LWT,
Journal Year:
2024,
Volume and Issue:
198, P. 115924 - 115924
Published: March 3, 2024
Aspergillus
flavus
and
the
produced
aflatoxins
are
common
hazardous
contaminants
in
grains
food,
which
generated
great
damage
to
food
security
human
health.
Acetyl-CoA
carboxylase
(ACC),
catalyzing
acetyl-CoA
malonyl-CoA,
is
first
speed-determining
step
fatty
acid
biosynthesis
(cell
membrane
composition)
process
A.
flavus.
Here,
we
demonstrated
that
dimethyl
trisulfide
(DMTS),
emitted
from
bacteria,
could
interact
with
ACC,
decrease
enzymatic
activity
377.62
191.42
kat/g
vitro,
down-regulate
expression
of
ACC
other
important
genes
aflatoxin
pathway,
reduce
content
hexadecanoic
acid,
octadecanoic
eicosanoic
finally
prevent
growth
production.
Two
binding
sites
containing
19
amino
acids
were
detected
between
DMTS
through
molecular
docking
analysis.
These
highly
conserved
different
filamentous
fungi,
accounted
for
broad
antifungal
spectrum
DMTS.
Therefore,
concluded
target
on
results
elucidate
inhibitory
mechanism
fungal
pathogens,
provide
basic
theories
development
novel
agents.
Food Bioscience,
Journal Year:
2023,
Volume and Issue:
52, P. 102422 - 102422
Published: Jan. 27, 2023
In
this
study,
16
Lactobacilli
(LABs)
isolated
from
the
drupes
of
olive
(Olea
europaea)
oil
varieties
were
identified
as
Lactiplantibacillus
plantarum
(seven
isolates),
Pediococcus
pentosaceus
(six
Enterococcus
faecium
(two
isolates)
and
Streptococcus
salivarius
(a
single
isolate)
by
peptide
mass
fingerprinting
sequencing
16S
rRNA.
Antifungal
activity
LABs
their
cell-free
fermentates
(CFSs)
against
several
plant
pathogenic
fungi
oomycetes
(fungi
sensu
lato),
including
Alternaria,
Aspergillus
Colletotrichum,
Penicillium,
Plenodomus
Phytophthora,
was
evaluated
in
vitro
using
culture
overlaying
agar
diffusion
tests.
Minimal
inhibitory
concentration
(MIC)
minimal
fungicidal
(MFC)
determined.
showed
antifungal
lato
tested.
The
most
noticeable
shown
isolates
L.
P.
Fusarium
oxysporum,
Colletotrichum
species
Penicillium
nordicum.
Chemical
analysis
revealed
CFSs
contained
acid
lactic
variable
quantities
14
diverse
phenolic
acids
26
volatile
organic
compounds
(VOCs).
No
obvious
correlation
found
between
metabolic
profile
efficacy.
However,
it
is
first
time
that
potential
LABs,
recovered
varieties,
natural
fungicides,
demonstrated.
International Journal of Molecular Sciences,
Journal Year:
2025,
Volume and Issue:
26(3), P. 985 - 985
Published: Jan. 24, 2025
Fungal
infections
are
a
significant
global
public
health
challenge
because
of
their
widespread
occurrence,
morbidity,
and
profound
social
economic
consequences.
Antifungal
resistance
is
also
an
increasing
concern,
posing
substantial
risk
to
health.
There
growing
interest
in
searching
for
new
antifungal
drugs
isolated
from
natural
sources.
This
study
aimed
evaluate
the
activity
novel
mollusk
fractions
against
fungal
strains
resistant
nystatin
amphotericin
B.
In
addition,
role
oxidative
stress
mechanism
damage
was
determined.
The
mucus
garden
snail
Cornu
aspersum
(MCa/1-20)
hemolymph
fraction
marine
Rapana
venosa
(HLRv/3-100)
were
obtained
characterized
via
12%
sodium
dodecyl
sulfate–polyacrylamide
gel
electrophoresis
(SDS-PAGE)
mass
spectrometric
-analyses.
results
demonstrate
that
spores
biomass
both
have
fungicidal
effect
Penicillium
griseofulvum,
Aspergillus
niger.
Compared
control
group,
release
intracellular
proteins
reducing
sugars
significantly
increased
treated
groups.
data
showed
levels
biomarkers
(lipid
peroxidation
oxidatively
damaged
proteins)
downregulated
antioxidant
enzyme
defense,
corresponding
activity.
To
our
knowledge,
this
first
evaluating
as
factor
fractions’
Foods,
Journal Year:
2025,
Volume and Issue:
14(4), P. 682 - 682
Published: Feb. 17, 2025
This
investigation
evaluates
the
comparative
efficacy
of
Cinnamomum
burmannii
leaf
essential
oil
(YXYO)
and
its
main
active
ingredients
as
a
novel
preservative
to
protect
stored
food
commodities
from
fungal
infestations,
aflatoxin
B1
(AFB1)
contamination
caused
by
Aspergillus
flavus.
Morphological
observations
utilizing
SEM
TEM
revealed
significant
alterations
in
treated
samples,
alongside
decrease
ergosterol
content
dose-dependent
disruption
antioxidant
system
energy
system.
Transcriptomic
analysis
suggested
that
differentially
expressed
genes
were
predominantly
associated
with
spore
growth,
cell
wall,
membrane,
oxidative
stress,
metabolism,
biosynthesis.
Solid-phase
microextraction-gas
chromatography-mass
spectrometry
(SPME-GC-MS)
identified
ten
YXYO,
including
borneol,
α-terpineol,
terpinen-4-ol,
etc.
Moreover,
an
effective
inhibition
A.
flavus
infection
peanuts
was
observed
application
30
μL/disc
YXYO
blend
compounds.
