Melatonin and resveratrol alleviate molecular and metabolic toxicity induced by Bisphenol A in endometrial organoids

Reproductive Toxicology, Journal Year: 2024, Volume and Issue: 128, P. 108628 - 108628

Published: June 5, 2024

Bisphenol A (BPA), a widespread environmental contaminant, poses concerns due to its disruptive effects on physiological functions of the uterine endometrium. In contrast, melatonin (MT) and Resveratrol (RSV) are under scrutiny for their potential protective roles against BPA-induced damage. For efficacy ethical in animal test, endometrial organoids, three-dimensional models mimicking endometrium, serve as crucial tools unraveling impact factors reproductive health. This study aimed comprehensively characterize morphological, molecular metabolic responses porcine organoids BPA assess MT RSV. Porcine uteri were prepared, digested with collagenase, mixed Matrigel, incubated at 38°C 5 % CO

Language: Английский

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Algae-based biotransformation of COVID-19 antiviral ribavirin: Insight on metabolic effect, wastewater application and biological toxicity

Bioresource Technology, Journal Year: 2025, Volume and Issue: unknown, P. 132390 - 132390

Published: March 1, 2025

Language: Английский

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PFHxA and PFHxS promote breast cancer progression in 3D culture: MEX3C-associated immune infiltration revealed by bioinformatics and machine learning

Journal of Hazardous Materials, Journal Year: 2025, Volume and Issue: 494, P. 138458 - 138458

Published: May 2, 2025

Language: Английский

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Protective role of fetal bovine serum on PLHC-1 spheroids exposed to a mixture of plastic additives: A lipidomic perspective

Toxicology in Vitro, Journal Year: 2024, Volume and Issue: 96, P. 105771 - 105771

Published: Jan. 3, 2024

The use of fetal bovine serum (FBS) in cell culture is being questioned for scientific and ethical reasons, prompting the exploration alternative approaches. Nevertheless, influence FBS on functioning, especially fish cells, has not been comprehensively examined. This study aims to evaluate impact lipidome PLHC-1 spheroids investigate cellular molecular responses plastic additives presence/absence FBS. Lipidomic analyses were conducted using liquid chromatography coupled with a high-resolution quadrupole time-of-flight mass spectrometer (HRMS-QToF). removal from medium 24 h significantly changed lipid profile spheroids, resulting depletion cholesterol esters (CEs), phosphatidylcholines (PCs) lyso-phosphatidylcholines (LPCs), while ceramides certain glycerophospholipids slightly increased. Additionally, exclusion led increased cytotoxicity caused by mixture lipidomic alterations, including an elevation ceramides. emphasizes protective role components liver against underscores importance considering exposure conditions when studying metabolomic toxicants.

Language: Английский

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An in-depth examination of Per- and Polyfluoroalkyl (PFAS) effects on transporters, with emphasis on the ABC superfamily: A critical review

Toxicology, Journal Year: 2024, Volume and Issue: 508, P. 153901 - 153901

Published: Aug. 1, 2024

Language: Английский

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A Novel “Cells-on-Particles” Cytotoxicity Testing Platform In vitro: Design, Characterization, and Validation against Engineered Nanoparticle Aerosol

Toxicology, Journal Year: 2024, Volume and Issue: 508, P. 153936 - 153936

Published: Aug. 30, 2024

Language: Английский

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In vitro hepatic 3D cell models and their application in genetic toxicology: a systematic review

Mutation Research/Genetic Toxicology and Environmental Mutagenesis, Journal Year: 2024, Volume and Issue: 900, P. 503835 - 503835

