IP Archives of Cytology and Histopathology Research,
Journal Year:
2023,
Volume and Issue:
8(2), P. 83 - 89
Published: July 15, 2023
Chronic
wounds
are
that
fail
to
progress
through
the
normal
stages
of
healing
and
remain
open
for
an
extended
period
time.
These
often
require
specialized
treatment
approaches.
While
genes
involved
in
wound
play
important
roles
chronic
as
well,
specific
genetic
factors
contributing
still
being
studied.
Some
have
been
implicated
their
potential
include
Transforming
Growth
Factor-Beta
(TGF-β)
Family,
Matrix
Metalloproteinases
(MMPs),
Factor-Alpha
(TGF-α),
Hypoxia-Inducible
Factor-1
(HIF-1),
Vascular
Endothelial
Factor
(VEGF),
Nitric
Oxide
Synthase
(NOS)
Wound-Healing-Associated
microRNAs.
It's
note
multifactorial,
requires
a
comprehensive
approach
addressing
various
beyond
alone.
Other
aspects
such
underlying
medical
conditions,
infection,
local
environment,
patient-specific
also
crucial
management.
International Journal of Molecular Sciences,
Journal Year:
2024,
Volume and Issue:
25(13), P. 6821 - 6821
Published: June 21, 2024
Aging
is
associated
with
a
decline
in
the
functionality
of
various
cell
types,
including
dermal
fibroblasts,
which
play
crucial
role
maintaining
skin
homeostasis
and
wound
healing.
Chronic
inflammation
increased
reactive
oxygen
species
(ROS)
production
are
hallmark
features
aging,
contributing
to
impaired
MicroRNA-146a
(miR-146a)
has
been
implicated
as
critical
regulator
oxidative
stress
different
yet
its
aged
fibroblasts
potential
relevance
healing
remains
poorly
understood.
We
hypothesize
that
miR-146a
differentially
expressed
overexpression
will
decrease
aging-induced
inflammatory
responses
ROS
production.
Primary
were
isolated
from
17-week-old
(young)
88-week-old
(aged)
mice.
Overexpression
was
achieved
through
mimic
transfection.
detected
using
reliable
fluorogenic
marker,
2,7-dichlorofluorescin
diacetate.
Real-time
PCR
used
quantify
relative
gene
expression.
Our
investigation
revealed
significant
reduction
expression
compared
their
younger
counterparts.
Moreover,
exhibited
heightened
levels
Importantly,
transfection
led
substantial
modulation
NF-kB
pathway
fibroblasts.
Additionally,
production,
downregulation
NOX4
These
findings
underscore
pivotal
mitigating
both
highlighting
therapeutic
target
for
addressing
age-related
Antioxidants,
Journal Year:
2025,
Volume and Issue:
14(4), P. 486 - 486
Published: April 18, 2025
Transforming
Growth
Factor-Beta
1
(TGF-β1)
plays
a
pivotal
role
in
the
differentiation
of
fibroblasts
into
myofibroblasts,
which
is
critical
process
tissue
repair,
fibrosis,
and
wound
healing.
Upon
exposure
to
TGF-β1,
acquire
contractile
phenotype
secrete
collagen
extracellular
matrix
components.
Numerous
studies
have
identified
hydrogen
peroxide
(H2O2)
as
key
downstream
effector
TGF-β1
this
pathway.
H2O2
functions
signalling
molecule,
regulating
various
cellular
processes
mostly
through
post-translational
redox
modifications
cysteine
thiol
groups
specific
proteins.
In
study,
we
used
primary
human
skin
fibroblast
cultures
investigate
oxidative
mechanisms
triggered
by
TGF-β1.
We
analyzed
expression
redox-related
genes,
evaluated
effects
genetic
pharmacological
inhibition
H2O2-producing
enzymes,
employed
an
unbiased
proteomics
approach
(OxICAT)
identify
proteins
undergoing
reversible
oxidation.
Our
findings
revealed
that
treatment
upregulated
oxidant-generating
genes
while
downregulating
antioxidant
genes.
Low
concentrations
diphenyleneiodonium
mitigated
myofibroblast
mitochondrial
oxygen
consumption,
suggesting
involvement
flavoenzyme
process.
Furthermore,
increased
oxidation
highly
conserved
residues
such
epidermal
growth
factor
receptor,
filamin
A,
fibulin-2,
endosialin
during
Collectively,
study
provides
insights
sources
highlights
novel
underpinning
fibroblast-to-myofibroblast
differentiation.
Chinese Medicine,
Journal Year:
2024,
Volume and Issue:
19(1)
Published: June 19, 2024
Abstract
Background
Extended
contact
with
silica
particles
can
lead
to
Silicosis,
a
chronic
lung
condition
lacking
established
treatment
protocols
or
clear
mechanisms
of
development.
The
urgency
for
innovative
treatments
arises
from
the
unavailability
effective
methodologies.
origin
silica-induced
pulmonary
fibrosis
includes
essential
processes
such
as
macrophage
activation
and
conversion
fibroblasts
into
myofibroblasts,
oxidative
stress
playing
pivotal
role.
Shionone
(SHI),
triterpenoid
extracted
Aster
tataricus
plant,
is
recognized
its
extensive
health
benefits.
This
study
explores
capability
SHI
alleviate
effects
in
mice.
