A nociceptive neuronal ensemble in the dorsomedial prefrontal cortex underlies pain chronicity DOI Creative Commons
Xuetao Qi, Kun Cui, Yu Zhang

et al.

Cell Reports, Journal Year: 2022, Volume and Issue: 41(11), P. 111833 - 111833

Published: Dec. 1, 2022

Pain chronicity involves unpleasant experience in both somatosensory and affective aspects, accompanied with the prefrontal cortex (PFC) neuroplastic alterations. However, whether specific PFC neuronal ensembles underlie pain remains elusive. Here we identify a nociceptive ensemble dorsomedial (dmPFC), which shows prominent reactivity to stimuli. We observed that this distinct molecular characteristics is densely connected pain-related regions including basolateral amygdala (BLA) lateral parabrachial nuclei (LPB). Prolonged chemogenetic activation of ensemble, but not randomly transfected subset dmPFC neurons, induces chronic pain-like behaviors normal mice. By contrast, silencing neurons relieves hypersensitivity anxiety mice inflammatory pain. These results suggest presence processing information regulating chronicity.

Language: Английский

Correlated Neural Activity and Encoding of Behavior across Brains of Socially Interacting Animals DOI Creative Commons

Lyle Kingsbury,

Shan Huang, Jun Wang

et al.

Cell, Journal Year: 2019, Volume and Issue: 178(2), P. 429 - 446.e16

Published: June 20, 2019

Language: Английский

Citations

319
Circuit Investigations With Open-Source Miniaturized Microscopes: Past, Present and Future DOI Creative Commons
Daniel Aharoni, Tycho M. Hoogland

Frontiers in Cellular Neuroscience, Journal Year: 2019, Volume and Issue: 13

Published: April 5, 2019

The ability to simultaneously image the spatiotemporal activity signatures from many neurons during unrestrained vertebrate behaviors has become possible through development of miniaturized fluorescence microscopes, or miniscopes, sufficiently light be carried by small animals such as bats, birds and rodents. Miniscopes have permitted study circuits underlying song vocalization, action sequencing, head-direction tuning, spatial memory encoding sleep name a few. foundation for these microscopes been laid over last two decades academic research with some this work resulting in commercialization. More recently, open-source initiatives led an even broader adoption miniscopes neuroscience community. Open-source designs allow rapid modification extension their function, which resulted new generation that now permit wire-free wireless recording, concurrent electrophysiology imaging, two-color detection, simultaneous optical actuation read-out well wide-field volumetric light-field imaging. These novel will further expand toolset those seeking affordable methods probe neural circuit function naturalistic behaviors. Here, we discuss early development, present use future potential miniscopes.

Language: Английский

Citations

211
Optical voltage imaging in neurons: moving from technology development to practical tool DOI
Thomas Knöpfel,

Chenchen Song

Nature reviews. Neuroscience, Journal Year: 2019, Volume and Issue: 20(12), P. 719 - 727

Published: Nov. 8, 2019

Language: Английский

Citations

186
Miniature two-photon microscopy for enlarged field-of-view, multi-plane and long-term brain imaging DOI
Weijian Zong, Runlong Wu, Shiyuan Chen

et al.

Nature Methods, Journal Year: 2021, Volume and Issue: 18(1), P. 46 - 49

Published: Jan. 1, 2021

Language: Английский

Citations

171
Ventral hippocampal projections to the medial prefrontal cortex regulate social memory DOI Creative Commons
Mary L. Phillips, Holly Robinson, Lucas Pozzo‐Miller

et al.

eLife, Journal Year: 2019, Volume and Issue: 8

Published: May 21, 2019

Inputs from the ventral hippocampus (vHIP) to medial prefrontal cortex (mPFC) are implicated in several neuropsychiatric disorders. Here, we show that vHIP-mPFC projection is hyperactive Mecp2 knockout mouse model of autism spectrum disorder Rett syndrome, which has deficits social memory. Long-term excitation mPFC-projecting vHIP neurons wild-type mice impaired memory, whereas their long-term inhibition rescued memory deficits. The extent improvement was negatively correlated with vHIP-evoked responses mPFC slices, on a mouse-per-mouse basis. Acute manipulations affected region and behavior selective manner, suggesting proper signaling necessary recall memories. In addition, identified an altered pattern innervation neurons, increased synaptic strength inputs onto layer five pyramidal as contributing factors aberrant mice.

Language: Английский

Citations

170
Aerobic glycolysis is the predominant means of glucose metabolism in neuronal somata, which protects against oxidative damage DOI
Wei Yao,

QianQian Miao,

Qian Zhang

et al.

Nature Neuroscience, Journal Year: 2023, Volume and Issue: 26(12), P. 2081 - 2089

Published: Nov. 23, 2023

Language: Английский

Citations

67
Social circuits and their dysfunction in autism spectrum disorder DOI Creative Commons
Masaaki Sato, Nobuhiro Nakai,

Shuhei Fujima

et al.

Molecular Psychiatry, Journal Year: 2023, Volume and Issue: 28(8), P. 3194 - 3206

Published: Aug. 1, 2023

Social behaviors, how individuals act cooperatively and competitively with conspecifics, are widely seen across species. Rodents display various social many different behavioral paradigms have been used for investigating their neural circuit bases. behavior is highly vulnerable to brain network dysfunction caused by neurological neuropsychiatric conditions such as autism spectrum disorders (ASDs). Studying mouse models of ASD provides a promising avenue toward elucidating mechanisms abnormal potential therapeutic targets treatment. In this review, we outline recent progress key findings on underlying behavior, particular emphasis rodent studies that monitor manipulate the activity specific circuits using modern systems neuroscience approaches. mediated distributed brain-wide among major cortical (e.g., medial prefrontal cortex (mPFC), anterior cingulate cortex, insular (IC)) subcortical nucleus accumbens, basolateral amygdala (BLA), ventral tegmental area) structures, influenced multiple neuromodulatory oxytocin, dopamine, serotonin). We particularly draw special attention IC unique area mediates multisensory integration, encoding ongoing interaction, decision-making, emotion, empathy. Additionally, synthesis demonstrates dysfunctions in mPFC-BLA circuitry neuromodulation prominent. Pharmacological rescues local or systemic oral) administration drugs provided valuable clues developing new agents ASD. Future efforts technological advances will push forward next frontiers field, elucidation inter-brain dynamics during real virtual interactions, establishment circuit-based therapy affecting functions.

Language: Английский

Citations

52
Miniature three-photon microscopy maximized for scattered fluorescence collection DOI
Chunzhu Zhao, Shiyuan Chen, Lifeng Zhang

et al.

Nature Methods, Journal Year: 2023, Volume and Issue: 20(4), P. 617 - 622

Published: Feb. 23, 2023

Language: Английский

Citations

48
A distinct cortical code for socially learned threat DOI
Shana Silverstein,

Ruairi O’Sullivan,

Olena Bukalo

et al.

Nature, Journal Year: 2024, Volume and Issue: 626(8001), P. 1066 - 1072

Published: Feb. 7, 2024

Language: Английский

Citations

18
Transformations in prefrontal ensemble activity underlying rapid threat avoidance learning DOI Creative Commons
Christopher Gabriel, Tanya Gupta,

Asai Sánchez-Fuentes

et al.

Current Biology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

Language: Английский

Citations

2