Published: Nov. 1, 2024

The rapid development of new chemicals and consumer products has raised concerns about their potential genotoxic effects on human health, including DNA damage leading to serious diseases. For such pharmaceutical products, international regulations require genotoxicity data, initially obtained through in vitro tests, followed by vivo experiments, if needed. Traditionally, laboratory animals have been used for this purpose, however, they are costly, ethically problematic, often unreliable due species differences. Therefore, innovative more accurate testing approaches rapidly being developed replace, refine reduce (3R) the use experimental purposes improve relevance humans toxicology studies. One three-dimensional (3D) cell models, which already highlighted as superior alternatives two-dimensional (2D) cultures that traditionally models safety pharmaceuticals. 3D provide physiologically relevant information predictive data conditions. In review article, we a comprehensive overview hepatic HepG2, HepG2/C3A, HepaRG, primary hepatocytes, iPSC-derived application field genotoxicology. Through detailed literature analysis, identified 31 studies conducted between 2007 April 2024 variety standard methods, comet assay, micronucleus γH2AX well methodological approaches, toxicogenomics, assess cytotoxic activity chemicals, nanoparticles natural toxins. Based our search, can conclude increasing over years an even greater future implementation further refinement genetic risk assessment.

Language: Английский

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Chlorinated Anthracenes Induced Pulmonary Immunotoxicity in 3D Coculture Spheroids Simulating the Lung Microenvironment

Environmental Science & Technology, Journal Year: 2024, Volume and Issue: 58(27), P. 11923 - 11934

Published: June 25, 2024

Chlorinated anthracenes (Cl-Ants), persistent organic pollutants, are widely detected in the environment, posing potential lung toxicity risks due to frequent respiratory exposure. However, direct evidence and a comprehensive understanding of their mechanisms lacking. Building on our prior findings Cl-Ants' immunotoxic risks, this study developed three-dimensional coculture spheroid model mimicking lung's immune microenvironment. The objective is explore pulmonary immunotoxicity comprehend its mechanisms, taking into account heightened reactivity exposure Cl-Ants. results demonstrated that Cl-Ants led reduced size, increased macrophage migration outward, lowered cell viability, elevated 8-OHdG levels, disturbed anti-infection balance, altered cytokine production. Specifically, chlorine substituent number correlates with extent disruption indicators caused by Cl-Ants, stronger effects observed dichlorinated Ant compared those monochlorinated Ant. Furthermore, we identified critical regulatory genes associated viability (ALDOC ALDOA), bacterial response (TLR5 MAP2K6), GM-CSF production (CEBPB). Overall, offers initial vitro low-dose Cl-PAHs' immunotoxicity, advancing structure-related improving external assessment methods for environmental which holds significance future monitoring evaluation.

Language: Английский

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Longitudinal tracking of perfluorooctanoic acid exposure on mammary epithelial cell spheroids by dynamic optical coherence tomography

Biomedical Optics Express, Journal Year: 2024, Volume and Issue: 15(9), P. 5115 - 5115

Published: July 31, 2024

We investigated the morphology and intracellular motility of mammary epithelial cell (MCF10DCIS.com) spheroids cultured in 3D artificial extracellular matrix under perfluorooctanoic acid (PFOA) exposure. Dynamic optical coherence tomography (OCT) was employed for real-time, non-invasive imaging these longitudinally over 12 days PFOA exposures up to 500 µM. Despite no significant changes volume or asphericity spheroids, morphological alterations were observed OCT images at 100 µM on Day from 4 Intracellular assessed by inverse-power-law exponent speckle fluctuation spectrum ( α ), an autocorrelation-based amplitude M ). Linear regression indicated that both concentration culture time are highly predictors p < 0.001 all). Both have positive associations with negative association , where increased indicates suppression higher frequency fluctuations (∼> 2 Hz) relative those lower frequencies, decreased overall motility. This study can lead future development biomarkers per- polyfluoroalkyl substances (PFAS) exposure using dynamic its associated toolkit quantitative metrics.

Language: Английский

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Cytotoxicity of the exhaled aerosol particles from the usage of conventional cigarette and heated tobacco product as determined by a novel “Cells-on-Particles” exposure model in vitro

Environmental Pollution, Journal Year: 2024, Volume and Issue: 361, P. 124870 - 124870

Published: Aug. 30, 2024

Language: Английский

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