medRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2022,
Volume and Issue:
unknown
Published: Nov. 24, 2022
Abstract
During
the
COVID-19
pandemic,
wastewater-based
surveillance
has
been
used
alongside
diagnostic
testing
to
monitor
infection
rates.
With
decline
in
cases
reported
public
health
departments
due
at-home
testing,
wastewater
data
may
serve
as
primary
input
for
epidemiological
models,
but
training
these
models
is
not
straightforward.
We
explored
factors
affecting
noise
and
bias
ratio
between
case
collected
26
sewersheds
California
from
October
2020
March
2022.
The
strength
of
relationship
appeared
dependent
on
sampling
frequency
population
size,
was
increased
by
normalization
flow
rate
or
count
Additionally,
lead
lag
times
varied
over
time
space,
log-transformed
individual
concentrations
changed
time.
This
sequentially
Epsilon/Alpha,
Delta,
Omicron
BA.1
variant
surges
also
related
rate.
Based
this
analysis,
we
present
a
framework
scenarios
describing
dynamics
aid
handling
decisions
ongoing
modeling
efforts.
The Science of The Total Environment,
Journal Year:
2022,
Volume and Issue:
865, P. 161196 - 161196
Published: Dec. 26, 2022
Over
the
course
of
Corona
Virus
Disease-19
(COVID-19)
pandemic
in
2020–2022,
monitoring
severe
acute
respiratory
syndrome
coronavirus
2
ribonucleic
acid
(SARS-CoV-2
RNA)
wastewater
has
rapidly
evolved
into
a
supplementary
surveillance
instrument
for
public
health.
Short
term
trends
(2
weeks)
are
used
as
basis
policy
and
decision
making
on
measures
dealing
with
pandemic.
Normalisation
is
required
to
account
dilution
rate
domestic
that
can
strongly
vary
due
time-
location-dependent
sewer
inflow
runoff,
industrial
discharges
extraneous
waters.
The
standard
approach
sewage
normalisation
using
flow
measurements,
although
based
not
effective
case
volume
sampled
does
match
produced.
In
this
paper,
two
alternative
methods,
electrical
conductivity
crAssphage
have
been
studied
compared
measurements.
For
this,
total
1116
24-h
flow-proportional
samples
collected
between
September
2020
August
2021
at
nine
locations.
addition,
221
stool
analysed
determine
daily
load
per
person.
Results
show
that,
shedding
rates
person
greatly,
population-level
loads
day
were
constant
over
time
similar
all
catchments.
Consequently,
be
quantitative
biomarker
populations
above
5595
persons.
Electrical
particularly
suitable
relative
dry
weather
concentrations.
overall
conclusion
necessary
reliably
short-term
virus
circulation,
enhanced
and/or
measurement
quality
check.
The Science of The Total Environment,
Journal Year:
2023,
Volume and Issue:
871, P. 162069 - 162069
Published: Feb. 7, 2023
During
the
COVID-19
pandemic,
wastewater-based
surveillance
has
been
used
alongside
diagnostic
testing
to
monitor
infection
rates.
With
decline
in
cases
reported
public
health
departments
due
at-home
testing,
wastewater
data
may
serve
as
primary
input
for
epidemiological
models,
but
training
these
models
is
not
straightforward.
We
explored
factors
affecting
noise
and
bias
ratio
between
case
collected
26
sewersheds
California
from
October
2020
March
2022.
The
strength
of
relationship
appeared
dependent
on
sampling
frequency
population
size,
was
increased
by
normalization
flow
rate
or
count
Additionally,
lead
lag
times
varied
over
time
space,
log-transformed
individual
concentrations
changed
time.
This
decreased
Epsilon/Alpha
Delta
variant
surges
during
Omicron
BA.1
surge,
also
related
rate.
Based
this
analysis,
we
present
a
framework
scenarios
describing
dynamics
aid
handling
decisions
ongoing
modeling
efforts.
