Multifunctional Fucoidan-loaded Zn-MOF-Encapsulated Microneedles for MRSA-Infected Wound Healing DOI Creative Commons

Zichao Jiang,

Jingyi Li, Jiahao Wang

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Aug. 23, 2023

Abstract Infected wound healing remains a serious challenge in clinical practice due to I) infection by diverse pathogens with drug resistance; II) long-term inflammation of lesions, which inhibits tissue regeneration; and III) that can survive intracellularly evade antibiotic treatment. Microneedle patches as dressings antibacterial activity regeneration capacity are desirable for healing. Herein, we used Zn-MOF (ZIF) loaded low molecular weight fucoidan (Fu) further coated hyaluronic acid (HA) biomedical agent fabricate microneedle patch (HAZ@Fu MN) realize transdermal delivery, intracellular extracellular combination therapy MRSA-infected wounds. Multifunctional HAZ@Fu NPs mixed photocrosslinked gelatin methacryloyl (GelMA) then into the tips MN patch. Enabling reach deep layer dermis, targeting surviving MRSA at subcellular level, is facilitated HA coating. The released Zn 2+ Fu damage bacterial capsule, also conducive regeneration. It has been demonstrated MN, benefiting from these features, dramatically inhibit accelerate epithelial regeneration, resolution neovascularization. therapeutic effects were full-thickness cutaneous wounds mouse model. These results indicate degradable great value promoting infected

Language: Английский

Microneedles’ Device: Design, Fabrication, and Applications DOI Creative Commons
Cristiana Oliveira, J. A. Teixeira, Nélson Oliveira

et al.

Macromol—A Journal of Macromolecular Research, Journal Year: 2024, Volume and Issue: 4(2), P. 320 - 355

Published: May 15, 2024

The delivery of therapeutical molecules through the skin, particularly to its deeper layers, is impaired due stratum corneum layer, which acts as a barrier foreign substances. Thus, for past years, scientists have focused on development more efficient methods deliver skin distinct layers. Microneedles, new class biomedical devices, consist an array microscale needles. This particular device has been drawing attention ability breach corneum, forming micro-conduits facilitate passage molecules. microneedle several advantages over conventional methods, such better medication adherence, easiness, and painless self-administration. Moreover, it possible swiftly or time. Microneedles can vary in shape, size, composition. design process must take into account factors, like location delivery, material, manufacturing process. used large number fields from drug vaccine application cosmetics, therapy, diagnoses, tissue engineering, sample extraction, cancer research, wound healing, among others.

Language: Английский

Citations

25
Multifunctional fucoidan-loaded Zn-MOF-encapsulated microneedles for MRSA-infected wound healing DOI Creative Commons

Zichao Jiang,

Jingyi Li, Jiahao Wang

et al.

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: April 4, 2024

Abstract Infected wound healing remains a challenging task in clinical practice due to several factors: (I) drug-resistant infections caused by various pathogens, (II) persistent inflammation that hinders tissue regeneration and (III) the ability of pathogens persist intracellularly evade antibiotic treatment. Microneedle patches (MNs), recognized for their effecacious painless subcutaneous drug delivery, could greatly enhance if integrated with antibacterial functionality regenerative potential. A multifunctional agent subcellular targeting capability contained novel components, upon loading onto MNs, yield excellent therapeutic effects on infections. In this study, we sythesised zeolitic imidazolate framework-8 nanoparticles (ZIF-8 NPs) loaded low molecular weight fucoidan (Fu) further coating hyaluronic acid (HA), obtained HAZ@Fu NPs, which Methicillin-resistant Staphylococcus aureus (MRSA) growth promotes M2 polarization macrophages. We mixed NPs photocrosslinked gelatin methacryloyl (GelMA) it into tips MNs (HAZ@Fu MNs), administered mice model MRSA-infected full-thickness cutaneous wounds. are able penetrate skin barrier, delivering dermal layer. Since cells within infected tissues extensively express HA receptor CD44, also confirmed endows target MRSA level. vitro vivo murine studies have demonstrated capable deep layers. And facilitated coating, surviving at The effective such as zinc ions, Fu, sustainably released, contributes activity, mitigates inflammation, epithelial fosters neovascularization. Through RNA sequencing macrophages post co-culture HAZ@Fu, Kyoto Encyclopedia Genes Genomes (KEGG) pathway analysis reveals biological functionalities associated potentially be through PI3K-Akt pathway. results indicate synergistic application biodegradable may serve significant adjunct treatment intricate mechanisms driving its merit investigation.

