Techniques for immobilizing enzymes to create durable and effective biocatalysts

Results in Chemistry, Journal Year: 2024, Volume and Issue: 7, P. 101486 - 101486

Published: Jan. 1, 2024

This comprehensive review focuses on strategies for creating efficient and robust immobilized biocatalysts. It explores various physicochemical approaches that hold promise enzyme stabilization enhancement of catalytic potential. One approach involves designing permeable scaffolds, emphasizing the importance support structure ability to fine-tune biocatalyst activity. Another pathway is establishing covalent bonds between enzymes surfaces, anchoring biocatalysts longevity sustained activity in industrial research applications. Polymers are also used modify formulation enzymes, enhancing stability providing a versatile platform biocatalytic reactions. These particularly valuable when within pre-established scaffolds. They enable researchers engineers utilize biocatalysis diverse applications, such as biopharmaceutical production sustainable chemical synthesis. Furthermore, these fixed-stabilized derivatives offer significant advancements reaction optimization reactor design. The reported enhancements remarkable, with performance gains ranging from 500 an impressive 20,000-fold compared diluted soluble enzymes. In summary, this highlights innovative developing presents exciting avenues industries seeking harness potential not only enhanced efficiency but contribute environmentally friendly processes.

Language: Английский

---

Pros and Cons in Various Immobilization Techniques and Carriers for Enzymes

Applied Biochemistry and Biotechnology, Journal Year: 2024, Volume and Issue: 196(9), P. 5633 - 5655

Published: Jan. 4, 2024

Language: Английский

---

Enzyme Immobilization using Covalent Organic Frameworks: From Synthetic Strategy to COFs Functional Role

ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: 16(31), P. 40371 - 40390

Published: July 29, 2024

Enzymes, a class of biocatalysts, exhibit remarkable catalytic efficiency, specificity, and selectivity, governing many reactions that are essential for various cascades within living cells. The immobilization structurally flexible enzymes on appropriate supports holds significant importance in facilitating biomimetic transformations extracellular environments. Covalent organic frameworks (COFs) have emerged as ideal candidates enzyme due to high surface tunability, diverse chemical/structural designs, exceptional stability, metal-free nature. Various techniques been proposed fabricate COF-enzyme biocomposites, offering enhancements activity reusability COF-immobilized well new insights into developing advanced enzyme-based applications. In this review, we provide comprehensive overview state-of-the-art strategies immobilizing COFs by focusing their applicability versatility. These systematically summarized compared categorizing them postsynthesis situ immobilization, where respective strengths limitations thoroughly discussed. Combined with an critical emerging applications, further elucidate the multifaceted roles subsequent highlighting biofunctionality achievable through COFs.

Language: Английский

---

Decorating Cage-Shaped Cavities with Carboxyl Groups on Two-Dimensional MOF Nanosheet for Trace Uranium(VI) Trapping

Inorganic Chemistry, Journal Year: 2024, Volume and Issue: 63(32), P. 15105 - 15114

Published: July 31, 2024

The efficient and complete extraction of uranium from aqueous solutions is crucial for safeguarding human health potential radiotoxicity chemotoxicity. Herein, an ultrathin 2D metal-organic framework (MOF) nanosheet with cavity structures was elaborately constructed, based on a calix[4]arene ligand. large molecular skeleton cup-shaped feature the enabled as-prepared MOFs layer separations, which can be readily delaminated into single-layer (∼1.25 nm) nanosheets. incorporation permanent to MOF nanosheets fully utilize their structural features accessible adsorption groups exposed surface area in removal, reaching ultrafast kinetics; functionalized structure endowed ability preconcentrate extract ultrahigh efficiencies, even at extremely low concentrations. As result, relatively high removal ratios (>95%) achieved within 5 min, ultralow concentration range 75-250 ppb, residual reduced below 4.9 ppb. also exhibited anti-interference ability, could efficiently remove low-level (∼150 ppb) various real samples. characterizations density functional theory calculations demonstrated that synergistic effects multiple interactions between carboxylate cage-like cavities uranyl ions responsible selective extraction.

