Tracing Immunological Interaction in Trimethylamine N‐Oxide Hydrogel‐Derived Zwitterionic Microenvironment During Promoted Diabetic Wound Regeneration

Advanced Materials, Journal Year: 2024, Volume and Issue: 36(33)

Published: June 18, 2024

The diabetic wound healing is challenging due to the sabotaged delicate balance of immune regulation via an undetermined pathophysiological mechanism, so it crucial decipher multicellular signatures underlying and seek therapeutic strategies. Here, this work develops a strategy using novel trimethylamine N-oxide (TMAO)-derived zwitterionic hydrogel promote healing, explore multi-cellular ecosystem around hydrogel, mapping out overview different cells in microenvironment by single-cell RNA sequencing. diverse cellular heterogeneity revealed, highlighting critical role macrophage neutrophils managing healing. It found that polyzwitterionic can upregulate Ccl3+ macrophages downregulate S100a9+ facilitate their interactions compared with polyanionic polycationic hydrogels, validating effect on activation adaptive system. Moreover, inhibits formation neutrophil extracellular traps (NETs) promotes angiogenesis, thus improving These findings expand horizons sophisticated orchestration systems zwitterion-directed repair uncover new strategies immunoregulatory biomaterials.

Language: Английский

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Therapeutic Potential of Microneedle Assisted Drug Delivery for Wound Healing: Current State of the Art, Challenges, and Future Perspective

AAPS PharmSciTech, Journal Year: 2025, Volume and Issue: 26(1)

Published: Jan. 8, 2025

Language: Английский

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A differential-targeting core–shell microneedle patch with coordinated and prolonged release of mangiferin and MSC-derived exosomes for scarless skin regeneration

Materials Horizons, Journal Year: 2024, Volume and Issue: 11(11), P. 2667 - 2684

Published: Jan. 1, 2024

Microneedles for skin regeneration are conventionally restricted by uncontrollable multi-drug release, limited types of drugs, and poor wound adhesion. Here, a novel core-shell microneedle patch is developed scarless repair, where the shell composed hydrophilic gelatin methacryloyl (GelMA) loaded with mangiferin, an anti-inflammatory small molecule, core hydrophobic poly (lactide-co-propylene glycol-co-lactide) dimethacrylates (PGLADMA) bioactive macromolecule human mesenchymal stromal cell (hMSC)-derived exosomes. This material choice provides several benefits: GelMA swelling interface tissue interlocking rapid release mangiferin at early healing stage anti-inflammation, whereas PGLADMA offers long-term encapsulation exosomes (30% in 3 weeks), promoting sustained angiogenesis anti-inflammation. Our results demonstrate that possesses properties can induce both vitro terms macrophage polarization tube formation umbilical vein endothelial cells (HUVECs), vivo re-epithelization, vessel formation. Importantly, we also observe reduced scar vivo. Altogether, degradation dynamics our hydrophilic/hydrophobic materials enable design differential prolonged regeneration, potential other therapies exosome release.

Language: Английский

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Fabrication of amino-capped Pluronic F127 with aldehyde dextran chains: A strategy improving extensibility, compressibility and self-healing hydrogel for wound healing

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 304, P. 140774 - 140774

Published: Feb. 10, 2025

Language: Английский

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The Combination of Bioactive Herbal Compounds with Biomaterials for Regenerative Medicine

Tissue Engineering Part B Reviews, Journal Year: 2024, Volume and Issue: 30(6), P. 607 - 630

Published: March 14, 2024

Regenerative medicine aims to restore the function of diseased or damaged tissues and organs by cell therapy, gene tissue engineering, along with adjunctive application bioactive molecules. Traditional molecules such as growth factors cytokines have shown great potential in regulation cellular behavior, but disadvantages limited source, high cost, short half-life, side effects. In recent years, herbal compounds extracted from natural plants/herbs gained increasing attention. This is not only because are easily obtained, inexpensive, mostly safe, reliable, also owing their excellent effects including anti-inflammatory, antibacterial, antioxidative, proangiogenic behavior ability promote stem differentiation. Such play important roles processes related regeneration. Furthermore, moieties can form physical chemical bonds scaffolds, which contributes improved mechanical strength stability scaffolds. Thus, incorporation biomaterials a promising direction for future regenerative applications. Herein, an overview on use combined different biomaterial scaffolds presented. We firstly introduce classification, structures, properties components then provide comprehensive survey engineer repair/regeneration skin, cartilage, bone, neural, heart tissues. Finally, we highlight challenges prospects development toward clinical translation. Overall, it believed that combination could be perspective next generation medicine.

