The Ethanol-Producing Enterocloster bolteae and Limosilactobacillus fermentum are enriched in Nonalcoholic steatohepatitis DOI Creative Commons

Babacar Mbaye,

Reham Magdy Wasfy,

Patrick BORENTAIN

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: July 26, 2023

Abstract Background: Non-alcoholic steatohepatitis (NASH) has become a major public health issue. In fact, it is one of the leading causes disturbed liver function and transplantation worldwide. Its link with intestinal microbiota still topical, production endogenous ethanol following dysbiosis - mechanisms linked to disease been shown in several studies. this study, we analyzed stool samples from 41 NASH patients 24 healthy controls using culturomics 16S rRNA targeted metagenomics identify microbial profile associated each group. Fecal concentration was also determined for all samples. Results: We identified 358 different bacterial species approach distributed into 11 phyla 143 genera. With significant difference, 12 appeared significantly more frequently group 4 control Finegoldia magna most detected (10/14 (71%)), 2 producing strains: Limosilactobacillus fermentum Enterocloster bolteae. Metagenomics shows an enrichment Lactobacillus Streptococcus identical that culture. regard species, there elevated frequency ethanol-producing bacteria patients, higher stools compared (bilateral Mann-Whitney test, p=value 0.0145). However, metagenomics, only culture showed bolteae patients. Conclusions: Culturomics are complementary methods exploring microbiota. Our results suggest may play instrumental role pathogenesis disease.

Language: Английский

Updated mechanisms of MASLD pathogenesis DOI Creative Commons
Yuxuan Li,

Peipei Yang,

Jialu Ye

et al.

Lipids in Health and Disease, Journal Year: 2024, Volume and Issue: 23(1)

Published: April 22, 2024

Abstract Metabolic dysfunction-associated steatotic liver disease (MASLD) has garnered considerable attention globally. Changing lifestyles, over-nutrition, and physical inactivity have promoted its development. MASLD is typically accompanied by obesity strongly linked to metabolic syndromes. Given that prevalence on the rise, there an urgent need elucidate pathogenesis. Hepatic lipid accumulation generally triggers lipotoxicity induces or progress steatohepatitis (MASH) mediating endoplasmic reticulum stress, oxidative organelle dysfunction, ferroptosis. Recently, significant been directed towards exploring role of gut microbial dysbiosis in development MASLD, offering a novel therapeutic target for MASLD. Considering are no recognized pharmacological therapies due diversity mechanisms involved difficulty associated with undertaking clinical trials, potential targets remain elusive. Thus, this article aimed summarize evaluate prominent roles lipotoxicity, ferroptosis, microbes underlying their effects. Furthermore, existing advances challenges treatment were outlined.

Language: Английский

Citations

45
Effects of duo-strain probiotics on growth, digestion, and gut health in broiler chickens DOI Creative Commons
Seyed Mehrdad Mirsalami, Mahsa Mirsalami

Veterinary and Animal Science, Journal Year: 2024, Volume and Issue: 24, P. 100343 - 100343

Published: March 2, 2024

The goal of this inquiry was to analyze the impact incorporating

Language: Английский

Citations

21
Gut microbes in metabolic disturbances. Promising role for therapeutic manipulations? DOI
Piero Portincasa, Mohamad Khalil,

Annarita Graziani

et al.

European Journal of Internal Medicine, Journal Year: 2023, Volume and Issue: 119, P. 13 - 30

Published: Oct. 4, 2023

Language: Английский

Citations

29
Exploring the human archaeome: its relevance for health and disease, and its complex interplay with the human immune system DOI Creative Commons
Torben Kuehnast, Christina Kumpitsch,

Rokhsareh Mohammadzadeh

et al.

FEBS Journal, Journal Year: 2024, Volume and Issue: unknown

Published: March 31, 2024

This Review aims to coalesce existing knowledge on the human archaeome, a less‐studied yet critical non‐bacterial component of microbiome, with focus its interaction immune system. Despite largely bacteria‐centric in microbiome research, archaea present unique challenges and opportunities for understanding health. We examine archaeal distribution across different body sites, such as lower gastrointestinal tract (LGT), upper aerodigestive (UAT), urogenital (UGT), skin. Variability composition exists between sites; methanogens dominate LGT, while Nitrososphaeria are prevalent skin UAT. Archaea have be classified pathogens but show associations conditions refractory sinusitis vaginosis. In methanogenic play metabolic roles by converting bacterial end‐products into methane, correlating various health conditions, including obesity certain cancers. Finally, this work looks at complex interactions system molecular level. Recent research has illuminated specific molecules, RNA glycerolipids, stimulating responses via innate receptors like Toll‐like receptor 8 (TLR8) ‘C‐type lectin domain family 4 member E’ (CLEC4E; also known MINCLE). Additionally, by‐products archaea, specifically demonstrated immunomodulatory effects through anti‐inflammatory anti‐oxidative pathways. these advancements, mechanistic underpinnings how influence activity remain fertile area further investigation.

