Nontoxic Fluorescent Nanoprobes for Multiplexed Detection and 3D Imaging of Tumor Markers in Breast Cancer DOI Creative Commons
Pavel Sokolov, Galina Nifontova, Pavel Samokhvalov

et al.

Pharmaceutics, Journal Year: 2023, Volume and Issue: 15(3), P. 946 - 946

Published: March 15, 2023

Multiplexed fluorescent immunohistochemical analysis of breast cancer (BC) markers and high-resolution 3D immunofluorescence imaging the tumor its microenvironment not only facilitate making disease prognosis selecting effective anticancer therapy (including photodynamic therapy), but also provides information on signaling metabolic mechanisms carcinogenesis helps in search for new therapeutic targets drugs. The characteristics nanoprobe efficiency, such as sensitivity, target affinity, depth tissue penetration, photostability, are determined by properties their components, fluorophores capture molecules, method conjugation. Regarding individual nanocrystals (NCs) widely used optical vitro vivo, single-domain antibodies (sdAbs) well established highly specific molecules diagnostic applications. Moreover, technologies obtaining functionally active sdAb–NC conjugates with highest possible avidity, all sdAb bound to NC a strictly oriented manner, provide 3D-imaging nanoprobes strong comparative advantages. This review is aimed at highlighting importance an integrated approach BC diagnosis, including detection biomarkers microenvironment, need quantitative profiling mutual location, using advanced approaches thick sections. existing tumors NCs described, main advantages disadvantages nontoxic multiplexed discussed.

Language: Английский

Optical tissue clearing associated with 3D imaging: application in preclinical and clinical studies DOI Creative Commons
Cinzia Brenna, Carolina Simioni, Gabriele Varano

et al.

Histochemistry and Cell Biology, Journal Year: 2022, Volume and Issue: 157(5), P. 497 - 511

Published: March 2, 2022

Abstract Understanding the inner morphology of intact tissues is one most competitive challenges in modern biology. Since beginning twentieth century, optical tissue clearing (OTC) has provided solutions for volumetric imaging, allowing microscopic visualization thick sections tissue, organoids, up to whole organs and organisms (for example, mouse or rat). Recently, also been introduced clinical settings achieve a more accurate diagnosis with support 3D imaging. This review aims give an overview recent developments OTC imaging illustrate their role field medical diagnosis, specific focus on applications. Graphical abstract

Language: Английский

Citations

25
Obesity-Related Neuroinflammation: Magnetic Resonance and Microscopy Imaging of the Brain DOI Open Access

Anita Woo,

Amy Botta,

Sammy Shun Wai Shi

et al.

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(15), P. 8790 - 8790

Published: Aug. 8, 2022

Obesity is a major risk factor of Alzheimer's disease and related dementias. The principal feature dementia loss neurons brain atrophy. mechanistic links between obesity the neurodegenerative processes dementias are not fully understood, but recent research suggests that obesity-related systemic inflammation subsequent neuroinflammation may be involved. Adipose tissues release multiple proinflammatory molecules (fatty acids cytokines) impact blood vessel cells, inducing low-grade can transition to tissues, including brain. Inflammation in brain-neuroinflammation-is one key elements pathobiology disorders; it characterized by activation microglia, resident immune cells brain, structural functional changes other forming parenchyma, neurons. Such cellular have been shown animal models with direct methods, such as confocal microscopy. In humans, less tangible, only indirect methods magnetic resonance (MR) imaging usually used. these studies, associated lower volumes cerebral gray matter, cortex, hippocampus, well altered tissue MR properties (suggesting microstructural variations molecular composition). How human observed using relate seen microscopy understood. This review describes current understanding context obesity-induced inflammation, highlights need for bridge studies.

Language: Английский

Citations

25
Multitargeted interventions to reduce dialysis-induced systemic stress DOI Creative Commons
Bernard Canaud,

Melanie P Stephens,

Milind Nikam

et al.

