Critical knowledge gaps and research needs related to the environmental dimensions of antibiotic resistance

Environment International, Journal Year: 2018, Volume and Issue: 117, P. 132 - 138

Published: May 7, 2018

There is growing understanding that the environment plays an important role both in transmission of antibiotic resistant pathogens and their evolution. Accordingly, researchers stakeholders world-wide seek to further explore mechanisms drivers involved, quantify risks identify suitable interventions. a clear value establishing research needs coordinating efforts within across nations order best tackle this global challenge. At international workshop late September 2017, scientists from 14 countries with expertise on environmental dimensions resistance gathered define critical knowledge gaps. Four key areas were identified where urgently needed: 1) relative contributions different sources antibiotics bacteria into environment; 2) environment, particularly anthropogenic inputs, evolution resistance; 3) overall human animal health impacts caused by exposure bacteria; 4) efficacy feasibility technological, social, economic behavioral interventions mitigate resistance.1.

Language: Английский

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Antibiotic Resistance in Bacteria—A Review

Antibiotics, Journal Year: 2022, Volume and Issue: 11(8), P. 1079 - 1079

Published: Aug. 9, 2022

A global problem of multi-drug resistance (MDR) among bacteria is the cause hundreds thousands deaths every year. In response to significant increase MDR bacteria, legislative measures have widely been taken limit or eliminate use antibiotics, including in form feed additives for livestock, but also metaphylaxis and its treatment, which was subject EU Regulation 2019/6. Numerous studies documented that both phenotypis gentic strategies enabling a natural defence against antibiotics induction mechanisms increasing used antibacterial chemicals. The presented this review developed by impact on reducing ability combat bacterial infections humans animals. Moreover, high prevalence multi-resistant strains environment ease transmission drug-resistance genes between different species commensal flora pathogenic like foodborne pathogens (E. coli, Campylobacter spp., Enterococcus Salmonella Listeria Staphylococcus spp.) favor rapid spread multi-resistance Given threat posed widespread phenomenon are dangerous animals, study presentation most frequent called as "foodborne pathoges" isolated from human order present significance related selected pathogens, especially those danger humans, publication presents statistical data percentage range occurrence drug various regions world. addition phenotypic characteristics pathogen resistance, detailed information detection specific groups antibiotics. It should be emphasized manuscript results own research i.e., E. coli Enetrococcus spp. This risks will contribute initiating implementing prevention development alternatives antimicrobials methods controlling bacteria.

Language: Английский

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Triclosan at environmentally relevant concentrations promotes horizontal transfer of multidrug resistance genes within and across bacterial genera

Environment International, Journal Year: 2018, Volume and Issue: 121, P. 1217 - 1226

Published: Oct. 30, 2018

Antibiotic resistance poses an increasing threat to public health. Horizontal gene transfer (HGT) promoted by antibiotics is recognized as a significant pathway disseminate antibiotic genes (ARGs). However, it unclear whether non-antibiotic, anti-microbial (NAAM) chemicals can directly promote HGT of ARGs in the environment.We aimed investigate triclosan (TCS), widely-used NAAM chemical personal care products, able stimulate conjugative multi-resistance carried plasmid within and across bacterial genera.We established two model mating systems, intra-genera inter-genera transfer. Escherichia coli K-12 LE392 carrying IncP-α RP4 was used donor, E. MG1655 or Pseudomonas putida KT2440 were intra- recipients, respectively. The mechanisms TCS unveiled detecting oxidative stress cell membrane permeability, combination with Nanopore sequencing, genome-wide RNA sequencing proteomic analyses.Exposure bacteria environmentally relevant concentrations (from 0.02 μg/L 20 μg/L) significantly stimulated plasmid-encoded genera. exposure ROS generation damaged membrane, caused increased expression SOS response regulatory umuC, dinB dinD donor. In addition, higher levels ATP synthesis encoding P. found dosage.TCS could enhance between triggering overproduction at concentrations. These findings improve our awareness hidden risks on spread resistance.

Language: Английский

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Biocidal Agents Used for Disinfection Can Enhance Antibiotic Resistance in Gram-Negative Species

Antibiotics, Journal Year: 2018, Volume and Issue: 7(4), P. 110 - 110

Published: Dec. 14, 2018

Biocidal agents used for disinfection are usually not suspected to enhance cross-resistance antibiotics. The aim of this review was therefore evaluate the effect 13 biocidal at sublethal concentrations on antibiotic resistance in Gram-negative species. A medline search performed each agent tolerance, resistance, horizontal gene transfer, and efflux pump. In cells adapted benzalkonium chloride a new most frequently found ampicillin (eight species), cefotaxime (six sulfamethoxazole (three some them with relevance healthcare-associated infections such as Enterobacter cloacae or Escherichia coli. With chlorhexidine often ceftazidime, imipenem species each) well tetracycline (seven each). Cross-resistance antibiotics also triclosan, octenidine, sodium hypochlorite, didecyldimethylammonium chloride. No has been described after low level exposure ethanol, propanol, peracetic acid, polyhexanide, povidone iodine, glutaraldehyde, hydrogen peroxide. Taking into account that disinfectants have no health benefit (e.g., alcohol-based hand rubs) but may cause it is obvious prefer products without them.