Language: Английский

Citations

11
Increased fecal ethanol and enriched ethanol-producing gut bacteria Limosilactobacillus fermentum, Enterocloster bolteae, Mediterraneibacter gnavus and Streptococcus mutans in nonalcoholic steatohepatitis DOI Creative Commons

Babacar Mbaye,

Reham Magdy Wasfy,

Patrick Borentain

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2023, Volume and Issue: 13

Published: Nov. 16, 2023

Background Non-alcoholic steatohepatitis (NASH) has become a major public health issue as one of the leading causes liver disease and transplantation worldwide. The instrumental role gut microbiota is emerging but still under investigation. Endogenous ethanol (EtOH) production by bacteria yeasts an putative mechanism. Microbial metagenomics culture studies targeting enterobacteria or have been reported, no culturomics conducted so far. Aim To assess fecal EtOH other biochemical parameters, characterize NASH-associated dysbiosis identify EtOH-producing microbes associated with disease, samples from 41 NASH patients 24 controls were analyzed. High-performance liquid chromatography (HPLC) was used for EtOH, glucose, total proteins, triglyceride cholesterol. Viable assessed microbial culturomics. genetic material using 16S hypervariable V3V4 region. Results Fecal glucose elevated in stools (p < 0.05) not triglyceride, cholesterol proteins. In culturomics, Enterocloster bolteae Limosilactobacillus fermentum enriched NASH. rRNA amplicon sequencing confirmed enrichment including L. , Mediterraneibacter gnavus Streptococcus mutans species previously dysbiosis-associated diseases. Strikingly, E. identified only well-known Lacticaseibacillus casei never isolated 0.05). Conclusion Elevated feature Several different may play disease. Culturomics metagenomics, two complementary methods, will be critical to future diagnostic markers therapeutic targets Suppression administration are options tested treatment.

Language: Английский

Citations

15
Candida tropicalis, Clavispora lusitaniae, Limosilactobacillus fermentum, Liquorilactobacillus mali, and Leuconostoc pseudomesenteroides are associated with Ethanol Levels in Malian Traditional Fermented Milk Products DOI Creative Commons

Sibiri Sissoko,

Salimata Konaté,

Nicholas Armstrong

et al.

Microbial Pathogenesis, Journal Year: 2025, Volume and Issue: unknown, P. 107298 - 107298

Published: Jan. 1, 2025

Language: Английский

Citations

0
A study of correlation of the dietary index for gut microbiota with non-alcoholic fatty liver disease based on 2007–2018 National Health and Nutrition Examination Survey DOI Creative Commons
Yinda Wang, Binzhong Zhang,

Lianzhong Feng

et al.

Frontiers in Nutrition, Journal Year: 2025, Volume and Issue: 12

Published: April 10, 2025

To explore the correlation of dietary index for gut microbiota (DI-GM) with non-alcoholic fatty liver disease (NAFLD). Data 6,711 participants were extracted from National Health and Nutrition Examination Survey (NHANES) during 2007-2018. A weighted logistic regression analysis was employed assessment DI-GM NAFLD, a restricted cubic spline (RCS) implemented to examine potential non-linear associations. Subgroup analyses conducted identify particularly susceptible groups. Additionally, synergistic effects different components on NAFLD risk assessed by quantile sum (WQS) regression. The exhibited statistically significant [OR (95%CI):0.91 (0.85, 0.98), p = 0.015]. results RCS indicated linear (p 0.810 non-linearity). Further stratified that negative consistent all subgroups. WQS revealed soybean (27%), refined grains (17%), coffee (16%), red meat (9%) had highest contribution weights NAFLD. As an important tool influences diet microbiota, is negatively correlated factors. Soybean, grains, coffee, are key factors influencing direct shall be explored effectiveness prevention treatment evaluated improving scores via interventions.

Language: Английский

Citations

0
Understanding the role of Hedgehog signaling pathway and gut dysbiosis in fueling liver cancer DOI
Naz Fatima,

Hooriya Fatima,

Sadia Ahmad

et al.

Molecular Biology Reports, Journal Year: 2025, Volume and Issue: 52(1)

Published: April 22, 2025

Language: Английский

Citations

0
The Microbiome and Metabolic Dysfunction-Associated Steatotic Liver Disease DOI Open Access
Diren Beyoğlu, Jeffrey R. Idle

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(7), P. 2882 - 2882

Published: March 22, 2025

Metabolic dysfunction-associated steatotic liver disease (MASLD) is a condition wherein excessive fat accumulates in the liver, leading to inflammation and potential damage. In this narrative review, we evaluate tissue microbiota, how they arise their constituent microbes, role of intestinal hepatic microbiota MASLD. The history bacteriophages (phages) occurrence part causation MASLD, conversely, "phage therapy" for antibiotic resistance, obesity, are all described. metabolism bile acids dietary tryptophan histidine defined, together with impacts individual metabolites on MASLD pathogenesis. Both periodontitis dysbiosis may cause microorganisms involved these processes discussed. Novel treatment opportunities involving exist include fecal transplantation, probiotics, prebiotics, synbiotics, supplements, intermittent fasting, phages or holins endolysins. Although FDA yet approve phage therapy clinical use, there multiple FDA-approved trials, represent new horizon future

Language: Английский

Citations

0
A catalog of ethanol-producing microbes in humans DOI

Babacar Mbaye,

Reham Magdy Wasfy, Maryam Tidjani Alou

et al.

Future Microbiology, Journal Year: 2024, Volume and Issue: 19(8), P. 697 - 714

Published: May 3, 2024

Aim: Endogenous ethanol production emerges as a mechanism of nonalcoholic steatohepatitis, obesity, diabetes and auto-brewery syndrome. Methods: To identify ethanol-producing microbes in humans, we used the NCBI taxonomy browser PubMed database with an automatic query manual verification. Results: 85 human were identified. Saccharomyces cerevisiae, Candida Pichia most represented fungi. Enterobacteriaceae was bacterial family mainly Escherichia coli Klebsiella pneumoniae. Species Lachnospiraceae Clostridiaceae family, Lactobacillales order Bifidobacterium genus also Conclusion: This catalog will help study pathophysiology, diagnosis, prevention management diseases associated endogenous production.

Language: Английский

Citations

3