PeerJ,
Journal Year:
2024,
Volume and Issue:
12, P. e17143 - e17143
Published: April 10, 2024
Phage
display
technology
has
become
an
important
research
tool
in
biological
research,
fundamentally
changing
the
traditional
monoclonal
antibody
preparation
process,
and
been
widely
used
establishment
of
antigen-antibody
libraries,
drug
design,
vaccine
pathogen
detection,
gene
therapy,
antigenic
epitope
cellular
signal
transduction
research.The
phage
is
a
powerful
platform
for
development.
Using
technology,
single
chain
fragment
variable
(scFv)
can
be
screened,
replacing
disadvantage
large
size
antibodies.
libraries
have
significant
implications.
Here
we
describe
types
antibodies,
including
chimeric
bispecific
scFvs.
In
addition,
system,
screening
specific
antibodies
by
application
libraries.
ACS Nano,
Journal Year:
2024,
Volume and Issue:
18(19), P. 12194 - 12209
Published: May 1, 2024
In
situ
vaccines
(ISVs)
utilize
the
localized
delivery
of
chemotherapeutic
agents
or
radiotherapy
to
stimulate
release
endogenous
antigens
from
tumors,
thereby
eliciting
systemic
and
persistent
immune
activation.
Recently,
a
bioinspired
ISV
strategy
has
attracted
tremendous
attention
due
its
features
such
as
an
adjuvant
effect
genetic
plasticity.
M13
bacteriophages
are
natural
nanomaterials
with
intrinsic
immunogenicity,
flexibility,
cost-effectiveness
for
large-scale
production,
demonstrating
potential
application
in
cancer
vaccines.
this
study,
we
propose
based
on
engineered
bacteriophage
targeting
CD40
(M13CD40)
dendritic
cell
(DC)-targeted
stimulation,
named
H-GM-M13CD40.
We
induce
immunogenic
death
tumor
through
local
(S)-10-hydroxycamptothecin
(HCPT),
followed
by
intratumoral
injection
granulocyte-macrophage
colony
stimulating
factor
(GM-CSF)
M13CD40
enhance
DC
recruitment
demonstrate
that
can
result
significant
accumulation
activation
DCs
at
site,
reversing
immunosuppressive
microenvironment.
addition,
H-GM-M13CD40
synergize
PD-1
blockade
abscopal
effects
cold
models.
Overall,
our
study
verifies
immunogenicity
provides
proof
concept
phage
function
ISVs.
Journal of Nanobiotechnology,
Journal Year:
2024,
Volume and Issue:
22(1)
Published: June 25, 2024
Abstract
Bacteriophages
(phages)
represent
a
unique
category
of
viruses
with
remarkable
ability
to
selectively
infect
host
bacteria,
characterized
by
their
assembly
from
proteins
and
nucleic
acids.
Leveraging
exceptional
biological
properties
modifiable
characteristics,
phages
emerge
as
innovative,
safe,
efficient
delivery
vectors.
The
potential
drawbacks
associated
conventional
nanocarriers
in
the
realms
drug
gene
include
lack
cell-specific
targeting,
cytotoxicity,
diminished
vivo
transfection
efficiency.
In
contrast,
engineered
phages,
when
employed
cargo
vectors,
hold
promise
surmount
these
limitations
attain
enhanced
efficacy.
This
review
comprehensively
outlines
current
strategies
for
engineering
delineates
principal
types
utilized
delivery,
explores
application
phage-based
systems
disease
therapy.
Additionally,
an
incisive
analysis
is
provided,
critically
examining
challenges
confronted
within
domain
nanotechnology.
primary
objective
this
article
furnish
theoretical
reference
that
contributes
reasoned
design
development
potent
systems.
Graphical
abstract
Journal of Cellular and Molecular Medicine,
Journal Year:
2024,
Volume and Issue:
28(11)
Published: June 1, 2024
Abstract
Nowadays,
the
use
of
biological
methods
synthesis
nanoparticles
as
substitutes
for
that
high
energy
and
consumption
expensive
dangerous
materials
is
interest
to
researchers
all
over
world.
