Efficient synthesis of squalene by cytoplasmic-peroxisomal engineering and regulating lipid metabolism in Yarrowia lipolytica

Bioresource Technology, Journal Year: 2024, Volume and Issue: unknown, P. 130379 - 130379

Published: Jan. 1, 2024

Language: Английский

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Response surface methodology and repeated-batch fermentation strategies for enhancing lipid production from marine oleaginous Candida parapsilosis Y19 using orange peel waste

Microbial Cell Factories, Journal Year: 2025, Volume and Issue: 24(1)

Published: Jan. 10, 2025

Abstract Oleaginous yeasts are considered promising sources for lipid production due to their ability accumulate high levels of lipids under appropriate growth conditions. The current study aimed isolate and identify oleaginous having superior quantities lipids; enhancing using response surface methodology repeated-batch fermentation. Results revealed that, twenty marine were isolated, the most potent producer was Candida parapsilosis Y19 according qualitative screening test Nile-red dye. Orange peels used as substrate where C. produced 1.14 g/l at 23.0% in batch To enhance production, statistical optimization Taguchi design through Response carried out. Total increased 2.46 content 30.7% optimal conditions of: orange peel 75 g/l, peptone 7 yeast extract 5 inoculum size 2% ( v/v ), pH incubation period 6 d. Furthermore, fermentation enhanced total 4.19 folds (4.78 g/l) compared culture (before optimization). Also, 1.7 (39.1%) Fatty acid profile includes unsaturated fatty acids (USFAs) 74.8% saturated (SFAs) 25.1%. Additionally, fermentation, major oleic 45.0%; followed by linoleic 26.0%. In conclusion, is a strain production. both optimizations RSM efficient methods from Y19.

Language: Английский

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Recent advances in the life cycle assessment of biodiesel production linked to azo dye degradation using yeast symbionts of termite guts: A critical review

Energy Reports, Journal Year: 2022, Volume and Issue: 8, P. 7557 - 7581

Published: June 13, 2022

Beyond energy crisis, biodiesel offers renewable and unlimited option to the challenges associated with fossil fuels depletion. Biodiesel could reduce carbon foot print gas emissions. Therefore, China has set an audacious goal of neutrality by 2060. In order cost production, it is critical look for alternative feedstocks unconventional resources. can be produced from sustainable like edible non-edible oils, insects, municipal sewage sludge, oleaginous microorganisms. Existing life cycle assessments yeast-based production are required assess potential environmental impacts obtain a holistic picture. The circular economy paradigm recently emerged as viable linear, unsustainable consumption systems. Given legislative requirements principle, use textile azo dye wastewater promising its management. annual disposal 4,500,000 tons dyes socioeconomic concern due their carcinogenic potential. Azo account over 70% global industrial demand. Biological-mediated degradation thought cost-effective, environmentally friendly when compared physical chemical approaches. To this end, yeasts play significant role, owing high growth rate, tolerance extreme conditions, ability effectively degrade lignin aromatic compounds, concurrently representing highly feedstock production. Interestingly, emphasis been given on novel symbiotic isolated termite guts microbiomes multiple biotechnological applications. review focuses recent findings degrading inhabiting guts. While capturing research advancements emphasizes concept using dyes/aromatic wastes

Language: Английский

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New trends in microbial lipid-based biorefinery for fermentative bioenergy production from lignocellulosic biomass

Biofuel Research Journal, Journal Year: 2024, Volume and Issue: 11(01), P. 2040 - 2064

Published: March 1, 2024

Using oleaginous microbial lipid-based biorefinery from lignocellulosic biomass (LCB) to produce fermentative bioenergy (i.e., biodiesel) represents an innovative second-generation fuel production technology. These lipids are predominantly intracellular triglycerides that accumulate through the metabolism of sugars in fermentation following pretreatment and enzymatic hydrolysis LCB. This review investigates recent advances lipid LCB, focusing on factors influencing lead producers, different methods physical, chemical, biological, combined pretreatment), approaches, novel bioprocessing strategies microbes-specific model specific), engineering techniques microbes genetic metabolic alterations). The study demonstrates yeasts can synthesize significantly higher quantities when incorporated into system, known as separated production, various methods. Interestingly, CRISPR is found be most suitable way genetically metabolically for increased synthesis. also explores economically viable addressing associated challenges, outlines future directions, including comprehensive techno-economic life cycle assessments. offers invaluable insights highlighting potential significant technological environmental enhancements ongoing research development efforts.

Language: Английский

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A review of lipid accumulation by oleaginous yeasts: Culture mode

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 919, P. 170385 - 170385

Published: Feb. 2, 2024

Language: Английский

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Exploring the potential of a newly constructed manganese peroxidase-producing yeast consortium for tolerating lignin degradation inhibitors while simultaneously decolorizing and detoxifying textile azo dye wastewater

Bioresource Technology, Journal Year: 2022, Volume and Issue: 351, P. 126861 - 126861

Published: Feb. 17, 2022

Language: Английский

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Oleaginous yeasts: Biodiversity and cultivation

Fungal Biology Reviews, Journal Year: 2022, Volume and Issue: 44, P. 100295 - 100295

Published: Dec. 5, 2022

Language: Английский

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A novel nutritional induction strategy flexibly switching the biosynthesis of food-like products from methane by a methanotrophic bacterium

Green Chemistry, Journal Year: 2024, Volume and Issue: 26(12), P. 7048 - 7058

Published: Jan. 1, 2024

By fine-tuning key nutrients in methanotrophic cultures, the production of cell proteins and carbohydrates can be artificially manipulated association with central metabolisms, enabling a flexible efficient biosynthesis CH 4 -derived products.

Language: Английский

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Biodiesel production from lignocellulosic biomass using Yarrowia lipolytica

Energy Conversion and Management X, Journal Year: 2021, Volume and Issue: 13, P. 100167 - 100167

Published: Dec. 20, 2021

Depletion of hydrocarbons is forcing to find alternative resources meet the energy demand growing population. Microbial biodiesel as a fuel can act cheaper and eco-friendly fossil fuel. Single-cell oil (SCO) consisting carbon, hydrogen oxygen grown over lignocellulosic biomass using oleaginous microorganisms are triacylglycerols which be converted biodiesel, with physicochemical properties similar conventional diesel. However, several cost-effective pretreatment methods required utilize biomasses. The current research study investigates SCO yield (and characteristics) obtained from sugarcane bagasse hydrolysate through various techniques. 4% v/v H2SO4 at 25 min ultra-sonication provided best depolymerisation results (based on glucose concentration). Yarrowia lipolytica was inoculated into hydrolysates, allowed grow °C, pH 6.5 rapid mixing for six days yielded 16.39 g/l. Biodiesel extracted via in-situ ex-situ transesterification. In-situ transesterification carried out catalyst K2CO3 yields 80% biodiesel. In comparison, 63% were achieved transesterification, where lipid extraction first step transesterified further in presence KOH obtain fatty acid methyl esters (FAME) subjected FTIR analysis, observed within international standards.

Language: Английский

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A comprehensive review on microbial lipid production from wastes: research updates and tendencies

Environmental Science and Pollution Research, Journal Year: 2023, Volume and Issue: 30(33), P. 79654 - 79675

Published: June 16, 2023

Language: Английский

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