Chronic wounds

Nature Reviews Disease Primers, Journal Year: 2022, Volume and Issue: 8(1)

Published: July 21, 2022

Language: Английский

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Bacillus subtilis biofilm formation and social interactions

Nature Reviews Microbiology, Journal Year: 2021, Volume and Issue: 19(9), P. 600 - 614

Published: April 6, 2021

Language: Английский

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Bacillus subtilis: a universal cell factory for industry, agriculture, biomaterials and medicine

Microbial Cell Factories, Journal Year: 2020, Volume and Issue: 19(1)

Published: Sept. 3, 2020

Due to its clear inherited backgrounds as well simple and diverse genetic manipulation systems, Bacillus subtilis is the key Gram-positive model bacterium for studies on physiology metabolism. Furthermore, due highly efficient protein secretion system adaptable metabolism, it has been widely used a cell factory microbial production of chemicals, enzymes, antimicrobial materials industry, agriculture, medicine. In this mini-review, we first summarize basic tools expression systems bacterium, including traditional methods novel engineering systems. Secondly, briefly introduce applications in chemicals advantages, mainly focusing some noteworthy products recent progress B. subtilis. Finally, review also covers such additives antimicrobials, biofilm spore formation. We hope provide an overview novice researchers area, offering them better understanding applications.

Language: Английский

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Enhanced surface colonisation and competition during bacterial adaptation to a fungus

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: May 27, 2024

Abstract Bacterial-fungal interactions influence microbial community performance of most ecosystems and elicit specific behaviours, including stimulating specialised metabolite production. Here, we use a co-culture experimental evolution approach to investigate bacterial adaptation the presence fungus, using simple model bacterial-fungal encompassing bacterium Bacillus subtilis fungus Aspergillus niger . We find in one evolving population that B. was selected for enhanced production lipopeptide surfactin accelerated surface spreading ability, leading inhibition fungal expansion acidification environment. These phenotypes were explained by mutations DegS-DegU two-component system. In surfactin, hyphae exhibited bulging cells with delocalised secretory vesicles possibly provoking an RlmA-dependent cell wall stress. Thus, our results indicate selects increased production, which inhibits growth facilitates competitive success bacterium.

Language: Английский

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Water Conservation and Plant Survival Strategies of Rhizobacteria under Drought Stress

Agronomy, Journal Year: 2020, Volume and Issue: 10(11), P. 1683 - 1683

Published: Oct. 30, 2020

Drylands are stressful environment for plants growth and production. Plant growth-promoting rhizobacteria (PGPR) acts as a rampart against the adverse impacts of drought stress in drylands enhances plant is helpful agricultural sustainability. PGPR improves tolerance by implicating physio-chemical modifications called rhizobacterial-induced endurance resilience (RIDER). The RIDER response includes; alterations phytohormonal levels, metabolic adjustments, production bacterial exopolysaccharides (EPS), biofilm formation, antioxidant resistance, including accumulation many suitable organic solutes such carbohydrates, amino acids, polyamines. Modulation moisture status these PGPRs one primary mechanisms regulating growth, but studies on their effect survival scarce sandy/desert soil. It was found that inoculated showed high to water-deficient conditions delaying dehydration maintaining plant’s water at an optimal level. had recovery rate after rewatering interms similar biomass flowering compared non-stressed plants. These enhance also elicit induced systemic resistance scarcity. root architecture, thereby improving nutrient uptake. promoted stress-responsive metabolites sugars, sugar alcohols. play substantial role development strengthen defensive system various biotic abiotic stresses, particular stress.

