Filamented Hydrogels as Tunable Conduits for Guiding Neurite Outgrowth

Materials Today Bio, Journal Year: 2025, Volume and Issue: 31, P. 101471 - 101471

Published: Jan. 11, 2025

Anisotropic scaffolds with unidirectionally aligned fibers present an optimal solution for nerve tissue engineering and graft repair. This study investigates the application of filamented light (FLight) biofabrication to create hydrogel matrices featuring highly microfilaments, facilitating neurite guidance outgrowth from encapsulated chicken dorsal root ganglion (DRG) cells. FLight employs optical modulation instability (OMI) rapidly safely (<5 s) fabricate constructs precise microfilament alignment. The tunability was demonstrated by adjusting four key parameters: stiffness, porosity, growth factor release, incorporation biological cues. Matrix stiffness fine-tuned varying projection dose, yielding ranging 0.6 5.7 kPa. Optimal occurred at a kPa, achieving 2.5 mm over 4 days. porosity modified using diffraction gratings in setup. While significant differences alignment were observed between bulk gels, further increases 40 % 70 enhanced cell migration axon bundling without significantly affecting maximal outgrowth. protein microcrystals containing (NGF) into photoresin enabled sustained need additional NGF media. Finally, laminin added resin enhance bioactivity biomaterial, resulting increase maximum 3.5 after days culture softer matrices. Overall, varied matrix properties achieved through outgrowth, highlighting importance adaptable scaffold characteristics guiding development. demonstrates potential as versatile platform creating ideal clinical applications repair engineering.

Language: Английский

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Growth factors: Bioactive macromolecular drugs for peripheral nerve injury treatment – Molecular mechanisms and delivery platforms

Biomedicine & Pharmacotherapy, Journal Year: 2023, Volume and Issue: 170, P. 116024 - 116024

Published: Dec. 19, 2023

Bioactive macromolecular drugs known as Growth Factors (GFs), approved by the Food and Drug Administration (FDA), have found successful application in clinical practice. They hold significant promise for addressing peripheral nerve injuries (PNIs). Peripheral guidance conduits (NGCs) loaded with GFs, context of tissue engineering, can ensure sustained efficient release these bioactive compounds. This, turn, maintains a stable, long-term, effective GF concentration essential treating damaged nerves. regeneration is complex process that entails secretion various GFs. Following PNI, GFs play pivotal role promoting cell growth survival, axon myelin sheath regeneration, differentiation, angiogenesis. also regulate regenerative microenvironment, stimulate plasticity changes post-nerve injury, and, consequently, expedite structure function repair. Both exogenous endogenous including NGF, BDNF, NT-3, GDNF, IGF-1, bFGF, VEGF, been successfully onto NGCs using techniques like physical adsorption, blend doping, chemical covalent binding, engineered transfection. These approaches effectively promoted repair Numerous studies demonstrated similar functional therapeutic outcomes compared to autologous transplantation. This evidence underscores substantial potential domain In this article, we provide an overview drug delivery systems utilizing NGCs. Looking ahead, commercial materials facilitate nerves maintain functionality distant target organs through

Language: Английский

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Permeable Bioelectronics toward Biointegrated Systems

Chemical Reviews, Journal Year: 2024, Volume and Issue: 124(10), P. 6543 - 6591

Published: May 10, 2024

Bioelectronics integrates electronics with biological organs, sustaining the natural functions of organs. Organs dynamically interact external environment, managing internal equilibrium and responding to stimuli. These interactions are crucial for maintaining homeostasis. Additionally, organs possess a soft stretchable nature; encountering objects differing properties can disrupt their function. Therefore, when electronic devices come into contact objects, permeability these devices, enabling substance exchanges mechanical compliance inherent functionality This review discusses recent advancements in permeable bioelectronics, emphasizing materials, structures, wide range applications. The also addresses current challenges potential solutions, providing insights integration

Language: Английский

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Injectable Hydrogels for Nervous Tissue Repair—A Brief Review

Gels, Journal Year: 2024, Volume and Issue: 10(3), P. 190 - 190

Published: March 9, 2024

The repair of nervous tissue is a critical research field in engineering because the degenerative process injured system. In this review, we summarize progress injectable hydrogels using vitro and vivo studies for regeneration tissue. Traditional treatments have not been favorable patients, as they are invasive inefficient; therefore, promising treatment damaged This review will contribute to better understanding potential scaffolds drug delivery system neural applications.

