Estudio del rol del segundo mensajero c-di-GMP en la regulación de fenotipos asociados a la patogénesis de <i>Bordetella pertussis</i> DOI Creative Commons

Federico Zacca

Published: April 14, 2023

El género Bordetella está compuesto por especies que pueden causar infecciones respiratorias en diversos organismos. Entre ellas se encuentra pertussis, un patógeno exclusivo de humanos y agente causal la enfermedad conocida como tos convulsa. Para llevar a cabo infección, B. pertussis expresa una serie factores virulencia encuentran regulados principalmente actividad del sistema dos componentes BvgAS. Sin embargo, los últimos años han surgido evidencias demuestran hay otros mecanismos regulatorios adicionales involucrados expresión virulencia. En nuestro laboratorio, nos encontramos abocados al estudio señalización mediado el segundo mensajero c-di-GMP su rol patogénesis bacterias Bordetella. Los niveles célula controlados familias proteínas: diguanilato ciclasas fosfodiesterasas, sintetizan degradan mensajero, respectivamente. Estas proteínas identificarse genomas bacterianos debido presencia dominios conservados: GG(D/E)EF para las EAL o HD-GYP fosfodiesterasas. este contexto, objetivo presente trabajo tesis es evaluar si importante control procesos asociados pertussis. ello, centramos particular: BP1492, proteína con dominio GG(D/E)EF, BP1092, “dual” posee GGDEF, histidina quinasa. A través utilización diversas técnicas microbiología biología molecular pudimos evidenciar BP1492 ciclasa activa, capaz modificar fenotipos estaría involucrada resistencia pH ácido Por otra parte, describimos mecanismo local entre BP1092 ciclasa, podría regular asociadas conclusión, obtuvieron resultados regula podrían estar relacionados realiza aporte comprensión moleculares regulatorio.

Cross-Talk Between Signal Transduction Systems and Metabolic Networks in Bacterial Antibiotics Resistance and Tolerance DOI
Shuji Gao, Baobao Liu, Shuo Yuan

et al.

International Journal of Antimicrobial Agents, Journal Year: 2025, Volume and Issue: unknown, P. 107479 - 107479

Published: Feb. 1, 2025

Language: Английский

Citations

0
Antimicrobial peptides: from discovery to developmental applications DOI Creative Commons
Qi Zhang

Applied and Environmental Microbiology, Journal Year: 2025, Volume and Issue: unknown

Published: April 3, 2025

Antimicrobial resistance (AMR) has emerged as a significant crisis in global health. Due to their advantageous properties, antimicrobial peptides (AMPs) have garnered considerable attention potential alternative therapy address the AMR crisis. These might disrupt cell membranes or walls exhibit activity, modulate immune response promote recovery from diseases. In recent years, progress been made research of AMPs, alongside emergence new challenges. This review first systematically summarizes and critically discusses advancements understanding characteristics current landscapes well regulatory mechanisms action practical applications, particularly those reported approved within last 5 years. Additionally, principles, paths for identification, future trends AMPs are also analyzed following discussion advantages disadvantages comparison conventional antibiotics. Unlike prior literature this field, report summarized latest major discovery methods and, more importantly, emphasized applications by supporting various viewpoints using selected examples AMPs' real-life scenarios. Besides, some emerging hot topics including derived gut microbiota synergistic effects combating AMR, were profiled. All these indicate originality provide valuable references AMP discoveries applications.

Language: Английский

Citations

0
Mechanism of extracellular polymeric substance production and its contribution to anaerobic digestion foaming: Insights from cellular signalling DOI
Wenjie Ye, Lei Li,

Pingjin Yang

et al.

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: unknown, P. 157377 - 157377

Published: Nov. 1, 2024

Language: Английский

Citations

3
c-di-GMP and AHL signals-triggered chemical communication under electrical signaling disruption restores Geobacter sulfurreducens biofilm formation DOI Creative Commons
Qian Zhu, Yanyan Zheng, Xingwang Zhou

et al.

ISME Communications, Journal Year: 2024, Volume and Issue: 4(1)

Published: Jan. 1, 2024

Abstract Electrogenic biofilms, which have attracted considerable attention in simultaneous wastewater treatment and energy recovery bioelectrochemical systems, are regulated by chemical communication potassium channel-mediated electrical signaling. However, how these two pathways interact with each other has not been thoroughly investigated. This study first explored the roles of communication, including intracellular bis-(3′-5′)-cyclic dimeric guanosine monophosphate (c-di-GMP) extracellular N-acyl-homoserine lactone (AHL)-mediated quorum sensing, electrogenic biofilm formation through an integrated analysis transcriptomics metabolomics. Electrical signaling disruption inhibited electroactivity Geobacter sulfurreducens biofilm, was mainly ascribed to reduction viability protein/polysaccharide ratio. The upregulation expression levels genes encoding c-di-GMP AHL synthesis transcriptomic analysis, increased secretion N-butanoyl-L-homoserine metabolomic confirmed enhancement under disruption, thus indicating a compensatory mechanism among different pathways. Furthermore, protein–protein interaction network showed convergence pathways, c-di-GMP-related acting as central bridges. highlights especially resilience adverse external stresses, thereby laying foundation for facilitating conditions practical applications.

Language: Английский

Citations

2
Oxidative stress response in avian pathogenic Escherichia coli DOI
Lumin Yu, Hui Wang,

Xinglin Zhang

et al.

Research in Veterinary Science, Journal Year: 2024, Volume and Issue: 180, P. 105426 - 105426

Published: Sept. 25, 2024

Language: Английский

Citations

2
Decoding Bacterial Communication: Intracellular Signal Transduction, Quorum Sensing, and Cross-Kingdom Interactions DOI
Shuxun Liu,

Xujie Feng,

Hangjia Zhang

et al.

