Unravelling the Portuguese Coastal and Transitional Waters’ Microbial Resistome as a Biomarker of Differential Anthropogenic Impact DOI Creative Commons
Bernardo Duarte, Andreia Figueiredo, Patrício Ramalhosa

et al.

Toxics, Journal Year: 2022, Volume and Issue: 10(10), P. 613 - 613

Published: Oct. 15, 2022

Portugal mainland and Atlantic archipelagos (Madeira Azores) provide a wide array of coastal ecosystems with varying typology degrees human pressure, which shape the microbial communities thriving in these habitats, leading to development resistance traits. The samples collected on Portuguese northeast coast waters show an unequivocal prevalence Bacteria over Archaea high Proteobacteria, Cyanobacteria, Bacteroidetes Actinobacteria. Several taxa, such as Vibrio genus, showed significant correlations anthropogenic pollution. These pressures, along differences species diversity among surveyed sites, lead observed presence resistance-related sequences’ abundance (set all metal antibiotic resistant genes their precursors pathogenic non-pathogenic bacteria). Gene ontology terms resistance, redox regulation oxidative stress response were prevalent. A higher number found between sequences pollution, inorganic pressures density nearby population centres when compared taxa at different phylogenetic levels same environmental This points towards predominance conditions sequence rather than abundance. Our data suggest that whole resistome profile can more relevant or integrative answers disturbance environment, either grouped gene groups, appearing promising tool for impact assessment studies which, due ubiquity across microbes, be independently present samples.

Language: Английский

Fatty acid profiles as natural tracers of provenance and lipid quality indicators in illegally sourced fish and bivalves DOI
Vanessa F. Fonseca, Irina A. Duarte, Ana Rita Matos

et al.

Food Control, Journal Year: 2021, Volume and Issue: 134, P. 108735 - 108735

Published: Nov. 28, 2021

Language: Английский

Citations

32
Biomarker Studies in Stress Biology: From the Gene to Population, from the Organism to the Application DOI Creative Commons
Marco F.L. Lemos

Biology, Journal Year: 2021, Volume and Issue: 10(12), P. 1340 - 1340

Published: Dec. 16, 2021

Endpoints assessed at the population or community level are most often result of sum effects on individuals, arising from cellular and molecular levels. Within this framework, these lower biological endpoints more responsive an early stage exposure, making them potential toolboxes to be used as early-warning markers address stress. Given this, by linking responses understanding organisms' metabolism physiology, possibilities for use biomarkers in stress biology vast. Here, biomarker comprehensive examples given enlighten need link levels organization, their usefulness a myriad fields applications is presented discussed.

Language: Английский

Citations

31
Roadmap to sustainably develop the European seaweed industry DOI Creative Commons
Alexander Jueterbock, Bernardo Duarte, Ricardo Melo

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: March 12, 2025

Abstract How to build a sustainable seaweed industry is important in Europe’s quest produce 8 million tons of by 2030. Interviews with representatives suggest that business models focused only on financial gain would fail. As team interdisciplinary experts, we offer roadmap satisfies the increasing demand for practices leveraging synergies existing industries as European develops beyond experimental cultivation.

Language: Английский

Citations

0
Written in ink: Elemental signatures in octopus ink successfully trace geographical origin DOI
Bernardo Duarte, João Carreiras, Renato Mamede

et al.

Journal of Food Composition and Analysis, Journal Year: 2022, Volume and Issue: 109, P. 104479 - 104479

Published: March 11, 2022

Language: Английский

Citations

15
Fatty acids as bioindicators of organohalogen exposure in marine fish from a highly polluted estuary: First insight into small-scale regional differences DOI
Fei Liu, Qiang Xie,

Ri-Qing Yu

et al.

Journal of Hazardous Materials, Journal Year: 2023, Volume and Issue: 452, P. 131337 - 131337

Published: March 31, 2023

Language: Английский

Citations

8
Quinoa variety identification based on fatty acid composition and multivariate chemometrics approaches DOI Creative Commons
Bernardo Duarte, Johannes W. Goessling, Vanessa F. Fonseca

et al.