Language: Английский

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Intracellular versus extracellular antibiotic resistance genes in the environment: Prevalence, horizontal transfer, and mitigation strategies

Bioresource Technology, Journal Year: 2020, Volume and Issue: 319, P. 124181 - 124181

Published: Sept. 29, 2020

Language: Английский

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Antibiotic perturbations to the gut microbiome

Nature Reviews Microbiology, Journal Year: 2023, Volume and Issue: 21(12), P. 772 - 788

Published: July 25, 2023

Language: Английский

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Povidone Iodine: Properties, Mechanisms of Action, and Role in Infection Control and Staphylococcus aureus Decolonization

Antimicrobial Agents and Chemotherapy, Journal Year: 2020, Volume and Issue: 64(9)

Published: June 22, 2020

Nasal decolonization is an integral part of the strategies used to control and prevent spread methicillin-resistant Staphylococcus aureus (MRSA) infections. The two most commonly agents for are intranasal mupirocin 2% ointment chlorhexidine wash, but increasing emergence resistance treatment failure has underscored need alternative therapies. This article discusses povidone iodine (PVP-I) as agent based on literature reviewed during expert's workshop MRSA decolonization. Compared mupirocin, respectively, PVP-I 10 7.5% solutions demonstrated rapid superior bactericidal activity against in vitro ex vivo studies. Notably, 5% were also active both chlorhexidine-resistant mupirocin-resistant strains, respectively. Unlike available reports have not observed a link between induction bacterial or cross-resistance antiseptics antibiotics. These preclinical findings translate into clinical decolonization, where significantly improved efficacy wash was effective reducing surgical site infection orthopedic surgery. Overall, these qualities make it useful decolonizing prevention S. infections, additional experimental data required further evaluate use this setting.

Language: Английский

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Non-antibiotic pharmaceuticals promote the transmission of multidrug resistance plasmids through intra- and intergenera conjugation

The ISME Journal, Journal Year: 2021, Volume and Issue: 15(9), P. 2493 - 2508

Published: March 10, 2021

Antibiotic resistance is a global threat to public health. The use of antibiotics at sub-inhibitory concentrations has been recognized as an important factor in disseminating antibiotic via horizontal gene transfer. Although non-antibiotic, human-targeted pharmaceuticals are widely used by society (95% the market), potential contribution spread not clear. Here, we report that commonly consumed, non-antibiotic pharmaceuticals, including nonsteroidal anti-inflammatories (ibuprofen, naproxen, diclofenac), lipid-lowering drug (gemfibrozil), and β-blocker (propranolol), clinically environmentally relevant concentrations, significantly accelerated dissemination plasmid-borne bacterial conjugation. Various indicators were study response these drugs, monitoring reactive oxygen species (ROS) cell membrane permeability flow cytometry, arrangement, whole-genome RNA protein sequencing. Enhanced conjugation correlated well with increased production ROS permeability. Additionally, induced responses similar those detected when bacteria exposed antibiotics, such inducing SOS enhancing efflux pumps. findings advance understanding transfer genes, emphasizing concern enhance among populations.

Language: Английский

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Environmental contamination in a high-income country (France) by antibiotics, antibiotic-resistant bacteria, and antibiotic resistance genes: Status and possible causes

Environment International, Journal Year: 2021, Volume and Issue: 159, P. 107047 - 107047

Published: Dec. 16, 2021

Antimicrobial resistance (AMR) is a major global public health concern, shared by large number of human and animal actors. Within the framework One Health approach, actions should be implemented in environmental realm, as well realms. The Government France commissioned report to provide policy decision makers with an evidential basis for recommending or taking future mitigate AMR environment. We first examined mechanisms that underlie emergence persistence antimicrobial This drew up inventory contamination aquatic terrestrial environments antibiotics, anticipating findings will representative some other high-income countries. Effluents wastewater treatment plants were identified source on French territory, spreading organic waste products more diffuse incidental environments. A limitation this review heterogeneity available data space time, lack certain sources. Comparing Measured Environmental Concentrations (MECs) predicted no effect concentrations (PNECs), fluoroquinolones trimethoprim representing high medium risk favoring selection resistant bacteria treated most contaminated rivers. All antibiotic molecules analyzed (erythromycin, clarithromycin, azithromycin, tetracycline) at low those However, impairs their full exploitation. Consequently, we listed indicators survey antibiotics environment recommended harmonization sampling strategies endpoints analyses. Finally, objectives methods used present work could comprise useful example how national authorities countries sharing common socio-geographic characteristics seek better understand define dimension particular settings.

Language: Английский

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Occurrence and removal of antibiotics from industrial wastewater

Environmental Chemistry Letters, Journal Year: 2021, Volume and Issue: 19(2), P. 1477 - 1507

Published: Jan. 3, 2021

Language: Английский

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