Clinical Kidney Journal, Journal Year: 2021, Volume and Issue: 14(Supplement_4), P. i72 - i84

Published: Oct. 5, 2021

Hemodialysis (HD) is a life-sustaining therapy as well an intermittent and repetitive stress condition for the patient. In ridding blood of unwanted substances excess fluid from blood, extracorporeal procedure simultaneously induces persistent physiological changes that adversely affect several organs. Dialysis patients experience this systemic usually thrice weekly sometimes more frequently depending on treatment schedule. Dialysis-induced results multifactorial components include schedule (i.e. modality, time), hemodynamic management ultrafiltration, weight loss), intensity solute fluxes, osmotic electrolytic shifts interaction with circuit. Intradialytic morbidity hypovolemia, intradialytic hypotension, hypoxia) clinical expression may act disease modifier, resulting in multiorgan injury long-term morbidity. Thus, while lifesaving, HD exposes patient to stressors, both non-hemodynamic origin. addition, combination cardiocirculatory stress, greatly conditioned by switch hypervolemia hypoxemia electrolyte create pro-arrhythmogenic conditions. Moreover, contact circuit directly activate circulating cells macrophages-monocytes or platelets) protein systems coagulation, complement, phase kallikrein-kinin system), leading induction pro-inflammatory cytokines chronic low-grade inflammation, further contributing poor outcomes. The multifactorial, HD-induced globally reduces tissue perfusion oxygenation could have deleterious consequences functionality vital organs such heart, brain, liver kidney. article, we summarize multisystemic pathophysiological main circulatory factors. Strategies mitigate their effects provide cardioprotective personalized dialytic therapies are proposed reduce burden HD.

Language: Английский

Citations

27
A Review of Ex Vivo X-ray Microfocus Computed Tomography-Based Characterization of the Cardiovascular System DOI Open Access
Lisa Leyssens, Camille Pestiaux, Greet Kerckhofs

et al.

International Journal of Molecular Sciences, Journal Year: 2021, Volume and Issue: 22(6), P. 3263 - 3263

Published: March 23, 2021

Cardiovascular malformations and diseases are common but complex often not yet fully understood. To better understand the effects of structural microstructural changes heart vasculature on their proper functioning, a detailed characterization microstructure is crucial. In vivo imaging approaches noninvasive allow visualizing in 3D. However, spatial image resolution too limited for analyses, hence, ex preferred this purpose. Ex X-ray microfocus computed tomography (microCT) rapidly emerging high-resolution 3D technique used assessment calcified tissues. Contrast-enhanced microCT (CE-CT) or phase-contrast (PC-CT) improve by additionally allowing distinction different low X-ray-absorbing soft review, we present strengths microCT, CE-CT PC-CT quantitative structure and/or heart, substructures healthy diseased state. We also discuss current limitations, mainly with regard to contrasting methods tissue preparation.

Language: Английский

Citations

26
Identification of fish spermatogenic cells through high-throughput immunofluorescence against testis with an antibody set DOI Creative Commons

Ye Ding,

Tao Liu,

Yongming Li

et al.

Frontiers in Endocrinology, Journal Year: 2023, Volume and Issue: 14

Published: April 3, 2023

Image-based identification and quantification of different types spermatogenic cells is great importance, not only for reproductive studies but also genetic breeding. Here, we have developed antibodies against spermatogenesis-related proteins in zebrafish (Danio rerio), including Ddx4, Piwil1, Sycp3, Pcna, a high-throughput method immunofluorescence analysis testicular sections. By testes, our results demonstrate that the expression Ddx4 decreases progressively during spermatogenesis, Piwil1 strongly expressed type A spermatogonia moderately B spermatogonia, Sycp3 has distinct patterns subtypes spermatocytes. Additionally, observed polar Pcna primary spermatocytes at leptotene stage. triple staining types/subtypes were easily characterized. We further demonstrated practicality other fish species, Chinese rare minnow (Gobiocypris rarus), common carp (Cyprinus carpio), blunt snout bream (Megalobrama amblycephala), rice field eel (Monopterus albus) grass (Ctenopharyngodon idella). Finally, proposed an integrated criterion identifying fishes using this approach based on these antibodies. Therefore, study provides simple, practical, efficient tool spermatogenesis species.