Methods
investigation
explored
impact
on
inflammation
at
different
stages
(early
late)
triggered
by
mice,
focusing
specifically
initial
more
developed
phases.
It
comprised
an
analysis
isolated
peritoneal
macrophages
mice
elucidate
SHI's
therapeutic
potential
underlying
mechanism.
methodology
employed
encompassed
quantitative
PCR,
immunofluorescence,
flow
cytometry,
western
blotting
examine
activity
their
transition
myofibroblasts.
nuclear
factor
erythroid
2-related
2
(Nrf2)
pathway
was
confirmed
via
immunofluorescence
blot
studies.
antioxidative
properties
were
evidenced
measurement
reactive
oxygen
species
(ROS)
mitochondrial
ROS
within
both
fibroblasts,
using
2′,
7′-dichlorodihydrofluorescein
diacetate
MitoSOX,
respectively.
relevance
further
underscored
applying
ML385
Nrf2
siRNA
gauge
effectiveness.
Results
Starting
early
countered
harmful
caused
silica,
while
initiating
later
phase
decelerated
advancement
fibrosis.
action
linked
signaling
pathway,
boost
antioxidant
enzyme
levels,
decrease
affected
silica.
Furthermore,
prevented
myofibroblasts
prompted
TGF-β,
along
resultant
stress.
beneficial
outcomes
negated
when
applied,
highlighting
role
efficacy.
Conclusion
plays
significant
stimulating
thereby
defending
against
inflammatory
reactions
macrophages,
inhibiting
due
TGF-β.
suggests
that
viable
option
treating
suffering
silicosis.
Antioxidants,
Journal Year:
2023,
Volume and Issue:
12(6), P. 1228 - 1228
Published: June 7, 2023
Human
corneal-endothelial
cells
(hCEnCs)
are
located
on
the
inner
layer
of
cornea.
Injury
to
CEnCs
leads
permanent
corneal
edema,
requiring
transplantation.
NADPH
oxidase
4
(NOX4)
has
been
reported
be
implicated
in
pathogenesis
diseases.
Thus,
we
investigated
role
NOX4
this
study.
In
an
animal
study,
siRNA
for
(siNOX4)
or
plasmid
(pNOX4)
was
introduced
into
endothelium
rats
by
electroporation,
using
a
square-wave
electroporator
(ECM830,
Havard
apparatus)
decrease
increase
expression
NOX4,
respectively,
and
rat
corneas
were
cryoinjured
through
contact
with
metal
rod
3
mm
diameter
frozen
liquid
nitrogen
10
min.
The
immunofluorescence
staining
8-OHdG
showed
that
levels
decreased
siNOX4
group
compared
siControl,
increased
pNOX4
pControl
at
one
week
after
treatment.
Without
cryoinjury,
opacity
more
severe,
density
lower,
pNOX4-treated
pControl.
After
transparent,
CEnC
higher,
siNOX4-treated
rats.
hCEnCs
cultured
transfected
pNOX4.
silencing
resulted
normal
cell
shape,
higher
viability,
proliferation
rate
than
those
while
overexpression
had
opposite
effect.
number
senescent
intracellular
oxidative
stress
levels.
ATF4
ATF6
levels,
nuclear
translocation
XBP-1,
which
is
endoplasmic
reticulum
(ER)
marker,
Additionally,
mitochondrial
membrane
potential
hyperpolarized
depolarized
overexpression.
LC3II
marker
autophagy,
conclusion,
plays
pivotal
wound-healing
senescence
hCEnCs,
modulating
stress,
ER
autophagy.
regulation
may
therapeutic
strategy
regulating
homeostasis
CEnCs,
treating
Journal of Leukocyte Biology,
Journal Year:
2023,
Volume and Issue:
115(5), P. 882 - 892
Published: Sept. 29, 2023
Abstract
Adipose-derived
stem
cells
(ADSCs)
have
been
proven
to
promote
healing
in
diabetic
wounds,
which
are
one
of
the
most
serious
chronic
refractory
wounds.
However,
reactive
oxygen
species
(ROS)
induced
by
high
glucose
(HG)
lead
oxidative
stress
and
aging
ADSCs,
limits
therapeutic
effect
ADSCs.
In
this
study,
we
investigated
role
GKT137831,
a
NOX1/4
inhibitor
that
can
reduce
ROS
production,
protecting
ADSCs
from
hyperglycemia
wound
healing.
vitro,
levels
NOX4
expression
were
increased
after
HG
treatment
while
marker
malondialdehyde
was
increased;
mitochondrial
membrane
potential
decreased;
inflammatory
aging–related
indicators
such
as
p16,
p21,
matrix
metalloproteinase-1
(MMP1),
MMP3,
interleukin-6,
β-galactosidase
migration
weakened.
vivo,
constructed
mouse
model
found
combination
GKT137831
synergistically
promoted
21-day
rate,
collagen
hydroxyproline,
number
blood
vessels
CD31,
reduced
MMP1,
p21.
These
results
suggest
could
protect
enhance
on
Food & Function,
Journal Year:
2024,
Volume and Issue:
15(15), P. 7849 - 7864
Published: Jan. 1, 2024
As
the
first
line
of
defense,
skin
is
equipped
with
various
physiological
mechanisms
positioned
to
prevent
incoming
oxidative
damage
from
numerous
environmental
insults.
