Reproducibility of animal research in light of biological variation

Nature reviews. Neuroscience, Journal Year: 2020, Volume and Issue: 21(7), P. 384 - 393

Published: June 2, 2020

Language: Английский

---

The future of rodent models in depression research

Nature reviews. Neuroscience, Journal Year: 2019, Volume and Issue: 20(11), P. 686 - 701

Published: Oct. 2, 2019

Language: Английский

---

Behaviour Hallmarks in Alzheimer’s Disease 5xFAD Mouse Model

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(12), P. 6766 - 6766

Published: June 20, 2024

The 5xFAD transgenic mouse model widely used in Alzheimer’s disease (AD) research recapitulates many AD-related phenotypes with a relatively early onset and aggressive age-dependent progression. Besides developing amyloid peptide deposits alongside neuroinflammation by the age of 2 months, as well exhibiting neuronal decline 4 months that intensifies 9 these mice manifest broad spectrum behavioural impairments. In this review, we present extensive repertoire dysfunctions mice, organised into four categories: motor skills, sensory function, learning memory abilities, neuropsychiatric-like symptoms. problems, associated agility reflex movements, balance coordination, skeletal muscle typically arise time reach age. function (such taste, smell, hearing, vision) starts to deteriorate when buildups spread related anatomical structures. cognitive functions, encompassing such visual recognition, associative, spatial working, reference learning, show signs from 6 Concerning symptoms, comprising apathy, anxiety depression, willingness for exploratory behaviour, it is believed motivational changes emerge approximately Unfortunately, numerous studies different laboratories are often contradictory on conclusions drawn identification age, making preclinical rodent models not easily translatable humans. This variability likely due range factors animals themselves, housing husbandry conditions, experimental settings. forthcoming studies, greater clarity details conducting testing could minimise inconsistencies ensure reliability reproducibility results.

Language: Английский

---

Neuronal activity increases translocator protein (TSPO) levels

Molecular Psychiatry, Journal Year: 2020, Volume and Issue: 26(6), P. 2025 - 2037

Published: May 12, 2020

Abstract The mitochondrial protein, translocator protein (TSPO), is a widely used biomarker of neuroinflammation, but its non-selective cellular expression pattern implies roles beyond inflammatory processes. In the present study, we investigated whether neuronal activity modifies TSPO levels in adult central nervous system. First, single-cell RNA sequencing to generate landscape basal gene hippocampus (12 weeks old) C57BL6/N mice, followed by confocal laser scanning microscopy verify and non-neuronal cell populations. We then quantified mRNA after stimulating with distinct stimuli, including designer receptors exclusively activated drugs (DREADDs), exposure novel environment acute treatment psychostimulant drug, amphetamine. Single-cell demonstrated multi-cellular at conditions mouse hippocampus. Confocal confirmed that (astrocytes, microglia, vascular endothelial cells) cells cortical (medial prefrontal cortex) subcortical (hippocampus) brain regions. Stimulating through chemogenetic physiological (novel exposure) or psychopharmacological (amphetamine treatment) approaches led consistent increases neurons, not microglia astrocytes. Taken together, our findings show has potential modify These challenge general assumption altered binding unequivocally mirrors ongoing neuroinflammation emphasize need consider non-inflammatory interpretations some pathological contexts.

Language: Английский

---

Assessing the effects of stress on feeding behaviors in laboratory mice

eLife, Journal Year: 2022, Volume and Issue: 11

Published: Feb. 15, 2022

Stress often affects eating behaviors, increasing caloric intake in some individuals and decreasing it others. The determinants of feeding responses to stress are unknown, part because this issue is rarely studied rodents. We focused our efforts on the novelty-suppressed (NSF) assay, which uses latency eat as readout anxiety-like behavior, but assesses per se. explored how key variables experimental paradigms – estrous diurnal cyclicity, age duration social isolation, prandial state, diet palatability, elevated body weight influence stress-induced behavior food male female C57BL/6J mice. Latency novel environment increased both sexes across most conditions tested, while effects variable. In common NSF assay (i.e., lean mice light cycle), sex-specific length not main source variability. Under that more physiologically relevant for humans overweight active phase), now elicits robust hyperphagia . This model can be used identify underlying neuroendocrine neuronal substrates. Moreover, these studies serve a framework integrate cross-disciplinary anxiety related behaviors

Language: Английский

---

The Effects of Light and the Circadian System on Rhythmic Brain Function

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(5), P. 2778 - 2778

Published: March 3, 2022

Life on earth has evolved under the influence of regularly recurring changes in environment, such as 24 h light/dark cycle. Consequently, organisms have developed endogenous clocks, generating (circadian) rhythms that serve to anticipate these rhythmic changes. In addition circadian rhythms, which persist constant conditions and can be entrained environmental light drives behavior brain function, especially nocturnal laboratory rodents. recent decades, research made great advances elucidation molecular clockwork perception. This review summarizes role clock with a focus complex interaction between different components mammalian system. Furthermore, chronodisruption consequence at night, genetic manipulation, neurodegenerative diseases is briefly discussed.