Language: Английский

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Quercetin loaded polymeric dissolving microarray patches: fabrication, characterisation and evaluation

Drug Delivery and Translational Research, Journal Year: 2024, Volume and Issue: unknown

Published: May 9, 2024

Quercetin, a natural compound, shows promising potential in wound healing by reducing fibrosis, limiting scar formation, and boosting fibroblast proliferation. However, its effectiveness is hindered poor solubility, resulting low bioavailability necessitating high doses for therapeutic efficacy. This study presents novel approach, fabricating quercetin-loaded microarray patches (MAPs) using widely employed solubility enhancement strategies. Fabricated MAPs exhibited favourable mechanical strength could be inserted into excised porcine skin to depth of 650 μm. Furthermore, formulations containing Soluplus

Language: Английский

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Smart Microneedles in Biomedical Engineering: Harnessing Stimuli‐Responsive Polymers for Novel Applications

Polymers for Advanced Technologies, Journal Year: 2024, Volume and Issue: 35(12)

Published: Dec. 1, 2024

ABSTRACT This review aims to provide a comprehensive analysis of recent advancements in smart microneedles (MNs) within the biomedical field, focusing on integration stimuli‐responsive polymers for enhanced therapeutic and diagnostic applications. Conventional drug delivery methods are known face limitations precision, safety, patient compliance, which can be addressed by innovative features MNs. Through use various polymers, these MNs have been designed react environmental or physiological cues, allowing on‐demand release, biomarker sensing, localized interventions. Fundamental materials used fabrication MNs, including metals, composite hydrogels, reviewed, different categories stimuli‐responsiveness, such as photo, electro, thermal, mechanical, biochemical, explored. Application‐specific designs areas delivery, cancer therapy, diabetes management, skin disease treatments also examined. this discussion, it is highlighted that poised play significant role advancing personalized noninvasive medical treatments.

Language: Английский

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In Silico Evaluation of Quercetin and Gallic Acid from Saraca Asoca as Potential Therapeutic Compounds for Wound Healing and Inflammation

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 8, 2025

This study discusses the possibility of quercetin and gallic acid, which are isolates Saraca Asoca, being applied to wound healing process. Three molecular docking simulations on following key targets with these compounds were conducted: vascular endothelial growth factor receptor 2 (VEGFR2) cyclooxygenase-2 (COX-2). Quercetin exhibited exceptional binding affinity at active site VEGFR2, a energy value -9.3 kcal/mol two hydrogen bonds Cys919 Glu917 besides more polar interactions catechol ring. Gallic acid was also strongly bound, − 6.0 kcal/mol, three Glu917. For COX-2, filled pocket 7.4 energy, establishing five such important residues as Lys83 Tyr115. A -6.5 produced by through significant Thr206, His207, Tyr385. These results explain promising pharmaceuticals in acceleration interaction VEGFR2 COX-2. is, therefore, crucial evidence bioactive substances Asoca raises possibilities that would be continued additional experiments for further review its mechanism action.

Language: Английский

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Collagen-based hydrogel derived from amniotic membrane loaded with quercetin accelerates wound healing by improving stereological parameters and reducing inflammation in a diabetic rat model

Tissue and Cell, Journal Year: 2025, Volume and Issue: 93, P. 102743 - 102743

Published: Jan. 16, 2025

Language: Английский

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Biochemical Strategy-Based Hybrid Hydrogel Dressing-Mediated In Situ Synthesis of Selenoproteins for DFU Immunity-Microbiota Homeostasis Regulation

Biomaterials, Journal Year: 2025, Volume and Issue: 317, P. 123114 - 123114

Published: Jan. 21, 2025

Language: Английский

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