Challenges and Future Perspectives in Modeling Neurodegenerative Diseases Using Organ‐on‐a‐Chip Technology

Advanced Science, Journal Year: 2024, Volume and Issue: 11(32)

Published: June 23, 2024

Abstract Neurodegenerative diseases (NDDs) affect more than 50 million people worldwide, posing a significant global health challenge as well high socioeconomic burden. With aging constituting one of the main risk factors for some NDDs such Alzheimer's disease (AD) and Parkinson's (PD), this societal toll is expected to rise considering predicted increase in population limited progress development effective therapeutics. To address failure rates clinical trials, legislative changes permitting use alternatives traditional pre‐clinical vivo models are implemented. In regard, microphysiological systems (MPS) organ‐on‐a‐chip (OoC) platforms constitute promising tool, due their ability mimic complex human‐specific tissue niches vitro. This review summarizes current modeling using OoC technology discusses five critical aspects still insufficiently addressed date. Taking these into consideration future MPS will advance vitro translational value setting.

Language: Английский

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3D Bioprinting for Engineered Tissue Constructs and Patient‐Specific Models: Current Progress and Prospects in Clinical Applications

Advanced Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 17, 2024

Advancements in bioprinting technology are driving the creation of complex, functional tissue constructs for use engineering and regenerative medicine. Various methods, including extrusion, jetting, light-based bioprinting, have their unique advantages drawbacks. Over years, researchers industry leaders made significant progress enhancing techniques materials, resulting production increasingly sophisticated constructs. Despite this progress, challenges still need to be addressed achieving clinically relevant, human-scale constructs, presenting a hurdle widespread clinical translation. However, with ongoing interdisciplinary research collaboration, field is rapidly evolving holds promise personalized medical interventions. Continued development refinement technologies potential address complex needs, enabling functional, transplantable tissues organs, as well advanced vitro models.

Language: Английский

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Understanding organotropism in cancer metastasis using microphysiological systems

Lab on a Chip, Journal Year: 2024, Volume and Issue: 24(6), P. 1542 - 1556

Published: Jan. 1, 2024

Organotropism is an important concept to explain the process of cancer metastasis. In this paper, we introduce microphysiological systems with simultaneous physiological relevance and high throughput recapitulate series progression.

Language: Английский

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Passive-Flow-Based MPS: Emerging Physiological Flow-Mimetic Platforms for Studying Effects of Flow on Single Tissues and Inter-tissue Interactions

BioChip Journal, Journal Year: 2024, Volume and Issue: 18(2), P. 186 - 210

Published: March 8, 2024

Language: Английский

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Advances in microfluidics for biomedical engineering applications

Elsevier eBooks, Journal Year: 2025, Volume and Issue: unknown, P. 301 - 327

Published: Jan. 1, 2025

Language: Английский

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Treatment Response to Oncolytic Virus in Patient-Derived Breast Cancer and Hypopharyngeal Cancer Organoids: Evaluation via a Microfluidics Organ-on-a-Chip System

Bioengineering, Journal Year: 2025, Volume and Issue: 12(2), P. 146 - 146

Published: Feb. 4, 2025

In this study, we present an oncolytic virus (OV) evaluation system established using microfluidic organ-on-a-chip (OOC) systems and patient-derived organoids (PDOs), which was used in the development of a novel virus, AD4-GHPE. An OV offers advantages such as good targeting ability minimal side effects, it has achieved significant breakthroughs when combined with immunotherapy recent clinical trials. The OVs become emerging research focus. PDOs can preserve heterogeneity situ tumor tissues, whereas OOC automate standardize various experimental procedures. These have been applied cutting-edge drug screening cell therapy experiments; however, their use functionally complex viruses remains to be explored. constructed recombinant adenovirus, AD4-GHPE, evaluated through functional validations hypopharyngeal breast cancer organoids. results confirmed that AD4-GHPE exhibits three antitumor mechanisms, namely, tumor-specific cytotoxicity, reduction programmed death ligand 1 (PD-L1) expression cells increase CD8+ T-cell activity, granulocyte–macrophage colony-stimulating factor (GM-CSF) secretion. combining efficient reliable, providing personalized treatment recommendations for patients offering industrialized standardized ideas OVs.

