To explore the protective mechanism of promethazine against hippocampal neuron injury based on network pharmacology and experimental verification DOI Creative Commons
Bai Li, Fang Li

Medicine, Journal Year: 2024, Volume and Issue: 103(49), P. e40550 - e40550

Published: Dec. 6, 2024

This study aims to investigate the effect of promethazine (PMZ) on hippocampal neuronal injury through network pharmacology and in vivo experiments. Network pharmacology: The intersection genes PMZ Alzheimer Disease (AD) were obtained, core AD screened. enriched by Gene Ontology Kyoto Encyclopedia Genes Genomes (KEGG) pathway enrichment analyses. In vitro experiment, mouse neurons (HT22) divided into control, glutamate (GLU) model, GLU + treatment groups. control group was given a complete culture medium, model for 24 hours, pretreatment 3 then administered hours. Cell viability determined, cell morphology observed microscopy, reactive oxygen species levels glutathione content detected, protein expression P53, PTGS2, SLC7A11, GPX4 detected western blotting. A total 317 targets, 1934 genes, 125 18 including P53 PTGS2. analysis showed that mainly related proliferation, inflammation, hypoxia, synaptic structure, plasma membrane, oxidoreductase activity. results neuroactive ligand-receptor interaction, senescence, cancer pathway, PI3K-AKT signal neurodegeneration, HIF-1 pathway. experiments: improved GLU-induced decrease morphological changes neurons. inhibited increased injured Up-regulated SLC7A11 expression, regulates SLC7A11-GPX4 antioxidant system protect from oxidative stress injury.

Language: Английский

Synthesis of Naringenin and Senecioic Acid Ester Derivatives and Biological Evaluation of the Astrocyte Antioxidant Mechanism and Reactivity After Inflammatory Stimulus DOI Open Access
Janaína Ribeiro Pereira Soares,

Cleonice Creusa dos Santos,

Lucas Matheus Gonçalves de Oliveira

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(5), P. 2215 - 2215

Published: Feb. 28, 2025

The imbalance between the overproduction of reactive species and antioxidant mechanisms can result in astrogliosis oxidative stress associated with neurodegeneration. Based on described activity naturally occurring flavonoids, this study evaluated flavonoid naringenin senecioic acid ester derivatives cortical astrocytes. Naringenin (S)-naringenin were purified from Citrus paradisi, them 7,4-O-disenecioic naringenin, (S)-7,4-O-disenecioic 7-O-senecioic synthesized tested for by free-radical scavenging reaction DPPH. flavonoids' toxicity glutathione (GS) depletion determined rat astrocyte cultures; effects astrocytes' reactivity was expression glial fibrillary acidic protein (GFAP) measuring nitric oxide (NO) production astrocytes treated lipopolysaccharide (LPS, 1 µg/mL/24 h). compounds (1-10 μM) presented effects, (S)-7,4'-O-disenecioic most effective. (1-100 not toxic to astrocytes, also promoting an effect increasing GSH. Moreover, 7-O-senecioc mitigated induced LPS, reducing GFAP NO production. These findings indicate that present a pharmacological potential as anti-inflammatory brain diseases via modulation response.

Language: Английский

Citations

1
Naringenin alleviates intestinal ischemia/reperfusion injury by inhibiting ferroptosis via targeting YAP/STAT3 signaling axis DOI

Min Hou,

Xiaoxi Li, Feng Chen

et al.

Phytomedicine, Journal Year: 2024, Volume and Issue: 135, P. 156095 - 156095

Published: Sept. 27, 2024

Language: Английский

Citations

5
Naringenin reduces cadmium-induced depression and memory deficits via the remediation of neuroinflammation, glutamate excitotoxicity, and DNA fragmentation DOI Creative Commons
Zehra Batool,

Asia Afzal,

Maha Shahid

et al.

Journal of Hazardous Materials Advances, Journal Year: 2025, Volume and Issue: unknown, P. 100671 - 100671

Published: Feb. 1, 2025

Language: Английский

Citations

0
Advancing nephroprotective strategies: the role of naringenin in preventing gentamicin-induced nephrotoxicity DOI Creative Commons

William Bwambale,

Victor Otu Oka,

Olufunke Onaadepo

et al.

Deleted Journal, Journal Year: 2025, Volume and Issue: 2(1)

Published: April 28, 2025

Language: Английский

Citations

0
Synergistic Dual Responsive Chemo-Hyperthermia Anti-Cancer Treatment of Naringenin encapsulated Nano vehicle for Targeting Non-Small Cell Lung Cancer. DOI
Pavithra Nagarajan, Basma Al‐Najar, Ravi Manoharan

et al.

Journal of Drug Delivery Science and Technology, Journal Year: 2025, Volume and Issue: unknown, P. 106953 - 106953

Published: April 1, 2025

Language: Английский

Citations

0
Naringenin enhances the efficacy of ferroptosis inducers by attenuating aerobic glycolysis by activating the AMPK-PGC1α signalling axis in liver cancer DOI Creative Commons
Yongzhuo Li, Jing Deng, Xiaodong Zhang

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(11), P. e32288 - e32288

Published: June 1, 2024

Liver cancer is a heterogeneous disease characterized by poor responses to standard therapies and therefore unfavourable clinical outcomes. Understanding the characteristics of liver developing novel therapeutic strategies are imperative. Ferroptosis, type programmed cell death induced lipid peroxidation, has emerged as potential target for treatment. Naringenin, natural compound that modulates metabolism targeting AMPK, shows promise in enhancing efficacy ferroptosis inducers. In this study, we utilized lines xenograft mice explore synergistic effects naringenin combination with inducers, examining both phenotypic outcomes molecular mechanisms. Our study results indicate use at non-toxic doses hepatocytes can significantly enhance anticancer inducers (erastin, RSL3, sorafenib). The index method confirmed effect between comparison or alone, combined therapy caused more robust peroxidation hence severe ferroptotic damage cells. inhibition aerobic glycolysis mediated AMPK-PGC1α signalling axis key naringenin's on reducing resistance cancer, cytotoxic cells was reversed after pretreatment an AMPK inhibitor PGC1α inhibitor. Taken together, these findings suggest could boost sensitivity which translational value.