Biological
synthesising
metal
are
very
important
because
they
easy,
affordable,
safe,
environmentally
friendly
able
control
size
shape
nanoparticles.
One
today
bacteriophages
most
abundant
organisms
in
nature
Nanomaterials
biosynthesized
from
phages
have
shown
various
clinical
applications,
including
antimicrobial
activities,
biomedical
sensors,
drug
gene
delivery
systems,
cancer
treatment
tissue
regeneration.
Therefore,
purpose
this
review
investigate
biosynthesis
with
their
applications.
ACS Nano,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 11, 2025
Although
personalized
neoantigen
cancer
vaccines
have
emerged
as
a
promising
strategy
for
treatment,
challenges
remain
to
develop
immune-stimulatory
carriers
which
allow
simultaneous
transport
of
adjuvants
and
lymph
nodes
(LNs).
With
inherent
immunogenicity,
genetic
plasticity,
efficiency
large-scale
production,
M13
phages
represent
an
attractive
platform
vaccine
delivery
natural
bionanomaterials.
Here,
we
report
the
discovery
anti-CD40
designed
ankyrin
repeat
protein
(DARPin)
propose
bifunctional
ph
age
based
on
this
DARPin
(M13CD40).
M13CD40-based
show
improved
accumulation
prolonged
antigen
retention
in
LNs
compared
with
nontargeting
phage
due
abundance
CD40-positive
cells
LNs.
Besides
intrinsic
immunogenicity
phages,
also
benefit
from
additional
CD40
stimulation
multiple
copies
DARPins
displayed
M13CD40
phages.
Subcutaneous
immunization
results
more
robust
antigen-specific
immune
responses
superior
antitumor
efficacy
poorly
immunogenic
tumor
models
vaccines.
Combination
therapy
PD-1
blockade
further
enhances
T
cell
cytotoxicity
improves
control.
To
summarize,
our
findings
highlight
nanoagonist
well
efficient
vehicle
LN-targeted
Advanced Materials,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Sept. 5, 2024
Abstract
Photodynamic
therapy
(PDT)
eliminates
cancer
cells
by
converting
endogenous
oxygen
into
reactive
species
(ROS).
However,
its
efficacy
is
significantly
hindered
hypoxia
in
solid
tumors.
Hence,
to
engineer
filamentous
fd
phage,
a
human‐friendly
bacteria‐specific
virus
proposed,
nanozyme‐nucleating
photosensitizer‐loaded
tumor‐homing
nanofiber
for
enhanced
production
of
ROS
hypoxic
tumor.
Specifically,
Pt‐binding
and
peptides
are
genetically
displayed
on
the
sidewall
tip
respectively.
The
induced
nucleation
orientation
Pt
nanozymes
(PtNEs)
phage.
resultant
PtNE‐coated
phage
exhibits
sustained
catalytic
conversion
hydrogen
peroxide
tumors
O
2
producing
needed
PDT,
compared
non‐phage‐templated
PtNE.
Density
functional
theory
(DFT)
calculations
verify
mechanism
phage‐templated
After
intravenous
injection
indocyanine
green
(ICG)‐loaded
phages
breast
tumor‐bearing
mice,
nanofibers
home
effectively
inhibit
tumor
growth
PtNE‐enhanced
PDT.
can
also
serve
as
imaging
probe
due
fluorescence
ICG.
This
work
demonstrates
that
engineered
become
tumor‐hypoxia‐relieving
nanofibers,
act
cancer‐targeting
with
improved
performance
effective
targeted
International Journal of Molecular Sciences,
Journal Year:
2023,
Volume and Issue:
24(23), P. 17029 - 17029
Published: Dec. 1, 2023
RNA
and
single-stranded
DNA
(ssDNA)
phages
make
up
an
understudied
subset
of
bacteriophages
that
have
been
rapidly
expanding
in
the
last
decade
thanks
to
advancements
metaviromics.