Language: Английский

Citations

17
Highly Active Frozen Nanovesicles Microneedles for Senile Wound Healing via Antibacteria, Immunotherapy, and Skin Regeneration DOI
Jingyi Xu, Sihan Lin, Hongyan Chen

et al.

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: 13(12)

Published: Jan. 23, 2024

Abstract Senile wound healing risks a variety of health complications and makes both economic psychological burdens on patients greatly. Poor activity aged dermal fibroblasts (A‐FBs) local disordered immunoreaction in the deep dermis contribute to delayed healing. Therefore, locally complex microenvironment requires additional processing. Herein, novel double‐layer hyaluronic acid methacrylate (HAMA)/polyvinyl alcohol (PVA) microneedle patch (MNP) coated by young fibroblast‐derived exosomes (Y‐EXOs) (Y‐EXOs@HAMA/PVA MNP) is presented for drug delivery, immunoregulation. A spraying freeze‐drying method applied keeping bioactivity nanovesicles. An ideal loading Y‐EXOs enhanced strength penetration have realized after circulation times. The Y‐EXOs@HAMA/PVA MNP shows an excellent influence skin with active A‐FBs, more deposition collagen less production IL‐17A compared application (A‐EXOs). Moreover, content microRNAs A‐EXOs are sequenced further study. This study initiatively demonstrates that effective function anti‐aging anti‐inflammation expected as strategy delivery promoting hard future clinical application.

Language: Английский

Citations

13
Microneedle patches: the next frontier in cardiovascular care DOI
Simran Kaur,

Shweta Choudhary,

Santimoy Sen

et al.

Drug Delivery and Translational Research, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 29, 2025

Language: Английский

Citations

1
Novel microneedle platforms for the treatment of wounds by drug delivery: A review DOI
Heng An, Zhen Gu, Zhe Huang

et al.

Colloids and Surfaces B Biointerfaces, Journal Year: 2023, Volume and Issue: 233, P. 113636 - 113636

Published: Nov. 11, 2023

Language: Английский

Citations

20
Needle scattered light guided chiplets-interfaced with AI for advanced biomedical application DOI
Bakr Ahmed Taha,

Ehsan M. Abbas,

Ahmed C. Kadhim

et al.

Microelectronic Engineering, Journal Year: 2024, Volume and Issue: 292, P. 112228 - 112228

Published: June 25, 2024

Language: Английский

Citations

8
Spiderweb‐Shaped Iron‐Coordinated Polymeric Network as the Novel Coating on Microneedles for Transdermal Drug Delivery Against Infectious Wounds DOI

Xinwei Fu,

Tao Zhang,

Chuanlan Xia

et al.

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: unknown

Published: June 12, 2024

Abstract Coated microneedles (CMNs) are a minimally invasive platform for immediate‐release transdermal drug delivery. However, the practical applications of CMNs have been significantly hindered by challenges associated with complex formulations, single function, and limited loading capacity. This study has developed spiderweb‐shaped iron‐coordinated polymeric nanowire network (Fe‐IDA NWs). The resulting Fe‐IDA NWs endowed certain viscosity due to synergy multiple supramolecular interactions. allows them replace traditional thickeners as microneedle coatings. NWs‐coated MNs) display rapid disintegration in skin model, which also enables swift diffusion their payloads into deeper layers. Additionally, MNs exhibit desirable enzymatic activity potential antibacterial ability. Thus, can enhance therapeutic efficacy against wound infection through synergistic effects, avoid overly complicated formulation release nontherapeutic molecules conventional CMNs. As proof‐of‐concept, loaded chlorin e6 showed chemodynamic‐photodynamic effect methicillin‐resistant Staphylococcus aureus‐infected model mice. Collectively, this work significant implications future CMNs‐based delivery systems expands application fields metal coordination polymer (MCP) materials.

