3D printing of hydrogels: Rational design strategies and emerging biomedical applications

Materials Science and Engineering R Reports, Journal Year: 2020, Volume and Issue: 140, P. 100543 - 100543

Published: Feb. 18, 2020

3D printing alias additive manufacturing can transform virtual models created by computer-aided design (CAD) into physical objects in a layer-by-layer manner dispensing with conventional molding or machining. Since the incipiency, significant advancements have been achieved understanding process of and relationship component, structure, property application objects. Because hydrogels are one most feasible classes ink materials for this field has rapidly advancing, Review focuses on hydrogel designs development advanced hydrogel-based biomaterial inks bioinks printing. It covers techniques including laser (stereolithography, two-photon polymerization), extrusion (3D plotting, direct writing), inkjet printing, bioprinting, 4D bioprinting. provides comprehensive overview discussion tailorability material, mechanical, physical, chemical biological properties to enable The range hydrogel-forming polymers covered encompasses biopolymers, synthetic polymers, polymer blends, nanocomposites, functional cell-laden systems. representative biomedical applications selected demonstrate how is being exploited tissue engineering, regenerative medicine, cancer research, vitro disease modeling, high-throughput drug screening, surgical preparation, soft robotics flexible wearable electronics. Incomparable thermoplastics, thermosets, ceramics metals, playing pivotal role creation (bio)systems customizable way. An outlook future directions presented.

Language: Английский

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Hydrogel Bioink Reinforcement for Additive Manufacturing: A Focused Review of Emerging Strategies

Advanced Materials, Journal Year: 2019, Volume and Issue: 32(1)

Published: Oct. 10, 2019

Abstract Bioprinting is an emerging approach for fabricating cell‐laden 3D scaffolds via robotic deposition of cells and biomaterials into custom shapes patterns to replicate complex tissue architectures. uses hydrogel solutions called bioinks as both cell carriers structural components, requiring be highly printable while providing a robust cell‐friendly microenvironment. Unfortunately, conventional have not been able meet these requirements are mechanically weak due their heterogeneously crosslinked networks lack energy dissipation mechanisms. Advanced bioink designs using various methods dissipating mechanical aimed at developing next‐generation cellularized mimic anatomical size, architecture, tissue‐specific functions. These need high print fidelity should provide biocompatible microenvironment along with improved properties. To design advanced formulations, it important understand the structure–property–function relationships networks. By specifically leveraging biophysical biochemical characteristics networks, performance can designed control direct In this review article, current approaches in reinforcement techniques critically evaluated. This bottom‐up perspective provides materials‐centric bioprinting.

Language: Английский

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Three-dimensional bioprinting of gelatin methacryloyl (GelMA)

Bio-Design and Manufacturing, Journal Year: 2018, Volume and Issue: 1(4), P. 215 - 224

Published: Nov. 16, 2018

Language: Английский

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Natural Biomaterials and Their Use as Bioinks for Printing Tissues

Bioengineering, Journal Year: 2021, Volume and Issue: 8(2), P. 27 - 27

Published: Feb. 20, 2021

The most prevalent form of bioprinting—extrusion bioprinting—can generate structures from a diverse range materials and viscosities. It can create personalized tissues that aid in drug testing cancer research when used combination with natural bioinks. This paper reviews bioinks their properties functions hard soft tissue engineering applications. discusses agarose, alginate, cellulose, chitosan, collagen, decellularized extracellular matrix, dextran, fibrin, gelatin, gellan gum, hyaluronic acid, Matrigel, silk. Multi-component are considered as way to address the shortfalls individual biomaterials. mechanical, rheological, cross-linking along cytocompatibility, cell viability, printability detailed well. Future avenues for into then presented.

Language: Английский

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Extrusion bioprinting: Recent progress, challenges, and future opportunities

Bioprinting, Journal Year: 2020, Volume and Issue: 21, P. e00116 - e00116

Published: Nov. 23, 2020

Language: Английский

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Hydrogels for Bioprinting: A Systematic Review of Hydrogels Synthesis, Bioprinting Parameters, and Bioprinted Structures Behavior

Frontiers in Bioengineering and Biotechnology, Journal Year: 2020, Volume and Issue: 8

Published: Aug. 6, 2020

Nowadays, bioprinting is rapidly evolving and hydrogels are a key component for its success. In this sense, synthesis of hydrogels, as well process, cross-linking bioinks represent different challenges the scientific community. A set unified criteria common framework missing, so multidisciplinary research teams might not efficiently share advances limitations bioprinting. Although multiple combinations materials proportions have been used several applications, it still unclear relationship between good printability better medical/clinical behavior bioprinted structures. For reason, PRISMA methodology was conducted in review. Thus, 1,774 papers were retrieved from PUBMED, WOS, SCOPUS databases. After selection, 118 analyzed to extract information about materials, hydrogel synthesis, tests performed on The aim systematic review analyze their influence parameters that ultimately generate tridimensional Furthermore, comparison mechanical cellular those structures presented. Finally, some conclusions recommendations exposed improve reproducibility facilitate fair results.