Journal of Food Composition and Analysis, Journal Year: 2022, Volume and Issue: 114, P. 104798 - 104798

Published: July 27, 2022

Quinoa consumption has increased in worldwide importance due to an extraordinary nutritional value and public acceptance as alternative food. Fatty acid profiles of 10 quinoa varieties grown the same geographical location were analyzed using different chemometric multivariate approaches [variable partial least square discriminant analysis (VIP-PLS-DA), stepwise linear (S-LDA), (LDA), random forests (RF) canonical principal components (CAP)]. The application variable selection such S-LDA LDA significantly classification accuracy (78% 74% respectively) samples according their variety. approach allowed reduce number selected fatty acids, representing those acids with higher statistical significance when applying other non-random approaches. These also estimation lipid each variety for suitability human diet, providing insights into various qualities It is proposed that these results can be used facilitate optimized economic value.

Language: Английский

Citations

12
Persistent Organic Pollutants in Tagus Estuary Salt Marshes: Patterns of Contamination and Plant Uptake DOI Creative Commons
Ricardo Cruz de Carvalho, João Cardoso, João Carreiras

et al.

Journal of Xenobiotics, Journal Year: 2024, Volume and Issue: 14(3), P. 1165 - 1186

Published: Sept. 2, 2024

The presence of anthropogenic compounds, including organochlorine pesticides (OCPs) and polychlorinated biphenyls (PCBs), was studied in three salt marshes within the Tagus estuary, Portugal, along an pressure gradient. Results revealed differences OCPs PCBs among marshes, with differing concentration levels. Specifically, one marsh, surrounding agricultural activity, showed highest OCP concentrations, while another, a historical industrial past, exhibited elevated PCB In contrast, third part natural reserve, displayed comparatively lower concentrations both substances. Sediment likely influenced by practices, were found to be comparable or higher than those observed other Portuguese estuaries. halophyte Spartina maritima absorb OCPs, particularly its aboveground tissues, suggesting bioaccumulation plant. Additionally, levels appeared history, marsh displaying notably concentrations. conclusion, persistence compounds ecosystems notwithstanding regulatory prohibitions implemented 1990s highlights need for continuous monitoring study such sites necessity remediation which are imperative mitigate ecological health risks these polluted marshes.

Language: Английский

Citations

2
Impacts of dissolved Zn and nanoparticle forms in the fatty acid landscape of Mytilus galloprovincialis DOI
Joana Roma, Eduardo Feijão, Catarina Vinagre

et al.

The Science of The Total Environment, Journal Year: 2022, Volume and Issue: 817, P. 152807 - 152807

Published: Jan. 10, 2022

Language: Английский

Citations

10
Trust your seaweeds: Fine-scale multi-elemental traceability of edible seaweed species harvested within an estuarine system DOI
Bernardo Duarte, Renato Mamede, Isabel Caçador

et al.

Algal Research, Journal Year: 2023, Volume and Issue: 70, P. 102975 - 102975

Published: Jan. 13, 2023

Language: Английский

Citations

6
Elemental Chemometrics as Tools to Depict Stalked Barnacle (Pollicipes pollicipes) Harvest Locations and Food Safety DOI Creative Commons
Bernardo Duarte, Renato Mamede, Irina A. Duarte

et al.

Molecules, Journal Year: 2022, Volume and Issue: 27(4), P. 1298 - 1298

Published: Feb. 15, 2022

The stalked barnacle Pollicipes pollicipes is an abundant species on the very exposed rocky shore habitats of Spanish and Portuguese coasts, constituting also important economical resource, as a seafood item with high commercial value. Twenty-four elements were measured by untargeted total reflection X-ray fluorescence spectroscopy (TXRF) in edible peduncle barnacles sampled six sites along western coast, comprising 90 individuals. elemental profile individuals originated from several geographical (N = 15 per site), analysed using chemometric multivariate approaches (variable importance partial least square discriminant analysis (VIP-PLS-DA), stepwise linear (S-LDA), (LDA), random forests (RF) canonical principal components (CAP)), to evaluate ability each approach trace origin animals collected. As suspension feeder, this introduces degree background noise, leading comparatively lower classification based complete (canonical analysis). application variable selection such VIP-PLS-DA S-LDA significantly increased accuracy (77.8% 84.4%, respectively) samples according their harvesting area, while reducing number needed for classification, thus noise. Moreover, selected are similar those other non-random approaches, reinforcing reliability selection. This analytical procedure allowed depict risk, terms human consumption these animals, highlighting areas where delicacies presented values critical compared standard thresholds consumption.

Language: Английский

Citations

9