Language: Английский

Citations

6
Engineered Microneedle System Enables the Smart Regulation of Nanodynamic Sterilization and Tissue Regeneration for Wound Management DOI Creative Commons
Shiyang Lin, Zhongqi Cui,

Qingqiong Luo

et al.

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 13, 2025

Abstract The healing of bacterial biofilm‐infected wounds is a complex process, and the construction emerging therapeutic modalities that regulate microenvironment to magnify effects reduce biotoxicity still highly challenging. Herein, an engineered microneedle (MN) patch reported mediate efficient delivery black phosphorus nanosheets (BP NSs) copper peroxide nanodots (CP NDs) for dual nanodynamic sterilization methicillin‐resistant staphylococcus aureus (MRSA)‐infected wound healing. Results demonstrate system can eliminate biofilm, cytotoxicity, promote angiogenesis tissue regeneration by multiple advantages chemodynamic therapy (CDT), enhanced photodynamic (PDT), improved degradation process from BP NSs phosphate promoting cell proliferation. Notably, balance between excellent stability rapid degradability maintained, mechanism vividly elucidated density functional theory (DFT)‐based molecular dynamics (MD) calculations. Furthermore, transcriptional changes treated MRSA‐infected skin are studied using RNA‐seq technology reveal potential mechanism. As envisaged, proposed MN provides safe, easy, also effective approach achieve temporal regulation wounds.

Language: Английский

Citations

0
Remote Continuous Microinjury-Triggered Cytokines Facilitate Severe Diabetic Foot Ulcer Healing via the Ras/Raf/MEK/ERK Pathway DOI Creative Commons

Xiajie Huang,

Jie Liu, Xiaomei Wu

et al.

Journal of Inflammation Research, Journal Year: 2025, Volume and Issue: Volume 18, P. 1755 - 1772

Published: Feb. 1, 2025

Purpose: Microinjury can trigger in situ tissue repair. Bone transport consists of continuous microinjuries/microfracture and induces bone formation angiogenesis. Tibial cortex transverse (TTT) was found to promote angiogenesis at the foot healing diabetic ulcers (DFUs). However, underlying mechanism remains largely unknown. Methods: We divided 72 Sprague-Dawley rats with DFUs into control, sham, TTT groups. Wound measurement histology were performed evaluate wound processes. Enzyme-linked immunosorbent assay, flow cytometry, immunohistochemistry, Western Blot used assess activity endothelial progenitor cells (EPCs) Ras/Raf/MEK/ERK signaling pathway. Results: accelerated healing, improved epidermal continuity, increased dermal thickness group than control sham Higher levels serum TGF-β 1, PDGF-BB, VEGF detected group. These changes parallel expression wounds frequency EPCs both marrow peripheral circulation, which implied that secreted proliferation migration wounds. The CD31 + cells, SMA-α pathway higher Conclusion: findings showed enhanced production growth factors turn activated EPC through pathway, ultimately contributing DFU healing. Based on these findings, we proposed a theory remote microinjuries repair target tissues (ie, microinjury-induced repair, MIRR). Future studies are needed validate this theory. Keywords: microinjury, transport, tibial ulcer,

Language: Английский

Citations

0
Dissolving Microneedles as In Situ Chemical Reaction Chambers: from Design Strategies to Versatile Biomedical Applications DOI Open Access
Yu Tian, Lili Xia, Xinran Song

et al.

Advanced Functional Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 10, 2025

Abstract The skin microenvironment is a highly intricate and dynamic system, characterized by an acidic pH, diverse microbiota, various metabolites, numerous enzymes, creating both challenges opportunities for the development of innovative drug delivery systems. Dissolving Microneedles (MNs) have emerged as promising, pain‐free alternative to conventional invasive injections, offering ability deliver therapeutics through gradual degradation within skin's interstitial fluids. Building upon unique properties dissolving MNs, novel concept introduced wherein MNs serve in situ chemical reaction chambers. In this framework, can reactants or catalysts skin, enabling initiation specific reactions, such prodrug activation targeted therapy, harmful enhanced synthesis beneficial molecules. Moreover, review systematically explores potential chambers, discussing key aspects their sustained release mechanisms, design strategies, range therapeutic applications. Finally, forward‐looking perspective provided on future addressing broader clinical translation application personalized medicine.

Language: Английский

Citations

0