Language: Английский

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HIF-1α Induced by Hypoxia Promotes Peripheral Nerve Injury Recovery Through Regulating Ferroptosis in DRG Neuron

Molecular Neurobiology, Journal Year: 2024, Volume and Issue: 61(9), P. 6300 - 6311

Published: Jan. 31, 2024

Language: Английский

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Hydrogel-Based Innovations in Carpal Tunnel Syndrome: Bridging Pathophysiological Complexities and Translational Therapeutic Gaps

Gels, Journal Year: 2025, Volume and Issue: 11(1), P. 52 - 52

Published: Jan. 9, 2025

Carpal Tunnel Syndrome (CTS) is a prevalent neuropathic disorder caused by chronic compression of the median nerve, leading to sensory and motor impairments. Conventional treatments, such as corticosteroid injections, wrist splinting, surgical decompression, often fail provide adequate outcomes for or recurrent cases, emphasizing need innovative therapies. Hydrogels, highly biocompatible three-dimensional biomaterials with customizable properties, hold significant potential CTS management. Their ability mimic extracellular matrix facilitates localized drug delivery, anti-adhesion barrier formation, tissue regeneration. Advances in hydrogel engineering have introduced stimuli-responsive systems tailored biomechanical environment carpal tunnel, enabling sustained therapeutic release improved integration. Despite these promising developments, applications remain underexplored. Key challenges include absence CTS-specific preclinical models rigorous clinical validation. Addressing gaps could unlock full hydrogel-based interventions, which offer minimally invasive, solutions that improve long-term reduce recurrence rates. This review highlights hydrogels transformative approach therapy, advocating continued research address translational barriers. These innovations redefine treatment landscape, significantly enhancing patient care quality life.

Language: Английский

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Hydrogels for Peripheral Nerve Repair: Emerging Materials and Therapeutic Applications

Gels, Journal Year: 2025, Volume and Issue: 11(2), P. 126 - 126

Published: Feb. 9, 2025

Peripheral nerve injuries pose a significant clinical challenge due to the complex biological processes involved in repair and their limited regenerative capacity. Despite advances surgical techniques, conventional treatments, such as autografts, are faced with limitations like donor site morbidity inconsistent functional outcomes. As such, there is growing interest new, novel, innovative strategies enhance regeneration. Tissue engineering/regenerative medicine its use of biomaterials an emerging example strategy. Within realm tissue engineering, functionalized hydrogels have gained considerable attention ability mimic extracellular matrix, support cell growth differentiation, even deliver bioactive molecules that can promote repair. These be engineered incorporate factors, peptides, stem cells, creating conducive microenvironment for cellular axonal Recent advancements materials well biology led development sophisticated hydrogel systems, not only provide structural support, but also actively modulate inflammation, recruitment, stimulate neurogenesis. This review explores potential peripheral repair, highlighting composition, biofunctionalization, mechanisms action. A comprehensive analysis preclinical studies provides insights into efficacy these promoting growth, neuronal survival, regeneration, and, ultimately, recovery. Thus, this aims illuminate promise transformative tool field bridging gap between complexity feasibility.

Language: Английский

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Electroactive Polymers for On‐Demand Drug Release

Advanced Healthcare Materials, Journal Year: 2023, Volume and Issue: 13(3)

Published: Oct. 20, 2023

Conductive materials have played a significant role in advancing society into the digital era. Such are able to harness power of electricity and used control many aspects daily life. polymers (CPs) an emerging group that possess metal-like conductivity yet retain desirable polymeric features, such as processability, mechanical properties, biodegradability. Upon receiving electrical stimulus, CPs can be tailored achieve number responses, harvesting energy stimulating tissue growth. The recent FDA approval CP-based material for medical device has invigorated their research healthcare. In drug delivery, act switches, release is achieved at flick switch, thereby providing unprecedented over release. this review, developments CP electroactive voltage-stimuli responsive delivery systems evaluated. review demonstrates distinct profiles by formulations, both precision ease stimuli response. This level dynamism promises yield "smart medicines" warrants further research. concludes outlook on formulations highlighting integral roles healthcare IoT.

Language: Английский

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Advances in Biomimetic Nerve Guidance Conduits for Peripheral Nerve Regeneration

Nanomaterials, Journal Year: 2023, Volume and Issue: 13(18), P. 2528 - 2528

Published: Sept. 10, 2023

Injuries to the peripheral nervous system are a common clinical issue, causing dysfunctions of motor and sensory systems. Surgical interventions such as nerve autografting necessary repair damaged nerves. Even with autografting, i.e., gold standard, malfunctioning mismatches between injured donor nerves often lead unwanted failure. Thus, there is an urgent need for new intervention in practice achieve full functional recovery. Nerve guidance conduits (NGCs), providing physicochemical cues guide neural regeneration, have great potential regeneration Typically, NGCs tubular structures various configurations create microenvironment that induces oriented accelerated growth axons promotes neuron cell migration tissue maturation within tissue. Once native environment better understood, ideal should maximally recapitulate those key physiological attributes regeneration. Indeed, NGC design has evolved from solely physical biochemical stimulation. fabrication requires fundamental considerations distinct structures, associated extracellular compositions (extracellular matrices, factors, cytokines), cellular components, advanced technologies can mimic structure morphology matrices. this review mainly summarizes recent advances state-of-the-art terms biomaterial innovations, structural design, provides in-depth discussion responses (adhesion, spreading, alignment) biomimetic repair.

Language: Английский

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Differentiated mesenchymal stem cells-derived exosomes immobilized in decellularized sciatic nerve hydrogels for peripheral nerve repair

Journal of Controlled Release, Journal Year: 2024, Volume and Issue: 368, P. 24 - 41

Published: Feb. 21, 2024

Language: Английский

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