Language: Английский

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On the progress of hydrogel-based 3D printing: Correlating rheological properties with printing behaviour

International Journal of Pharmaceutics, Journal Year: 2022, Volume and Issue: 615, P. 121506 - 121506

Published: Jan. 24, 2022

One of the exciting future directions in 3D printing field is development innovative personalized smart constructions for bio-applications, including drug delivery, namely high-throughput screening and customized topical/oral administration pharmaceuticals, as well tissue engineering. In this context, hydrogels have emerged a promising material that, when combined with extrusion printing, allow creation soft-material structures defined spatial locations, that can be printed at room temperature by tuning geometric design and/or formulation components. Thus, efficacy quality such vehicles dependent on formulation, design, process parameters. However, hydrogel inks are often designed characterized using different methods lack uniformity impairs. Characterization techniques usually arbitrary differ among research groups, challenging inference possible conclusions behaviour potential applications. Therefore, to properly analyse particular ink we review, first time, most frequently employed characterization procedures, from rheological approaches parameters settings, discuss their relevance, limitations drawbacks, highlight perspectives. Overall, accelerate high-quality constructs, comprehensive protocols both pre-printing assays should adopted. Furthermore, transversal adoption could serve boost terms requirements regulatory aspects.

Language: Английский

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3D Bioprinted Scaffolds for Bone Tissue Engineering: State-Of-The-Art and Emerging Technologies

Frontiers in Bioengineering and Biotechnology, Journal Year: 2022, Volume and Issue: 10

Published: April 11, 2022

Treating large bone defects, known as critical-sized defects (CSDs), is challenging because they are not spontaneously healed by the patient's body. Due to limitations associated with conventional grafts, tissue engineering (BTE), based on three-dimensional (3D) bioprinted scaffolds, has emerged a promising approach for reconstitution and treatment. Bioprinting technology allows incorporation of living cells and/or growth factors into scaffolds aiming mimic structure properties native bone. To date, wide range biomaterials (either natural or synthetic polymers), well various factors, have been explored use in scaffold bioprinting. However, key challenge that remains fabrication meet structure, mechanical, osteoconductive requirements support vascularization. In this review, we briefly present latest developments discoveries CSD treatment means focus biomaterials, cells, formulating bioinks their bioprinting techniques. Promising state-of-the-art pathways strategies recently developed highlighted, including bioactive ceramics create composite advanced technologies (e.g., core/shell bioprinting) form hybrid systems, rigorous design taking account influence such parameters pore geometry porosity. We also review in-vitro assays in-vivo models track regeneration, followed discussion current 3D BTE. conclude emerging approaches field, development gradient four-dimensional (4D) printing via smart materials, organoids, cell aggregates/spheroids along future avenues related

Language: Английский

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3D bioprinted organ‐on‐chips

Aggregate, Journal Year: 2022, Volume and Issue: 4(1)

Published: May 1, 2022

Abstract Organ‐on‐a‐chip (OOC) platforms recapitulate human in vivo‐like conditions more realistically compared to many animal models and conventional two‐dimensional cell cultures. OOC setups benefit from continuous perfusion of cultures through microfluidic channels, which promotes viability activities. Moreover, chips allow the integration biosensors for real‐time monitoring analysis interactions responses administered drugs. Three‐dimensional (3D) bioprinting enables fabrication multicell with sophisticated 3D structures that closely mimic tissues. 3D‐bioprinted are promising tools understanding functions organs, disruptive influences diseases on organ functionality, screening efficacy as well toxicity drugs organs. Here, common techniques, advantages, limitations each method reviewed. Additionally, recent advances, applications, potentials emulating various organs presented. Last, current challenges future perspectives discussed.

Language: Английский

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A Guide to Polysaccharide-Based Hydrogel Bioinks for 3D Bioprinting Applications

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(12), P. 6564 - 6564

Published: June 12, 2022

Three-dimensional (3D) bioprinting is an innovative technology in the biomedical field, allowing fabrication of living constructs through approach layer-by-layer deposition cell-laden inks, so-called bioinks. An ideal bioink should possess proper mechanical, rheological, chemical, and biological characteristics to ensure high cell viability production tissue with dimensional stability shape fidelity. Among several types bioinks, hydrogels are extremely appealing as they have many similarities extracellular matrix, providing a highly hydrated environment for proliferation tunability terms mechanical rheological properties. Hydrogels derived from natural polymers, polysaccharides, particular, excellent platform mimic given their low cytotoxicity, hydrophilicity, diversity structures. In fact, polysaccharide-based trendy materials 3D since abundant combine adequate physicochemical biomimetic features development novel Thus, this review portrays most relevant advances hydrogel bioinks bioprinting, focusing on last five years, emphasis properties, advantages, limitations, considering polysaccharide families classified according source, namely seaweed, higher plants, microbial, animal (particularly crustaceans) origin.

Language: Английский

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