General and specialized tyrosine metabolism pathways in plants

aBIOTECH, Journal Year: 2019, Volume and Issue: 1(2), P. 97 - 105

Published: Sept. 6, 2019

Language: Английский

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Metabolic Profiling of Pitaya (Hylocereus polyrhizus) during Fruit Development and Maturation

Molecules, Journal Year: 2019, Volume and Issue: 24(6), P. 1114 - 1114

Published: March 20, 2019

Pitaya (Hylocereus polyrhizus) has attracted much interest from consumers as it is a novelty fruit with high nutrient content and tolerance to drought stress. As group of attractive pigment- health-promoting natural compounds, betalains represent visual feature for pitaya quality. However, little information on the correlation between relevant metabolites exists so far. Currently, color (Commission International del'Eclairage, CIE) parameters, betalain contents, untargeted metabolic profiling (gas chromatography-time-of-flight-mass spectrometry, GC⁻MS liquid chromatography tandem mass LC⁻MS) have been examined 'Zihonglong' fruits at nine different developmental stages, variation character metabolite contents was simultaneously investigated peel pulp. Furthermore, principal component analysis (PCA) partial least-squares discriminant (PLS-DA) were used explore profiles samples. Our results demonstrated that decrease amino acid, accompanied by increase sugars organic might contribute formation betalains. Notably, one four potential biomarker metabolites, citramalic acid be related formation.

Language: Английский

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Development of active and smart packaging films based on starch, polyvinyl alcohol and betacyanins from different plant sources

International Journal of Biological Macromolecules, Journal Year: 2021, Volume and Issue: 183, P. 358 - 368

Published: April 28, 2021

Language: Английский

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Betanin as a multipath oxidative stress and inflammation modulator: a beetroot pigment with protective effects on cardiovascular disease pathogenesis

Critical Reviews in Food Science and Nutrition, Journal Year: 2020, Volume and Issue: 62(2), P. 539 - 554

Published: Sept. 30, 2020

Oxidative stress is a common physiopathological condition enrolled in risk factors for cardiovascular diseases. Individuals such redox imbalance status present endothelial dysfunctions and inflammation, reaching the onset of heart disease. Phytochemicals are able to attenuate main mechanisms oxidative inflammation should be considered as supportive therapies manage Beetroot (Beta vulgaris L.) rich source bioactive compounds, including betanin (betanidin-5-O-β-glucoside), pigment displaying potential alleviate inflammantion, previously demonstrated preclinical trials. Betanin resists gastrointestinal digestion, absorbed by epithelial cells intestinal mucosa reaches plasma its active form. displays free-radical scavenger ability through hydrogen or electron donation, preserving lipid structures LDL particles while inducing transcription antioxidant genes nuclear factor erythroid-2-related 2 and, simultaneously, suppressing pro-inflammatory kappa-B pathways. This review discusses anti-radical gene regulatory cardioprotective activities pathophysiology damage atherogenesis, conditions In addition, influences on these multipath cellular signals aiding reducing disorders proposed.

Language: Английский

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GATA and Phytochrome Interacting Factor Transcription Factors Regulate Light-Induced Vindoline Biosynthesis in Catharanthus roseus

PLANT PHYSIOLOGY, Journal Year: 2019, Volume and Issue: 180(3), P. 1336 - 1350

Published: May 13, 2019

Catharanthus roseus is the exclusive source of an array terpenoid indole alkaloids including anticancer drugs vincristine and vinblastine, derived from coupling catharanthine vindoline. Leaf-synthesized vindoline regulated by light. A seven-step enzymatic process involved in sequential conversion tabersonine to vindoline; however, regulatory mechanism controlling expression genes encoding these enzymes has not been elucidated. Here, we identified CrGATA1, Leu-Leu-Met domain GATA transcription factor that regulates light-induced biosynthesis C. seedlings. Expression CrGATA1 pathway T16H2, T3O, T3R, D4H, DAT was significantly induced In addition, activated promoters five light-responsive plant cells. Two GATC motifs D4H promoter were critical for CrGATA1-mediated transactivation. Transient overexpression seedlings resulted up-regulation increased accumulation. Conversely, virus-induced gene silencing young leaves repressed key reduced Furthermore, showed a Phytochrome Interacting Factor, CrPIF1, repressor biosynthesis. or CrPIF1 altered dark. activity binding G/E-box/PBE elements. Our findings reveal module involving Factor -GATA governs light-mediated specialized metabolites.

Language: Английский

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Metabolic engineering and synthetic biology of plant natural products – A minireview

Current Plant Biology, Journal Year: 2020, Volume and Issue: 24, P. 100163 - 100163

Published: July 8, 2020

Plant natural products include a diverse array of compounds that play important roles in plant metabolism and physiology. After elucidation biosynthetic pathways regulatory factors, it has become possible to metabolically engineer new capabilities planta as well successfully whole into microbial systems. Microbial expression systems for producing valuable have evolved incorporate polyculture co-culture consortiums carrying out robust biosynthesis strategies. This review focuses on four classes secondary metabolites the recent advances generating useful platforms metabolic engineering. They are flavonoids, alkaloids, betalains, glucosinolates.

