Muscle of obese insulin-resistant humans exhibits losses in proteostasis and attenuated proteome dynamics that are improved by exercise training DOI Creative Commons
Kanchana Srisawat, Connor A. Stead, Katie Hesketh

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: March 20, 2023

Abstract We examined muscle proteostasis in obese insulin-resistant (OIR) individuals to determine whether endurance exercise could positively influence proteome dynamics this population. Male OIR (n = 3) and lean, healthy controls (LHC; n 4) were recruited underwent a 14-d measurement protocol of daily deuterium oxide (D 2 O) consumption serial biopsies vastus lateralis muscle. The group then completed 10-weeks high-intensity interval training (HIIT), encompassing 3 sessions per week cycle ergometer with 1 min intervals at 100 % maximum aerobic power (W max ) interspersed by recovery periods. number session progressed from 4 8, during weeks 8-10 the was repeated. abundance turnover rates 880 301 proteins, respectively, measured. LHC exhibited 352 differences ( p < 0.05, false discovery rate 0.05) protein turnover. enriched markers metabolic stress, misfolding components ubiquitin-proteasome system, many these proteins less compared HIIT altered 53 increased 22 tended restore proteostasis, evidenced increasing normalizing proteasome composition participants. In conclusion, obesity insulin resistance are associated compromised which can be partially restored exercise.

Language: Английский

Mitochondrial complex I ROS production and redox signaling in hypoxia DOI Creative Commons
Chidozie Nwabuisi Okoye, Shon A. Koren, Andrew P. Wojtovich

et al.

Redox Biology, Journal Year: 2023, Volume and Issue: 67, P. 102926 - 102926

Published: Oct. 16, 2023

Mitochondria are a main source of cellular energy. Oxidative phosphorylation (OXPHOS) is the major process aerobic respiration. Enzyme complexes electron transport chain (ETC) pump protons to generate protonmotive force (Δp) that drives OXPHOS. Complex I an entry point into ETC. oxidizes nicotinamide adenine dinucleotide (NADH) and transfers electrons ubiquinone in reaction coupled with proton pumping. also produces reactive oxygen species (ROS) under various conditions. The enzymatic activities complex can be regulated by metabolic conditions serves as regulatory node ROS plays diverse roles cell metabolism ranging from physiologic pathologic Progress our understanding indicates release important signaling functions. Increasing evidence suggests mismatch energy production demand. In this article, we review role sensing acute hypoxia.

Language: Английский

Citations

105
Hormesis determines lifespan DOI Creative Commons
Edward J. Calabrese,

Marc Nascarella,

Peter Pressman

et al.

Ageing Research Reviews, Journal Year: 2024, Volume and Issue: 94, P. 102181 - 102181

Published: Jan. 4, 2024

Language: Английский

Citations

36
The Impact of Physical Exercise on Oxidative and Nitrosative Stress: Balancing the Benefits and Risks DOI Creative Commons
Qing Meng, Chun-Hsien Su

Antioxidants, Journal Year: 2024, Volume and Issue: 13(5), P. 573 - 573

Published: May 7, 2024

This review comprehensively evaluates the effects of physical exercise on oxidative and nitrosative stress, mainly focusing role antioxidants. Using a narrative synthesis approach, data from empirical studies, reviews, systematic meta-analyses published between 2004 2024 were collated databases like PubMed, EBSCO (EDS), Google Scholar, culminating in inclusion 41 studies. The quality these studies was rigorously assessed to ensure clarity objectives, coherence arguments, comprehensive literature coverage, depth critical analysis. Findings revealed that moderate enhances antioxidant defenses through hormesis, while excessive may exacerbate stress. also highlights natural dietary antioxidants are beneficial, high-dose supplements could impede positive adaptations exercise. In conclusion, calls for more focused research tailored nutrition plans further understand complex interactions optimize health outcomes athletes general population.

Language: Английский

Citations

17
The Impact of Physical Exercise on Oxidative and Nitrosative Stress: Balancing the Benefits and Risks DOI Open Access
Qing Meng, Chun-Hsien Su

Published: April 11, 2024

This general review assesses the impact of physical exercise on oxidative and nitrosative stress counteractive role antioxidants, drawing from a systematic literature search up to March 2024 in databases like PubMed Web Science. The highlights studies effect production reactive oxygen species (ROS) nitrogen (RNS) antioxidant responses both human animal models based rigorous inclusion exclusion criteria. Due variety types participants&#039; characteristics, narrative synthesis approach was adopted. findings indicate that moderate boosts defenses, known as hormesis, whereas excessive activity may increase damage. also explores complex effects supplementation, revealing while natural dietary antioxidants support health, high-dose supplements could potentially hinder positive adaptations exercise. Recommendations for athletes active population highlight importance balancing intake optimize health benefits. concludes with call more detailed research tailored nutrition plans dissect these intricate relationships further.

Language: Английский

Citations

16
Redox regulation of UPR signalling and mitochondrial ER contact sites DOI Creative Commons

Jose C. Casas-Martinez,

Afshin Samali, Brian McDonagh

et al.

