Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2021–2022 DOI Creative Commons
David J. Harvey

Mass Spectrometry Reviews, Journal Year: 2024, Volume and Issue: unknown

Published: June 24, 2024

Abstract The use of matrix‐assisted laser desorption/ionization (MALDI) mass spectrometry for the analysis carbohydrates and glycoconjugates is a well‐established technique this review 12 th update original article published in 1999 brings coverage literature to end 2022. As with previous review, also includes few papers that describe methods appropriate by MALDI, such as sample preparation, even though ionization method not MALDI. follows same format reviews. It divided into three sections: (1) general aspects theory MALDI process, matrices, derivatization, imaging, fragmentation, quantification computer software structural identification. (2) Applications various types oligo‐ polysaccharides, glycoproteins, glycolipids, glycosides biopharmaceuticals, (3) other areas medicine, industrial processes, natural products glycan synthesis where extensively used. Much material relating applications presented tabular form. still an ideal carbohydrate analysis, particularly its ability produce single ions from each analyte advancements range show little sign diminishing.

Language: Английский

Multi-Omics Pipeline and Omics-Integration Approach to Decipher Plant’s Abiotic Stress Tolerance Responses DOI Open Access
Rajib Roychowdhury,

Soumya Prakash Das,

Amber Gupta

et al.

Genes, Journal Year: 2023, Volume and Issue: 14(6), P. 1281 - 1281

Published: June 16, 2023

The present day's ongoing global warming and climate change adversely affect plants through imposing environmental (abiotic) stresses disease pressure. major abiotic factors such as drought, heat, cold, salinity, etc., hamper a plant's innate growth development, resulting in reduced yield quality, with the possibility of undesired traits. In 21st century, advent high-throughput sequencing tools, state-of-the-art biotechnological techniques bioinformatic analyzing pipelines led to easy characterization plant traits for stress response tolerance mechanisms by applying 'omics' toolbox. Panomics pipeline including genomics, transcriptomics, proteomics, metabolomics, epigenomics, proteogenomics, interactomics, ionomics, phenomics, have become very handy nowadays. This is important produce climate-smart future crops proper understanding molecular responses genes, transcripts, proteins, epigenome, cellular metabolic circuits resultant phenotype. Instead mono-omics, two or more (hence 'multi-omics') integrated-omics approaches can decipher well. Multi-omics-characterized be used potent genetic resources incorporate into breeding program. For practical utility crop improvement, multi-omics particular combined genome-assisted (GAB) being pyramided improved yield, food quality associated agronomic open new era omics-assisted breeding. Thus, together are able processes, biomarkers, targets engineering, regulatory networks precision agriculture solutions crop's variable ensure security under changing circumstances.

Language: Английский

Citations

88
Quantitative mass spectrometry imaging: therapeutics & biomolecules DOI Creative Commons
Joseph Holbrook,

Gabrielle E. Kemper,

Amanda B. Hummon

et al.

Chemical Communications, Journal Year: 2024, Volume and Issue: 60(16), P. 2137 - 2151

Published: Jan. 1, 2024

In this feature article, we discuss quantitative mass spectrometry imaging of therapeutics and biomolecules conducted by recent studies for matrix-assisted laser desorption/ionization (MALDI) desorption electrospray ionization (DESI) techniques.

Language: Английский

Citations

15
Plant and algal lipidomes: Analysis, composition, and their societal significance DOI Creative Commons
Juliette Jouhet, Eliana Alves, Yohann Boutté

et al.

Progress in Lipid Research, Journal Year: 2024, Volume and Issue: 96, P. 101290 - 101290

Published: July 31, 2024

niveau recherche, publiés ou non, émanant des établissements d'enseignement et de recherche français étrangers, laboratoires publics privés.

Citations

9
Understanding the physiological mechanisms and therapeutic targets of diseases: Lipidomics strategies DOI
Qiang Yang, Ying Cai,

Zhibo Wang

et al.

Life Sciences, Journal Year: 2025, Volume and Issue: 363, P. 123411 - 123411

Published: Jan. 21, 2025

Language: Английский

Citations

1
Recent development of microfluidic biosensors for the analysis of antibiotic residues DOI
Xiaorui Wang,

Yaoshuang Xie,

Ling Lin

et al.

TrAC Trends in Analytical Chemistry, Journal Year: 2022, Volume and Issue: 157, P. 116797 - 116797

Published: Oct. 13, 2022

Language: Английский

Citations

30
A Five-Year Update on Matrix Compounds for MALDI-MS Analysis of Lipids DOI Creative Commons
Jenny Leopold, Patricia Prabutzki,

Kathrin M. Engel

et al.

Biomolecules, Journal Year: 2023, Volume and Issue: 13(3), P. 546 - 546

Published: March 16, 2023

Matrix-assisted laser desorption and ionization (MALDI) is a widely used soft-ionization technique of modern mass spectrometry (MS). MALDI enables the analysis nearly all chemical compounds—including polar apolar (phospho)lipids—with minimum extent fragmentation. has some particular advantages (such as possibility to acquire spatially-resolved spectra) competitive with simultaneously developed ESI (electrospray ionization) MS. Although there are still methodological aspects that need be elucidated in more detail, it obvious careful selection an appropriate matrix plays most important role (lipid) analysis. Some lipid classes can detected exclusively if optimum used, determines sensitivity by which within mixture. Since is, thus, crucial for results, we provide here update on progress field since our original review this journal 2018. Thus, only development during last five years considered, lipids sorted according increasing complexity, starting free fatty acids ending cardiolipins phosphoinositides.

