Scientific Reports,
Journal Year:
2024,
Volume and Issue:
14(1)
Published: Aug. 28, 2024
Lung
adenocarcinoma
(LUAD)
is
the
most
common
subtypes
of
NSCLC.
However,
therapeutic
effects
for
LUAD
are
unsatisfactory
at
current
stage,
so
it
important
to
find
new
molecular
targets
and
strategies.
circRNAs
can
regulate
expression
target
genes
by
binding
microRNAs
(miRNAs)
form
competitive
endogenous
RNAs
(ceRNAs).
Therefore,
we
investigated
functions
circR-4225
in
tumor
progression
its
mechanism
this
paper.
up-regulated
tissues.
EIF4A3,
a
member
eukaryotic
translation
initiation
factor
4A
(EIF4A)
family,
promotes
circR-4225.
acts
as
sponge
down-regulate
miR-507,
which
up-regulation
gene-tumor
necrosis
superfamily
11
(TNFSF11).
Knockdown
cell
lines
inhibit
proliferation
viability,
promote
apoptosis
lines,
be
reverted
inhibiting
miR-507
or
overexpressing
TNFSF11.
To
sum
up,
study
demonstrated
that
was
significantly
tissues,
promoted
sponging
up-regulating
This
provide
early
diagnosis
treatment
LUAD.
New Phytologist,
Journal Year:
2023,
Volume and Issue:
240(2), P. 644 - 662
Published: Aug. 2, 2023
Summary
CircRNAs
exist
widely
in
plants,
but
the
regulatory
mechanisms
for
biogenesis
and
function
of
plant
circRNAs
remain
largely
unknown.
Using
extensive
mutagenesis
expression
plasmids
genetic
transformation
methods,
we
analyzed
anti‐salt
functions
a
new
grape
circRNA
Vv‐circSIZ1
.
We
identified
that
is
mainly
expressed
cytoplasm
xylem.
CircSIZ1
species‐specific,
genomic
circSIZ1‐forming
region
seven
tested
species
could
be
backspliced
Nicotiana
benthamiana
,
not
Arabidopsis
The
retention
length
flanking
introns
was
significantly
positively
correlated
with
its
generation
efficiency.
precise
splicing
does
depend
on
mature
exon
sequence
or
internal
intron
sequences,
AG/GT
signal
sites
branch
site
introns.
spliceosome
activity
inversely
proportional
to
level
Furthermore,
RNA‐binding
proteins
can
regulate
overexpression
improved
salt
tolerance
N
Additionally,
relieve
repressive
effect
VvmiR3631
target
VvVHAc1
also
promoted
transcription
parental
gene.
Overall,
these
results
broaden
our
understanding
plants.
Frontiers in Oncology,
Journal Year:
2023,
Volume and Issue:
13
Published: Oct. 12, 2023
Up
till
the
present
moment,
breast
cancer
is
still
leading
cause
of
cancer-related
death
in
women
worldwide.
Although
treatment
methods
and
protocols
for
are
constantly
improving,
long-term
prognosis
patients
not
optimistic
due
to
complex
heterogeneity
disease,
multi-organ
metastasis,
chemotherapy
radiotherapy
resistance.
As
a
newly
discovered
class
non-coding
RNAs,
ncRNAs
play
an
important
role
various
cancers.
Especially
cancer,
lncRNAs
have
received
extensive
attention
been
confirmed
regulate
progression
through
variety
pathways.
Meanwhile,
study
epigenetic
modification,
including
DNA
methylation,
RNA
methylation
histone
has
developed
rapidly
recent
years,
which
greatly
promoted
RNAs
cancer.
In
this
review,
we
carefully
comprehensively
describe
interactions
between
several
major
classes
modifications
ncRNAs,
as
well
their
different
subsequent
biological
effects,
discuss
potential
practical
clinical
applications.
Food Science & Nutrition,
Journal Year:
2024,
Volume and Issue:
12(4), P. 2523 - 2536
Published: Jan. 4, 2024
Abstract
Allicin
is
a
safe
herbal
extract
believed
to
have
antitumor
effects,
which,
however,
remain
unclear.
The
aim
of
the
present
work
was
discuss
effects
on
cervical
cancer
using
cell
experiments.