Cellular and Molecular Life Sciences, Journal Year: 2024, Volume and Issue: 81(1)

Published: June 7, 2024

Mitochondria and the endoplasmic reticulum (ER) have a synergistic relationship are key regulatory hubs in maintaining cell homeostasis. Communication between these organelles is mediated by mitochondria ER contact sites (MERCS), allowing exchange of material information, modulating calcium homeostasis, redox signalling, lipid transfer regulation mitochondrial dynamics. MERCS dynamic structures that allow cells to respond changes intracellular environment under normal homeostatic conditions, while their assembly/disassembly affected pathophysiological conditions such as ageing disease. Disruption protein folding lumen can activate Unfolded Protein Response (UPR), promoting remodelling membranes formation. The UPR stress receptor kinases PERK IRE1, located at or close MERCS. signalling be adaptive maladaptive, depending on whether disruption transient sustained. Adaptive via increase import, metabolism dynamics, maladaptive result excessive import activation apoptotic pathways. Targeting assembly an attractive therapeutic approach for range age-related neurodegeneration sarcopenia. This review highlights emerging evidence related role orchestrating inter-organelle communication mitochondria, ultimately determination function fate.

Language: Английский

Citations

11
Chaihuang Yishen Granule ameliorates mitochondrial homeostasis by upregulating PRDX5/TFAM axis to inhibit renal fibrosis in CKD DOI

Linghui Xu,

Ruizhi Tan,

Jing‐Yi Lin

et al.

Phytomedicine, Journal Year: 2025, Volume and Issue: unknown, P. 156426 - 156426

Published: Jan. 1, 2025

Language: Английский

Citations

2
Reactive Oxygen Species and H. pylori Infection: A Comprehensive Review of Their Roles in Gastric Cancer Development DOI Creative Commons
Dhiraj Kumar Sah, Archana Arjunan, Bora Lee

et al.

Antioxidants, Journal Year: 2023, Volume and Issue: 12(9), P. 1712 - 1712

Published: Sept. 2, 2023

Gastric cancer (GC) is the fifth most common worldwide and makes up a significant component of global burden. Helicobacter pylori (H. pylori) influential risk factor for GC, with International Agency Research on Cancer classifying it as Class I carcinogen GC. H. has been shown to persist in stomach acid decades, causing damage stomach’s mucosal lining, altering gastric hormone release patterns, potentially function. Epidemiological studies have that eliminating reduces metachronous cancer. Evidence shows various molecular alterations are present precancerous lesions associated an infection. However, although can cause oxidative stress-induced cancer, antioxidants being treatment exact mechanism underlying GC etiology not fully understood. This review provides overview recent research exploring pathophysiology pylori-induced stress antioxidant supplements reduce or even eliminate occurrence.

Language: Английский

Citations

19
Peroxiredoxin 2 regulates DAF-16/FOXO mediated mitochondrial remodelling in response to exercise that is disrupted in ageing DOI Creative Commons
Xia Qin, Penglin Li,

José C. Casas-Martinez

et al.

Molecular Metabolism, Journal Year: 2024, Volume and Issue: 88, P. 102003 - 102003

Published: Aug. 6, 2024

Ageing is associated with mitochondrial dysfunction and increased oxidative stress. Exercise generates endogenous reactive oxygen species (ROS) promotes rapid remodelling. We investigated the role of Peroxiredoxin 2 (PRDX-2) in adaptations to exercise ageing using Caenorhabditis elegans as a model system. PRDX-2 was required for remodelling response mediated by DAF-16 transcription factor activation regulation fusion gene eat-3. Employing an acute recovery cycle, we demonstrated exercise-induced ER contact sites (MERCS) assembly dependent on signalling. There fragmentation, elevated ROS altered redox state concomitant impaired nuclear localisation during ageing. Similarly, prdx-2 mutant strain exhibited fragmentation failure activate fusion. Collectively, our data highlight critical orchestrating physiological stress regulating localisation.

Language: Английский

Citations

5
Promising Tools into Oxidative Stress: A Review of Non-Rodent Model Organisms DOI Creative Commons
Yuhao Zhang, Yun Li, Tianyi Ren

et al.

Redox Biology, Journal Year: 2024, Volume and Issue: 77, P. 103402 - 103402

Published: Oct. 17, 2024

Language: Английский

Citations

4
Rosella flower extract supplementation after physical exercise proven to increase superoxide dismutase levels as one of the enzymatic antioxidants to reduce oxidative stress DOI
Novadri Ayubi, Afif Rusdiawan, Setiyo Hartoto

et al.

Published: March 19, 2025

This study aims to analyze rosella flower extract supplementation on superoxide dismutase (SOD) levels after physical activity. experimental research uses a pre- and post-control group design. Subjects were selected using purposive sampling technique. A total of 20 healthy men aged 19–25 years participated in this divided into two groups, namely K1 with placebo K2 at dose 500 mg. Data collection began gathering data subject characteristics. Next, the subjects asked warm up perform activity form running 2400 meters moderate intensity. One hour meters, blood was drawn for pre-test measure SOD levels. Then, interventions administered according respective groups: mg K2. Sixty minutes intervention, post-test draw conducted. The study’s findings demonstrated that administering exercise significantly increased (p < 0.05*). Furthermore, there also notable rise control group’s It can be concluded physiological increase is result moderately intense However, recommended as an additional supplement further boost body’s antioxidant maintain health.

Language: Английский

Citations

0