Recent advances in neuropeptide signaling in Drosophila, from genes to physiology and behavior

Progress in Neurobiology, Journal Year: 2019, Volume and Issue: 179, P. 101607 - 101607

Published: March 22, 2019

Language: Английский

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A gene-specific T2A-GAL4 library for Drosophila

eLife, Journal Year: 2018, Volume and Issue: 7

Published: March 22, 2018

We generated a library of ~1000 Drosophila stocks in which we inserted construct the intron genes allowing expression GAL4 under control endogenous promoters while arresting transcription with polyadenylation signal 3' GAL4. This allows numerous applications. First, ~90% insertions essential cause severe loss-of-function phenotype, an effective way to mutagenize genes. Interestingly, 12/14 chromosomes engineered through CRISPR do not carry second-site lethal mutations. Second, 26/36 (70%) tested are rescued single UAS-cDNA construct. Third, phenotypes associated many can be reverted by excision UAS-flippase. Fourth, driven UAS-GFP/RFP reports tissue and cell-type specificity gene high sensitivity. report hundreds previously reported. Finally, cassettes replaced GFP or any DNA. These comprise powerful resource for assessing function.

Language: Английский

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Neuropeptides as Regulators of Behavior in Insects

Annual Review of Entomology, Journal Year: 2016, Volume and Issue: 62(1), P. 35 - 52

Published: Nov. 4, 2016

Neuropeptides are by far the largest and most diverse group of signaling molecules in multicellular organisms. They ancient important regulating a multitude processes. Their small proteinaceous character allowed them to evolve radiate quickly into numerous different molecules. On average, hundreds distinct neuropeptides present animals, sometimes with unique classes that do not occur distantly related species. Acting as neurotransmitters, neuromodulators, hormones, or growth factors, they extremely involved controlling growth, development, ecdysis, digestion, diuresis, many more physiological also crucial myriad behavioral actions associated feeding, courtship, sleep, learning memory, stress, addiction, social interactions. In general, behavior ensures an organism can survive its environment is defined any action change organism's relationship surroundings. Even though mode insects has been vigorously studied, relatively little known about only few model have investigated. Here, we provide overview roles play insect behavior. We conclude multiple need work concert coordinate certain behaviors. Additionally, studied date than single function.

Language: Английский

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A Peptidergic Circuit Links the Circadian Clock to Locomotor Activity

Current Biology, Journal Year: 2017, Volume and Issue: 27(13), P. 1915 - 1927.e5

Published: June 29, 2017

Language: Английский

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Substrates for Neuronal Cotransmission With Neuropeptides and Small Molecule Neurotransmitters in Drosophila

Frontiers in Cellular Neuroscience, Journal Year: 2018, Volume and Issue: 12

Published: March 23, 2018

It has been known for more than 40 years that individual neurons can produce one neurotransmitter and neuropeptides often are colocalized with small molecule neurotransmitters (SMNs). Over the much progress made in understanding functional consequences of cotransmission nervous system mammals. There also some excellent invertebrate models have revealed roles coexpressed SMNs increasing complexity, flexibility dynamics neuronal signaling. However, fly Drosophila there surprisingly few studies on cotransmission, although is ample evidence colocalization neuroactive compounds CNS, based both traditional techniques novel single cell transcriptome analysis. With hope to trigger interest initiating studies, this review summarizes what about circuits where different colocalized. Coexistence substances recorded neuron types such as neuroendocrine cells, interneurons, sensory cells motor neurons. Some highlighted here well established analysis learning memory, circadian clock networks regulating rhythmic activity sleep, olfaction, nociception, feeding, metabolic homeostasis, diuretic functions, reproduction developmental processes. One emerging trait broad role short neuropeptide F presynaptic facilitation a number circuits. This discusses relevance coexisting peptides intestine. Based recent transcriptomics data, it likely systems discussed just fraction total set occurs Drosophila. Thus, systematic search further anatomically defined near future.

Language: Английский

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Modulation of Drosophila post-feeding physiology and behavior by the neuropeptide leucokinin

PLoS Genetics, Journal Year: 2018, Volume and Issue: 14(11), P. e1007767 - e1007767

Published: Nov. 20, 2018

Behavior and physiology are orchestrated by neuropeptides acting as central neuromodulators circulating hormones. An outstanding question is how these function to coordinate complex competing behaviors. In Drosophila, the neuropeptide leucokinin (LK) modulates diverse functions, but mechanisms underlying interactions remain poorly understood. As a first step towards understanding mechanisms, we delineated LK circuitry that governs various aspects of post-feeding behavior. We found impaired signaling in Lk receptor (Lkr) mutants affects coordinated processes, including regulation stress, water homeostasis, feeding, locomotor activity, metabolic rate. Next, sought define populations neurons contribute different this physiology. find calcium activity abdominal ganglia (ABLKs), not two sets brain neurons, increases specifically following consumption, suggesting ABLKs regulate homeostasis its associated To identify targets peptide, mapped distribution Lkr expression, mined single-cell transcriptome dataset for genes coexpressed with Lkr, identified synaptic partners neurons. expression insulin-producing cells (IPCs), gut, renal tubules chemosensory cells, correlates well regulatory roles detected mutants. Furthermore, flies targeted knockdown IPCs displayed altered insulin-like peptides (DILPs) transcripts increased starvation resistance. Thus, some effects appear occur via DILP action. Collectively, our data suggest three have targets, modulate establishment post-prandial regulating distinct physiological processes behaviors such diuresis, metabolism, organismal insulin signaling. These findings provide platform investigating feeding-related neuroendocrine vital behavior