Using
Hela
and
Siha
our
research
objectives
in
study,
first
step,
difference
concentration
(20,
40,
80
μM)
treated
lines,
next
circEIF4G2
lines;
proliferation
EdU‐positive
number
by
CCK‐8
EdU
staining;
apoptosis
rate
flow
cytometry;
invasion
transwell
assay;
wound
healing
relative
mRNA
protein
levels
qRT‐PCR
WB
assay.
With
supplement,
were
significantly
depressed
with
increasing;
suppressed
expression
down‐regulation
(
p
<
.05,
respectively).
However,
there
no
significant
among
Allicin,
si‐circEIF4G2,
Allicin+si‐circEIF4G2
biological
activities
including
proliferation,
apoptosis,
migration,
gene
expression.
depresses
cells
through
down‐regulating
circEIF4G2/HOXA1/AKT/mTOR.
Frontiers in Veterinary Science,
Journal Year:
2024,
Volume and Issue:
11
Published: Feb. 7, 2024
Introduction
This
study
evaluated
the
effects
of
Isatis
Leaf
(ISL)
on
growth
performance,
gastrointestinal
tissue
morphology,
rumen
and
intestinal
microbiota,
rumen,
serum
urine
metabolites,
epithelial
transcriptome
fattening
sheep.
Methods
Twelve
3.5-month-old
healthy
sheep
were
randomly
divided
into
two
groups,
each
with
6
replicates,
fed
basal
diet
(CON)
supplemented
80
g/kg
ISL
for
2.5
months.
Gastrointestinal
tract
was
collected
histological
analysis,
fluid
feces
subjected
to
metagenomic
fluid,
serum,
metabolomics
transcriptomics
analysis.
Results
The
results
showed
that
in
group,
average
daily
gain
feed
intake
significantly
lower
than
those
CON
group
(
P
<
0.05),
ammonia
nitrogen
level
higher
0.01).
thickness
reticulum
abomasum
muscle
layer
increased
0.05).
At
genus
level,
addition
modified
composition
fecal
microorganisms,
relative
abundance
Methanobrevibacter
Centipeda
upregulated
Butyrivibrio,
Saccharofermentans,
Mogibacterium,
Pirellula
downregulated
In
Papillibacter,
Pseudoflavonifractor,
Butyricicoccus,
Anaerovorax,
Methanocorpusculum
upregulated,
while
Roseburia,
Coprococcus,
Clostridium
XVIII,
Parasutterella,
Macellibacteroides,
Porphyromonas
There
164,
107,
77
different
metabolites
between
groups
differential
metabolic
pathways
mainly
included
thiamine
metabolism,
niacin
nicotinamide
vitamin
B6
taurine
beta-Alanine
metabolism
riboflavin
metabolism.
These
involved
regulation
energy
immune
function
Transcriptome
sequencing
differentially
expressed
genes
enriched
cellular
physiological
processes,
development,
regulation.
Conclusion
summary,
had
effect
increasing
levels,
regulating
promoting
body
fat
enhancing
immunity
Physiological Reports,
Journal Year:
2024,
Volume and Issue:
12(23)
Published: Nov. 1, 2024
Abstract
Circular
RNAs
have
been
implicated
as
critical
regulators
in
the
initiation
and
progression
of
colorectal
cancer
(CRC).
This
study
was
intended
to
elucidate
functional
significance
circ_0089761/miR‐27b‐3p/programmed
cell
death
ligand
1
(PD‐L1)
axis
CRC.
Our
findings
indicated
that
circ_0089761
expression
significantly
elevated
CRC
tissues
lines.
Furthermore,
high
correlated
with
TNM
stage
tumor
size.
Silencing
inhibited
proliferation,
migration,
invasion,
increased
apoptosis.
Mechanistically,
facilitated
its
biological
function
by
binding
miR‐27b‐3p
upregulate
PD‐L1
Coculture
experiments
confirmed
low
impeded
CD8
+
T
apoptosis
depletion,
activated
function,
secretion
immune
effector
cytokines
IFN‐γ,
TNF‐α,
perforin,
granzyme‐B.
MiR‐27b‐3p
inhibition
or
overexpression
partially
function.
