Low-dose thiram exposure elicits dysregulation of the gut microbial ecology in broiler chickens DOI Creative Commons
Lei Wang, Fazul Nabi,

Weixue Yi

et al.

Ecotoxicology and Environmental Safety, Journal Year: 2023, Volume and Issue: 270, P. 115879 - 115879

Published: Dec. 28, 2023

Thiram, a typical fungicide pesticide, is widely used in agricultural production. The presence of thiram residues not only due to over-utilization, but also primarily attributed long-term accumulation. However, there paucity information regarding the impact prolonged utilization at low doses on gut microbiota, particularly with respect fungi. Our objective explore effect broilers from perspective which includes both bacteria and We developed low-dose model simulate residue employed 16 S rRNA ITS gene sequencing investigate diversity profile microbiota between group CC (normal diet) TC diet supplemented 5 mg/kg thiram). results revealed that had detrimental broiler's growth performance, resulting an approximate reduction 669.33 g their final body weight day 45. findings indicated negative bacterial composition, leading notable abundance Merdibacter, Paenibacillus, Macrococcus, Fournierella, Anaeroplasma (p < 0.05) compared group. Conversely, relative level Myroides was significantly increased response exposure. In fungi, enhanced richness fungal populations 0.05), as evidenced by increase alpha indices, i.e. ACE (CC: 346.49 ± 117.27 vs TC: 787.27 379.14, p Chao 1 317.63 69.13 504.85 104.50, Shannon 1.28 1.19 5.39 2.66, Simpson 0.21 0.78 0.34, 0.05). Furthermore, Ascomycota, Kickxellomycota, Glomeromycota were exposure thiram, conversely, Basidiomycota decreased Overall, this study demonstrated induced significant changes composition broilers, more pronounced observed community community. Importantly, our further emphasize potential risks associated dose have novel discovery indicating alterations fungi may serve crucial factor contributing effects exerted residues.

Language: Английский

Self-assembled C-Ag hybrid nanoparticle on nanoporous GaN enabled ultra-high enhancement factor SERS sensor for sensitive thiram detection DOI
Hongpeng Zhou, Jiaoyan Qiu, Yunhong Zhang

et al.

Journal of Hazardous Materials, Journal Year: 2024, Volume and Issue: 469, P. 133868 - 133868

Published: Feb. 24, 2024

Language: Английский

Citations

27
The autophagy-mediated mechanism via TSC1/mTOR signaling pathway in thiram-induced tibial dyschondroplasia of broilers DOI

Chuxian Quan,

Shimeng Zhou,

Yan Zhang

et al.

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 928, P. 172305 - 172305

Published: April 7, 2024

Language: Английский

Citations

5
Preparation of cellulose-based flexible SERS and its application for rapid and ultra-sensitive detection of thiram on fruits and vegetables DOI
Haonan Wang, Yujia Chen,

Yixuan Yang

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 262, P. 129941 - 129941

Published: Feb. 9, 2024

Language: Английский

Citations

4
Slide‐Ring Structured Stress‐Electric Coupling Hydrogel Microspheres for Low‐Loss Transduction Between Tissues DOI Open Access
Fan Wang, Xiaoyu Han, Zeyu Han

et al.

Advanced Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 2, 2025

High transductive loss at tissue injury sites impedes repair. The high dissipation characteristics in the electromechanical conversion of piezoelectric biomaterials pose a challenge. Therefore, supramolecular engineering and microfluidic technology is utilized to introduce slide-ring polyrotaxane conductive polypyrrole construct stress-electric coupling hydrogel microspheres. molecular slippage mechanism structure stores releases mechanical energy, reducing loss, barium titanate enables stress-electricity conversion, conjugated π-electron movement network improves internal electron transfer efficiency microspheres, thereby for first time. Compared traditional low-dissipation microspheres increased by 2.3 times, energy decreased 43%. At cellular level, electrical signals generated triggered Ca

Language: Английский

Citations

0
Thiram-induced ER stress promotes mitochondrial calcium signaling and NLRP3 inflammasome activation in a tissue specific manner DOI Creative Commons
Shah Nawaz, Muhammad Fakhar‐e‐Alam Kulyar, Quan Mo

et al.

Ecotoxicology and Environmental Safety, Journal Year: 2025, Volume and Issue: 293, P. 118026 - 118026

Published: March 1, 2025

Thiram, a broadly used dithiocarbamate fungicide, exaggerates endoplasmic reticulum (ER) stress and interferes with mitochondrial function, thus disrupting cellular homeostasis. Here, we intend to identify the molecular actions of thiram at mitochondrial-associated ER membranes (MAMs) that lead induction calcium overload in both liver bone tissues. Taken together, show thiram-induced remodelling MAMs leads huge dysregulation. Histological immunohistochemical examinations revealed hyperactivation IP3R1 mediated release calcium, but uptake was by voltage-dependent anion channels VDAC1. This response characterized increased glucose regulated protein 78 (GRP78) expression tibial growth plates (GP). In this respect, new liver-bone axis delineated for stress. More interestingly, activation NLRP3 inflammasome very striking not Hence, results highlight systemic effects identifying critical metabolic junction might play role disorders such as dyschondroplasia related disorders, e.g., osteoarthritis osteoporosis.