Language: Английский

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The Stress-Responsive Alternative Sigma Factor SigB of Bacillus subtilis and Its Relatives: An Old Friend With New Functions

Frontiers in Microbiology, Journal Year: 2020, Volume and Issue: 11

Published: Sept. 15, 2020

Alternative sigma factors led the core RNA polymerase (RNAP) to recognize different sets of promoters those recognized by housekeeping A-directed RNAP. This change in RNAP promoter selectivity allows a rapid and flexible reformulation genetic program face environmental metabolic stimuli that could compromise bacterial fitness. The model bacterium Bacillus subtilis constitutes matchless living system study role alternative gene regulation physiology. SigB from B. sultilis was first factor described bacteria. Studies during last 40 years have shown it controls universe more than 150 genes playing crucial roles stress response, adaption survival. Activation relies on three separate pathways specifically respond energy, low temperature stresses. homologs, present other Gram-positive bacteria, also play important virulence against mammals. Interestingly, recent years, others unexpected responses, were found be controlled SigB. In particular, efficiencies spore biofilm formation, two features critical for adaptation survival planktonic sessile communities. subtilis, induces expression Spo0E aspartyl-phosphatase responsible blockage sporulation initiation. upregulated activity interconnects predominant adaptive (i.e., response) subtilis. addition, RsbP serine-phosphatase, belonging energy arm regulatory cascade, key transcription SinR decide whether cells residing remain mantain growth or scape colonize new niches through dispersal. Finally, intervenes recognition response surrounding microorganisms, role, agronomic impact. is induced when confronted with phytopathogenic fungi (e.g., Fusarium verticillioides) halts fungal benefit plant growth. this review, we update knowledge networks controlling activation its years.

Language: Английский

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Pulcherrimin formation controls growth arrest of the Bacillus subtilis biofilm

Proceedings of the National Academy of Sciences, Journal Year: 2019, Volume and Issue: 116(27), P. 13553 - 13562

Published: June 19, 2019

Biofilm formation by Bacillus subtilis is a communal process that culminates in the of architecturally complex multicellular communities. Here we reveal transition biofilm into nonexpanding phase constitutes distinct step development. Using genetic analysis show B. strains lacking ability to synthesize pulcherriminic acid form biofilms sustain expansion phase, thereby linking growth arrest. However, production not sufficient block biofilm. It needs be secreted extracellular environment where it chelates Fe 3+ from medium nonenzymatic reaction. Utilizing mathematical modeling and series experimental methodologies when level freely available iron drops below critical threshold, stops. Bioinformatics allows us identify genes required for synthesis other Firmicutes but patchwork presence both within across closely related species suggests loss these through multiple independent recombination events. The seemingly counterintuitive self-restriction led explore if there were any benefits associated with production. We identified producers can prevent invasion neighboring communities generation an “iron-free” zone, addressing paradox .

Language: Английский

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Method for rapid biofilm cultivation on microplastics and investigation of its effect on the agglomeration and removal of microplastics using organosilanes

The Science of The Total Environment, Journal Year: 2021, Volume and Issue: 806, P. 151388 - 151388

Published: Nov. 3, 2021

Language: Английский

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Complex extracellular biology drives surface competition during colony expansion in Bacillus subtilis

The ISME Journal, Journal Year: 2022, Volume and Issue: 16(10), P. 2320 - 2328

Published: July 5, 2022

Language: Английский

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Fungal hyphae colonization by Bacillus subtilis relies on biofilm matrix components

Biofilm, Journal Year: 2019, Volume and Issue: 1, P. 100007 - 100007

Published: Oct. 25, 2019

Bacteria interact with their environment including microbes and higher eukaryotes. The ability of bacteria fungi to affect each other are defined by various chemical, physical biological factors. During association, bacterial cells can directly attach settle on the hyphae fungal species. Such colonization mycelia was proposed be dependent biofilm formation bacteria, but essentiality matrix not represented before. Here, we demonstrate that secreted components soil-dwelling bacterium, Bacillus subtilis essential for establishment a dense population filamentous black mold fungus, Aspergillus niger basidiomycete mushroom, Agaricus bisporus. We further illustrate these shared among mutants highlighting community shaping impact formers bacteria-fungi interactions.

Language: Английский

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