Low-cost DIY bioreactor system for educational and research applications: an accessible off-the-shelf bubble column concept DOI Creative Commons
Antoni Gandía,

S. Christiansen

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: April 21, 2025

Abstract The development of efficient, cost-effective bioreactor systems is pivotal in biotechnological processes involving the production microbial products like pharmaceuticals, bio-fuels, and protein-rich biomass. Many models are commercially available all industries; however, prices usually exorbitant, making them inaccessible to low-budget research, schools, start-up companies. This article introduces a low-cost, DIY modular LSF system intended cultivate filamentous fungi other microorganisms following minimalist bubble column approach. Using standard sanitary tri-clamp (TC) fittings common hygienic industries household brewing setups, this assembly offers an affordable, adaptable, reparable, robust solution for at fraction cost commercial alternatives. In addition, wide range budget-friendly sensors controllers can be integrated monitor automate key parameters, these bioreactors equally accessible functional. concept unique its scalability customization possibilities, significantly reducing research prototyping costs.

Language: Английский

Alternative Protein Sources and Novel Foods: Benefits, Food Applications and Safety Issues DOI Open Access
Laura Quintieri, Chiara Nitride, Elisabetta De Angelis

et al.

Nutrients, Journal Year: 2023, Volume and Issue: 15(6), P. 1509 - 1509

Published: March 21, 2023

The increasing size of the human population and shortage highly valuable proteinaceous ingredients has prompted international community to scout for new, sustainable, natural protein resources from invertebrates (e.g., insects) underutilized legume crops, unexploited terrestrial aquatic weeds, fungi. Insect proteins are known their nutritional value, being rich in with a good balance essential amino acids source fatty trace elements. Unconventional crops were found nutritional, phytochemical, therapeutic properties, showing excellent abilities survive extreme environmental conditions. This review evaluates recent state fungi, insects intended as alternative sources, ingredient production incorporation food products, including formulations functional characteristics plant-based edible insect novel foods. Emphasis is also placed on safety issues due presence anti-nutritional factors allergenic and/or legumes. biological activities hydrolysates different sources reviewed, along bioactive peptides displaying antihypertensive, antioxidant, antidiabetic, antimicrobial activity. Due healthy properties these foods high abundance phytochemicals, more consumers expected turn vegetarianism or veganism future, demand such products will be challenge future.

Language: Английский

Citations

45
An insight into the nutritional and medicinal value of edible mushrooms: A natural treasury for human health DOI

Arman Hamza,

Ankit Mylarapu,

K. V. Radha Krishna

et al.

Journal of Biotechnology, Journal Year: 2024, Volume and Issue: 381, P. 86 - 99

Published: Jan. 3, 2024

Language: Английский

Citations

32
Current trends in health-promoting potential and biomaterial applications of edible mushrooms for human wellness DOI

Arman Hamza,

Shreya Ghanekar,

Devarai Santhosh Kumar

et al.

Food Bioscience, Journal Year: 2022, Volume and Issue: 51, P. 102290 - 102290

Published: Dec. 9, 2022

Language: Английский

Citations

42
Fungal mycelium as leather alternative: A sustainable biogenic material for the fashion industry DOI Creative Commons
Ayodeji Amobonye, Japareng Lalung, Mukesh Kumar Awasthi

et al.

Sustainable materials and technologies, Journal Year: 2023, Volume and Issue: 38, P. e00724 - e00724

Published: Sept. 23, 2023

The global leather industry has been at the receiving end of various environmental and ethical backlash as it mainly relies on animal agriculture which contributes to deforestation, greenhouse gas emissions, welfare concerns. In addition, processing hides into generates a huge amount toxic chemicals, ultimately get released environment. Thus, growing concern for sustainability led exploration alternative materials conventional animal- based leather. this regard, application fungal alternatives in material technology is gaining traction because its high biodegradability, biocompatibility, renewability, well affordable carbon-neutral growth processes. Fungal have found possess significant mechanical physical properties, thanks interwoven hyphal network mycelium, antimicrobial activities ascribed their bioactive metabolites. Various species, including those from Agaricus, Fomes, Ganoderma, Phellinus, Pleutorus genera, are currently being investigated potential area. This review, therefore, attempts gain insights recent advances scientific research real-world applications fungal-derived like materials. It makes compelling case sustainable discusses morphology-property relationship mycelium driving innovation. Additionally, current methods major players substitute presented. paper also brings attention challenges facing full deployment substitutes proposes solutions with aim encouraging further resource mobilization acceptance renewable substitute.

Language: Английский

Citations

41
Mycoprotein Production by Submerged Fermentation of the Edible Mushroom Pleurotus ostreatus in a Batch Stirred Tank Bioreactor Using Agro-Industrial Hydrolysate DOI Creative Commons
Georgios Bakratsas,

Angeliki C. Polydera,

Oskar Nilson

et al.

Foods, Journal Year: 2023, Volume and Issue: 12(12), P. 2295 - 2295

Published: June 7, 2023

The demand for cheap, healthy, and sustainable alternative protein sources has turned research interest into microbial proteins. Mycoproteins prevail due to their quite balanced amino acid profile, low carbon footprint high sustainability potential. goal of this was investigate the capability Pleurotus ostreatus metabolize main sugars agro-industrial side streams, such as aspen wood chips hydrolysate, produce high-value with cost. Our results indicate that P. LGAM 1123 could be cultivated both in a C-6 (glucose)- C-5(xylose)-sugar-containing medium mycoprotein production. A mixture glucose xylose found ideal biomass production content rich profile. cultivation 4 L stirred-tank bioreactor using hydrolysate achieved 25.0 ± 3.4 g L−1 production, 1.8 0.4 d−1 specific growth rate yield 54.5 0.5% (g/100 sugars). PCA analysis acids revealed strong correlation between composition produced ratios culture medium. high-nutrient by submerged fermentation edible fungus hydrolysates is promising bioprocess food feed industry.