Language: Английский

---

Assessing negative affect in mice during abstinence from alcohol drinking: Limitations and future challenges

Alcohol, Journal Year: 2022, Volume and Issue: 100, P. 41 - 56

Published: Feb. 15, 2022

Language: Английский

---

Influence of diurnal phase on behavioral tests of sensorimotor performance, anxiety, learning and memory in mice

Scientific Reports, Journal Year: 2022, Volume and Issue: 12(1)

Published: Jan. 10, 2022

Abstract Behavioral measurements in mice are critical tools used to evaluate the effects of interventions. Whilst nocturnal animals, many studies conduct behavioral tests during day. To better understand diurnal rhythm on mouse behaviors, we compared results from conducted active and inactive phases. C57BL/6 were this study; focus sensorimotor performance, anxiety, learning memory. Overall, our show exhibit slightly higher cutaneous sensitivity, long-term contextual memory, a greater avoidance escape response phase. We did not observe significant differences motor coordination, or spatial Furthermore, apart elevated-O-maze, there was no remarkable sex effect among these tests. This study provides information different phases types behavior demonstrates importance circadian cycle Although detect anxiety learning/memory, may interact with other factors influence behaviors.

Language: Английский

---

Effects of combined postweaning social isolation and ketamine administration on schizophrenia-like behaviour in male Sprague Dawley rats

Behavioural Brain Research, Journal Year: 2024, Volume and Issue: 476, P. 115214 - 115214

Published: Aug. 24, 2024

The pathophysiology behind negative and cognitive symptoms of schizophrenia is not well understood, thus limiting the effectiveness treatment on these symptoms. Developing reliable animal model vital to advance our understanding neurobiological basis disorder. Double hit used refer use two inducing interventions viz ketamine exposure social isolation. In this study we aim investigate robustness double in schizophrenia. On postnatal day (PND) 23, thirty-two male Sprague Dawley rats were randomly grouped into four equal groups as follows: group housed + saline (GH), (GHK), isolated (SI), (SIK). A single dose (16 mg/kg) was administered 3 times a week for weeks. Isolated animals singly throughout study. following behavioural tests carried out: elevated plus maze, three chamber interaction, resident intruder tests, novel object recognition (NOR). SIK exhibited high anxiety levels, with increased ACTH, corticosterone norepinephrine concentration when compared other groups. also presented reduced interaction decreased oxytocin concentration. more aggressive towards juvenile but had low testosterone or showed impaired visual learning memory expression proinflammatory cytokines. This suggest that robust than each alone.

Language: Английский

---

Light-Stress Influences the Composition of the Murine Gut Microbiome, Memory Function, and Plasma Metabolome

Frontiers in Molecular Biosciences, Journal Year: 2019, Volume and Issue: 6

Published: Oct. 18, 2019

The gut microbiome plays an important role in the mammalian host and when proper balance helps protect health prevent disease. Host environmental stress its influence on microbiome, health, disease is emerging area of research. Exposures to unnatural light cycles are becoming increasingly common due travel shift work. However, much remains unknown about how these changes health. This information needed understand predict relationship between response altered sleep cycles. In present study, we exposed three cohorts mice different cycle regimens for 12 consecutive weeks; including continuous light, dark, a standard dark regimen consisting h followed by dark. After exposure, motor memory behavior, composition fecal plasma metabolome were measured. Memory potential was significantly reduced whereas rotarod performance minimally affected. overall relatively constant over time. Bacteroidales Rikenellaceae more abundant while S24-7 light. after exposure differed from other conditions. Several metabolites, glycolic acid, tryptophan, pyruvate, several unidentified correlated Networking analyses showed that serotonin positively with microbial families ( Rikenellaceae, Ruminococcaceae , Turicibacteraceae ), tryptophan negatively abundance based exposure. study provides foundation future studies into mechanisms underlying murine during light-dark stress.

Language: Английский

---