Development of a specific MPXV antigen detection immunodiagnostic assay DOI Creative Commons
Ian Davis,

Jackie M. Payne,

Victoria L. Olguin

et al.

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: Sept. 7, 2023

Human monkeypox (mpox) has recently become a global public health emergency; however, assays that detect mpox infection are not widely available, largely due to cross-reactivity within the Orthopoxvirus genus. Immunoassay development was confined researchers who focus on biothreats and endemic areas (Central West Africa) until 2022 outbreak. As noted in COVID-19 pandemic, antigen detection assays, integrated with molecular necessary help curb spread of disease. Antigen-detecting immunoassays offer advantage providing results ranging from min h lateral flow formats; they can be deployed for point-of-care, home, or field use. This study reports an mpox-specific immunoassay developed multiplexed, magnetic-bead-based platform utilizing reagents all research sectors (commercial, academic, governmental). Two semi-quantitative were parallel standardized infectious virus (MPXV) cell culture fluid MPXV-positive non-human primate (NHP) sera samples. These could viral antigens serum, highly specific toward MPXV as compared other orthopoxviruses (vaccinia virus, cowpox camelpox virus), exhibited correlation quantitative PCR NHP study. Access toolbox will key identifying cases ensuring proper treatment, is currently traveler.

Language: Английский

Progress and prospects on vaccine development against monkeypox infection DOI Open Access
Mohamed J. Saadh, Tahmineh Ghadimkhani, Narges Soltani

et al.

Microbial Pathogenesis, Journal Year: 2023, Volume and Issue: 180, P. 106156 - 106156

Published: May 17, 2023

Language: Английский

Citations

45
Mpox: An Overview of Pathogenesis, Diagnosis, and Public Health Implications DOI Open Access
Francesco Branda, Chiara Romano, Massimo Ciccozzi

et al.

Journal of Clinical Medicine, Journal Year: 2024, Volume and Issue: 13(8), P. 2234 - 2234

Published: April 12, 2024

Mpox, caused by viruses of the genus Orthopoxvirus, is an emerging threat to human and animal health. With increasing urbanization more frequent interaction between humans wild animals, risk Mpox transmission has increased significantly. This review aims examine in depth epidemiology, pathogenesis, diagnosis with a special focus on recent discoveries advances understanding disease. Molecular mechanisms involved viral replication will be examined, as well factors associated interspecific spread disease populations. Currently available diagnostic methods also discussed, critical analysis their limitations possible future directions for improving accuracy timeliness diagnosis. Finally, this explore public health implications emphasizing importance epidemiological surveillance, vaccination, emergency preparedness prevent manage outbreaks. Understanding epidemiology control strategies protecting communities mitigating

Language: Английский

Citations

28
Exploring monkeypox virus proteins and rapid detection techniques DOI Creative Commons

Kamila Sagdat,

Assel Batyrkhan,

Damira Kanayeva

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2024, Volume and Issue: 14

Published: May 28, 2024

Monkeypox (mpox) is an infectious disease caused by the mpox virus and can potentially lead to fatal outcomes. It resembles infections viruses from other families, challenging identification. The pathogenesis, transmission, clinical manifestations of Orthopoxvirus species are similar due their closely related genetic material. This review provides a comprehensive discussion roles various proteins, including extracellular enveloped (EEV), intracellular mature (IMV), profilin-like proteins mpox. also highlights recent diagnostic techniques based on these detect this infection rapidly.

Language: Английский

Citations

19
Unveiling the Global Surge of Mpox (Monkeypox): A comprehensive review of current evidence DOI Creative Commons
Phelipe Magalhães Duarte, Ridwan Olamilekan Adesola, Swagatika Priyadarsini

et al.

The Microbe, Journal Year: 2024, Volume and Issue: 4, P. 100141 - 100141

Published: Aug. 17, 2024

Monkeypox, now named mpox, has emerged as a significant public threat, evidenced by the 2022 outbreak affecting over seventy countries globally. This infectious disease presents debilitating symptoms, including painful skin rashes, mucosal lesions, enlarged lymph nodes, and fever. The situation intensifies with concerns about novel transmission route, specifically through sexual contact, evolution of more transmissible strains. Complicating matters further is documented spillback mpox from humans to animals, raising potential for new animal reservoirs. study utilized systematic approach gather, analyse, interpret data regarding global outbreaks, phylogenomics, human APOBEC3 enzyme activity, antiviral resistance issues, application One Health intervention. Emphasizing covers various aspects, zoonotic origins, pathogenesis, changing epidemiological landscapes, phylogenomic diversity, clade dynamics. review underscores crucial role collaboration in understanding combatting making it valuable resource shaping effective prevention control measures on scale.