Language: Английский

Citations

10
Signal improved ultra-fast light-sheet microscope for large tissue imaging DOI Creative Commons
Md Nasful Huda Prince, Benjamin Garcia, C. Henn

et al.

Communications Engineering, Journal Year: 2024, Volume and Issue: 3(1)

Published: April 2, 2024

Abstract Axially swept light-sheet microscope in conjunction with tissue clearing enables three-dimensional morphological investigation of millimeter-scaled tissues at isotropic sub-micron resolution. However, these microscopes suffer from low detection signal and slow imaging speed. Here we report a simple efficient platform that employs precise control two fixed distant foci for axial sweeping. This full field view 40 frames per second, four-fold improvement over the current state-of-the-art. In addition, particular frame rate, our method doubles compared to existing techniques. To augment overall performance, also developed deep learning based information classifier faster determination boundary. We demonstrated performance on various cleared samples delineated its robustness wide range protocols.

Language: Английский

Citations

3
Bridging high resolution sub-cellular imaging with physiologically relevant engineered tissues DOI

Yasaman Kargar Gaz Kooh,

Nathaniel Huebsch

SSRN Electronic Journal, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

While high-resolution microscopic techniques are crucial for studying cellular structures in cell biology, obtaining such images from thick 3D engineered tissues remains challenging. In this review, we explore advancements fluorescence microscopy, alongside the use of various fluorescent probes and material processing to address these challenges. We navigate through diverse array imaging options available tissue engineering field, wide field super-resolution so researchers can make more informed decisions based on specific interest. Finally, provide some recent examples how traditional limitations sub-cellular architecture within have been overcome by combining with innovative approaches.

Language: Английский

Citations

0
From morphology to single-cell molecules: high-resolution 3D histology in biomedicine DOI Creative Commons
Xintian Xu,

Jimeng Su,

Rongyi Zhu

et al.

Molecular Cancer, Journal Year: 2025, Volume and Issue: 24(1)

Published: March 3, 2025

Language: Английский

Citations

0
Dynamic interactions between cartilaginous and tendinous/ligamentous primordia during musculoskeletal integration DOI Creative Commons
Xinyi Yu,

Ryosuke Kawakami,

Shinsei Yambe

et al.

Development, Journal Year: 2025, Volume and Issue: 152(6)

Published: March 15, 2025

ABSTRACT Proper connections between cartilaginous and muscular primordia through tendinous/ligamentous are essential for musculoskeletal integration. Herein, we report a novel double-reporter mouse model investigating this process via fluorescently visualising scleraxis (Scx) SRY-box containing gene 9 (Sox9) expression. We generated ScxTomato transgenic mice crossed them with Sox9EGFP knock-in to obtain ScxTomato;Sox9EGFP mice. Deep imaging of optically cleared embryos at E13.5 E16.5 revealed previously unknown differences in the dynamic interactions control Scx-deficient Tendon/ligament maturation was evaluated simultaneous detection fluorescence visualisation collagen fibre formation using second harmonic generation imaging. Lack deltoid tuberosity caused misdirected muscle attachment morphological changes. Loss Scx also dysregulated progenitor cell fate determination chondrotendinous junction, resulting rounded enthesis rather than protruding observed control. Hence, our system, combination loss- or gain-of-function approaches, is unique powerful tool that could be used gain comprehensive understanding

Language: Английский

Citations

0
Imaging 3D cell cultures with optical microscopy DOI
Huai-Ching Hsieh,

Qinghua Han,

David Brenes

et al.

Nature Methods, Journal Year: 2025, Volume and Issue: unknown

Published: April 17, 2025

Language: Английский

Citations

0