Journal of Hazardous Materials Advances, Journal Year: 2025, Volume and Issue: unknown, P. 100635 - 100635
Published: Feb. 1, 2025
Language: Английский
The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 922, P. 171223 - 171223
Published: Feb. 28, 2024
Language: Английский
Citations
6
Water, Journal Year: 2024, Volume and Issue: 16(9), P. 1220 - 1220
Published: April 25, 2024
Background: Wastewater-based epidemiology (WBE) has become crucial for early microbial outbreak detection and public health surveillance globally, underscored by the COVID-19 pandemic. However, despite advancements in sampling analyses, interpreting results estimating infection rates pose challenges. Enhancements sewer system engineering, understanding wastewater environment, addressing impact of environment on accuracy are needed. Objective: This scoping review aims to identify engineering knowledge gaps WBE guide future study designs. Design: Research “wastewater-based epidemiology” involving “engineering”, published between 2015 2023, was extracted from Scopus database. Results: examines elements influencing WBE’s precision reliability, especially identifying measuring SARS-CoV-2 RNA. It identifies significant effects analytical practices, wastewater’s composition performance. Conclusions: calls further investigation into economical evaluation methods these factors enhance data normalization interpretation, utilizing existing treatment plant used control, which could be a cost-effective approach over more expensive population biomarkers. approach, aside SARS-CoV-2, holds potential application broader number types diseases, as well consumption habits.
Language: Английский
Citations
6
Frontiers in Public Health, Journal Year: 2023, Volume and Issue: 11
Published: March 7, 2023
Coronavirus SARS-CoV-2 is a causative agent responsible for the current global pandemic situation known as COVID-19. Clinical manifestations of COVID-19 include wide range symptoms from mild (i.e., cough, fever, dyspnea) to severe pneumonia-like respiratory symptoms. has been demonstrated be detectable in stool patients. Waste-based epidemiology (WBE) shown promising approach early detection and monitoring local population performed via collection, isolation, viral pathogens environmental sources.In order select optimal protocol epidemiological region Turiec, Slovakia, we (1) compared methods separation including virus precipitation by polyethylene glycol (PEG), purification ultrafiltration (Vivaspin®) subsequent isolation NucleoSpin RNA Virus kit (Macherey-Nagel), direct wastewater (Zymo Environ Water Kit); (2) evaluated impact water freezing on SARS- CoV-2 separation, detection; (3) role filtration stability; (4) determined appropriate reverse transcription-droplet digital PCR (RT-ddPCR) real-time quantitative polymerase chain reaction (RT-qPCR) (targeting same genes, i.e., RdRp gene E) samples.(1) Usage Zymo Kit provided superior quality isolated comparison with both ultracentrifugation PEG precipitation. Freezing samples significantly reduces yield. Filtering counterproductive when used. According specificity sensitivity, RT-ddPCR outperforms RT-qPCR.The results our study suggest that WBE valuable warning alert represents non-invasive monitor pathogens, thus protects public health regional national level. In addition, have sensitivity testing nearer limit can improved selecting combination enrichment, methods.
Language: Английский
Citations
14
Water, Journal Year: 2023, Volume and Issue: 15(6), P. 1051 - 1051
Published: March 9, 2023
The analysis of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) gene copy numbers in wastewater samples can provide quantitative information on Disease-19 (COVID-19) cases within a sewer catchment. However, many wastewater-based epidemiology (WBE) studies have neglected virus decay during the transportation process sewers while back-calculating COVID-19 prevalence. Among various condition parameters, temperature and dilution by fresh/saltwater infiltration may result significant change to decay, terms both infectivity Ribonucleic Acid (RNA). This paper reviewed literature identify discuss effects water types (i.e., wastewater, freshwater, seawater) coronavirus based rate constants that were collected from published papers. To evaluate importance sensitivity was then conducted with rates SARS-CoV-2 RNA WBE back-calculation equation. Finally, also compared those other viruses further understand difference among species. found be less impacted variation than viable coronaviruses. Nevertheless, still sensitive increased warm over 26 °C), which could lead two-times higher relative variance estimated prevalence, considering between 4 37 °C catchment 12-h hydraulic retention time. Comparatively, estimation enveloped greater nonenveloped enteric viruses, easily degradable wastewater. In addition, stormwater inflow accompanied cold weather might alleviate infectivity, thus increasing potential risk transmission through Overall, this aims better impact in-sewer processes its implications for WBE. outcome quantitatively inform improve awareness infection via heavy rainfall events. Given identified scarcity data available salt or chemical additions, future research fate subjected dosing treatment plant operations is recommended.