Language: Английский

Citations

22
Seipin deficiency-induced lipid dysregulation leads to hypomyelination-associated cognitive deficits via compromising oligodendrocyte precursor cell differentiation DOI Creative Commons
Wenli Cui, Jing Yang,

Chuanyun Tu

et al.

Cell Death and Disease, Journal Year: 2024, Volume and Issue: 15(5)

Published: May 21, 2024

Abstract Seipin is one key mediator of lipid metabolism that highly expressed in adipose tissues as well the brain. Lack gene, Bscl2 , leads to not only severe metabolic disorders but also cognitive impairments and motor disabilities. Myelin, composed mainly lipids, facilitates nerve transmission important for coordination learning. Whether deficiency-leaded defects learning underlined by dysregulation its consequent myelin abnormalities remains be elucidated. In present study, we verified expression oligodendrocytes (OLs) their precursors, oligodendrocyte precursor cells (OPCs), demonstrated deficiency compromised OPC differentiation, which led decreased OL numbers, protein, myelinated fiber proportion thickness myelin. Deficiency resulted impaired spatial cognition mice. Mechanistically, suppressed sphingolipid metabolism-related genes OPCs caused morphological droplets (LDs), markedly impeded differentiation. Importantly, rosiglitazone, agonist PPAR-gamma, substantially restored phenotypes resulting from deficiency, such aberrant LDs, reduced sphingolipids, obstructed neurobehavioral defects. Collectively, study elucidated how deficiency-induced deficits via impairing myelination, may pave way developing novel intervention strategy treating metabolism-involved neurological disorders.

Language: Английский

Citations

6
Mass Spectrometry Imaging Reveals the Morphology-Dependent Toxicological Effects of Nanosilvers on Multiple Organs of Adult Zebrafish (Danio rerio) DOI

Qingrong Xie,

Zhen Li, Yingying Chen

et al.

Environmental Science & Technology, Journal Year: 2024, Volume and Issue: 58(23), P. 10015 - 10027

Published: May 27, 2024

Nanosilvers with multifarious morphologies have been extensively used in many fields, but their morphology-dependent toxicity toward nontarget aquatic organisms remains largely unclear. Herein, we matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) to investigate the toxicological effects of silver nanomaterials various on spatially resolved lipid profiles within multiple organs adult zebrafish, especially for gill, liver, and intestine. Integrated histopathology, enzyme activity, accumulated Ag contents amounts, as well MSI results, found that nanosilvers exhibit nanotoxicity by disrupting levels producing oxidative stress. Silver nanospheres (AgNSs) had highest whereas nanoflakes (AgNFs) exhibited greater than nanowires (AgNWs). Levels differential phospholipids, such PC, PE, PI, PS, were associated nanosilver morphology. Notably, AgNSs induced organs, brain, while AgNWs AgNFs caused intestine AgNSs. Lipid functional disturbance stress further inflammation membrane damage after exposure nanosilvers, respect sphere Taken together, these findings will contribute clarifying mechanisms different zebrafish.

Language: Английский

Citations

5
Planar chromatography – Current practice and future prospects DOI Creative Commons
Ian D. Wilson, Colin F. Poole

Journal of Chromatography B, Journal Year: 2022, Volume and Issue: 1214, P. 123553 - 123553

Published: Nov. 28, 2022

Planar chromatography, in the form of thin-layer or high-performance chromatography (TLC, HPTLC), continues to provide a robust and widely used separation technique. It is unrivaled as simple rapid qualitative method for mixture analysis, finding bioactive components mixtures. The format TLC/HPTLC also provides unique preserving separation, enabling further investigation interest (including quantification/structure determination) separated both time space from original analysis. current practice planar areas development technology are reviewed promising future directions use outlined.

Language: Английский

Citations

21
Liver Metabolic Dysregulation Induced by Polypropylene Nano- and Microplastics in Nile Tilapia Using Internal Extractive Electrospray Ionization Mass Spectrometry DOI
Xiaokang Wu, Ming Li,

Shengao Yang

et al.

Analytical Chemistry, Journal Year: 2023, Volume and Issue: 95(20), P. 7863 - 7871

Published: May 9, 2023

Understanding the metabolic disorders induced by nano- and microplastics in aquatic organisms at molecular level could help us understand potential toxicity of more thoroughly provide a fundamental scientific basis for regulating usage management plastic products. In this research, effect polypropylene nanoplastics (PP-NPs) (PP-MPs) on metabolites tilapia liver was comprehensively investigated internal extractive electrospray ionization mass spectrometry (iEESI-MS). A partial least-squares discriminant analysis (PLS-DA) one-component variance (ANOVA) were used selecting 46 differential metabolites, including phospholipids, amino acids, peptides, carbohydrates, alkaloids, purines, pyrimidines, nucleosides. Pathway enrichment showed significant effects glycerophospholipid metabolism, arginine proline aminoacyl-tRNA biosynthesis after exposed to PP-N/MPs. Dysregulation these is mainly reflected possible induction hepatitis, oxidative stress, other symptoms. The application iEESI-MS technology without sample pretreatment study under interference provides promising analytical method environmental toxicology research.

Language: Английский

Citations

12