells examined expression factors related to autophagy, apoptosis. results study showed that increase reactive oxygen species (ROS) levels after decreased autophagy-related Beclin1, LC3-II, ATG3, ATG5, ATG7 ATG12 increased p62; stress inhibits autophagy levels. pro-inflammatory IL-1β, IL-6 TNF-α anti-inflammatory factor IL-10 activation NF-κB p65/TNF-α pathway. Bax, caspase-3, caspase-7 caspase-9 Bcl-2 activate apoptosis-related pathways. addition attenuated levels, restored inhibition reduced Our suggest attenuate modulating ROS, reducing increasing attenuating cells. These provide fundamental information for exploring antioxidant mechanism further studying TBBPA.

Language: Английский

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Hesperidin Alleviates Hepatic Injury Caused by Deoxynivalenol Exposure through Activation of mTOR and AKT/GSK3β/TFEB Pathways

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(25), P. 14349 - 14363

Published: June 13, 2024

Deoxynivalenol (DON) is a common agricultural mycotoxin that chemically stable and not easily removed from cereal foods. When organisms consume food made contaminated crops, it can be hazardous to their health. Numerous studies in recent years have found hesperidin (HDN) has hepatoprotective effects on wide range of toxins. However, few scholars explored the potential HDN attenuating DON-induced liver injury. In this study, we established low-dose DON exposure model intervened with three doses HDN, acting male C57 BL/6 mice AML12 cells, which served as vivo vitro models, respectively, investigate protective mechanism against exposure-induced The results suggested disrupted hepatic autophagic fluxes, thereby impairing structure function, significantly attenuated these changes. Further revealed alleviated excessive autophagy through mTOR pathway lysosomal dysfunction AKT/GSK3β/TFEB pathway. Overall, our study could ameliorate flux disorders via pathway, reducing

Language: Английский

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Synthetic phenolic antioxidants evoked hepatoxicity in grass carp (Ctenopharyngodon idella) through modulating the ROS-PI3K/mTOR/AKT pathway: Apoptosis-autophagy crosstalk

Fish & Shellfish Immunology, Journal Year: 2023, Volume and Issue: 139, P. 108906 - 108906

Published: June 20, 2023

Language: Английский

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Waterborne ammonia toxicity damages crustacean hemocytes via lysosome-dependent autophagy: a case study of swimming crabs Portunus trituberculatus

Environmental Research, Journal Year: 2025, Volume and Issue: unknown, P. 120985 - 120985

Published: Feb. 1, 2025

Language: Английский

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Protective effects of bone marrow mesenchymal stem cell-derived exosomes loaded cerium dioxide nanoparticle against deoxynivalenol-induced liver damage

Journal of Nanobiotechnology, Journal Year: 2025, Volume and Issue: 23(1)

Published: March 17, 2025

Deoxynivalenol (DON), a mycotoxin produced by Fusarium species, posed significant threats to food safety and human health due its widespread prevalence detrimental effects. Upon exposure, the liver, which played crucial role in detoxifying DON, experienced depleted antioxidant levels heightened inflammatory responses. Bone marrow mesenchymal stem cell (BMSC)-derived exosomes (BMSC-exos) exhibited therapeutic potential promoting cellular repair delivering bioactive substances, such as cerium dioxide nanoparticles (CeO₂ NPs), are recognized for their ability mitigate oxidative stress inflammation. We successfully loaded BMSC-exos with CeO2 NPs (BMSC-exos @ CeO2) using extrusion techniques, verified through electron microscopy elemental mapping. The resulting displayed low cytotoxicity, boosted activity, reduced inflammation Hepa 1–6 cells DON condition. In vivo study, maintained stability 72 h, it also can prevent depletion inhibit liver under After treatment, multi-omics analyses further highlighted changes metabolic protein signaling pathways, notably linoleic arachidonic acid metabolism. Key pathways about AMPK JAK1/STAT3 were involved mitigating damage or without DON. Our findings revealed promising strategy against DON's toxicity, offering valuable insights into protection.

Language: Английский

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Protective effects of baicalin against deoxynivalenol-induced oxidative and inflammatory damage in chicken-derived hepatic 3D cell cultures

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: April 1, 2025

Abstract Deoxynivalenol (DON) is a trichothecene mycotoxin often contaminating grains used in poultry feed production and causing several adverse effects farm animals. Therefore, it important to investigate compounds that can be potential candidates mitigate these effects, such as baicalin. The of DON baicalin were investigated chicken-derived 3D hepatic cell cultures, viability, LDH activity, oxidative parameters (NRF-2, 8-OHdG) inflammatory (IL-6, IL-8, IFN-γ) monitored for 24 48 h. Our results suggest reduced cellular metabolic activity but did not prove cytotoxic, was able attenuate this effect. change extracellular suggests after h the cells have already started respond toxin protective mechanisms induced. Based on measured parameters, showed antioxidant longer exposure, our indicate prooxidant Baicalin also had an anti-inflammatory effect based amount IL-6 while exerted dose-and time-dependent pleiotropic activity. These may impact inflammation homeostasis, could alleviate effects.

Language: Английский

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Quercetin alleviates the toxicity of difenoconazole to the respiratory system of carp by reducing ROS accumulation and maintaining mitochondrial dynamic balance

Toxicology and Applied Pharmacology, Journal Year: 2024, Volume and Issue: 484, P. 116860 - 116860

Published: Feb. 10, 2024

Language: Английский

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