Environmental Science & Technology,
Journal Year:
2023,
Volume and Issue:
57(35), P. 12969 - 12980
Published: Aug. 23, 2023
Wastewater-based
testing
(WBT)
for
SARS-CoV-2
has
rapidly
expanded
over
the
past
three
years
due
to
its
ability
provide
a
comprehensive
measurement
of
disease
prevalence
independent
clinical
testing.
The
development
and
simultaneous
application
WBT
measured
biomarkers
research
activities
pursuit
public
health
goals,
both
areas
with
well-established
ethical
frameworks.
Currently,
practitioners
do
not
employ
standardized
review
process,
introducing
potential
adverse
outcomes
professionals
community
members.
To
address
this
deficiency,
an
interdisciplinary
workshop
developed
framework
structured
WBT.
employed
consensus
approach
create
as
set
11
questions
derived
from
primarily
guidance.
This
study
retrospectively
applied
these
monitoring
programs
covering
emergent
phase
pandemic
(3/2020-2/2022
(
Microorganisms,
Journal Year:
2022,
Volume and Issue:
10(3), P. 659 - 659
Published: March 19, 2022
The
last
two
years
have
been
marked
by
the
severe
acute
respiratory
syndrome
coronavirus
2
(SARS-CoV-2)
pandemic.
This
virus
is
found
in
intestinal
tract;
it
reaches
wastewater
systems
and,
consequently,
natural
receiving
water
bodies.
As
such,
inefficiently
treated
(WW)
can
be
a
means
of
contamination.
currently
used
methods
for
disinfection
WW
lead
to
formation
toxic
compounds
and
expensive
or
inefficient.
new
alternative
approaches
must
considered,
namely,
photodynamic
inactivation
(PDI).
In
this
work,
bacteriophage
φ6
(or,
simply,
phage
φ6),
which
has
as
suitable
model
enveloped
RNA
viruses,
such
coronaviruses
(CoVs),
was
SARS-CoV-2.
Firstly,
understand
virus's
survival
environment,
subjected
different
laboratory-controlled
environmental
conditions
(temperature,
pH,
salinity,
solar
UV-B
irradiation),
its
persistence
over
time
assessed.
Second,
assess
efficiency
PDI
towards
virus,
assays
were
performed
both
phosphate-buffered
saline
(PBS),
commonly
aqueous
matrix,
secondarily
(a
real
matrix).
Third,
generally
discharged
into
marine
environment
after
treatment,
safety
PDI-treated
assessed
through
determination
viability
native
microorganisms
their
contact
with
effluent.
Overall,
results
showed
that,
when
surrogate
SARS-CoV-2,
remains
viable
considerable
period.
Moreover,
proved
an
efficient
approach
effluent
no
toxicity
aquatic
under
realistic
dilution
conditions,
thus
endorsing
safe
tertiary
method.
Although
all
studies
φ6,
considered
further
using
SARS-CoV-2
are
necessary;
nevertheless,
findings
show
potential
controlling
WW.
PLoS ONE,
Journal Year:
2023,
Volume and Issue:
18(8), P. e0289343 - e0289343
Published: Aug. 3, 2023
During
the
COVID-19
pandemic,
wastewater-based
surveillance
has
been
shown
to
be
a
useful
tool
for
monitoring
spread
of
disease
in
communities
and
emergence
new
viral
variants
concern.
As
pandemic
enters
its
fourth
year
clinical
testing
declined,
wastewater
offers
consistent
non-intrusive
way
monitor
community
health
long
term.
This
study
sought
understand
how
accurately
represented
actual
burden
between
communities.
Two
varying
size
demographics
Michigan
were
monitored
SARS-CoV-2
March
2020
February
2022.
Additionally,
each
was
concern
from
December
Wastewater
results
compared
with
zipcode
county
level
case
data
determine
which
scope
most
correlated
loading.
Pearson
r
correlations
highest
smaller
two
(population
25,000)
N1
GC/person/day
data,
date
onset
symptoms
(
=
0.81).
A
clear
difference
seen
more
cases
virus
signals
larger
110,000)
when
examined
based
on
vaccine
status,
reached
only
50%.
While
levels
had
lower
correlation
community,
information
still
as
valuable
supporting
public
actions
further
including
vaccination
status
should
future.