Language: Английский

Citations

36
A review on the edible mushroom as a source of special flavor: Flavor categories, influencing factors, and challenges DOI Creative Commons
Chunping Jiang, Xiaoyu Duan, Lin Lin

et al.

Food Frontiers, Journal Year: 2023, Volume and Issue: 4(4), P. 1561 - 1577

Published: June 3, 2023

Abstract Edible mushrooms are highly valued for both their culinary and medicinal properties. Unlike traditional condiments, the complex flavor of is not determined by a single component, but rather potential synergistic actions several different components chemical interactions. However, only limited number edible mushroom compounds have been examined in detail, leading to an incomplete understanding overall profile. To address this knowledge gap, it necessary conduct further scientific research on flavors maximize utilization. This review aims provide comprehensive overview substances found mushrooms, including volatile nonvolatile compounds, as well mechanisms which various processing methods affect flavor. Additionally, we will explore factors that hinder development recommend strategies advance technological theoretical these unique valuable food ingredients. In summary, enhancing our profile can better leverage full resource.

Language: Английский

Citations

24
Mushrooms as Functional and Nutritious Food Ingredients for Multiple Applications DOI

Seung Woon You,

Roberta Targino Hoskin, Slavko Komarnytsky

et al.

ACS Food Science & Technology, Journal Year: 2022, Volume and Issue: 2(8), P. 1184 - 1195

Published: June 8, 2022

The interest in mushrooms as functional ingredients has increased the past decade. Mushrooms have low fat content and high fiber protein contents are natural sources of valuable food molecules such ergosterol, polyphenols, terpene terpenoids, mannitol trehalose. been used meat- starch-based formulations with varying degrees success, but their technological performances yet to be investigated fully explored for applications directed emerging alternative, meat-free, clean-label marketplace. Therefore, this review, current scientific data regarding attributes that elicit unique nutritional properties, relevance industry, potential opportunities developing innovative, good-tasting, protein-rich foods from presented discussed.

Language: Английский

Citations

36
Valorization of Agro-Industrial Wastes and Residues through the Production of Bioactive Compounds by Macrofungi in Liquid State Cultures: Growing Circular Economy DOI Creative Commons
Sotirios Pilafidis, Panagiota Diamantopoulou, Konstantinos Gkatzionis

et al.

Applied Sciences, Journal Year: 2022, Volume and Issue: 12(22), P. 11426 - 11426

Published: Nov. 10, 2022

Vast quantities of side streams produced worldwide by the agricultural and food industry present an environmental challenge opportunity for waste upcycling in frame circular bioeconomy. Fungi are capable transforming lignocellulosic residues wastes into a variety added-value compounds with applications functional products, pharmaceuticals, chemicals, enzymes, proteins, emerging sector nutraceuticals. The liquid state culture fungi is efficient potentially scalable reproducible biotechnological tool that allows optimized production fungal metabolites. Particularly, utilization agro-industrial by-products, residues, as substrate macrofungi suggested attainable solution management these streams, contributing to climate change mitigation. This review presents recently published literature field cultures using different substrates, methods, factors affecting their growth metabolite production, well applications, focusing on natural valuable produced.

Language: Английский

Citations

32
Bioactivity of Biomass and Crude Exopolysaccharides Obtained by Controlled Submerged Cultivation of Medicinal Mushroom Trametes versicolor DOI Creative Commons
Galena Angelova, Mariya Brazkova, Dasha Mihaylova

et al.

Journal of Fungi, Journal Year: 2022, Volume and Issue: 8(7), P. 738 - 738

Published: July 17, 2022

The aim of this study is to characterize the bioactivity mycelial biomass and crude exopolysaccharides (EPS) produced by

Language: Английский

Citations

30
Determination of Nutritional and Biochemical Composition of Selected Pleurotus spps. DOI Creative Commons
Asma Irshad,

Aisha Tahir,

Sumaira Sharif

et al.

BioMed Research International, Journal Year: 2023, Volume and Issue: 2023(1)

Published: Jan. 1, 2023

The global demand for good quality food is going to be increased gradually. Mushrooms are broadly used as healthy nutritious meals. nutritional values of extracts from four distinct Pleurotus species-Pleurotus ostreatus, sajor-caju, sapidus, and columbinus-were determined in the current study. Firstly, proximate analysis selected species was performed followed by Bradford assay analyze protein spectrophotometrically; high-performance liquid chromatography (HPLC) sugar determination while GC-MS done determine fatty acids on organic mushrooms. Descriptive statistics were calculate percentages significance SPSS statistics. results depicted that fat, protein, ash, fiber, energy contents, total carbohydrate range 0.64-2.02%, 16.07-25.15%, 2.1-9.14%, 6.21-54.12%, 342.20-394.30 kcal/100 g, 65.66-82.47%, respectively. protein's maximum concentration observed P. ostreatus columbinus>P. sajor-caju>P. sequentially. Various sugars may or not present spps. Among acids, prevalence UFA more than saturated among all From this study, it concluded have excellent composition can valuable a great source biochemical compounds.

Language: Английский

Citations

19