Language: Английский

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Hormonal axes in Drosophila: regulation of hormone release and multiplicity of actions

Cell and Tissue Research, Journal Year: 2020, Volume and Issue: 382(2), P. 233 - 266

Published: Aug. 22, 2020

Hormones regulate development, as well many vital processes in the daily life of an animal. Many these hormones are peptides that act at a higher hierarchical level animal with roles organizers globally orchestrate metabolism, physiology and behavior. Peptide can on multiple peripheral targets simultaneously convey basal states, such metabolic status sleep-awake or arousal across central neuronal circuits. Thereby, they coordinate responses to changing internal external environments. The activity neurosecretory cells is controlled either by (1) cell autonomous sensors, (2) other neurons relay signals from sensors tissues (3) feedback target cells. Thus, hormonal signaling axis commonly comprises several components. In mammals vertebrates, axes known, hypothalamic-pituitary-gonad hypothalamic-pituitary-thyroid reproduction respectively. It has been proposed basic organization evolutionarily old cellular homologs hypothalamic-pituitary system be found for instance insects. To obtain appreciation similarities between insect vertebrate axes, we review systems Drosophila. Our outlines major peptidergic pathways known Drosophila presents set schemes orchestrating systems. detailed larval adult displays only very those arthropods vertebrates.

Language: Английский

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The role of diuretic hormones (DHs) and their receptors in Drosophila

BMB Reports, Journal Year: 2023, Volume and Issue: 56(4), P. 209 - 215

Published: April 3, 2023

Maintaining internal homeostasis and regulating innate behaviors are essential for animal survival. In various species, a highly conserved neuroendocrine system integrates sensory inputs regulates physiological responses to environmental changes. Diuretic hormones 44 31, which homologs of mammalian corticotropin-releasing factor (CRF) calcitonin gene-related peptide (CGRP), respectively, control body fluid secretion in Drosophila. These neuropeptides their receptors have multiple roles, including the regulation body-fluid secretion, sleep:wake cycle, nutrientsensing, CO2-dependent response. This review discusses behavioral roles DH44 DH31 signaling pathways, consisting cells that secrete or peptides receptor-expressing organs. Further research is needed understand regulatory mechanisms processes mediated by these systems. [BMB Reports 2023; 56(4): 209-215].

Language: Английский

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Synaptic connectome of the Drosophila circadian clock

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Dec. 5, 2024

Abstract The circadian clock and its output pathways play a pivotal role in optimizing daily processes. To obtain insights into how diverse rhythmic physiology behaviors are orchestrated, we have generated comprehensive connectivity map of an animal using the Drosophila FlyWire brain connectome. Intriguingly, identified additional dorsal neurons, thus showing that network contains ~240 instead 150 neurons. We revealed extensive contralateral synaptic within discovered novel indirect light input to also elucidated via which modulates descending neurons known regulate feeding reproductive behaviors. Interestingly, observed sparse monosynaptic between downstream higher-order centers neurosecretory cells behavior physiology. Therefore, integrated single-cell transcriptomics receptor mapping decipher putative paracrine peptidergic signaling by Our analyses neuropeptides expressed suggest significantly enriches interconnectivity network.

Language: Английский

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Transcriptome and Neuroendocrinome Responses to Environmental Stress in the Model and Pest Insect Spodoptera frugiperda

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(2), P. 691 - 691

Published: Jan. 15, 2025

The fall armyworm, Spodoptera frugiperda, is one of the most notorious pest insects, causing damage to more than 350 plant species, and feared worldwide as an invasive species since it exhibits high adaptivity against environmental stress. Here, we therefore investigated its transcriptome responses four different types stresses, namely cold, heat, no water food. We used brain samples our interest was in neuroendocrine responses, while previous studies whole bodies larvae or moths. In general, were complex encompassed a vast array neuropeptides (NPs) biogenic amines (BAs). NPs mainly involved ion homeostasis regulation (ITP ITPL) metabolic pathways (AKH, ILP), this accompanied by changes BA (DA, OA) biosynthesis. Cold no-water stress changed NP gene expression with same patterns but clearly separated from each other, divergent pattern shown after no-food conclusion, data provide foundation important model insect candidate BAs other marker genes response stress, also potential new targets manage insects.

Language: Английский

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