Since
their
discovery,
applications
genetic
engineering
ssDNA
revealed
immense
potential
for
diverse
healthcare
biotechnology.
In
this
review,
we
explore
past
present
underexplored
group
phages,
particularly
current
usage
as
therapeutic
agents
against
multidrug-resistant
bacteria.
We
also
discuss
techniques
such
recombinant
expression,
CRISPR/Cas-based
genome
editing,
synthetic
rebooting
phage-like
particles
role
tailoring
disease
treatment,
imaging,
biomaterial
development,
delivery
systems.
Recent
breakthroughs
phage
are
especially
highlighted.
conclude
with
a
perspective
on
challenges
future
prospects,
emphasizing
untapped
diversity
revolutionize
biotechnology
medicine.
Nature Communications,
Journal Year:
2024,
Volume and Issue:
15(1)
Published: July 11, 2024
Abstract
The
effective
isolation
of
rare
target
cells,
such
as
circulating
tumor
from
whole
blood
is
still
challenging
due
to
the
lack
a
capturing
surface
with
strong
target-binding
affinity
and
non-target-cell
resistance.
Here
we
present
solution
leveraging
flexibility
bacterial
virus
(phage)
nanofibers
their
sidewalls
displaying
cell-specific
aptamers
ends
tethered
magnetic
beads.
Such
flexible
phages,
low
stiffness
Young’s
modulus,
can
twist
adapt
recognize
cell
receptors,
energetically
enhancing
entropically
discouraging
non-target
cells
(white
cells)
adsorption.
beads
phages
isolate
count
significant
increase
in
reduction
absorption
compared
having
rigid
phages.
This
differentiates
breast
cancer
patients
healthy
donors,
impressive
area
under
curve
(0.991)
at
optimal
detection
threshold
(>4
mL
−1
).
Immunostaining
captured
precisely
determines
subtypes
diagnostic
accuracy
91.07%.
Our
study
reveals
power
viral
mechanical
attributes
designing
surfaces
superior
binding
anti-fouling.
Pathogens,
Journal Year:
2024,
Volume and Issue:
13(2), P. 152 - 152
Published: Feb. 7, 2024
Humans
have
used
fermentation
processes
since
the
Neolithic
period,
mainly
to
produce
beverages.
The
turning
point
occurred
in
1850s,
when
Louis
Pasteur
discovered
that
resulted
from
metabolism
of
living
microorganisms.
This
discovery
led
fast
development
fermented
food
production.
importance
industrial
based
on
significantly
increased.
Many
branches
industry
rely
metabolisms
bacteria,
for
example,
dairy
(cheese,
milk,
yogurts),
pharmaceutical
(insulin,
vaccines,
antibiotics),
or
production
chemicals
(acetone,
butanol,
acetic
acid).
These
are
mass
involving
a
large
financial
outlay.
That
is
why
it
essential
minimize
threats
One
major
threat
affecting
bacteria-based
bacteriophage
infections,
causing
substantial
economic
losses.
first
reported
phage
infections
appeared
1930s,
and
companies
still
struggle
fight
against
phages.
review
shows
cases
most
common
methods
prevent
infections.
Life,
Journal Year:
2024,
Volume and Issue:
14(5), P. 600 - 600
Published: May 8, 2024
Most
of
the
annual
10
million
cancer-related
deaths
are
caused
by
metastatic
disease.
Survival
rates
for
cancer
strongly
dependent
on
type
and
its
stage
at
detection.
Early
detection
remains
a
challenge
due
to
lack
reliable
biomarkers
cost-efficient
screening
methods.
Phage
biosensors
can
offer
solution
early
using
non-invasive
liquid
biopsies.
Here,
we
report
first
results
bifunctional
phage
biosensor
detect
urological
cancers
from
urine.
A
dye-sensitized
library
was
used
select
metastasis-related
binders.
After
selection
rounds,
most
promising
candidate
classify
controls.
Additionally,
applied
one
chemical
sensor
(phenoxazine
non-fluorescent
dye)
statistical
significance
(
