Identification and Validation of Cuproptosis-Related LncRNA Signatures in the Prognosis and Immunotherapy of Clear Cell Renal Cell Carcinoma Using Machine Learning

Biomolecules, Journal Year: 2022, Volume and Issue: 12(12), P. 1890 - 1890

Published: Dec. 16, 2022

(1) Objective: We aimed to mine cuproptosis-related LncRNAs with prognostic value and construct a corresponding model using machine learning. External validation of the was performed in ICGC database multiple renal cancer cell lines via qPCR. (2) Methods: TCGA cohorts related clear carcinoma were included. GO KEGG analyses conducted determine biological significance differentially expressed (CRLRs). Machine learning (LASSO), Kaplan–Meier, Cox genes. The tumor microenvironment mutation load further studied. TIDE IC50 used evaluate response immunotherapy, risk cuproptosis genes established, ability this verified an external independent cohort. identified normal HK-2 cells four (3) Results: obtained 280 CRLRs 66 included TCGA-KIRC Then, three hub (AC026401.3, FOXD2−AS1, LASTR), which over-expressed ccRCC compared human cortex proximal tubule epithelial line HK-2, identified. In database, expression FOXD2-AS1 LASTR consistent qPCR TCGA-KIRC. results also indicated that patients low-risk ccRCC—stratified by tumor-node metastasis stage, sex, grade—had significantly better overall survival than those high-risk ccRCC. predictive algorithm showed that, according CRLR models, group more sensitive nine target drugs (A.443654, A.770041, ABT.888, AG.014699, AMG.706, ATRA, AP.24534, axitinib, AZ628), based on estimated half-maximal inhibitory concentrations. contrast, ABT.263 AKT inhibitors VIII AS601245. Using correlation between immune immunotherapy revealed are likely respond patients. terms marker levels, there significant differences high- groups. A high TMB score associated worse survival, could be factor for KIRC. (4) Conclusions: This study elucidates core LncRNAs, AC026401.3, LASTR, potential value, immunotherapeutic strategy, outcome

Language: Английский

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Epigenetic regulation of cutaneous T-cell lymphoma is mediated by dysregulated lncRNA MALAT1 through modulation of tumor microenvironment

Frontiers in Oncology, Journal Year: 2022, Volume and Issue: 12

Published: Aug. 17, 2022

Cutaneous T-Cell Lymphoma (CTCL) is a rare non-Hodgkin lymphoma marked by migration of T-lymphocytes to the skin. It has many subtypes some which are aggressive with documented metastasis. We investigated possible role lncRNA MALAT1 in CTCL cells because its involvement cancer A screening patients revealed elevated levels patients, compared healthy individuals. For our investigation, we employed HH and H9 silenced understand mediated functions. Such silencing resulted reversal EMT inhibition stem cell phenotype, along reduced growth proliferation. was established through increased E-cadherin N-cadherin while phenotype evident Sox2 Nanog. had higher circulating IL-6, IL-8, IL-10, TGFβ, PGE2 MMP7 factors released tumor-associated macrophages tumor microenvironment. sponged miR-124 as this suppressive miRNA de-repressed upon silencing. Moreover, downregulation attenuated effects. Our study provides rationale for further studies focused on an evaluation MALAT1-miR-124 progression.

Language: Английский

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Long non-coding RNAs play an important regulatory role in tumorigenesis and tumor progression through aerobic glycolysis

Frontiers in Molecular Biosciences, Journal Year: 2022, Volume and Issue: 9

Published: Aug. 22, 2022

Compared to normal cells, cancer cells generate ATP mainly through aerobic glycolysis, which promotes tumorigenesis and tumor progression. Long non-coding RNAs (LncRNAs) are a class of transcripts longer than 200 nucleotides with little or without evident protein-encoding function. LncRNAs involved in the ten hallmarks cancer, interestingly, they also closely associated glycolysis. However, mechanism this process is non-transparent date. Demonstrating lncRNAs regulating progression glycolysis particularly critical for therapy, may provide novel therapeutic targets strategies treatment. In review, we discuss role progression, further explore their interaction, hope target

Language: Английский

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A novel lncRNA FPASL regulates fibroblast proliferation via the PI3K/AKT and MAPK signaling pathways in hypertrophic scar

Acta Biochimica et Biophysica Sinica, Journal Year: 2022, Volume and Issue: unknown

Published: Aug. 29, 2022

Hypertrophic scar is a problem for numerous patients, especially after burns, and characterized by increased fibroblast proliferation collagen deposition. Increasing evidence demonstrates that lncRNAs contribute to the development progression of various diseases. However, function in hypertrophic formation remains poorly characterized. In this study, novel proliferation-associated lncRNA, named lncRNA FPASL (MSTRG.389905.1), which mainly localized cytoplasm, found be downregulated scar, as detected microarray qRT-PCR. The full-length further investigation confirms it has no protein-coding potential. knockdown fibroblasts triggers proliferation, whereas overexpression directly attenuates fibroblasts. Furthermore, target genes differentially expressed scars matched adjacent normal tissues are enriched signaling pathways, including PI3K/AKT MAPK determined GO annotation KEGG enrichment analysis. We also demonstrate activates specific inhibitors pathways can reverse promoted knockdown. Our findings better understanding role suggest may act potential therapeutic scar.

Language: Английский

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LncRNA SNHG25 Promotes Glioma Progression Through Activating MAPK Signaling

Molecular Neurobiology, Journal Year: 2022, Volume and Issue: 59(11), P. 6993 - 7005

Published: Sept. 7, 2022

Language: Английский

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