Poultry Science,
Journal Year:
2024,
Volume and Issue:
103(5), P. 103637 - 103637
Published: March 8, 2024
To
investigate
the
potential
protective
effect
of
prior
cold
stimulation
on
broiler
intestine
induced
by
acute
stress
(ACS).
A
total
384
one-day-old
broilers
were
divided
into
control
(CON),
ACS,
Ⅰ
(CS3+ACS),
and
Ⅱ
(CS9+ACS)
groups.
Broilers
in
CON
ACS
groups
reared
normally,
birds
CS3+ACS
CS9+ACS
at
3℃
9℃
below
group
for
5
h,
respectively,
alternate
days
from
day
15
to
35.
CS3+ACS,
subjected
10℃
24
h
43.
Eventually,
small
tissues
collected
histopathological
observation
indexes
detection.
The
results
showed
that
intestinal
all
ACS-broilers
exhibited
inflammatory
cell
infiltrates,
microvilli
disruption,
reduced
villus
length
jejunum
increased
crypt
depth
ileum.
Whereas
these
phenomena
relatively
light
group.
Compared
group,
mRNA
expression
TLR4/MyD88/NF-κB
pathway-related
genes
(TLR4,
MyD88,
NF-κBp65,
COX-2,
iNOS,
PTGEs,
TNF-α),
Th1/Th17-derived
cytokines
(IL-1β,
IL-2,
IL-8,
IL-12,
IFN-γ,
IL-17),
HSPs
(HSP40,
HSP60,
HSP70,
HSP90)
was
upregulated
(P
<
0.05),
Th2-deviated
(IL-4,
IL-6,
IL-10,
IL-13)
IκBα
downregulated
0.05)
almost
ACS-broilers.
most
genes,
cytokines,
Th2-derived
0.05).
Protein
levels
TLR4,
p-p65/p65,
p-IκBα/IκBα,
IKK,
TNF-α,
IL-1β,
similar
their
expression.
concentration
sIgA
activities
CAT,
SOD,
GSH-px
decreased
MDA
H2O2
compared
Therefore,
caused
oxidative
inflammation,
leading
gut
immune
dysfunction;
while
mild
normal
rearing
temperature
alleviated
stress-induced
injure
dysfunction
modulating
pathway
broilers.
Poultry Science,
Journal Year:
2024,
Volume and Issue:
103(6), P. 103674 - 103674
Published: March 16, 2024
Microplastics
biological
toxicity,
environmental
persistence
and
chemicals
have
been
paid
widespread
attention.
exposed
to
chicken
spleen
injury
of
the
specific
mechanism
is
unclear.
Thus,
we
randomly
assigned
chickens
4
groups:
C
(normal
diet),
L-MPs
(1
mg/L),
M-MPs
(10
H-MPs
(100
assessed
damage
after
42
d
exposure.
Morphologically,
boundary
between
red
white
pulp
was
blurred,
along
with
expansion
pulp.
It
further
speculated
that
microplastics
induced
mitochondrial
dynamic
homeostasis
(Drp1
upgraded,
Mfn1,
Mfn2,
OPA1
reduced),
provoked
apoptotic
pathway
(Bcl-2/Bax
decreased,
cytc,
caspase3,
caspase9
raised),
resulting
in
redox
imbalance
lipid
peroxide
accumulation
(MDA
increased,
CAT,
GSH,
T-AOC
plummeted),
stimulated
ferroptosis
(FTH1,
GPX4,
SLC7A11
decreased).
Here
explored
impact
polystyrene
on
spleen,
as
well
programmed
death
(apoptosis
ferroptosis)
involved,
regulative
role
mitochondria
this
process.
This
could
be
significant
importance
bridging
gap
laboratory
research
microplastics-induced
chicken.