The
circ_0089761/miR‐27b‐3p/PD‐L1
is
postulated
exert
pivotal
functions
mechanistic
it
holds
promising
prospects
a
feasible
biomarker
therapeutic
target
for
Molecular Human Reproduction,
Journal Year:
2023,
Volume and Issue:
29(11)
Published: Oct. 26, 2023
Polycystic
ovary
syndrome
(PCOS)
is
a
common
reproductive
endocrine
disease
that
affects
women
of
age.
It
also
significant
cause
infertility.
Circular
RNAs
have
been
found
to
crucial
role
in
the
development
and
progression
system
diseases.
In
this
study,
we
focused
on
circ_BECN1
aimed
investigate
its
mechanism
PCOS,
providing
foundation
for
early
diagnosis
treatment
condition.
Our
findings
revealed
an
upregulation
expression
ovarian
granulosa
cells
(GCs)
PCOS
patients.
Additionally,
silencing
resulted
inhibited
proliferation
enhanced
apoptosis
human
granulosa-like
tumor
cell
line
(KGN),
therefore
implicating
cycle
process.
Through
dual-luciferase
reporting
assay,
determined
acts
as
sponge
miR-619-5p
Rab5b
target
gene
miR-619-5p.
Moreover,
was
be
upregulated
tissue
Knocking
down
decreased
expression,
which
then
restored
by
using
inhibitor.
rescue
experiments
demonstrated
overexpressing
reversed
effects
knockdown
KGN
cells.
summary,
our
indicate
GCs
promotes
growth
progression,
reduces
modulating
miR-619-5p/Rab5b
axis.
Therefore,
may
serve
potential
therapeutic
treatment.
Journal of Agricultural and Food Chemistry,
Journal Year:
2022,
Volume and Issue:
70(42), P. 13719 - 13729
Published: Oct. 12, 2022
Understanding
more
precisely
the
mechanisms
controlling
metabolism
of
fatty
acid
in
mammary
gland
dairy
goats
is
essential
for
future
improvements
milk
quality.
Particularly
since
recent
data
have
underscored
a
key
role
circular
RNAs
(circRNAs)
function,
high-throughput
sequencing
technology
was
used
to
identify
expression
levels
circRNAs
tissue
during
early
and
peak
lactation
present
study.
Compared
with
lactation,
results
demonstrated
that
level
circ007071
12.02-fold
up-regulated.
Subsequent
studies
goat
epithelial
cells
(GMECs)
revealed
stimulated
synthesis
triglycerides
(TAG)
cholesterol,
as
well
increased
content
saturated
acids
(C16:0
C18:0).
More
importantly,
using
double
luciferase
reporting
system
allowed
us
detect
sequence
at
binding
site
miR-103-5p,
indicating
it
targeted
this
miRNA.
Overexpression
significantly
decreased
abundance
miR-103-5p
led
inhibition
TAG
synthesis.
In
contrast,
peroxisome
proliferator-activated
receptor
γ
(PPARγ),
target
gene
reinforced
overexpression
circ007071.
Thus,
we
conclude
one
function
regulation
fat
attenuate
inhibitory
effect
on
PPARγ
via
direct
interactions
As
result,
process
able
proceed.
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2023,
Volume and Issue:
unknown
Published: Nov. 20, 2023
Abstract
Circular
RNAs
(circRNAs)
are
single-stranded
molecules
that
have
attracted
increasing
attention
in
recent
years
due
to
their
covalently
closed
structure
and
diverse
functional
roles
mammalian
cells,
where
they
involved
the
regulation
of
gene
expression
protein
function.
Increasing
evidence
suggests
circRNAs
similar
functions
plants,
play
a
role
plant
development,
resistance
biotic
stress,
abiotic
stress
tolerance.
Here,
we
investigated
agronomically
relevant
question
whether
synthetic
designer
can
be
used
modulate
sequence-specific
manner
plants.
We
show
treatment
GFP
-expressing
Arabidopsis
protoplasts
with
50
nt
antisense
circRNA
(circRNA
)
reduces
cellular
accumulation
reporter
dose-dependent
manner.
This
inhibitory
activity
was
not
abolished
various
ago
dcl
mutants
defective
RNAi
pathways.
Moreover,
contrast
other
types
RNA
such
as
double-stranded
(ds)RNA,
did
induce
PTI
response
leaves.
discuss
possibility
may
applied
regulate
endogenous
genes
thus
future
potential
novel
bioherbicide.