Microbiological Research, Journal Year: 2024, Volume and Issue: 292, P. 127995 - 127995

Published: Dec. 7, 2024

Language: Английский

Citations

2
Structures of the DarR transcription regulator reveal unique modes of second messenger and DNA binding DOI Creative Commons
Maria A. Schumacher,

Nicholas Lent,

Vincent B. Chen

et al.

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: Nov. 9, 2023

Abstract The mycobacterial repressor, DarR, a TetR family regulator (TFR), was the first transcription shown to bind c-di-AMP. However, molecular basis for this interaction and mechanism involved in DNA binding by DarR remain unknown. Here we describe DarR-c-di-AMP DarR-DNA structures complementary biochemical assays. structure reveals unique effector site TFR, located between dimer subunits. Strikingly, show motif also binds cAMP. location of adenine nucleotide subunits suggests may facilitate dimerization hence binding. Indeed, assays cAMP enhances Finally, reveal distinct TFR DNA-binding involving two interacting dimers on DNA. Thus, combined data unveil newly described second messenger mode important regulators.

Language: Английский

Citations

4
Old role with new feature: T2SS ATPase as a cyclic-di-GMP receptor to regulate antibiotic production DOI

Haofei Liu,

Gaoge Xu, Baodian Guo

et al.

Applied and Environmental Microbiology, Journal Year: 2024, Volume and Issue: 90(5)

Published: April 16, 2024

ABSTRACT Cyclic di-GMP (c-di-GMP) is a crucial signaling molecule found extensively in bacteria, involved the regulation of various physiological and biochemical processes such as biofilm formation, motility, pathogenicity through binding to downstream receptors. However, structural dissimilarity c-di-GMP receptor proteins has hindered discovery many proteins. In this study, we identified LspE, homologous protein type II secretion system (T2SS) ATPase GspE Lysobacter enzymogenes , for c-di-GMP. We more conservative amino acid residues K358 T359, which differ from previous reports, indicating that may represent class Additionally, LspE L. also possesses novel role regulating production antifungal antibiotic HSAF. Further investigations revealed critical involvement both activity LspE-mediated HSAF (Heat-Stable Antifungal Factor) production, with having no impact on LspE’s activity. This suggests control by encompasses two distinct processes: inherent LspE. Overall, our study unraveled new function conventional T2SS shed light its production. IMPORTANCE The pathway bacteria highly intricate. identification functional characterization have posed significant challenge research. well-studied bacteria. findings protein. Notably, discovered . Given be conserved protein, it worthwhile researchers reevaluate roles mechanisms across diverse bacterial species.

Language: Английский

Citations

1
Adaptive evolutionary trajectories in complexity: repeated transitions between unicellularity and differentiated multicellularity DOI Creative Commons
Hanna Isaksson, Peter A. Lind, Eric Libby

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: May 17, 2024

Multicellularity spans a wide gamut in terms of complexity, from simple clonal clusters cells to large-scale organisms composed differentiated and tissues. While recent experiments have demonstrated that forms multicellularity can readily evolve response different selective pressures, it is unknown if continued exposure those same pressures will result the evolution increased multicellular complexity. We use mathematical models consider adaptive trajectories unicellular exposed periodic bouts abiotic stress, such as drought or antibiotics. Populations improve survival stress by evolving cell differentiation—or both; however, these responses associated costs when absent. define parameter space fitness-relevant traits identify where multicellularity, differentiation, their combination fittest. then study effects adaptation allowing populations fix mutations fitness. find while mutation be beneficial phenotypes with e.g. unicellularity magnitudes differ alter which phenotype As result, we observe gain lose also show order mutations, historical contingency, cause some transitions permanent absence neutral evolution. Ultimately, driver for either lead increasing complexity return unicellularity.

Language: Английский

Citations

1
Adaptive laboratory evolution of Rhodococcus rhodochrous DSM6263 for chlorophenol degradation under hypersaline condition DOI Creative Commons
Jie Zheng, Zheng‐Zhi Zhang,

Juan An

et al.

Microbial Cell Factories, Journal Year: 2023, Volume and Issue: 22(1)

Published: Oct. 26, 2023

Normally, a salt amount greater than 3.5% (w/v) is defined as hypersaline. Large amounts of hypersaline wastewater containing organic pollutants need to be treated before it can discharged into the environment. The most critical aspect biological treatment saline inhibitory/toxic effect exerted on bacterial metabolism by high concentrations. Although efforts have been dedicated improving performance through use salt-tolerant or halophilic bacteria, diversities strains and range substrate spectrum remain limited, especially in chlorophenol treatment.In this study, chlorophenol-degrading strain was generated from Rhodococcus rhodochrous DSM6263, an original aniline degrader, adaptive laboratory evolution. evolved R. CP-8 could tolerant 8% NaCl with 4-chlorophenol degradation capacity. synonymous mutation phosphodiesterase may retard hydrolysis cyclic adenosine monophosphate (cAMP), which key factor reported osmoregulation. experimentally verified up-regulation intracellular cAMP level contributes improvement growth phenotype under osmotic condition. Additionally, point mutant catechol 1,2-dioxygenase, CatAN211S, revealed show 1.9-fold increment activity, mechanism well explained molecular docking analysis.This study developed one extraordinary capacity tolerance, showed great application potential treatment. resulted change concentration then increase tolerance strain. 1,2-dioxygenase improved activity also facilitated removal since enzyme pathway.

Language: Английский

Citations

2