Language: Английский
Citations
13
The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 881, P. 163454 - 163454
Published: April 13, 2023
Wastewater-based epidemiology (WBE) is a promising tool to efficiently monitor COVID-19 prevalence in community. For WBE community surveillance, automation of the viral RNA detection process ideal. In present study, we achieved near full-automation previously established method, COPMAN (COagulation and Proteolysis method using MAgnetic beads for Nucleic acids wastewater), which was then applied detect SARS-CoV-2 wastewater half year. The line employed Maholo LabDroid an automated-pipetting device achieve high-throughput sample-processing capability 576 samples per week. quantified with automated collected from two treatment plants Sagami River basin Japan between 1 November 2021 24 May 2022, when numbers daily reported cases ranged 0 130.3 100,000 inhabitants. detected 81 out 132 at concentrations up 2.8 × 105 copies/L. These showed direct correlations subsequently clinical (5-13 days later), as determined by Pearson's Spearman's cross-correlation analyses. To compare results, also conducted testing EPISENS-S (Efficient Practical virus Identification System ENhanced Sensitivity Solids, Ando et al., 2022), method. correlated only Our shown be efficient timely large-scale monitoring RNA, making more feasible surveillance.
Language: Английский
Citations
13
The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 886, P. 163935 - 163935
Published: May 8, 2023
Coronavirus disease 2019 - caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) -, has triggered a worldwide pandemic resulting in 665 million infections and over 6.5 deaths as of December 15, 2022. The development different epidemiological tools have helped predict new outbreaks assess behavior clinical variables health contexts. In this study, we aimed to monitor concentrations SARS-CoV-2 wastewater tool progression during Waves 3, 5, 6 Spanish city Xátiva from September 2020 March We estimated RNA 195 samples using RT-PCR Diagnostic Panel validated Center for Disease Control Prevention. also compared trends several (14-day cumulative incidence, positive cases, hospital cases stays, critical primary care visits, deaths) each study wave against Pearson's product-moment correlations, two-sided Mann-Whitney U test, cross-correlation analysis. found strong correlations between with 14-day incidence time. Wastewater showed these one two weeks advance. There were significant hospitalizations Wave 3 6; cross-correlations stronger hospitalization stays week before 6. No association vaccination percentages viral was observed. Our findings support potential surveillance anticipate infection data such hospitalizations, stays. Public authorities could use on similar population an aid decision-making epidemic outbreak.
Language: Английский
Citations
13
The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 899, P. 165603 - 165603
Published: July 19, 2023
Language: Английский
Citations
13
Epidemics, Journal Year: 2024, Volume and Issue: 49, P. 100793 - 100793
Published: Sept. 26, 2024
Language: Английский
Citations
5
The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 901, P. 165926 - 165926
Published: July 30, 2023
Hand, foot, and mouth disease (HFMD) is a highly contagious that primarily affects children under five years of age. It mainly caused by serotypes Enterovirus A (EVA): EVA71, Coxsackievirus types 6 (CVA6), 10 (CVA10), 16 (CVA16). Despite being prevalent in Japan other countries the Asia-Pacific region, few studies have investigated HFMD pathogens wastewater. The present study aimed to develop sensitive broadly reactive quantitative polymerase chain reaction (qPCR) assay dominant serotype CVA6, revise previously developed CVA10, CVA16 assays, test these assays wastewater samples from Yamanashi Prefecture, Japan. new-CVA6 qPCR was with maximal nucleotide percent identity among CVA6 isolates revised were had ability quantify respective positive controls at levels as low 1 copy/μL. Among 53 grab influent collected between March 2022 2023, RNA not detected any samples, whereas could detect 38 % (20/53) samples. significantly higher concentration summer season (3.3 ± 0.8 log10 copies/L; 79 (11/14)) than autumn (2.7 0.6 69 (9/13)). seasonal trend detection aligned case reports catchment treatment plant. This first report trends EVA associated provides evidence wastewater-based epidemiology applicable even for diseases are only specific population groups.
Language: Английский
Citations
11
Heliyon, Journal Year: 2024, Volume and Issue: 10(8), P. e29462 - e29462
Published: April 1, 2024
This research evaluated the relationship between daily new Coronavirus Disease 2019 (COVID-19) cases and Severe Acute Respiratory Syndrome 2 (SARS-CoV-2) concentrations in wastewater, followed by effects of differential SARS-CoV-2 shedding loads across various COVID-19 outbreaks. Linear regression analyses were utilized to examine lead time signal wastewater relative clinical cases. During Delta wave, no was evident, highlighting limited predictive capability monitoring during this phase. However, significant times observed Omicron potentially attributed testing capacity overload subsequent case reporting delays or changes patterns. Post-Omicron wave (Febuary 23 May 19, 2022), discernible, whereas following lifting state emergency (May 30, 2022 2023), correlation coefficient increased demonstrated potential surveillance as an early warning system. Subsequently, we explored virus through feces, operationalized ratio varied significantly Delta, Omicron, other variants post-state-emergency phases, with Kruskal-Wallis H test confirming a difference medians these stages (P < 0.0001). Despite its promise, disease prevalence presents several challenges, including variability, data interpretation complexity, impact environmental factors on viral degradation, lack standardized procedures. Overall, our findings offer insights into concentrations, variation different pandemic underscore promise limitations system for trends.
Language: Английский
Citations
4