Language: Английский

Citations

0
miR-181b-1-3p affects the proliferation and differentiation of chondrocytes in TD broilers through the WIF1/Wnt/β-catenin pathway DOI

Qiuyu Sun,

Shouyan Wu, Kai Liu

et al.

Pesticide Biochemistry and Physiology, Journal Year: 2023, Volume and Issue: 197, P. 105649 - 105649

Published: Oct. 15, 2023

Language: Английский

Citations

10
miR-206a-3p suppresses the proliferation and differentiation of chicken chondrocytes in tibial dyschondroplasia by targeting BMP6 DOI Creative Commons

Yuru Jiang,

Yuxiang Lu, Hengyong Xu

et al.

Poultry Science, Journal Year: 2024, Volume and Issue: 103(4), P. 103534 - 103534

Published: Feb. 7, 2024

The poultry skeletal system serves multiple functions, not only providing structural integrity but also maintaining the balance of essential minerals such as calcium and phosphorus. However, in recent years, consideration traits has been overlooked selective breeding broilers, resulting an inadequate adaptation to cope with rapid increase body weight. Consequently, this leads lameness bone diseases tibial dyschondroplasia (TD), which significantly impact production performance broilers. Accumulating evidence shown that microRNAs (miRNA) play a crucial role differentiation, formation, disease cartilage. miRNA-mediated molecular mechanism underlying chicken TD formation is still poorly understood. objective study was investigate biological function regulatory miRNA formation. Based on transcriptome sequencing cartilage healthy group group, miR-206a-3p found be highly expressed explored through transfection test mimics inhibitor. In study, we utilized qRT-PCR, CCK-8, EdU, western blot, flow cytometry detect proliferation, apoptosis chondrocytes. results revealed suppressed proliferation differentiation chondrocytes while promoting their programmed cell death. Furthermore, biosynthesis dual luciferase assays, it determined BMP6 direct target gene miR-206a-3p. This finding further supported by rescue experiments confirmed involvement pathway governed Our suggest can inhibits promote BMP-6 suppressing Smad2/3 signaling

Language: Английский

Citations

3
Bacillus amyloliquefaciens attenuates the intestinal permeability, oxidative stress and endoplasmic reticulum stress: transcriptome and microbiome analyses in weaned piglets DOI Creative Commons

Junmeng Yuan,

Hongling Meng,

Yu Liu

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: May 13, 2024

Endoplasmic reticulum (ER) stress is related to oxidative (OS) and leads intestinal injury. Bacillus amyloliquefaciens SC06 (SC06) can regulate OS, but its roles in ER remains unclear. Using a 2 × factorial design, 32 weaned piglets were treated by two levels (0 or 1 10 8 CFU/g), either with without diquat (DQ) injection. We found that increased growth performance, decreased ileal permeability, OS DQ-treated piglets. Transcriptome showed differentially expressed genes (DEGs) induced DQ enriched NF-κB signaling pathway. DEGs between DQ- + glutathione metabolism Ileal microbiome revealed the treatment Clostridium Actinobacillus . Correlations microbiota genes. In conclusion, dietary supplementation regulating microbiota.

Language: Английский

Citations

3
Molecular mechanisms of environmental pollutant-induced cartilage damage: from developmental disorders to osteoarthritis DOI
Anatoly V. Skalny, Michael Aschner, Feng Zhang

et al.

Archives of Toxicology, Journal Year: 2024, Volume and Issue: 98(9), P. 2763 - 2796

Published: May 17, 2024

Language: Английский

Citations

3
G-site residue S67 is involved in the fungicide-degrading activity of a tau class glutathione S-transferase from Carica papaya DOI Creative Commons

Suyan Wang,

Yanxia Wang,

Sheng-Shuo Yue

et al.

Journal of Biological Chemistry, Journal Year: 2024, Volume and Issue: 300(4), P. 107123 - 107123

Published: Feb. 28, 2024

Thiram is a toxic fungicide extensively used for the management of pathogens in fruits. Although it known that thiram degrades plant tissues, key enzymes involved this process remain unexplored. In study, we report tau class glutathione S-transferase from Carica papaya (TCpGST) can degrade thiram. This enzyme was easily obtained by heterologous expression E. coli, showed low promiscuity towards other thiuram disulfides, and catalyzed degradation under physiological reaction conditions. Site-directed mutagenesis indicated G-site residue S67 shows influence enzymatic activity thiram, while mutation S13, which reduced oxidase activity, did not significantly affect thiram-degrading activity. The formation dimethyl dithiocarbamate, subsequently converted into carbon disulfide, dithiocarbamoylsulfenic acid as products suggested undergoes an alkaline hydrolysis involves rupture disulfide bond. Application GST selective inhibitor 4-chloro-7-nitro-2,1,3-benzoxadiazole (NBD-Cl) peel 95%, indicating main route papaya. TCpGST also fungicides chlorothalonil thiabendazole, with showing again These results fill important knowledge gap understanding catalytic GSTs reveal new insights fate

Language: Английский

Citations

2