Language: Английский

Citations

3
Potential of Plant-Derived Compounds in Preventing and Reversing Organ Fibrosis and the Underlying Mechanisms DOI Creative Commons
Patrícia dos Santos Azeredo, Daping Fan, E. Angela Murphy

et al.

Cells, Journal Year: 2024, Volume and Issue: 13(5), P. 421 - 421

Published: Feb. 28, 2024

Increased production of extracellular matrix is a necessary response to tissue damage and stress. In normal healing process, the increase in transient. some instances; however, can persist as fibrosis, leading deleterious alterations organ structure, biomechanical properties, function. Indeed, fibrosis now appreciated be an important cause mortality morbidity. Extensive research has illustrated that slowed, arrested or even reversed; few drugs have been approved specifically for anti-fibrotic treatment. This part due complex pathways responsible fibrogenesis undesirable side effects targeting these pathways. Natural products utilized thousands years major component traditional medicine currently account almost one-third used clinically worldwide. A variety plant-derived compounds demonstrated preventative reversal on fibrosis. review will discuss underlying mechanisms identified impact

Language: Английский

Citations

2
Naringenin ameliorates cytotoxic effects of bisphenol A on mouse Sertoli cells by suppressing oxidative stress and modulating mitophagy: An experimental study DOI Creative Commons
Layasadat Khorsandi, Abbas Heidari-Moghadam,

Elham Younesi

et al.

International Journal of Reproductive BioMedicine (IJRM), Journal Year: 2024, Volume and Issue: unknown

Published: May 12, 2024

Background: Bisphenol A (BPA), an endocrine-disrupting agent, is widely used as polycarbonate plastics for producing food containers. BPA exposure at environmentally relevant concentrations can cause reproductive disorders. Objective: The effect of Naringenin (NG) on BPA-induced Sertoli cell toxicity and its mechanism was examined in the present study. Materials Methods: In this experimental-laboratory study, mouse TM4 cells were treated to (0.8 μm) or NG 24 hr 10, 20, 50 μg/ml. Cell viability, reactive oxygen species (ROS) production, malondialdehyde (MDA) content, antioxidant level, mitochondrial membrane potential (MMP) examined. expression mitophagy-related genes, including Parkin PTEN-induced putative kinase 1 (Pink1), also evaluated. Results: significantly lowered viability (p = 0.004). Pink1 levels group increased < 0.001), while MMP considerably decreased 0.001). raised MDA ROS 0.001) reduced biomarkers 0.003). 20 μg/ml could improve 0.034). depending concentration, decrease mRNA protein compared 0.024). enhanced factors, BPA-exposed cells. Conclusion: beneficial impacts are related suppression mitophagy reduction oxidative stress. Key words: Mitophagy, Naringenin, cells, A, Reactive species, Pink1, Parkin.

Language: Английский

Citations

1
Natural Antioxidants: A Comprehensive elucidation of their sources, mechanisms, and applications in Health DOI
Bitwell Chibuye, Indra Sen Singh, Ramasamy Subbaiya

et al.

Next research., Journal Year: 2024, Volume and Issue: unknown, P. 100086 - 100086

Published: Nov. 1, 2024

Language: Английский

Citations

1
To explore the protective mechanism of promethazine against hippocampal neuron injury based on network pharmacology and experimental verification DOI Creative Commons
Bai Li, Fang Li

Medicine, Journal Year: 2024, Volume and Issue: 103(49), P. e40550 - e40550

Published: Dec. 6, 2024

This study aims to investigate the effect of promethazine (PMZ) on hippocampal neuronal injury through network pharmacology and in vivo experiments. Network pharmacology: The intersection genes PMZ Alzheimer Disease (AD) were obtained, core AD screened. enriched by Gene Ontology Kyoto Encyclopedia Genes Genomes (KEGG) pathway enrichment analyses. In vitro experiment, mouse neurons (HT22) divided into control, glutamate (GLU) model, GLU + treatment groups. control group was given a complete culture medium, model for 24 hours, pretreatment 3 then administered hours. Cell viability determined, cell morphology observed microscopy, reactive oxygen species levels glutathione content detected, protein expression P53, PTGS2, SLC7A11, GPX4 detected western blotting. A total 317 targets, 1934 genes, 125 18 including P53 PTGS2. analysis showed that mainly related proliferation, inflammation, hypoxia, synaptic structure, plasma membrane, oxidoreductase activity. results neuroactive ligand-receptor interaction, senescence, cancer pathway, PI3K-AKT signal neurodegeneration, HIF-1 pathway. experiments: improved GLU-induced decrease morphological changes neurons. inhibited increased injured Up-regulated SLC7A11 expression, regulates SLC7A11-GPX4 antioxidant system protect from oxidative stress injury.

Language: Английский

Citations

0