Language: Английский

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A chromosome-scale genome sequence of pitaya (Hylocereus undatus) provides novel insights into the genome evolution and regulation of betalain biosynthesis

Horticulture Research, Journal Year: 2021, Volume and Issue: 8(1)

Published: July 6, 2021

Abstract Pitaya ( Hylocereus ) is the most economically important fleshy-fruited tree of Cactaceae family that grown worldwide, and it has attracted significant attention because its betalain-abundant fruits. Nonetheless, lack a pitaya reference genome significantly hinders studies focused on evolution, as well potential for genetic improvement this crop. Herein, we employed various sequencing approaches, namely, PacBio-SMRT, Illumina HiSeq paired-end, 10× Genomics, Hi-C (high-throughput chromosome conformation capture) to provide chromosome-level genomic assembly ‘GHB’ H. undatus , 2n = 2x 22 chromosomes). The size assembled was 1.41 Gb, with scaffold N50 ~127.15 Mb. In total, 27,753 protein-coding genes 896.31 Mb repetitive sequences in were annotated. undergone WGT (whole-genome triplication), recent WGD duplication) occurred after gamma event, which common other species Cactaceae. A total 29,328 intact LTR-RTs (~696.45 Mb) obtained two expanded lineages, Ty1/copia Ty3/gypsy, main drivers genome. high-density map F1 hybrid populations × ‘Dahong’ pitayas monacanthus their parents constructed, 20,872 bin markers identified (56,380 SNPs) 11 linkage groups. More importantly, through transcriptomic WGCNA (weighted gene coexpression network analysis), global view regulatory network, including structural transcription factors involved fruit betalain biosynthesis, presented. Our data present valuable resource facilitating molecular breeding programs shed novel light modulation biosynthesis edible

Language: Английский

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Betalain plant sources, biosynthesis, extraction, stability enhancement methods, bioactivity, and applications

Food Research International, Journal Year: 2021, Volume and Issue: 151, P. 110821 - 110821

Published: Nov. 25, 2021

Language: Английский

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Multi-colored shades of betalains: recent advances in betacyanin chemistry

Natural Product Reports, Journal Year: 2021, Volume and Issue: 38(12), P. 2315 - 2346

Published: Jan. 1, 2021

Covering: 2001 to 2021Betacyanins cover a class of remarkable natural red-violet plant pigments with prospective chemical and biological properties for wide-ranging applications in food, pharmaceuticals, the cosmetic industry. Betacyanins, forming betalain pigment group together yellow betaxanthins, have gained much attention due increasing social awareness positive impact products on human health. Betalains are commercially recognized as food colorants preliminarily ascertained, but be further investigated, health-promoting properties. In addition, they exhibit structural diversity based glycosylated acylated varieties. The main research directions focused their structure elucidation, methods separation analysis, activities, bioavailability, factors affecting stability, industrial plant-based colorants, drugs, cosmetics well high-yield production stabilization. This review covers period last two decades betacyanin research. first part review, we present an updated classification all known betacyanins derivatives identified by means mass spectrometric NMR techniques. second part, current reports (decarboxylation, oxidation, conjugation, chlorination reactions acyl migration phenomenon) describe semi-synthesis artificial fluorescent betalamic acid conjugates, showing various directions.

Language: Английский

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Major Flower Pigments Originate Different Colour Signals to Pollinators

Frontiers in Ecology and Evolution, Journal Year: 2021, Volume and Issue: 9

Published: Oct. 5, 2021

Flower colour is mainly due to the presence and type of pigments. Pollinator preferences impose selection on flower that ultimately acts Knowing how pollinators perceive flowers with different pigments becomes crucial for a comprehensive understanding plant-pollinator communication evolution. Based space models, we studied whether main groups pollinators, specifically hymenopterans, dipterans, lepidopterans birds, differentially colours generated by major pigment groups. We obtain reflectance data conspicuousness containing one more frequent in flowers: chlorophylls, carotenoids flavonoids. Flavonoids were subsequently classified UV-absorbing flavonoids, aurones-chalcones anthocyanins cyanidin, pelargonidin, delphinidin, malvidin derivatives. found loci carotenoids, aurones-chalcones, occupied regions models these pollinators. The four produced unique cluster loci. Interestingly, differences among almost similar bee, fly, butterfly, bird visual models. Aurones-chalcones showed highest chromatic contrast values, displayed intermediate flavonoids presented lowest values. In model bees, (i.e., white flowers) achromatic contrasts. Ours findings suggest spite omnipresence floral angiosperms, generates higher functional

Language: Английский

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