Language: Английский

---

Covalent organic framework-immobilized enzymes: A robust engineered catalytic platform for diverse applications

Nano Energy, Journal Year: 2025, Volume and Issue: unknown, P. 110682 - 110682

Published: Jan. 1, 2025

Language: Английский

---

Lipase in-situ immobilized in covalent organic framework: Enzymatic properties and application in the preparation of 1, 3-dioleoyl-2-palmitoylglycerol

Colloids and Surfaces B Biointerfaces, Journal Year: 2024, Volume and Issue: 238, P. 113873 - 113873

Published: March 23, 2024

Language: Английский

---

Frontiers in design and applications of biomacromolecule@COFs composites

Coordination Chemistry Reviews, Journal Year: 2024, Volume and Issue: 515, P. 215965 - 215965

Published: May 23, 2024

Language: Английский

---

A Review of the Strategic Use of Sodium Alginate Polymer in the Immobilization of Microorganisms for Water Recycling

Polymers, Journal Year: 2024, Volume and Issue: 16(6), P. 788 - 788

Published: March 12, 2024

In the quest for advanced and environmentally friendly solutions to address challenges in field of wastewater treatment, use polymers such as sodium alginate (Na-Alg) combination with immobilized microorganisms (IMs) stands out a promising strategy. This study assesses potential Na-Alg immobilizing emphasizing its effectiveness relevance environmental preservation through IMs. Advances IMs are examined, interactions between these immobilization support highlighted. Additionally, models studying kinetic degradation contaminants importance oxygen supply detailed. The shows promise context improving water quality, preserving ecological balance, addressing climate change, but further research is required overcome identified challenges. Additional areas explore discussed, which expected contribute innovation relevant systems.

Language: Английский

---

Enzyme-Immobilized Porous Crystals for Environmental Applications

Environmental Science & Technology, Journal Year: 2024, Volume and Issue: 58(27), P. 11869 - 11886

Published: June 28, 2024

Developing efficient technologies to eliminate or degrade contaminants is paramount for environmental protection. Biocatalytic decontamination offers distinct advantages in terms of selectivity and efficiency; however, it still remains challenging when applied complex matrices. The main challenge originates from the instability difficult-to-separate attributes fragile enzymes, which also results issues compromised activity, poor reusability, low cost-effectiveness, etc. One viable solution harness biocatalysis environments known as enzyme immobilization, where a flexible tightly fixed solid carrier. In case reticular crystal utilized support, feasible engineer next-generation biohybrid catalysts functional complicated media. This can be interpreted by three aspects: (1) highly crystalline skeleton shield immobilized against external stressors. (2) porous network ensures high accessibility interior catalytic decontamination. And (3) adjustable unambiguous structure framework favors in-depth understanding interfacial interaction between enzyme, turn guide us designing active biocomposites. Review aims introduce this emerging technology involving pollutant degradation greenhouse gas (carbon dioxide) conversion, with emphasis on immobilization protocols diverse catalysis principles including single catalysis, cascades, photoenzyme-coupled catalysis. Additionally, remaining challenges forward-looking directions field are discussed. We believe that may offer useful biocatalytic contribute green sustainable manner will inspire more researchers at intersection environment science, biochemistry, materials science communities co-solve problems.

Language: Английский

---

Covalent Organic Framework‐derived Composite Membranes for Water Treatment

Chemistry - An Asian Journal, Journal Year: 2023, Volume and Issue: 19(2)

Published: Dec. 11, 2023

Abstract Water treatment has experienced a surge in the adoption of membrane separation technology. Covalent organic frameworks (COFs), class metal‐free and open‐framework materials, have emerged as potential materials owing to their interconnected periodic porosity, tunability, chemical stability. However, challenges associated with processing COF powders into self‐standing membranes spurred emergence composite membranes. This review article highlights rationale behind developing categories, including mixed matrix (MMMs) thin film (TFC) The common fabrication techniques each category are presented. In addition, influence additives on performance resultant is systematically discussed, focus recent progress applying different categories water pollutants, ions/molecules, toxic solvents, proteins, heavy metals, radionuclides.

Language: Английский

---