Language: Английский

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Organotypic models of the mammalian brain for molecular psychiatry and neurology

Vestnik nevrologii psihiatrii i nejrohirurgii (Bulletin of Neurology Psychiatry and Neurosurgery), Journal Year: 2025, Volume and Issue: 1, P. 22 - 32

Published: Jan. 15, 2025

The increasing prevalence of neurological, neurodegenerative and psychiatric diseases makes it necessary to create appropriate informative models study the molecular mechanisms their pathogenesis search for promising approaches treat them. 2D neuron cultures do not display full complexity intercellular interactions in nervous system, brains even primitive mammals are too complex always suitable modeling human brain pathologies. missing link between 2D-cultures vivo organotypic 3D-cultures. Such include surviving slices, self-organizing 3D-cultures (neurospheres, organoids, assembloids) brain-on-a-chip cultures. These 3D-models make possible reproduce only cellular composition brain, but a large extent its architectonics or simulate with other organs tissues within body. Their application has already made obtain data on some involved development hereditary, inflammatory central as well propose test treatment. This literature review discusses possibilities, limitations most striking examples use these models. 3D animal opens unprecedented perspectives neurobiology, psychiatry neurology. high potential flexibility models, need various pathologies striving establish causal disease allow us assert that very near future will experience rapid growth, widespread use.

Language: Английский

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In vitro models of the choroid plexus and the blood-cerebrospinal fluid barrier: advances, applications, and perspectives

Human Cell, Journal Year: 2024, Volume and Issue: 37(5), P. 1235 - 1242

Published: Aug. 5, 2024

The choroid plexus (CP), a highly vascularized endothelial-epithelial convolute, is placed in the ventricular system of brain and produces large part cerebrospinal fluid (CSF). Additionally, CP location blood-CSF barrier (BCSFB) that separates CSF from blood stream endothelium. In vitro models BCSFB are high importance to investigate biological functions BCSFB. Since involved several serious diseases, these promise help researching processes contributing diseases during development treatment options. this review, we provide an overview on available advances have been made toward more sophisticated "in vivo near" systems as organoids microfluidic lab-on-a-chip approaches. We go into applications research objectives for which various modeling can be used discuss possible future prospects perspectives.

Language: Английский

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Full-Thickness Perfused Skin-on-a-Chip with In Vivo-Like Drug Response for Drug and Cosmetics Testing

Bioengineering, Journal Year: 2024, Volume and Issue: 11(11), P. 1055 - 1055

Published: Oct. 23, 2024

In this study, we present a novel 3D perfused skin-on-a-chip model fabricated using micro-precision printing, which offers streamlined and reproducible approach for incorporating perfusion. Perfused skin models are well-regarded their advantages, such as improved nutrient supply, enhanced barrier function, prolonged tissue viability. However, current often require complex setups, self-assembled endothelial cells or sacrificial rods, prone to variability time-consuming. Our uses projection micro-stereolithography printing create precise microcapillary-like channels biocompatible resin, overcoming the drug-absorbing properties of PDMS. A customized chip holder allows simultaneous culture six chips, enabling high-throughput testing. The engineered features distinct dermis epidermis layers, confirmed via H&E staining immunostaining. To evaluate drug screening capabilities, inflammation was induced TNF-α treated with dexamethasone, cytokine levels compared 2D cultures human biopsies. exhibited response trends similar skin, while showing reduced cytotoxicity over time This provides reliable, physiologically relevant alternative cosmetics screening, simplifying perfusion setup preserving key benefits.

Language: Английский

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TPP-Based Microfluidic Chip Design and Fabrication Method for Optimized Nerve Cells Directed Growth

Cyborg and Bionic Systems, Journal Year: 2024, Volume and Issue: 5

Published: Jan. 1, 2024

Microfluidic chips offer high customizability and excellent biocompatibility, holding important promise for the precise control of biological growth at microscale. However, microfluidic employed in studies regulating cell are typically fabricated through 2D photolithography. This approach partially restricts diversity platform designs manufacturing efficiency. paper presents a method designing neural culture (NCMC) using two-photon polymerization (TPP), where discrete directional is optimized studying associated geometric parameters on-chip microchannels. study involves simulations discussions regarding effects different hatching distances on mold surface topography printing time Describe print preview module, which determines appropriate accuracy corresponding to desired structure. With assistance 3D maskless lithography system, micron-level rapid target molds with dimensions were achieved. For NCMC parameters, COMSOL software was used simulate local flow velocity shear stress characteristics within SH-SY5Y cells selected differentiation experiments parameters. The results demonstrate that TPP-based efficiently constructs precision, optimizing growth. We anticipate our will hold great construction application microscale drug models.

Language: Английский

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