Language: Английский

Citations

19
Human monkeypox virus: Epidemiologic review and research progress in diagnosis and treatment DOI
Yanhong Sun,

Wenjian Nie,

Dandan Tian

et al.

Journal of Clinical Virology, Journal Year: 2024, Volume and Issue: 171, P. 105662 - 105662

Published: Feb. 28, 2024

Language: Английский

Citations

17
Human monkeypox: history, presentations, transmission, epidemiology, diagnosis, treatment, and prevention DOI Creative Commons
Mahdi Zahmatyar, Asra Fazlollahi,

Alireza Motamedi

et al.

Frontiers in Medicine, Journal Year: 2023, Volume and Issue: 10

Published: July 20, 2023

Human monkeypox is a zoonotic infection that similar to the diseases caused by other poxviruses. It endemic among wild rodents in rainforests of Central and Western Africa, can be transmitted via direct skin contact or mucosal exposure infected animals. The initial symptoms include fever, headache, myalgia, fatigue, lymphadenopathy, last which main symptom distinguishes it from smallpox. In order prevent manage disease, those who are must rapidly diagnosed isolated. Several vaccines have already been developed (e.g., JYNNEOS, ACAM2000 ACAM3000) antiviral drugs cidofovir tecovirimat) also used treat disease. present study, we reviewed history, morphology, clinical presentations, transmission routes, diagnosis, prevention, potential treatment strategies for monkeypox, enable health authorities physicians better deal with this emerging crisis.

Language: Английский

Citations

37
Immunogenic proteins and potential delivery platforms for mpox virus vaccine development: A rapid review DOI Open Access
Yang Wang, Kaiwen Yang,

Hao Zhou

et al.

International Journal of Biological Macromolecules, Journal Year: 2023, Volume and Issue: 245, P. 125515 - 125515

Published: June 21, 2023

Language: Английский

Citations

28
Genomic and transcriptomic analysis of the recent Mpox outbreak DOI Creative Commons
Federico M. Giorgi, Daniele Pozzobon, Antonio Di Meglio

et al.

Vaccine, Journal Year: 2024, Volume and Issue: 42(7), P. 1841 - 1849

Published: Feb. 3, 2024

The Mpox (formerly named Monkeypox) virus is the etiological cause of a recent multi-country outbreak, with thousands distinct cases detected outside endemic areas Africa as December 2023. In this article, we analyze sequences full genomes from Europe and compare them all available historical relevance, annotated by year geographic origin, well related Cowpox Variola (smallpox) sequences. Our results show that outbreak most likely originating West African clade Mpox, >99 % sequence identity derived cases, dating 1971 to 2017. We specific mutations occurring in viral proteins between current previous sequences, virus. Genome-wide analysis other Mpox/Cowpox/Variola viruses shows very high conservation, 97.9 (protein-based) 97.8 (nucleotide-based) identity. identified significant correlation human transcriptional responses well, conserved immune pathway response induced cell cultures three families Pox similarities major strains viruses, within clades, both at genomic transcriptomic levels, provide molecular basis for observed efficacy vaccines Poxviruses.

Language: Английский

Citations

14
The monkeypox virus-host interplays DOI Creative Commons

Xuemei Yi,

Yali Lei,

Mi Li

et al.

Cell Insight, Journal Year: 2024, Volume and Issue: 3(5), P. 100185 - 100185

Published: July 14, 2024

Monkeypox virus (MPXV) is a DNA belonging to the

Language: Английский

Citations

14
Transmission dynamics, complications and mitigation strategies of the current mpox outbreak: A comprehensive review with bibliometric study DOI
Ranjan K. Mohapatra, Puneet Kumar Singh, Francesco Branda

et al.

Reviews in Medical Virology, Journal Year: 2024, Volume and Issue: 34(3)

Published: May 1, 2024

Abstract As the mankind counters ongoing COVID‐19 pandemic by novel severe acute respiratory syndrome coronavirus‐2 (SARS‐CoV‐2), it simultaneously witnesses emergence of mpox virus (MPXV) that signals at global spread and could potentially lead to another pandemic. Although MPXV has existed for more than 50 years now with most human cases being reported from endemic West Central African regions, disease is recently in non‐endemic regions too affect countries. Controlling important due its potential danger a spread, causing morbidity mortality. The article highlights transmission dynamics, zoonosis potential, complication mitigation strategies infection, concludes suggested ‘one health’ approach better management, control prevention. Bibliometric analyses data extend understanding provide leads on research trends, need revamp critical healthcare interventions. Globally published mpox‐related literature does not align well areas/regions occurrence which should ideally have been scenario. Such demographic geographic gaps between location work epicentres be bridged greater effective translation outputs pubic systems, suggested.

Language: Английский

Citations

11